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Information on EC 3.4.22.B30 - calpain 10 and Organism(s) Mus musculus and UniProt Accession Q9ESK3

for references in articles please use BRENDA:EC3.4.22.B30
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B30 calpain 10
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This record set is specific for:
Mus musculus
UNIPROT: Q9ESK3
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide
broad endopeptidase specificity
Synonyms
capn10, calpain-10, calpain 10, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calpain-10
CAPN10
CAS REGISTRY NUMBER
COMMENTARY hide
78990-62-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
ATP synthase beta + H2O
?
show the reaction diagram
-
-
-
-
?
NDUFB8 + H2O
?
show the reaction diagram
-
-
-
-
?
SLLVY-7-amido-4-methylcoumarin + H2O
SLLVY + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
mitochondrial calpain 10 is required for cell viability. Calpain 10 levels specifically decreases in aging mouse kidney tissue when renal function decreases, suggesting that calpain 10 is required for renal function and is a biomarker of the aging kidney
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
peptide + H2O
hydrolyzed peptide
show the reaction diagram
proteolysis of substrates involved in cytoskeletal remodelling and signal transduction
-
-
?
ATP synthase beta + H2O
?
show the reaction diagram
-
-
-
-
?
NDUFB8 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
mitochondrial calpain 10 is required for cell viability. Calpain 10 levels specifically decreases in aging mouse kidney tissue when renal function decreases, suggesting that calpain 10 is required for renal function and is a biomarker of the aging kidney
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
calcium-regulated protein
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calpeptin
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in Capn10-/- mouse embryonic fibroblasts, MAP1B is localized at actin filaments rather than at microtubules
Manually annotated by BRENDA team
in pancreatic islets from Calpain-10 knockout mice, insulin secretion is significantly increased both at the high and low glucose levels
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Calpain-10 processes microtubule associated protein 1 (MAP1) family proteins into heavy and light chains and regulates their binding activities to microtubules and actin filaments
Manually annotated by BRENDA team
-
cultured alphaTN4-1 lens epithelial cells
Manually annotated by BRENDA team
-
cultured alphaTN4-1 lens epithelial cells
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
Calpain-10 processes microtubule associated protein 1 (MAP1) family proteins into heavy and light chains and regulates their binding activities to microtubules and actin filaments. In Capn10-/- mouse embryonic fibroblasts, MAP1B is localized at actin filaments rather than at microtubules. Calpain-10 regulates actin dynamics via MAP1B cleavage. In pancreatic islets from Calpain-10 knockout mice, insulin secretion is significantly increased both at the high and low glucose levels
malfunction
physiological function
-
Capn10 plays an important role in regulating obesity and diabetes in mice
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAN10_MOUSE
666
0
74596
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
-
x * 75000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 75000, SDS-PAGE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
renal calpain 10 protein and mRNA are specifically decreased in ob/ob diabetic mice
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q9HC96), Macaca fascicularis (Q95LP4), Mus musculus (Q9ESK3), Mus musculus 129/SvJ (Q9ESK3), Rattus norvegicus (Q9ES66)
Manually annotated by BRENDA team
Strausberg R.L.; Feingold E.A.; Grouse L.H.; Derge J.G.; et al.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
Proc. Natl. Acad. Sci. USA
99
16899-16903
2002
Homo sapiens (Q9HC96), Mus musculus (Q9ESK3)
Manually annotated by BRENDA team
Okazaki, Y.; Furuno, M.; Kasukawa, T.; Adachi, J.; Bono, H.; et al.
Analysis of the mouse transcriptome based on functional annotation of 60770 full-length cDNAs
Nature
5; 420
563-73
2002
Mus musculus (Q9ESK3), Mus musculus NOD (Q9ESK3)
-
Manually annotated by BRENDA team
Horikawa, Y.; Oda, N.; Cox, N.J.; Li, X.; et al.
Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus
Nat. Genet.
26
163-75
2000
Mus musculus (Q9ESK3), Homo sapiens (Q9HC96)
Manually annotated by BRENDA team
Ma, H.; Fukiage, C.; Kim, Y.H.; Duncan, M.K.; Reed, N.E.
Sih, M.; Azuma, M.; Shearer, T.R.: Characterization and expression of calpain 10. A novel ubiquitous calpain within nuclear localization
J. Biol. Chem.
276
28525-28531
2001
Homo sapiens, Mus musculus, Rattus norvegicus (Q9ES66)
Manually annotated by BRENDA team
Giguere, C.J.; Covington, M.D.; Schnellmann, R.G.
Mitochondrial calpain 10 activity and expression in the kidney of multiple species
Biochem. Biophys. Res. Commun.
366
258-262
2008
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Manually annotated by BRENDA team
Covington, M.D.; Arrington, D.D.; Schnellmann, R.G.
Calpain 10 is required for cell viability and is decreased in the aging kidney
Am. J. Physiol. Renal Physiol.
296
F478-F486
2009
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Cheverud, J.M.; Fawcett, G.L.; Jarvis, J.P.; Norgard, E.A.; Pavlicev, M.; Pletscher, L.S.; Polonsky, K.S.; Ye, H.; Bell, G.I.; Semenkovich, C.F.
Calpain-10 is a component of the obesity-related quantitative trait locus Adip1
J. Lipid Res.
51
907-913
2010
Mus musculus
Manually annotated by BRENDA team
Covington, M.D.; Schnellmann, R.G.
Chronic high glucose downregulates mitochondrial calpain 10 and contributes to renal cell death and diabetes-induced renal injury
Kidney Int.
81
391-400
2012
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Hatta, T.; Iemura, S.I.; Ohishi, T.; Nakayama, H.; Seimiya, H.; Yasuda, T.; Iizuka, K.; Fukuda, M.; Takeda, J.; Natsume, T.; Horikawa, Y.
Calpain-10 regulates actin dynamics by proteolysis of microtubule-associated protein 1B
Sci. Rep.
8
16756
2018
Mus musculus (Q9ESK3), Mus musculus
Manually annotated by BRENDA team