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Information on EC 3.4.24.25 - vibriolysin

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.25 vibriolysin
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This record set is specific for:
UNIPROT: A1DRD5 not found.
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Preferential cleavage of bonds with bulky hydrophobic groups in P2 and P1'. Phe at P1' is the most favoured residue, which distinguished this enzyme from thermolysin
Synonyms
vibriolysin, ha/protease, mcp-02, aeromonas neutral protease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Aeromonas neutral protease
-
-
-
-
Aeromonas proteolytica neutral proteinase
-
-
-
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Aeromonolysin
-
-
-
-
Proteinase, Aeromonas proteolytica neutral
-
-
-
-
Vibriolysin
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
58500-42-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(2-furylacryloyl)glycyl-L-leucine + H2O
?
show the reaction diagram
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
?
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.32
3-(2-furylacryloyl)glycyl-L-leucine
at 25°C, at pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
using casein as substrate, at 60°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
using casein as substrate, at pH 8.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A1DRD5_PSEU9
Pseudoalteromonas sp. (strain SM9913)
727
0
78276
TrEMBL
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55 - 64
the melting temperature is at 64°C, the half-life at 55°C is 90 min, the half-life at 60°C is 23 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-Sepharose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xie, B.B.; Bian, F.; Chen, X.L.; He, H.L.; Guo, J.; Gao, X.; Zeng, Y.X.; Chen, B.; Zhou, B.C.; Zhang, Y.Z.
Cold adaptation of zinc metalloproteases in the thermolysin family from deep sea and arctic sea ice bacteria revealed by catalytic and structural properties and molecular dynamics: new insights into relationship between conformational flexibility and hydr
J. Biol. Chem.
284
9257-9269
2009
Pseudoalteromonas sp. SM495 (B6E623), Pseudoalteromonas sp. SM9913 (A1DRD5)
Manually annotated by BRENDA team