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Information on EC 3.4.22.B65 - cathepsin P and Organism(s) Mus musculus and UniProt Accession Q9R014

for references in articles please use BRENDA:EC3.4.22.B65
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B65 cathepsin P
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This record set is specific for:
Mus musculus
UNIPROT: Q9R014
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Hydrolysis of proteins with restricted preference for substrates containing hydrophobic amino acids. Efficiently cleaves -Glu-Ile-Phe-/-Val-Phe-Lys-Gln-. Involved in the processing of endoplasmic proteins
Synonyms
cathepsin p, more
CAS REGISTRY NUMBER
COMMENTARY hide
94716-08-02
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 + H2O
7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly + Leu-Dap(Dnp)-Ala-Arg-NH2
show the reaction diagram
azocasein + H2O
?
show the reaction diagram
-
-
-
?
calreticulin + H2O
?
show the reaction diagram
endoplasmic reticulum protein substrate identified by incubation of recombinant enzyme with rat choriocarcinoma cell proteins
protein is processed from the C-terminus, with removal of the KDEL endoplasmic reticulum retention signal. Cathepsin L co-localizes with calreticulin in rat choriocarcinoma cell
-
?
Gp96 protein + H2O
?
show the reaction diagram
endoplasmic reticulum protein substrate identified by incubation of recombinant enzyme with rat choriocarcinoma cell proteins
protein is sequentially processed by cathepsin P at 2 sites towards the C-terminus of the protein, with removal of the KDEL endoplasmic reticulum retention signal. Cathepsin L co-localizes with gp96 in rat choriocarcinoma cell
-
?
o-aminobenzoic acid-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-ALR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-EIFVFKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-EIF + VFKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
ideal substrate constructed after analysis of substrate preferences. In contrast to most substrates, hydrolysis is not stimulated by addition of salts
-
-
?
o-aminobenzoic acid-ELRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-ELR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-FLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-FLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-HLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-HLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-ILRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-ILR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KARSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KAR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KFRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KFR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KIRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KIR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLASSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLA + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLFSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLF + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLISSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLI + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine + o-aminobenzoic acid-KLIS + SKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
preferential hydrolysis at the Ser-Ser bond
-
?
o-aminobenzoic acid-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLK + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLLSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLL + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLMSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLM + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLQSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLQ + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRESKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + ESKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRfSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + fSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRGSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + GSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRHSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + HSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRISKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + ISKQ-N-(2,4-dinitrophenyl)ethylenediamine + o-aminobenzoic acid-KLRIS + KQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
30% cleavage at Arg-Ile bond, 70% cleavage at Ser-Lys bond
-
?
o-aminobenzoic acid-KLRLSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + LSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRNSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + NSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRQSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + QSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRRSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + RSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLR + VSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLTSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLT + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KLYSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KLY + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KMRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KMR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KNLRSSKQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
?
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KQRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KQR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KRRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KRR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KTRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KTR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KVRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KVR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-KYRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-KYR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-LLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-LLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-NLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-NLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-RLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-RLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
o-aminobenzoic acid-VLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
o-aminobenzoic acid-VLR + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
show the reaction diagram
-
-
-
?
transferrin + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
biotin-(6-hexanamido-hexanoate)2-Tyr-Ala-CHN2
-
CBZ-Trp-Trp-4-cyclohexanone-Trp-Phe-OMe
reversoble competitive inhibition. 10fold less inhibitory on mouse cathepsin L, 20fold less inhibitory on human cathpsin L, 50fold less inhibotry on human cathepsin B
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
activation
hyaluronate
-
Na2SO4
NaBr
1-2.5 M, up to 5fold increase in activity
NaCl
1-2.5 M, up to 5fold increase in activity
NaH2PO4
1-2.5 M, up to 5fold increase in activity
Sodium acetate
1-2.5 M, up to 5fold increase in activity
Sodium citrate
1-2.5 M, up to 5fold increase in activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
o-aminobenzoic acid-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.15 - 0.5
o-aminobenzoic acid-EIFVFKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0051
o-aminobenzoic acid-ELRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0094
o-aminobenzoic acid-FLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0048
o-aminobenzoic acid-HLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0085
o-aminobenzoic acid-ILRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0184
o-aminobenzoic acid-KARSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.004
o-aminobenzoic acid-KFRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0069
o-aminobenzoic acid-KIRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0034
o-aminobenzoic acid-KLASSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0015
o-aminobenzoic acid-KLFSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0015
o-aminobenzoic acid-KLLSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0014
o-aminobenzoic acid-KLMSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0029 - 0.013
o-aminobenzoic acid-KLQSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0053
o-aminobenzoic acid-KLRESKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0079
o-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0058
o-aminobenzoic acid-KLRGSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0153
o-aminobenzoic acid-KLRHSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0046
o-aminobenzoic acid-KLRLSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0202
o-aminobenzoic acid-KLRNSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0084
o-aminobenzoic acid-KLRQSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0107
o-aminobenzoic acid-KLRRSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.004 - 0.01
o-aminobenzoic acid-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.0014
o-aminobenzoic acid-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.002
o-aminobenzoic acid-KLTSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0038
o-aminobenzoic acid-KLYSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0084
o-aminobenzoic acid-KMRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0048
o-aminobenzoic acid-KNLRSSKQ-(N-(2,4-dinitrophenyl)ethylenediamine)
37°C, pH 6.5
0.0201
o-aminobenzoic acid-KQRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.018
o-aminobenzoic acid-KRRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0243
o-aminobenzoic acid-KTRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0055
o-aminobenzoic acid-KVRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.00044
o-aminobenzoic acid-KYRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.018
o-aminobenzoic acid-LLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0086
o-aminobenzoic acid-NLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.013
o-aminobenzoic acid-RLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.004
o-aminobenzoic acid-VLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
o-aminobenzoic acid-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.004 - 0.006
o-aminobenzoic acid-EIFVFKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.021
o-aminobenzoic acid-ELRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.024
o-aminobenzoic acid-FLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.013
o-aminobenzoic acid-HLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.016
o-aminobenzoic acid-ILRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.008
o-aminobenzoic acid-KARSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.004
o-aminobenzoic acid-KFRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.05
o-aminobenzoic acid-KIRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.009
o-aminobenzoic acid-KLASSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.016
o-aminobenzoic acid-KLFSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.02
o-aminobenzoic acid-KLKSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.012
o-aminobenzoic acid-KLLSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.013
o-aminobenzoic acid-KLMSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.006
o-aminobenzoic acid-KLQSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.014
o-aminobenzoic acid-KLRESKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.01
o-aminobenzoic acid-KLRFSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.007
o-aminobenzoic acid-KLRGSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.009
o-aminobenzoic acid-KLRHSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.018
o-aminobenzoic acid-KLRLSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.044
o-aminobenzoic acid-KLRNSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.029
o-aminobenzoic acid-KLRQSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.018
o-aminobenzoic acid-KLRRSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.0014 - 0.016
o-aminobenzoic acid-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
0.009
o-aminobenzoic acid-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.007
o-aminobenzoic acid-KLTSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.015
o-aminobenzoic acid-KLYSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.02
o-aminobenzoic acid-KMRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.016
o-aminobenzoic acid-KNLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.006
o-aminobenzoic acid-KQRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.001
o-aminobenzoic acid-KRRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.013
o-aminobenzoic acid-KTRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.017
o-aminobenzoic acid-KVRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.005
o-aminobenzoic acid-KYRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.029
o-aminobenzoic acid-LLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.012
o-aminobenzoic acid-NLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.023
o-aminobenzoic acid-RLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
0.013
o-aminobenzoic acid-VLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
37°C, pH 6.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000032
CBZ-Trp-Trp-4-cyclohexanone-Trp-Phe-OMe
pH 6.5, 37°C
0.002
E-64
37°C, presence of 1.5 M NaSO4
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
substrates azocasein and transferrin
7
substrate 7-(methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-Dnp(Dpa)-Ala-Arg-NH2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
little activity below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
increased expression of cathepsin P late in gestation
Manually annotated by BRENDA team
low level of expression in visceral yolk sac
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CATJ_MOUSE
334
0
37276
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
27000
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
31000
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
35000
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
38000
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 31000, x * 35000, x * 38000, SDS-PAGE of recombinant zymogen, x * 24000, x * 27000, SDS-PAGE of mature protein
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
treatment of recombinant protein with PNGase F results in a single protein band of 29000 Da in SDS-PAGE for the zymogen and of 22000 Da for the autoactivated protein
proteolytic modification
the inactive zymogen can be activated by proteinase K, chymotrypsin or pancreatic elastase at neutral pH. Cathepsin P can be autoactivated at neutral pH, but not at acidic pH
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimethyl sulfoxide
increased concentration of dimethyl sulfoxide reduces Vmax but does not affect Km for substrate MOCAc-Pro-Leu-Gly-Leu-A2pr(Dnp)-Ala-Arg-NH2
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Puzer, L.; Barros, N.M.; Oliveira, V.; Juliano, M.A.; Lu, G.; Hassanein, M.; Juliano, L.; Mason, R.W.; Carmona, A.K.
Defining the substrate specificity of mouse cathepsin P
Arch. Biochem. Biophys.
435
190-196
2005
Mus musculus (Q9R014), Mus musculus
Manually annotated by BRENDA team
Hassanein, M.; Xue, F.; Seto, C.T.; Mason, R.W.
Development of a specific inhibitor for the placental protease, cathepsin P
Arch. Biochem. Biophys.
464
288-294
2007
Mus musculus (Q9R014), Mus musculus
Manually annotated by BRENDA team
Sol-Church, K.; Frenck, J.; Mason, R.W.
Cathepsin Q, a novel lysosomal cysteine protease highly expressed in placenta
Biochem. Biophys. Res. Commun.
267
791-795
2000
Mus musculus (Q9R014), Mus musculus
Manually annotated by BRENDA team
Mason, R.W.; Bergman, C.A.; Lu, G.; Frenck Holbrook, J.; Sol-Church, K.
Expression and characterization of cathepsin P
Biochem. J.
378
657-663
2004
Mus musculus (Q9R014), Mus musculus
Manually annotated by BRENDA team
Hassanein, M.; Bojja, A.S.; Glazewski, L.; Lu, G.; Mason, R.W.
Protein processing by the placental protease, cathepsin P
Mol. Hum. Reprod.
15
433-442
2009
Mus musculus (Q9R014)
Manually annotated by BRENDA team