Information on EC 3.4.23.30 - Pycnoporopepsin

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The expected taxonomic range for this enzyme is: Trametes

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EC NUMBER
COMMENTARY hide
3.4.23.30
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RECOMMENDED NAME
GeneOntology No.
Pycnoporopepsin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Similar to pepsin A, but narrower, cleaving only three bonds in the B chain of insulin: Ala14-/-Leu, Tyr16-/-Leu, and Phe24-/-Phe
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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aspartic endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
77967-78-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
a wood-deteriorating basidiomycete, formerly Trametes sanguinea, isozymes Ia and Ib
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Manually annotated by BRENDA team
i.e. Pycnoporus coccineus, Trametes sanguinea
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Angiotensin + H2O
?
show the reaction diagram
Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O
Asp-Arg-Val-Tyr + Ile-His-Pro-Phe-His-Leu-Leu + Val-Tyr-Ser
show the reaction diagram
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cleavage of Tyr-Ile and Leu-Val
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casein + H2O
?
show the reaction diagram
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?
FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O
FVNQHLCGSHLVEA + Leu-Val + LVCGERGF + FYTPKA
show the reaction diagram
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i.e. oxidized insulin B chain, cleavage site specificity of isozymes Ia and Ib at pH 2.7, overview
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?
Hemoglobin + H2O
?
show the reaction diagram
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?
Native insulin + H2O
Hydrolyzed insulin
show the reaction diagram
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Ala14-Leu15, Tyr16-Leu17 and Phe24-Phe25 bonds are hydrolyzed
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Oxidized B-chain of insulin + H2O
Hydrolyzed insulin B-chain
show the reaction diagram
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Ala14-Leu15, Tyr16-Leu17 and Phe24-Phe25 bonds are hydrolyzed primarily
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Oxidized insulin peptide B1-B16 + H2O
?
show the reaction diagram
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hydrolysis at His10-Leu11 and Ala14-Leu15
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pro-angiotensin + H2O
?
show the reaction diagram
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cleavage at Tyr4-Ile5 and Phe8-Phe9, at pH 2.7
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?
Proangiotensin + H2O
?
show the reaction diagram
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formerly designated angiotensin I, hydrolysis of Tyr4-Ile5
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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the extracellular enzyme is involved in fungal nutrition from wood
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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purified isozyme Ia and Ib
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.3
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substrate hemoglobin
2.5
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substrate casein
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
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isozyme Ib
4.7
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isozyme Ia
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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crude enzyme preparation
Manually annotated by BRENDA team
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submerged culture
Manually annotated by BRENDA team
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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the isozymes are secreted
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Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000 - 34000
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sedimentation equilibrium centrifugation analysis
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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three-dimensional structure and active site structure
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified isozymes Ia and Ib from acetone
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
i.e. Pycnoporus coccineus, Trametes sanguinea
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native isozymes Ia and Ib 30fold to homogeneity from mycel-free culture filtrate, by acetone precipitation, anion exchange chromatograph, ammonium sulfate fractionation, dialysis and crystallization to homogeneity, and further to puritiy by anion exchange and sulfopropyl chromatograpgy, and isoelectric focusing
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LINKS TO OTHER DATABASES (specific for EC-Number 3.4.23.30)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)