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Information on EC 3.4.22.B30 - calpain 10 and Organism(s) Rattus norvegicus and UniProt Accession Q9ES66
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3.4.22.B30 calpain 10Specify your search results
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- 3.4.22.B30
- calpains
- mellitus
-
ucsnp-43
-
polycystic
-
tcf7l2
-
mexican
-
kcnj11
- medicine
-
mexican-american
-
pro12ala
-
7-like
-
igf2bp2
-
haplogenotype
- cdkal1
- drug development
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
capn10, calpain-10, calpain 10, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
calpain-10
-
-
-
-
CAPN10
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
78990-62-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
SLLVY-7-amido-4-methylcoumarin + H2O
SLLVY + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
peptide + H2O
hydrolyzed peptide
calpain 10 transcripts are rapidly up-regulated in the outer regions of the lens during nuclear cataract formation, but calpain 10 protein is degraded when cataract is present
-
-
?
SNAP-25 + H2O
?
-
i.e. synaptosome-associated protein of 25000 Da. One of the mechanisms by which arsenite impairs insulin secretion is by decreasing the oscillations of free Ca2+, thus reducing calcium-dependent calpain-10 partial proteolysis of SNAP-25
-
-
?
additional information
?
-
DAP13 (13 kDa differentiation-associated protein) is not hydrolyzed by calpain 10 in the presence of CaCl2
-
-
?
additional information
?
-
-
mitochondrial calpain 10 is required for cell viability. Calpain 10 levels specifically decrease in aging rat kidney tissue when renal function decreases, suggesting that calpain 10 is required for renal function and is a biomarker of the aging kidney
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
SNAP-25 + H2O
?
-
i.e. synaptosome-associated protein of 25000 Da. One of the mechanisms by which arsenite impairs insulin secretion is by decreasing the oscillations of free Ca2+, thus reducing calcium-dependent calpain-10 partial proteolysis of SNAP-25
-
-
?
additional information
?
-
-
mitochondrial calpain 10 is required for cell viability. Calpain 10 levels specifically decrease in aging rat kidney tissue when renal function decreases, suggesting that calpain 10 is required for renal function and is a biomarker of the aging kidney
-
-
?
peptide + H2O
hydrolyzed peptide
calpain 10 transcripts are rapidly up-regulated in the outer regions of the lens during nuclear cataract formation, but calpain 10 protein is degraded when cataract is present
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
CYGAK disulfide
-
valuable tool to prevent Ca2+ induced mitochondrial dysfunction and explore the function of calpain 10
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
several splice variants of calpain 10 are localized to the mitochondria in kidneys
-
calpain 10 protein decreases in rat kidney samples with age, this decrease is attenuated with caloric restriction
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
mitochondrial outer membrane, intermembrane space, inner membrane, matrix
-
several splice variants of calpain 10 are localized to the mitochondria in kidney
-
mitochondrial calpain 10 is required for cell viability
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
knockdown of calpain 10 results in decreased renal function as evidenced by increased serum creatinine, and increased caspase 3 cleavage
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
-
x * 50000, diverse isoforms of calpain-10 are produced by alternative splicing. The isoform that shows the highest levels of expression is the one of approximately 50000 Da
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 50000, diverse isoforms of calpain-10 are produced by alternative splicing. The isoform that shows the highest levels of expression is the one of approximately 50000 Da
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
chronic high glucose down-regulates mitochondrial calpain 10. High glucose (17 mM) decreases mitochondrial calpain 10 mRNA and protein at 96 h compared with cells incubated with 0 or 5 mM glucose or 17 mM D-mannitol. Renal calpain 10 protein and mRNA are specifically decreased in streptozotocin-induced diabetic rats with kidney dysfunction
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mitochondrial calpain activity does not change with 5mM glucose or 17 mM D-mannitol
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q9HC96), Macaca fascicularis (Q95LP4), Mus musculus (Q9ESK3), Mus musculus 129/SvJ (Q9ESK3), Rattus norvegicus (Q9ES66)
Ma, H.; Fukiage, C.; Kim, Y.H.; Duncan, M.K.; Reed, N.E.
Sih, M.; Azuma, M.; Shearer, T.R.: Characterization and expression of calpain 10. A novel ubiquitous calpain within nuclear localization
J. Biol. Chem.
276
28525-28531
2001
Homo sapiens, Mus musculus, Rattus norvegicus (Q9ES66)
Arrington, D.; Van Vleet, T.; Schnellmann, R.
Calpain 10: a mitochondrial calpain and its role in calcium-induced mitochondrial dysfunction
Am. J. Physiol. Cell Physiol.
291
C1159-1171
2006
Oryctolagus cuniculus, Rattus norvegicus (Q9ES66)
Giguere, C.J.; Covington, M.D.; Schnellmann, R.G.
Mitochondrial calpain 10 activity and expression in the kidney of multiple species
Biochem. Biophys. Res. Commun.
366
258-262
2008
Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
Covington, M.D.; Arrington, D.D.; Schnellmann, R.G.
Calpain 10 is required for cell viability and is decreased in the aging kidney
Am. J. Physiol. Renal Physiol.
296
F478-F486
2009
Homo sapiens, Mus musculus, Rattus norvegicus
Rasbach, K.A.; Arrington, D.D.; Odejinmi, S.; Giguere, C.; Beeson, C.C.; Schnellmann, R.G.
Identification and optimization of a novel inhibitor of mitochondrial calpain 10
J. Med. Chem.
52
181-188
2009
Rattus norvegicus
Diaz-Villasenor, A.; Burns, A.L.; Salazar, A.M.; Sordo, M.; Hiriart, M.; Cebrian, M.E.; Ostrosky-Wegman, P.
Arsenite reduces insulin secretion in rat pancreatic beta-cells by decreasing the calcium-dependent calpain-10 proteolysis of SNAP-25
Toxicol. Appl. Pharmacol.
231
291-299
2008
Rattus norvegicus
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