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Information on EC 3.4.22.B1 - vignain and Organism(s) Vigna mungo and UniProt Accession P12412

for references in articles please use BRENDA:EC3.4.22.B1
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B1 vignain
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This record set is specific for:
Vigna mungo
UNIPROT: P12412
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Word Map
The taxonomic range for the selected organisms is: Vigna mungo
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hydrolysis of proteins, such as azocasein. Preferential cleavage: Asn-/-Xaa in small molecule substrates such as Boc-Asn-/-OPHNO2
Synonyms
sh-ep, cyp15a, cysep, vmpe-1, cpph1, sulfhydryl-endopeptidase, vignain, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Bean endopeptidase
-
-
-
-
C01.010
-
-
-
-
cysteine proteinase
-
-
-
-
SH-EP
-
-
-
-
sulfhydryl-endopeptidase
-
-
-
-
VmPE-1
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
149371-19-7
-
229473-96-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
-
-
-
?
Boc-Asn 4-nitrophenyl ester + H2O
Boc-Asn + 4-nitrophenol
show the reaction diagram
-
-
-
?
additional information
?
-
-
the enzyme acts to degrade the seed storage protein in protein storage vacuoles
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme acts to degrade the seed storage protein in protein storage vacuoles
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
the enzyme is synthesized on membrane-bound ribosomes as an inactive 45000 Da precursor
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CYSEP_VIGMU
362
0
40222
Swiss-Prot
other Location (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Vigna mungo (P12412)
Manually annotated by BRENDA team
Akasofu H.; Yamauchi D.; Mitsuhashi W.; Minamikawa T.
Nucleotide sequence of cDNA for sulfhydryl-endopeptidase (SH-EP) from cotyledons of germinating Vigna mungo seeds
Nucleic Acids Res.
17
6733-6733
1989
Vigna mungo (P12412), Vigna mungo
Manually annotated by BRENDA team
Akasofu H.; Yamauchi D.; Minamikawa T.
Nucleotide sequence of the gene for the Vigna mungo sulfhydryl-endopeptidase (SH-EP)
Nucleic Acids Res.
18
1892-1892
1990
Vigna mungo (P12412), Vigna mungo
Manually annotated by BRENDA team
Okamoto, T.; Minamikawa, T.
Purification of a processing enzyme (VmPE-1) that is involved in post-translational processing of a plant cysteine endopeptidase (SH-EP)
Eur. J. Biochem.
231
300-305
1995
Vigna mungo
Manually annotated by BRENDA team
Mitsuhashi, W.; Minamikawa, T.
Synthesis and posttranslational activation of sulfhydryl-endopeptidase in cotyledons of germinating Vigna mungo seeds
Plant Physiol.
89
274-279
1989
Vigna mungo
Manually annotated by BRENDA team
Okamoto, T.; Toyooka, K.; Minamikawa, T.
Identification of a membrane-associated cysteine protease with possible dual roles in the endoplasmic reticulum and protein storage vacuole
J. Biol. Chem.
276
742-751
2001
Vigna mungo
Manually annotated by BRENDA team