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Information on EC 3.4.22.B30 - calpain 10 and Organism(s) Homo sapiens and UniProt Accession Q9HC96

for references in articles please use BRENDA:EC3.4.22.B30
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B30 calpain 10
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q9HC96
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
broad endopeptidase specificity
Synonyms
capn10, calpain-10, calpain 10, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calpain-10
CAPN10
CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
-
-
?
SNAP-25 + H2O
?
show the reaction diagram
the 54 kDa isoform of calpain-10 proteolyzes SNAP-25, a member of SNARE family of proteins involved in vesicle fusion
-
-
?
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
genetic variation in the calpain 10 gene is associated with an increased risk of type II diabetes mellitus in humans
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
-
-
?
SNAP-25 + H2O
?
show the reaction diagram
the 54 kDa isoform of calpain-10 proteolyzes SNAP-25, a member of SNARE family of proteins involved in vesicle fusion
-
-
?
peptide + H2O
hydrolyzed peptide
show the reaction diagram
-
genetic variation in the calpain 10 gene is associated with an increased risk of type II diabetes mellitus in humans
-
-
?
additional information
?
-
-
mitochondrial calpain 10 is required for cell viability. Calpain 10 levels specifically decrease in aging human kidney tissue when renal function decreases, suggesting that calpain 10 is required for renal function and is a biomarker of the aging kidney
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
dependent on
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calpastatin
-
-
N-[(2R)-4-hydroxy-1-oxobutan-2-yl]-N2-(phenylcarbamothioyl)-L-leucinamide
binding structure, the inhibitor shows interactions with the Cys115, Gly207, Gly20,8 and Gly271 residues, overview. Reversible intramolecular conversion of the cyclic hemiacetal form of SNJ-1715 to the free aldehyde form
N-[(3R)-2-hydroxytetrahydrofuran-3-yl]-N2-(phenylcarbamothioyl)-L-leucinamide
binding structure, the inhibitor shows interactions with the Cys115, Gly207, Gly20,8 and Gly271 residues, overview. Reversible intramolecular conversion of the cyclic hemiacetal form of SNJ-1715 to the free aldehyde form
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
angiotensin II
activates the enzyme in vivo, reduced by pan-calpain inhibitors
glucose
gradual increase after 3.5 days of exposure
intralipid
fat emulsion consisting of 12% palmitic acid, 4% stearic acid, 21% oleic acid, 53% linoleic acid, 7% alpha-linoleic acid, and 3% other acids, after 24 h exposure, increase in CAPN10 mRNA in response to insulin in subjects with normal glucose tolerance
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
it is proposed that a defective CAPN10 pre-mRNA processing is responsible for the decreased levels of SNP-43 A-allele transcripts in peripheral white cells of healthy and T2D individuals
Manually annotated by BRENDA team
-
two major isoforms with intact N-terminus but different C-terminal T domains
Manually annotated by BRENDA team
-
calpain 10 protein and mRNA levels decrease linearly in human kidney samples with age
Manually annotated by BRENDA team
-
lens epithelium, outer fiber cells of lens
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
mitochondrial calpain 10 is required for cell viability
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
physiological function
-
the calpain-10 expression correlates positively with arginine-stimulated insulin release in islets from non-diabetic donors
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN10_HUMAN
672
0
74952
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
64000
in the cytosol, SDS-PAGE
additional information
immuno-stained bands at 26000, 54000 and 72000 Da between cytosol and membranes, SDS-PAGE, the 54000 Da isoform is associated with a synaptosomal-associated protein of 25000 Da
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 54000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in INS-1 cells
genotyping and association with type 2 diabetes mellitus , detailed overview
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
calpain-10 is overexpressed and activated through the inhibition of ryanodine receptor 2 under hypoglycemic and high fatty acid conditions, causing caspase-3-independent beta-cell death
calpain-10 expression is elevated by 64% in pancreatic islets from patients with type 2 diabetes compared with non-diabetic donors
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
the development of inhibitors against calpain-10 may be useful as therapeutic agents for a number of calpainopathies
medicine
medicine
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q9HC96), Macaca fascicularis (Q95LP4), Mus musculus (Q9ESK3), Mus musculus 129/SvJ (Q9ESK3), Rattus norvegicus (Q9ES66)
Manually annotated by BRENDA team
Strausberg R.L.; Feingold E.A.; Grouse L.H.; Derge J.G.; et al.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
Proc. Natl. Acad. Sci. USA
99
16899-16903
2002
Homo sapiens (Q9HC96), Mus musculus (Q9ESK3)
Manually annotated by BRENDA team
Horikawa, Y.; Oda, N.; Cox, N.J.; Li, X.; et al.
Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus
Nat. Genet.
26
163-75
2000
Mus musculus (Q9ESK3), Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
Nagase, T.; Nakayama, M.; Nakajima, D.; Kikuno, R.; Ohara, O.
Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro
DNA Res.
8
85-95
2001
Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
Ma, H.; Fukiage, C.; Kim, Y.H.; Duncan, M.K.; Reed, N.E.
Sih, M.; Azuma, M.; Shearer, T.R.: Characterization and expression of calpain 10. A novel ubiquitous calpain within nuclear localization
J. Biol. Chem.
276
28525-28531
2001
Homo sapiens, Mus musculus, Rattus norvegicus (Q9ES66)
Manually annotated by BRENDA team
Carlsson, E.; Poulsen, P.; Storgaard, H.; Almgren, P.; Ling, C.; Jensen, C.B.; Madsbad, S.; Groop, L.; Vaag, A.; Ridderstrale, M.
Genetic and nongenetic regulation of CAPN10 mRNA expression in skeletal muscle
Diabetes
54
3015-3020
2005
Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
Turner, M.D.; Cassell, P.G.; Hitman, G.A.
Calpain-10: from genome search to function
Diabetes Metab. Res. Rev.
21
505-514
2005
Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
Marshall, C.; Hitman, G.A.; Partridge, C.J.; Clark, A.; Ma, H.; Shearer, T.R.; Turner, M.D.
Evidence that an isoform of calpain-10 is a regulator of exocytosis in pancreatic beta-cells
Mol. Endocrinol.
19
213-224
2005
Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
Vollmert, C.; Hahn, S.; Lamina, C.; Huth, C.; Kolz, M.; Schoepfer-Wendels, A.; Mann, K.; Bongardt, F.; Mueller, J.C.; Kronenberg, F.; Wichmann, H.E.; Herder, C.; Holle, R.; Loewel, H.; Illig, T.; Janssen, O.E.; Janssen, O.E.
Calpain-10 variants and haplotypes are associated with polycystic ovary syndrome in Caucasians
Am. J. Physiol. Endocrinol. Metab.
292
E836-E844
2007
Homo sapiens
Manually annotated by BRENDA team
Lopez-Orduna, E.; Garcia-Mena, J.; Garcia-Macedo, R.; Stumvoll, M.; Cruz, M.
CAPN10 mRNA splicing and decay is not affected by a SNP associated with susceptibility to type 2 diabetes
Biochem. Biophys. Res. Commun.
358
831-836
2007
Homo sapiens (Q9HC96), Homo sapiens
Manually annotated by BRENDA team
Norton, L.; Parr, T.; Chokkalingam, K.; Bardsley, R.G.; Ye, H.; Bell, G.I.; Pelsers, M.M.; van Loon, L.J.; Tsintzas, K.
Calpain-10 gene and protein expression in human skeletal muscle: effect of acute lipid-induced insulin resistance and type 2 diabetes
J. Clin. Endocrinol. Metab.
93
992-998
2008
Homo sapiens
Manually annotated by BRENDA team
Covington, M.D.; Arrington, D.D.; Schnellmann, R.G.
Calpain 10 is required for cell viability and is decreased in the aging kidney
Am. J. Physiol. Renal Physiol.
296
F478-F486
2009
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Dong, B.; Liu, R.
Characterization of endogenous and recombinant human calpain-10
Biochimie
90
1362-1371
2008
Homo sapiens
Manually annotated by BRENDA team
Lee, J.Y.; Lee, W.J.; Hur, S.E.; Lee, C.M.; Sung, Y.A.; Chung, H.W.
111/121 diplotype of Calpain-10 is associated with the risk of polycystic ovary syndrome in Korean women
Fertil. Steril.
92
830-833
2008
Homo sapiens
Manually annotated by BRENDA team
Ezzidi, I.; Turki, A.; Messaoudi, S.; Chaieb, M.; Kacem, M.; Al-Khateeb, G.M.; Mahjoub, T.; Almawi, W.Y.; Mtiraoui, N.
Common polymorphisms of calpain-10 and the risk of Type 2 Diabetes in a Tunisian Arab population: a case-control study
BMC Med. genet.
11
75
2010
Homo sapiens
Manually annotated by BRENDA team
Zhou, X.; Wang, Y.; Zhang, Y.; Gao, P.; Zhu, D.
Association of CAPN10 gene with insulin sensitivity, glucose tolerance and renal function in essential hypertensive patients
Clin. Chim. Acta
411
1126-1131
2010
Homo sapiens
Manually annotated by BRENDA team
Adak, S.; Sengupta, S.; Chowdhury, S.; Bhattacharyya, M.
Co-existence of risk and protective haplotypes of Calpain 10 gene to type 2 diabetes in the eastern Indian population
Diab. Vasc. Dis. Res.
7
63-68
2010
Homo sapiens
Manually annotated by BRENDA team
Ezzidi, I.; Mtiraoui, N.; Nemr, R.; Kacem, M.; Al-Khateeb, G.M.; Mahjoub, T.; Almawi, W.Y.
Variants within the calpain-10 gene and relationships with type 2 diabetes (T2DM) and T2DM-related traits among Tunisian Arabs
Diabetes Metab.
36
357-362
2010
Homo sapiens
Manually annotated by BRENDA team
Moreno-Luna, R.; Abrante, A.; Esteban, F.; Gonzalez-Moles, M.A.; Delgado-Rodriguez, M.; Saez, M.E.; Gonzalez-Perez, A.; Ramirez-Lorca, R.; Real, L.M.; Ruiz, A.
Calpain 10 gene and laryngeal cancer: A survival analysis
Head Neck
33
72-76
2011
Homo sapiens
Manually annotated by BRENDA team
Alsaraj, F.; OGorman, D.; McAteer, S.; McDermott, J.; Hawi, Z.; Sreenan, S.
Haplotype association of calpain 10 gene variants with type 2 diabetes mellitus in an Irish sample
Ir. J. Med. Sci.
179
269-272
2010
Homo sapiens
Manually annotated by BRENDA team
Unsal, T.; Konac, E.; Yesilkaya, E.; Yilmaz, A.; Bideci, A.; Ilke Onen, H.; Cinaz, P.; Menevse, A.
Genetic polymorphisms of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes in adolescent girls with polycystic ovary syndrome
J. Assist. Reprod. Genet.
26
205-216
2009
Homo sapiens
Manually annotated by BRENDA team
Fong, P.Y.; Fesinmeyer, M.D.; White, E.; Farin, F.M.; Srinouanprachanh, S.; Afsharinejad, Z.; Mandelson, M.T.; Brentnall, T.A.; Barnett, M.J.; Goodman, G.E.; Austin, M.A.
Association of diabetes susceptibility gene calpain-10 with pancreatic cancer among smokers
J. Gastrointest. Cancer
41
203-208
2010
Homo sapiens
Manually annotated by BRENDA team
Bodhini, D.; Radha, V.; Ghosh, S.; Sanapala, K.R.; Majumder, P.P.; Rao, M.R.; Mohan, V.
Association of calpain 10 gene polymorphisms with type 2 diabetes mellitus in Southern Indians
Metab. Clin. Exp.
60
681-688
2011
Homo sapiens
Manually annotated by BRENDA team
Kurzawski, M.; Dziewanowski, K.; Kedzierska, K.; Gornik, W.; Banas, A.; Drozdzik, M.
Association of calpain-10 gene polymorphism and posttransplant diabetes mellitus in kidney transplant patients medicated with tacrolimus
Pharmacogenomics J.
10
120-125
2010
Homo sapiens
Manually annotated by BRENDA team
Ling, C.; Groop, L.; Guerra, S.D.; Lupi, R.
Calpain-10 expression is elevated in pancreatic islets from patients with type 2 diabetes
PLoS ONE
4
e6558
2009
Homo sapiens
Manually annotated by BRENDA team
Perez-Martinez, P.; Delgado-Lista, J.; Garcia-Rios, A.; Ferguson, J.F.; Gulseth, H.L.; Williams, C.M.; Karlstroem, B.; Kiec-Wilk, B.; Blaak, E.E.; Helal, O.; Malczewska-Malec, M.; Defoort, C.; Riserus, U.; Saris, W.H.; Lovegrove, J.A.; Drevon, C.A.; Roche, H.M.; Lopez-Miranda, J.
Calpain-10 interacts with plasma saturated fatty acid concentrations to influence insulin resistance in individuals with the metabolic syndrome
Am. J. Clin. Nutr.
93
1136-1141
2011
Homo sapiens
Manually annotated by BRENDA team
Panico, P.; Salazar, A.M.; Burns, A.L.; Ostrosky-Wegman, P.
Role of calpain-10 in the development of diabetes mellitus and its complications
Arch. Med. Res.
45
103-115
2014
Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
da Silva, R.C.; de Alencar, N.A.; Alves, C.N.; Lameira, J.
Analysis of the structure of calpain-10 and its interaction with the protease inhibitor SNJ-1715
Comput. Biol. Med.
43
1334-1340
2013
Homo sapiens (Q9HC96), Homo sapiens
Manually annotated by BRENDA team
Seremwe, M.; Schnellmann, R.G.; Bollag, W.B.
Calpain-10 activity underlies angiotensin II-induced aldosterone production in an adrenal glomerulosa cell model
Endocrinology
156
2138-2149
2015
Homo sapiens (Q9HC96), Homo sapiens
Manually annotated by BRENDA team
Oladnabi, M.; Musante, L.; Larti, F.; Hu, H.; Abedini, S.S.; Wienker, T.; Ropers, H.H.; Kahrizi, K.; Najmabadi, H.
New evidence for the role of calpain 10 in autosomal recessive intellectual disability identification of two novel nonsense variants by exome sequencing in Iranian families
Arch. Iran Med.
18
179-184
2015
Homo sapiens (Q9HC96), Homo sapiens
Manually annotated by BRENDA team
Chaudhry, B.; Hanif, F.; Saboohi, K.
Molecular signatures of Calpain 10 isoforms sequences, envisage functional similarity and therapeutic potential
Pak. J. Pharm. Sci.
32
937-946
2019
Homo sapiens (Q9HC96)
Manually annotated by BRENDA team