Information on EC 3.4.22.B30 - calpain 10 and Organism(s) Homo sapiens and UniProt Accession Q9HC96

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Homo sapiens
UNIPROT: Q9HC96


The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.B30
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
calpain 10
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CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
physiological function
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the calpain-10 expression correlates positively with arginine-stimulated insulin release in islets from non-diabetic donors
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
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-
-
?
SNAP-25 + H2O
?
show the reaction diagram
the 54 kDa isoform of calpain-10 proteolyzes SNAP-25, a member of SNARE family of proteins involved in vesicle fusion
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-
?
peptide + H2O
hydrolyzed peptide
show the reaction diagram
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genetic variation in the calpain 10 gene is associated with an increased risk of type II diabetes mellitus in humans
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-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
Q9HC96
-
-
-
?
SNAP-25 + H2O
?
show the reaction diagram
Q9HC96
the 54 kDa isoform of calpain-10 proteolyzes SNAP-25, a member of SNARE family of proteins involved in vesicle fusion
-
-
?
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
genetic variation in the calpain 10 gene is associated with an increased risk of type II diabetes mellitus in humans
-
-
?
additional information
?
-
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mitochondrial calpain 10 is required for cell viability. Calpain 10 levels specifically decrease in aging human kidney tissue when renal function decreases, suggesting that calpain 10 is required for renal function and is a biomarker of the aging kidney
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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dependent on
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calpastatin
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-
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N-[(2R)-4-hydroxy-1-oxobutan-2-yl]-N2-(phenylcarbamothioyl)-L-leucinamide
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binding structure, the inhibitor shows interactions with the Cys115, Gly207, Gly20,8 and Gly271 residues, overview. Reversible intramolecular conversion of the cyclic hemiacetal form of SNJ-1715 to the free aldehyde form
N-[(3R)-2-hydroxytetrahydrofuran-3-yl]-N2-(phenylcarbamothioyl)-L-leucinamide
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binding structure, the inhibitor shows interactions with the Cys115, Gly207, Gly20,8 and Gly271 residues, overview. Reversible intramolecular conversion of the cyclic hemiacetal form of SNJ-1715 to the free aldehyde form
additional information
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pan-calpain inhibitors reduce AngII-induced aldosterone production in 2 adrenal glomerulosa cell models, primary bovine zona glomerulosa and human adrenocortical carcinoma cells
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
angiotensin II
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activates the enzyme in vivo, reduced by pan-calpain inhibitors
Ca2+
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glucose
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gradual increase after 3.5 days of exposure
intralipid
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fat emulsion consisting of 12% palmitic acid, 4% stearic acid, 21% oleic acid, 53% linoleic acid, 7% alpha-linoleic acid, and 3% other acids, after 24 h exposure, increase in CAPN10 mRNA in response to insulin in subjects with normal glucose tolerance
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
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two major isoforms with intact N-terminus but different C-terminal T domains
Manually annotated by BRENDA team
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calpain 10 protein and mRNA levels decrease linearly in human kidney samples with age
Manually annotated by BRENDA team
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lens epithelium, outer fiber cells of lens
Manually annotated by BRENDA team
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it is proposed that a defective CAPN10 pre-mRNA processing is responsible for the decreased levels of SNP-43 A-allele transcripts in peripheral white cells of healthy and T2D individuals
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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mitochondrial calpain 10 is required for cell viability
Manually annotated by BRENDA team
PDB
SCOP
CATH
UNIPROT
ORGANISM
-
-
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64000
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in the cytosol, SDS-PAGE
additional information
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immuno-stained bands at 26000, 54000 and 72000 Da between cytosol and membranes, SDS-PAGE, the 54000 Da isoform is associated with a synaptosomal-associated protein of 25000 Da
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 54000, SDS-PAGE
monomer
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in INS-1 cells
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genotyping and association with type 2 diabetes mellitus , detailed overview
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
calpain-10 is overexpressed and activated through the inhibition of ryanodine receptor 2 under hypoglycemic and high fatty acid conditions, causing caspase-3-independent beta-cell death
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calpain-10 expression is elevated by 64% in pancreatic islets from patients with type 2 diabetes compared with non-diabetic donors
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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the development of inhibitors against calpain-10 may be useful as therapeutic agents for a number of calpainopathies
medicine
medicine