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Information on EC 3.4.22.61 - caspase-8 and Organism(s) Rattus norvegicus and UniProt Accession Q9JHX4

for references in articles please use BRENDA:EC3.4.22.61
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.61 caspase-8
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q9JHX4 not found.
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The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-/-(Gly/Ser/Ala)
Synonyms
caspase-8, caspase 8, casp8, flice, cysteine protease caspase-8, cysteine aspartic acid-specific protease, apoptotic cysteine protease, flice/mach, fadd-like ice, cysteine aspartic acid protease 8, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
apoptotic cysteine protease
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-
-
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apoptotic protease Mch-5
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-
-
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C14.004
-
-
-
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CAP4
-
-
-
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caspase 8
FADD-homologous ICE/CED-3-like protease
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-
-
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FADD-like ICE
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-
-
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FLICE
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-
-
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FLICE/MACH
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-
-
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ICE-like apoptotic protease 5
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-
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MACH
-
-
-
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Mch5
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-
-
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MORT1-associated CED-3 homolog
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
179241-78-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
FLICE + H2O
?
show the reaction diagram
37°C
-
-
?
IETD-4-nitroanilide + H2O
IETD + 4-nitroaniline
show the reaction diagram
-
-
-
?
procaspase-3 + H2O
active caspase-3 + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
procaspase-3 + H2O
active caspase-3 + ?
show the reaction diagram
-
direct activation by caspase-8 in type I cells
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Z-IETD-fluoromethyl ketone
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benzyloxycarbonyl-IETD-fluoromethylketone
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carboxyfluorescein-LETD-fluoromethylketone
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tumor necrosis factor-alpha
increases activity after 24 h exposure
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TNF-alpha/irradiation
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leads to an increase of caspase-8 activity up to 3.2fold within 24 h and up to 4.4fold within 48 h after irradiation, administration of TNF-alpha to non-irradiated hepatocytes does not lead to an increased activity of caspase-8
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additional information
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irradiation induces TNF-alpha-mediated activation of caspase-8, and apoptosis in hepatocytes. Apoptosis induction is prevented by IkappaB antisense oligonucleotides mediated by suppression of caspases-9 and -3 activation but not of caspase-8 activation, overview
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
adult ventricular myocyte
Manually annotated by BRENDA team
additional information
-
type I cells generate high levels of caspase-8
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
co-localization with Fas death domain, FADD
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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silencing of caspase 8 gene is a key factor controlling the outcome of neonatal astrocytes upon Fas engagement, restoration of caspase 8 expression triggers apoptotic cell death in primary neonatal astrocytes
physiological function
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activation of caspase 8 is involved in Fas-induced apoptosis in neonatal astrocytes
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CASP8_RAT
482
0
55339
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
cleaved caspase 8, SDS-PAGE
57000
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pro-caspase 8, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
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upon co-localization with Fas death domain, FADD, high, localized concentrations of procaspase-8 undergo autoproteolytic cleavage, releasing activated caspase-8
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
mRNA levels, protein levels and activity of caspase-8 is increased in a dose-dependent manner in livers (up to 2.14fold) and lungs (up to 2.12fold) of rats after SO2 inhalation (treated with 14, 28, and 56 mg/m3 SO2 for 7 days at 6 h/day)
at the onset of heat acclimation (34°C, 2 days) increased caspase 8 activity confirms activation of the extrinsic (Fas ligand) apoptosis pathway
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caspase 8 mRNA is undetectable in neonatal astrocytes
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protein expression of precursor of caspase 8 is decreased 1.3fold in rats receiving 5mg/kg/day of perfluoroalkyl acid-perfluorononanoic acid
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treatment of neonatal astrocytes with the demethylating agent 5-aza-dC leads to an increase in caspase 8mRNA levels in originally caspase 8-negative neonatal astrocytes
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhu, J.; Liu, M.; Kennedy, R.H.; Liu, S.J.
TNF-alpha-induced impairment of mitochondrial integrity and apoptosis mediated by caspase-8 in adult ventricular myocytes
Cytokine
34
96-105
2006
Rattus norvegicus (Q9JHX4)
Manually annotated by BRENDA team
Reinehr, R.; Sommerfeld, A.; Keitel, V.; Grether-Beck, S.; Hauessinger, D.
Amplification of CD95 activation by caspase 8-induced endosomal acidification in rat hepatocytes
J. Biol. Chem.
283
2211-2222
2008
Rattus norvegicus
Manually annotated by BRENDA team
An, J.; Li, P.; Li, J.; Dietz, R.; Donath, S.
ARC is a critical cardiomyocyte survival switch in doxorubicin cardiotoxicity
J. Mol. Med.
87
401-410
2009
Rattus norvegicus
Manually annotated by BRENDA team
Guerleyen, H.; Christiansen, H.; Tello, K.; Dudas, J.; Hermann, R.M.; Rave-Fraenk, M.; Hess, C.F.; Ramadori, G.; Saile, B.
Irradiation leads to sensitization of hepatocytes to TNF-alpha-mediated apoptosis by upregulation of IkappaB expression
Radiat. Environ. Biophys.
48
85-94
2009
Rattus norvegicus
Manually annotated by BRENDA team
Assayag, M.; Gerstenblith, G.; Stern, M.D.; Horowitz, M.
Long- but not short-term heat acclimation produces an apoptosis-resistant cardiac phenotype: a lesson from heat stress and ischemic/reperfusion insults
Cell Stress Chaperones
15
651-664
2010
Rattus norvegicus
Manually annotated by BRENDA team
Bai, J.; Meng, Z.
Expression of caspase and apoptotic signal pathway induced by sulfur dioxide
Environ. Mol. Mutagen.
51
112-122
2010
Rattus norvegicus (Q9JHX4), Rattus norvegicus
Manually annotated by BRENDA team
Barca, O.; Carneiro, C.; Costoya, J.A.; Senaris, R.M.; Arce, V.M.
Resistance of neonatal primary astrocytes against Fas-induced apoptosis depends on silencing of caspase 8
Neurosci. Lett.
479
206-210
2010
Rattus norvegicus
Manually annotated by BRENDA team
Fang, X.; Feng, Y.; Wang, J.; Dai, J.
Perfluorononanoic acid-induced apoptosis in rat spleen involves oxidative stress and the activation of caspase-independent death pathway
Toxicology
267
54-59
2010
Rattus norvegicus
Manually annotated by BRENDA team