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Information on EC 3.4.22.B70 - SENP1 peptidase and Organism(s) Mus musculus and UniProt Accession P59110

for references in articles please use BRENDA:EC3.4.22.B70
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B70 SENP1 peptidase
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Mus musculus
UNIPROT: P59110 not found.
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
The enzyme catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO-1, SUMO-2 and SUMO-3 to their mature forms and deconjugation of SUMO-1, SUMO-2 and SUMO-3 from targeted proteins. Deconjugates SUMO-1 from homeodomain-interacting protein kinase 2. Deconjugates SUMO-1 from histone deacetylase 1, which decreases its transcriptional repression activity. Cleavage of Gly97-/-His98 bond in the SUMO-1 precursor with release of the propeptide His-Ser-Thr-Val. Cleavage of Gly93-/-Val94 bond in the SUMO-2 precursor with release of the propeptide Val94-Thyr. Cleavage of the Gly92-/-Val93 in the SUMO-3 precursor with release of the propeptide Pro-Glu-Ser-Ser-Leu-Ala-Gly-His-Ser-Phe.
Synonyms
sumo isopeptidase, sumo-specific protease 1, uspl1, sentrin/sumo-specific protease 1, sumo-specific isopeptidase, sentrin-specific protease 1, ubiquitin-specific protease-like 1, small ubiquitin-like modifier protein-specific protease 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SENP1
-
-
SYSTEMATIC NAME
IUBMB Comments
Q9P0U3 {SwissProt}
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
SUMO-GATA 1 conjugate + H2O
SUMO + GATA1
show the reaction diagram
SUMO-hypoxia-inducible factor 1 conjugate + H2O
SUMO + hypoxia-inducible factor 1
show the reaction diagram
SUMOylated CD45 + H2O
?
show the reaction diagram
SENP1 can deconjugate SUMOylated CD45
-
-
?
SUMO1ylated protein + H2O
SUMO1 + protein
show the reaction diagram
SUMO2ylated protein + H2O
SUMO2 + protein
show the reaction diagram
-
-
-
-
?
SUMO3ylated protein + H2O
SUMO3 + protein
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
recombinant enzyme Senp1 can deconjugate terminal Sumo1 moieties on poly-Sumo2/3 chains
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
SUMO-GATA 1 conjugate + H2O
SUMO + GATA1
show the reaction diagram
SENP1 promotes GATA1 activation and subsequent erythropoiesis by deSUMOylating GATA1
-
-
?
SUMO-hypoxia-inducible factor 1 conjugate + H2O
SUMO + hypoxia-inducible factor 1
show the reaction diagram
deSUMOylation plays a critical role in prostate pathogenesis through induction of HIF1alpha-dependent angiogenesis and enhanced cell proliferation
-
-
?
SUMO1ylated protein + H2O
SUMO1 + protein
show the reaction diagram
-
main substrate
-
-
?
SUMO2ylated protein + H2O
SUMO2 + protein
show the reaction diagram
-
-
-
-
?
SUMO3ylated protein + H2O
SUMO3 + protein
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetamide
-
inhibits desumoylation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SENP1 modulates prostate epithelial cell proliferation
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
malfunction
-
embryos homozygous for this mutant allele, Senp1M1Mku show phenotypic abnormalities beginning at embryonic day (E) 11.5 with the majority dying between E12.5 and E14.5. Enzyme defects lead to accumulation of Sumo1-modified proteins in homozygous embryos, a lethal process, phenotypes, overview. Senp1Gt mutants express a truncated Senp1 affecting Sumo2/3 levels
physiological function
-
enzyme Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo, overview. Senp1 is the primary activity for desumoylation of Sumo1-modified proteins
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SENP1_MOUSE
640
0
72511
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C599S
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site-directed mutagenesis, the active site mutant precipitates SUMO1ylated proteins but not SUMO2/3-modified substrate
additional information
-
construction of several allelic defective enzyme mutants and a truncated mutant Senp1, overview, and a Senp1-beta-geo fusion protein, Expression of the amino half of Senp1 reduces Senp2 levels
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
SENP1, recombinant expression of wild-type and mutant enzymes
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
SENP1 overexpression induces transformation of the normal prostate gland and gradually facilitates the onset of high-grade prostatic intraepithelial neoplasiade. SUMOylation plays a critical role in prostate pathogenesis through induction of HIF1alpha-dependent angiogenesis and enhanced cell proliferation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cheng, J.; Kang, X.; Zhang, S.; Yeh, E.T.
SUMO-specific protease 1 is essential for stabilization of HIF1alpha during hypoxia
Cell
131
584-595
2007
Mus musculus (P59110)
Manually annotated by BRENDA team
Dai, X.Q.; Plummer, G.; Casimir, M.; Kang, Y.; Hajmrle, C.; Gaisano, H.Y.; Manning Fox, J.E.; MacDonald, P.E.
SUMOylation regulates insulin exocytosis downstream of secretory granule docking in rodents and humans
Diabetes
60
838-847
2011
Mus musculus (P59110)
Manually annotated by BRENDA team
Bawa-Khalfe, T.; Cheng, J.; Lin, S.H.; Ittmann, M.M.; Yeh, E.T.
SENP1 induces prostatic intraepithelial neoplasia through multiple mechanisms
J. Biol. Chem.
285
25859-25866
2010
Mus musculus (P59110)
Manually annotated by BRENDA team
Yu, L.; Ji, W.; Zhang, H.; Renda, M.J.; He, Y.; Lin, S.; Cheng, E.C.; Chen, H.; Krause, D.S.; Min, W.
SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis
J. Exp. Med.
207
1183-1195
2010
Mus musculus (P59110)
Manually annotated by BRENDA team
Sharma, P.; Yamada, S.; Lualdi, M.; Dasso, M.; Kuehn, M.R.
Senp1 is essential for desumoylating Sumo1-modified proteins but dispensable for Sumo2 and Sumo3 deconjugation in the mouse embryo
Cell Rep.
3
1640-1650
2013
Mus musculus
Manually annotated by BRENDA team
Huang, X.; Zuo, Y.; Wang, X.; Wu, X.; Tan, H.; Fan, Q.; Dong, B.; Xue, W.; Chen, G.Q.; Cheng, J.
SUMO-specific protease 1 is critical for myeloid-derived suppressor cell development and function
Cancer Res.
79
3891-3902
2019
Mus musculus (P59110)
Manually annotated by BRENDA team
Zhang, H.; Wang, Y.; Zhu, A.; Huang, D.; Deng, S.; Cheng, J.; Zhu, M.X.; Li, Y.
SUMO-specific protease 1 protects neurons from apoptotic death during transient brain ischemia/reperfusion
Cell Death Dis.
7
e2484
2016
Mus musculus (P59110)
Manually annotated by BRENDA team