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Information on EC 3.4.22.B50 - papain-like proteinase 2 and Organism(s) Equine arteritis virus and UniProt Accession P19811

for references in articles please use BRENDA:EC3.4.22.B50
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B50 papain-like proteinase 2
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This record set is specific for:
Equine arteritis virus
UNIPROT: P19811
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Word Map
The taxonomic range for the selected organisms is: Equine arteritis virus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
responsible for the cleavages located at the N-terminus of the replicase polyprotein
Synonyms
sars-cov plpro, papain-like proteinase, papain-like cysteine proteinase, plp-2, pl2pro, ervatamin-c, erv-c, papain-like protease domain 2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mouse hepatitis virus papain-like proteinase 2
-
-
-
-
PL2-PRO
-
-
-
-
PLP-2
-
-
-
-
PLP2
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
851883-30-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
RLRGG-7-amido-4-methylcoumarin + H2O
RLRGG + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
ubiquitin + H2O
?
show the reaction diagram
-
-
-
?
ubiquitin-aminomethylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ubiquitin + H2O
?
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ub-3Br
suicide inhibitor, i.e. ubiquitin(1-75)-3-bromopropylamide
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045 - 0.155
RLRGG-7-amido-4-methylcoumarin
0.413 - 17
ubiquitin-aminomethylcoumarin
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
arteriviruses lacking PLP2 deubiquitinase activity elicit an enhanced host innate immune response
physiological function
the enzyme is essential for arterivirus replication by cleaving a site within the viral replicase polyproteins and also removes ubiquitin from cellular proteins. This deubiquitinase activity is a critical factor in arteriviral innate immune evasion
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with ubiquitin, hanging drop vapor diffusion method, using 100 mM MES, pH 6.2, 18% (w/v) polyethylene glycol 20,000
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C270A/H332A
active site mutant
I353R
the mutant exhibits about 40fold reduction in specificity toward ubiquitin compared to the wild type while the activity toward RLRGG-7-amido-4-methylcoumarin is essentially unaltered
I353W
the mutant exhibits about 10fold reduction in specificity toward ubiquitin compared to the wild type while the activity toward RLRGG-7-amido-4-methylcoumarin is essentially unaltered
T312A/I313V/I353R
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, Superdex 75 gel filtration, and Source 15Q column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-Gold (DE3) cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
vaccination of Shetland mares, either with deubiquitinase DUB-positive or DUB-negative recombinant equine arteritis virus. Upon challenge with the virulent KY84 strain of equine arteritis virus, both vaccine viruses proved to be replication competent in vivo. The DUB-negative virus provides a similar degree of protection against clinical disease as its DUB-positive parental counterpart. A possible improvement due to inactivation of PLP2 DUB activity could not be detected under these experimental conditions
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
van Kasteren, P.B.; Bailey-Elkin, B.A.; James, T.W.; Ninaber, D.K.; Beugeling, C.; Khajehpour, M.; Snijder, E.J.; Mark, B.L.; Kikkert, M.
Deubiquitinase function of arterivirus papain-like protease 2 suppresses the innate immune response in infected host cells
Proc. Natl. Acad. Sci. USA
110
E838-E847
2013
equine arteritis virus (P19811)
Manually annotated by BRENDA team
van Kasteren, P.B.; Knaap, R.C.; van den Elzen, P.; Snijder, E.J.; Balasuriya, U.B.; van den Born, E.; Kikkert, M.
In vivo assessment of equine arteritis virus vaccine improvement by disabling the deubiquitinase activity of papain-like protease 2
Vet. Microbiol.
178
132-137
2015
equine arteritis virus (P19811), equine arteritis virus Bucyrus (P19811)
Manually annotated by BRENDA team