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Information on EC 3.4.23.32 - Scytalidopepsin B and Organism(s) Scytalidium lignicola and UniProt Accession P15369

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.32 Scytalidopepsin B
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Select one or more organisms in this record: ?
This record set is specific for:
Scytalidium lignicola
UNIPROT: P15369 not found.
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Word Map
The taxonomic range for the selected organisms is: Scytalidium lignicola
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Hydrolysis of proteins with broad specificity, cleaving Phe24-/-Phe, but not Leu15-Tyr and Phe25-Tyr in the B chain of insulin
Synonyms
eqolisin, scytalidoglutamic peptidase, scp-b, scytalidopepsin b, scytalido-carboxyl peptidase-b, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Scytalido-carboxyl peptidase-B
-
Ganoderma lucidum carboxyl proteinase
-
-
-
-
Proteinase, Ganoderma lucidum aspartic
-
-
-
-
Proteinase, Scytalidium lignicolum aspartic, B
-
-
-
-
Scytalidium aspartic proteinase B
-
-
-
-
Scytalidium lignicolum pepstatin-insensitive carboxyl peptidase
-
-
scytalidoglutamic peptidase
-
-
SLB
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins with broad specificity, cleaving Phe24-/-Phe, but not Leu15-Tyr and Phe25-Tyr in the B chain of insulin
show the reaction diagram
CAS REGISTRY NUMBER
COMMENTARY hide
104781-89-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLA + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLA + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLD + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLD + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLEPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLE + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLES + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLE + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLF + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLF + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLG + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLG + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLH + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLH + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLK + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLK + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLLPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLL + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLL + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLMPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLM + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLM + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLN + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLN + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLPPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLP + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLP + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLQ + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLQ + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLR + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLR + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLS + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLS + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLVPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLV + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLV + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLY + PSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine] + H2O
2-aminobenzoyl-KLY + SSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
show the reaction diagram
-
-
-
-
?
angiotensin I + H2O
?
show the reaction diagram
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O
?
show the reaction diagram
-
preferentially cleaved at His-Pro and His-Leu, less rapidly at Tyr-Ile
-
-
?
Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala + H2O
Benzyloxycarbonyl-Ala-Ala-Lys + Ala-Ala-Ala
show the reaction diagram
-
slow hydrolysis
-
?
Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala + H2O
Benzyloxycarbonyl-Ala-Lys + Ala-Ala-Ala
show the reaction diagram
-
slow hydrolysis
-
?
Benzyloxycarbonyl-Lys-Leu-Ala-Ala + H2O
Benzyloxycarbonyl-Lys-Leu-Ala + Ala
show the reaction diagram
-
slow hydrolysis
-
?
Benzyloxycarbonyl-Phe-Leu-Ala-Ala + H2O
Benzyloxycarbonyl-Phe-Leu-Ala + Ala
show the reaction diagram
-
slow hydrolysis
-
?
Benzyloxycarbonyl-Phe-Tyr-Ala-Ala + H2O
Benzyloxycarbonyl-Phe-Tyr-Ala + Ala
show the reaction diagram
-
slow hydrolysis
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
-
-
-
-
?
D-2,3-diaminopropionic acid(N-methylanthraniloyl)-GFKFFALRK(2,4-dinitrophenol) + H2O
D-2,3-diaminopropionic acid(N-methylanthraniloyl)-GFKFF + ALRK(2,4-dinitrophenol)
show the reaction diagram
-
-
-
-
?
FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O
FVNQHLCGSHLVE + A + LYLVCGERGF + FY + TPKA
show the reaction diagram
-
i.e. insulin B chain, cleavage site specificity
-
-
?
Oxidized insulin B-chain + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
-
specifically hydrolyzes the Tyr26-Tyr27 bond, also hydrolyzes Glu13-Ala14, Ala14-Leu15, Phe24-Phe25, and less rapidly Tyr16-Leu17 and Glu21-Arg22
-
-
?
Proangiotensin + H2O
?
show the reaction diagram
-
rapid hydrolysis of His6-Pro7 bond, additional cleavage of the Tyr4-Ile5 bond
-
-
?
Z-Phe-Xaa-Ala-Ala + H2O
Z-Phe + Xaa-Ala-Ala
show the reaction diagram
-
C-terminal cleavage site specificity, low activity
-
-
?
Z-Xaa-Leu-Ala-Ala + H2O
Z-Xaa + Leu-Ala-Ala
show the reaction diagram
-
C-terminal cleavage site specificity, low activity
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme is involved in protein degradation in wood destroying by the fungus
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CuCl2
-
10 mM, activates
MnCl2
-
10 mM, activates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-epoxy-3-(4-nitrophenoxy)propane
1-diazo-3-phenyl-2-propanone
-
modifies active site residue Asp98
Ac-FKF-(3S,4S)-phenylstatinyl-LR-NH
-
-
acetyl-Phe--Lys-Phe-(3S,4S)-phenylstatinyl-Leu-Arg-NH2
-
SGP transition state mimic inhibitor
N-bromosuccinimide
-
-
NaCl
-
a substantial decrease of the SGP catalytic efficiency (kcat/Km) is observed as the NaCl concentration is increases up to 1 M, while in the range up to 200 mM NaCl the effect is mainly in Km because there is no significant decrease in kcat values. A significant decrease in the kcat values is observed as the salt concentration is further increased from 200 to 800 mM NaCl
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0022
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0023
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0039
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0046
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.004
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0008
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0002
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0012
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.002
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0046
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0001
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0004
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.00055
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0005
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0016
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0008
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.00026
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.003
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0004
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0009
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0008
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0003
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0008
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0014
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0004
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0017
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.00025
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.0011
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.000065
D-2,3-diaminopropionic acid(N-methylanthraniloyl)-GFKFFALRK(2,4-dinitrophenol)
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.6
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
6
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.6
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
17
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.4
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.2
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
13
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
200
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.4
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
3.6
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
33
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
145
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
3.5
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
8
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
7.7
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
105
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
6.5
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
198
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
7.7
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.4
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.8
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
1.1
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
15
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.03
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.063
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
0.048
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
8.7
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
86
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
34.8
D-2,3-diaminopropionic acid(N-methylanthraniloyl)-GFKFFALRK(2,4-dinitrophenol)
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
700
2-aminobenzoyl-KLAPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
2600
2-aminobenzoyl-KLASSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
150
2-aminobenzoyl-KLDPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
3700
2-aminobenzoyl-KLDSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
100
2-aminobenzoyl-KLESPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
250
2-aminobenzoyl-KLESSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
65000
2-aminobenzoyl-KLFPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
167000
2-aminobenzoyl-KLFSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
200
2-aminobenzoyl-KLGPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
800
2-aminobenzoyl-KLGSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
330000
2-aminobenzoyl-KLHPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
363000
2-aminobenzoyl-KLHSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
6400
2-aminobenzoyl-KLKPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
16000
2-aminobenzoyl-KLKSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
4800
2-aminobenzoyl-KLLSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
131000
2-aminobenzoyl-KLMSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
26000
2-aminobenzoyl-KLNPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
66000
2-aminobenzoyl-KLNSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
19000
2-aminobenzoyl-KLPSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
400
2-aminobenzoyl-KLQPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
1000
2-aminobenzoyl-KLQSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
3700
2-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
19000
2-aminobenzoyl-KLRSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
20
2-aminobenzoyl-KLSPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
160
2-aminobenzoyl-KLSSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
30
2-aminobenzoyl-KLVSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
35000
2-aminobenzoyl-KLYPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
78000
2-aminobenzoyl-KLYSSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
in 100 mM sodium acetate buffer (pH 4.5) at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
-
substrate casein
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.3 - 3.5
-
1.3: about 80% of activity maximum, 3.5: about 35% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 68
-
37°C: about 30% of activity maximum, 68°C: about 50% of activity maximum
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
the enzyme is secreted
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PRTB_SCYLI
260
0
27165
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21969
-
1 * 21969, amino acid sequence calculation
21970
-
Scytalidium lignicolum, calculation from amino acid sequence
22000
-
Scytalidium lignicolum, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 21969, amino acid sequence calculation
additional information
-
molecular structure analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no glycoprotein
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme-(acetyl-FKF-(3S,4S)-phenylstatinyl)-LR-NH2 complex and enzyme-acetyl-FKF(2R,3S)-phenylisoseryl-ALR-NH2 complex. Native crystals of the enzyme are grown at room temperature by the hanging-drop vapor diffusion method from 42% saturated ammonium sulfate, 10% ethylene glycol and 0.1 M sodium acetate buffer (pH 4.0). These crystals are then soaked in solutions of varying concentrations of the transition state analogs (acetyl-FKF-(3S,4S)-phenylstatinyl)-LR-NH2 and acetyl-FKF(2R,3S)-phenylisoseryl-ALR-NH2
-
purified enzyme complexed with angiotensin II, hanging drop method, room temperature, from 42% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.0, 10% v/v ethylene glycol, crystals are soaked in cryosolution containing 30% glvcerol, 45% saturated ammonium sulfate,and 0.1 M sodium acetate, pH 4.0, heavy atom derivatizing, X-ray diffraction structure determination and analysis at 1.9-2.5 A resolution, molecular modeling and multiple isomorphous replacement phasing
-
the three-dimensional structure of SGP is determined by X-ray crystallography. The polypeptide folds into a beta-sandwich that has seven antiparallel beta-strands in each of the two sheets
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D43A
the mutant has approximately 20% of the original activity
E136A
-
mutant loses the ability for self-activation and cleavage of the propeptide. Mutant has no peptidase activity
Q53A
-
mutant loses the ability for self-activation and cleavage of the propeptide. Mutant has no peptidase activity
Q53E
-
mutant loses the ability for self-activation and cleavage of the propeptide. Mutant has no peptidase activity
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5 - 5
-
37°C, 20 h
30754
8 - 9.5
-
in the pH range 8.0-9.5, the enzyme becomes irreversibly inactivated
709118
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
pH 1.5-5.5, 20 h, stable
60
-
pH 4.0, 15 min, stable below
68
-
pH 4.0, 15 min, complete loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae with propper processing prior to secretion
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Majima, E.; Oda, K.; Murao, S.; Ichishima, E.
Comparative study on the specificities of several fungal aspartic and acidic proteinases towards the tetradecapeptide of a renin substrate
Agric. Biol. Chem.
52
787-793
1988
Scytalidium lignicola
-
Manually annotated by BRENDA team
Oda, K.; Murao, S.
New acid proteases from Scytalidium lignicolum M-133. II. Purification and some enzymatic properties of acid protease A and B of Scytalidium lignicolum ATCC 24568.
Agric. Biol. Chem.
38
2435-2444
1974
Scytalidium lignicola
-
Manually annotated by BRENDA team
Oda, K.; Murao, S.
Studies on new acid proteases from Scytalidium lignicolum M-133. Part V. Action of Scytalidium lignicolum acid proteases on the insulin B-chain
Agric. Biol. Chem.
40
1221-1225
1976
Scytalidium lignicola
-
Manually annotated by BRENDA team
Ichishima, E.; Majima, E.; Emi, M.; Hayashi, K.; Murao, S.
Enzymic cleavage of the histidyl-prolyl bond of proangiotensin by carboxyl proteinases from Aspergillus sojae and Scytalidium lignicolum
Agric. Biol. Chem.
45
2391-2393
1981
Scytalidium lignicola
-
Manually annotated by BRENDA team
Morihara, K.; Tsuzuki, H.; Murao, S.; Oda, K.
Pepstatin-insenstive acid proteases from Scytalidium lignicolum. Kinetic study with synthetic peptides
J. Biochem.
85
661-668
1979
Scytalidium lignicola
Manually annotated by BRENDA team
Oda, K.; Murao, S.
Studies on new acid proteases from Scytalidium lignicolum ATCC 24568. Part IX. Additional evidence for the identity of Scytalidium lignicolum acid proteinases with the carboxyl proteinase group: the interaction between angiotensin I and S-PI-insensitive acid proteinases by means of a zinc(II)-dye complex as a probe
Agric. Biol. Chem.
50
1995-2001
1986
Scytalidium lignicola
-
Manually annotated by BRENDA team
Maita, T.; Nagata, S.; Matsuda, G.; Maruta, S.; Oda, K.; Murao, S.; Tsuru, D.
Complete amino acid sequence of Scytalidium lignicolum acid protease B
J. Biochem.
95
465-475
1984
Scytalidium lignicola
Manually annotated by BRENDA team
Tsuru, D.; Shimada, S.; Maruta, S.; Yoshimoto, T.; Oda, K.; Murao, S.; Miyata, T.; Iwanaga, S.
Isolation and amino acid sequence of a peptide containing an epoxide-reactive residue from the thermolysin-digest of Scytalidium lignicolum acid protease B
J. Biochem.
99
1537-1539
1986
Scytalidium lignicola
Manually annotated by BRENDA team
Oda, K.
Scytalidopepsin B
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
219-221
2004
Scytalidium lignicola
-
Manually annotated by BRENDA team
Fujinaga, M.; Cherney, M.M.; Oyama, H.; Oda, K.; James, M.N.
The molecular structure and catalytic mechanism of a novel carboxyl peptidase from Scytalidium lignicolum
Proc. Natl. Acad. Sci. USA
101
3364-3369
2004
Scytalidium lignicola
Manually annotated by BRENDA team
James, M.N.
The peptidases from fungi and viruses
Biol. Chem.
387
1023-1029
2006
Scytalidium lignicola
Manually annotated by BRENDA team
Pillai, B.; Cherney, M.M.; Hiraga, K.; Takada, K.; Oda, K.; James, M.N.
Crystal structure of Scytalidoglutamic peptidase with its first potent inhibitor provides insights into substrate specificity and catalysis
J. Mol. Biol.
365
343-361
2007
Scytalidium lignicola
Manually annotated by BRENDA team
Kondo, M.Y.; Okamoto, D.N.; Santos, J.A.; Juliano, M.A.; Oda, K.; Pillai, B.; James, M.N.; Juliano, L.; Gouvea, I.E.
Studies on the catalytic mechanism of a glutamic peptidase
J. Biol. Chem.
285
21437-21445
2010
Scytalidium lignicola
Manually annotated by BRENDA team
Stocchi, N.; Revuelta, M.; Castronuovo, P.; Vera, D.; ten Have, A.
Molecular dynamics and structure function analysis show that substrate binding and specificity are major forces in the functional diversification of eqolisins
BMC Bioinformatics
19
338
2018
Scytalidium lignicola (P15369)
Manually annotated by BRENDA team