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Information on EC 3.4.23.29 - Polyporopepsin

for references in articles please use BRENDA:EC3.4.23.29

Word Map on EC 3.4.23.29

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The expected taxonomic range for this enzyme is: Irpex lacteus

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3.4.23.29

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Polyporopepsin

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Milk clotting activity, broad specificity, but fails to cleave Leu15-Tyr or Tyr16-Leu of insulin B chain

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hydrolysis of peptide bond

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aspartic endopeptidase

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Show Synonyms (9 entries)

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61573-73-7

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Ala-Phe-Gly-Ala + H2O

Ala-Phe + Gly-Ala

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Ala-Phe-Leu-Ala + H2O

Ala-Phe + Leu-Ala

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alpha1-casein + H2O

?

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cleavage of Phe23-Phe24 and Lys103-Tyr104 bonds at pH 6.0

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Angiotensin I + H2O

Proteolytically cleaved angiotensin

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hydrolyzes Tyr4-Ile5 bond much more rapidly than the Val3-Tyr4 bond

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beta-casein + H2O

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show the reaction diagram

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cleavage of Leu165-Ser166, Ala189-Phe190, and Leu192-Tyr193 bonds, no cleavage of Leu139-Leu140 and Ser142-Trp143 bonds

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FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O

FVNQHLCGSHL + VEA + LYLVCGERGF + FYT + PKA

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i.e. insulin B chain, cleavage site specificity at pH 3.0.the Ala14-Leu15 bond is preferred

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?

Gly-Phe-Leu-Ala + H2O

Gly-Phe + Leu-Ala

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Hemoglobin + H2O

?

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?

Hemoglobin + H2O

Proteolytically cleaved hemogobin

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kappa-casein + H2O

?

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cleavage of Phe105-Met106 bond

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Oxidized B-chain of insulin + H2O

Proteolytically cleaved insulin B-chain

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peptide bonds mainly susceptible to the enzyme: Leu11-Val12, Ala14-Leu15, Phe24-Phe25, Thr27-Pro28

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Phe-Ala-Ala-Ala + H2O

Phe-Ala + Ala-Ala

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Phe-Glu-Ala-Ala + H2O

Phe-Glu + Ala-Ala

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Phe-Gly-Ala-Ala + H2O

Phe-Gly + Ala-Ala

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Phe-Leu-Ala-Ala + H2O

Phe-Leu + Ala-Ala

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Phe-Lys-Ala-Ala + H2O

Phe-Lys + Ala-Ala

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Phe-Tyr-Ala-Ala + H2O

Phe-Tyr + Ala-Ala

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additional information

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1,2-epoxy-3-(4-nitrophenoxy)propane

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chymostatin

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Diazoacetyl-D,L-norleucine methyl ester

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Diazoacetyl-DL-norleucine methyl ester

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inactivation, active site-directed inhibitor

Fe3+

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Hg2+

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N-p-toluene sulfonyl-L-Phe chloromethyl ketone

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Pepstatin

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sodium lauryl sulfate

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additional information

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2.5

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2.8

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hemoglobin

2.8 - 2.9

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substrates casein and hemoglobin

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hemoglobin

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Phe-Leu-Ala-Ala

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5.3

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1wkr, download, 3D-view

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32000

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Polyporus tulipiferae, 3U-39, gel filtration

33500

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Polyporus tulipiferae 2U-148, gel filtration

34000

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Polyporus tulipiferae KY 2901 and U-212, gel filtration

35000

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Polyporus tulipiferae, calculation from nucleotide sequence

36000

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39000

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1 * 39000, Polyporus tulipiferae, SDS-PAGE

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?

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x * 36000, SDS-PAGE

monomer

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1 * 39000, Polyporus tulipiferae, SDS-PAGE

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glycoprotein

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two possible N-glycosylation sites at Asn192 and Asn228, the enzyme shows affinity for concanavalin A, wheat germ agglutinin, and Ricinus communis agglutinin

additional information

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2 putative N-glycosylation sites, sugar chains can be attached to Asn192 and/or Asn238

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crystallization by hanging drop method with ammonium sulfate as precipitant, X-ray diffraction structure determination and analysis at 1.9 A resolution

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enzyme from Fr. KY 2901 and mutant enzymes produced by irradiation with UV-light

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purified enzyme in complex with inhibitor pepstatin, hanging drop vapour diffusion method, 0.005 ml of 10 mg/ml protein is mixed with 0.005 ml of reservoir solution containing 50% ammonium sulfate in 10 mM sodium citrate-sulfate buffer, pH 5.4, 20°C, X-ray diffraction structure determination and analysis at 1.3 A resolution, structure modeling

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3 - 6

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stable

30731

4.6

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15 min, complete inactivation at 45°C, stable at 30°C

30730

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30

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pH 4.6, 15 min, stable

45

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pH 4.6, 15 min, complete inactivation

50

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pH 4.5, 15 min, stable up to

60

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pH 4.5, 15 min, complete inactivation

additional information

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the enzyme is the least heat-stable among the milk-clotting enzymes

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30728, 30730, 30731

native enzyme by dehydroacetylpepstatin affinity chromatography

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DNA and amino acid sequence determination and analysis

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Show AA Sequence (4 entries)

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Show References (6 entries)

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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)