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Information on EC 3.4.24.20 - peptidyl-Lys metalloendopeptidase and Organism(s) Pleurotus ostreatus and UniProt Accession P81055

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.20 peptidyl-Lys metalloendopeptidase
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Select one or more organisms in this record: ?
This record set is specific for:
Pleurotus ostreatus
UNIPROT: P81055 not found.
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Word Map
The taxonomic range for the selected organisms is: Pleurotus ostreatus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Preferential cleavage in proteins: -Xaa-/-Lys- (in which Xaa may be Pro)
Synonyms
lys-n, gfmep, pomep, peptidyl-lys metalloendopeptidase, peptidyl-lys metallopeptidase, am-lysn, aspzincin metalloendopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Armillaria mellea neutral proteinase
-
-
-
-
GFMEP
-
-
-
-
MEP
-
-
-
-
Peptidyllysine metalloproteinase
-
-
-
-
POMEP
-
-
-
-
Proteinase, peptidyllysine metallo-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
65979-41-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl-Ser-Thr-Ala-Thr-Lys-Leu-Ser-Trp + H2O
?
show the reaction diagram
fluorescent peptide substrate
-
?
azocasein + H2O
?
show the reaction diagram
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
Bovine insulin B-chain + H2O
?
show the reaction diagram
-
cleavage site: Pro29-Lys30, yielding 2 fragments: dipeptide Lys-Ala and substrate devoid of 2 C-terminal amino acids
-
-
?
Equine heart cytochrome c + H2O
?
show the reaction diagram
-
-
-
-
?
Polyarginine + H2O
?
show the reaction diagram
-
-
-
-
?
Polylysine + H2O
?
show the reaction diagram
-
best substrate
-
-
?
protamine sulfate + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
can substitute for Zn2+, enhances activity
Zn2+
zinc-metalloendopetidase
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,2'-bipyridine
-
1 mM, weak
2-mercaptoethanol
-
not
Ag2+
-
1 mM, strong
antipain
-
not
dithiothreitol
Guanidine-HCl
-
2 M and above, not at 1 M
N-ethylmaleimide
-
weak, caseinolytic activity
p-chloromercuribenzoate
-
weak, caseinolytic activity
phosphoramidon
-
not
SDS
-
0.1% and above, not at 0.01%
tosyl-Lys chloromethyl ketone
-
weak, caseinolytic
Urea
-
5 M, not at 2 M
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
azocasein hydrolysis
8.5
rather active with fluorogenic peptide substrates than at pH 5.0
5.6
-
azocoll or azocasein as substrate
additional information
-
pI: 8.35
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
edible mushroom
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PLMP_PLEOS
168
0
17925
Swiss-Prot
Mitochondrion (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
1 * 18000, Pleurotus ostreatus, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 18000, Pleurotus ostreatus, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
18 h, 10% loss of caseinolytic activity at 4°C
31048
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
15 min, 10% loss of caseinolytic activity
70
-
15 min, inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Stable to 5 h at 37°C in 0.1 M acetate buffer, pH 5, containing 2 M urea, 1 M guanidine-HCl or 0.01% SDS, inactivation at higher concentrations of urea, guanidine-HCl or SDS
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dohmae, N.; Hayashi, K.; Miki, K.; Tsumuraya, Y.; Hashimoto, Y.
Purification and characterization of intracellular proteinases in Pleurotus ostreatus fruiting bodies
Biosci. Biotechnol. Biochem.
59
2074-2080
1995
Pleurotus ostreatus
Manually annotated by BRENDA team
Nonaka, T.; Hashimoto, Y.; Takio, K.
Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotus ostreatus fruiting bodies
J. Biochem.
124
157-162
1998
Grifola frondosa (P81054), Grifola frondosa, Pleurotus ostreatus (P81055), Pleurotus ostreatus
Manually annotated by BRENDA team