Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.22.B6 - cathepsin B-like protease and Organism(s) Caenorhabditis elegans and UniProt Accession P43510

for references in articles please use BRENDA:EC3.4.22.B6
preliminary BRENDA-supplied EC number
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B6 cathepsin B-like protease
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Caenorhabditis elegans
UNIPROT: P43510
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Caenorhabditis elegans
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
the enzyme does not readily hyrolyze benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin (which is cleaved by cathespsin B). The enzyme is not able to cleave synthetic substrates having an Arg in position P2
Synonyms
tbcatb, catb2, cmcatb, cathepsin b-like cysteine proteinase, cathepsin b-like protease, tcocbc1, eimeripain, cathb3, trcb2, tcocbc6, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cathepsin B-like protease
-
cathepsin B-like cysteine protease
-
-
-
-
CPB
-
-
-
-
CPC protease
-
-
-
-
LmacatB
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
37353-41-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Arg-Arg-7-amido-4-methylcoumarin + H2O
Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
?
Phe-Arg-7-amido-4-methylcoumarin + H2O
Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin + H2O
Tyr-Val-Ala-Asp + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
no significant inhibitory effects for pepstatin and PMSF
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
substrates: Phe-Arg-amino methyl coumarin, Arg-Artg-amino methyl coumarin
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CPR6_CAEEL
379
0
42404
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
x * 35000, x * 45000, gelatin SDS-PAGE digestion assay
45000
x * 35000, x * 45000, gelatin SDS-PAGE digestion assay
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 35000, x * 45000, gelatin SDS-PAGE digestion assay
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged version expressed in Sf9 cell
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Miranda-Miranda, E.; Zamora-Ruiz, A.; Cossio-Bayugar, R.
Molecular cloning and expression of a Caenorhabditis elegans Cathepsin B-like Protease
Biotechnology
8
242-247
2009
Caenorhabditis elegans (P43510)
-
Manually annotated by BRENDA team