native isozymes Ia and Ib 30fold to homogeneity from mycel-free culture filtrate, by acetone precipitation, anion exchange chromatograph, ammonium sulfate fractionation, dialysis and crystallization to homogeneity, and further to puritiy by anion exchange and sulfopropyl chromatograpgy, and isoelectric focusing
Kumagai, H.; Matsue, M.; Majima, E.; Tomoda, K.; Ichishima, E.
Carboxyl proteinase from the wood deteriorating basidiomycete Pycnoporus coccineus: substrate specificity with oxidized insulin peptide B1 .apprx. B16 and B15 .apprx. B24, angiotensin and proangiotensin