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Information on EC 3.4.22.56 - caspase-3 and Organism(s) Mus musculus and UniProt Accession P70677

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.56 caspase-3
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This record set is specific for:
Mus musculus
UNIPROT: P70677 not found.
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-/- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position
Synonyms
caspase-3, caspase 3, casp3, sca-1, cpp32, casp-3, cas-3, cpp-32, apopain, cgcaspase-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
apopain
-
-
-
-
C14.003
-
-
-
-
CASP-3
-
-
-
-
CASP3
-
-
caspase 3
caspase-3
-
-
CPP-32
-
-
-
-
CPP32
-
-
-
-
CPP32/apopain
-
-
-
-
cystein aspartic-specific protease-3
-
-
cysteine aspartic acid-specific protease
-
-
cysteine protease CPP32
-
-
-
-
IRP
-
-
-
-
SCA-1
-
-
-
-
SREBP cleavage activity 1
-
-
-
-
Yama protein
-
-
-
-
Yama/CPP32
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
169592-56-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
poly(ADP-ribose) polymerase + H2O
85000 Da fragment + ?
show the reaction diagram
caspase-3 is the predominant poly(ADP-ribose) polymerase cleaving enzyme
-
-
?
poly(ADP-ribose) polymerase + H2O
?
show the reaction diagram
-
-
-
?
transcription factor Pax7 + H2O
?
show the reaction diagram
19 S proteasome + H2O
?
show the reaction diagram
acetyl-Asp-Glu-Val-Asp-4-nitroanilide + H2O
acetyl-Asp-Glu-Val-Asp + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin + H2O
acetyl-Asp-Glu-Val-Asp + 7-amino-4-trifluoromethylcoumarin
show the reaction diagram
-
-
-
-
?
acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O
acetyl-Asp-Glu-Val-Asp + p-nitroaniline
show the reaction diagram
-
-
-
-
?
alpha-spectrin + H2O
?
show the reaction diagram
-
-
production of a 120 kDa fragment
-
?
Asp-Glu-Val-Asp-4-nitroanilide + H2O
Asp-Glu-Val-Asp + 4-nitroaniline
show the reaction diagram
-
caspase-3 specific substrate
-
-
?
beta-catenin + H2O
?
show the reaction diagram
-
processing of beta-catenin, production of a 70000 Da fragment
-
-
?
LLVY-4-methylcoumarin 7-amide + H2O
LLVY + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
procaspase-6 + H2O
caspase-6 + ?
show the reaction diagram
Rpt2 + H2O
?
show the reaction diagram
-
-
-
-
?
Rpt5 + H2O
?
show the reaction diagram
Rpt6 + H2O
?
show the reaction diagram
-
-
-
-
?
pre-interleukin-1beta + H2O
additional information
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
poly(ADP-ribose) polymerase + H2O
85000 Da fragment + ?
show the reaction diagram
caspase-3 is the predominant poly(ADP-ribose) polymerase cleaving enzyme
-
-
?
transcription factor Pax7 + H2O
?
show the reaction diagram
compensatory growth and regeneration of skeletal muscle is dependent on the resident stem cell population, satellite cells (SCs). Self-renewal and maintenance of the SC niche is coordinated by the paired-box transcription factor Pax7. Continued expression of this protein inhibits the myoblast differentiation program. Caspase 3 cleavage inactivation of Pax7 is a crucial step for terminating the self-renewal process
-
-
?
19 S proteasome + H2O
?
show the reaction diagram
-
caspase-3 specifically cleaves the 19 S component of 26 S proteasome Rpt2, Rpt6, and Rpn2 in skeletal muscle stimulating proteasome activity
-
-
?
beta-catenin + H2O
?
show the reaction diagram
-
processing of beta-catenin, production of a 70000 Da fragment
-
-
?
procaspase-6 + H2O
caspase-6 + ?
show the reaction diagram
-
activation requires caspase-3
-
-
?
Rpt2 + H2O
?
show the reaction diagram
-
-
-
-
?
Rpt5 + H2O
?
show the reaction diagram
-
in myoblasts, Rpt5 is sensitive to caspase-3-induced cleavage but in myotubes, it is not sensitive. Caspase-3 cleavage of Rpt5 is responsible for the difference in the patterns of proteasome subunit cleavage in myoblasts compared with myotubes
-
-
?
Rpt6 + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-({2-[5-tert-butyl-3-{[(4-methyl-1,2,5-oxadiazol-3-yl)methyl]amino}-2-oxopyrazin-1(2H)-yl]butanoyl}amino)-5-[methyl(pentyl)amino]-4-oxopentanoic acid
-
a chemical and reversible caspase-3 inhibitor, M867 with ionizing radiation in an in vivo mouse hind limb lung cancer model is well tolerated, and produces a significant tumor growth delay compared to radiation alone
DEVD-CHO
-
a potent caspase-3 inhibitor
FLICE-inhibitory proteins
-
some isoforms, including FLIPR inhibit Fas-mediated apoptosis by impeding caspase 3 activity as well as poly(ADP-ribose) polymerase cleavage
-
N-acetyl-Asp-Glu-Val-Asp-aldehyde
-
a caspase-3 inhibitor, i.e. DEVD-CHO, partially inhibits the DNA fragmentation and growth-inhibition induced by inotodiol
N-carbobenzyloxy-DEVD-fluoromethyl ketone
-
effects on the activation of caspase-3 through cell infection with Leptospira interrogans
N-carbobenzyloxy-VAD-fluoromethyl ketone
-
a general caspase inhibitor
Z-Asp-2,6-dichlorobenzoyloxymethylketone
-
a caspase-3-specific inhibitor
Z-VAD-fluoromethylketone
-
a pan-caspase inhibitor
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c
-
release of cytochrome c in vivo leads to increased caspase-3 activation in Daltons lymphoma cells
FADD
-
adapter molecule, required for caspase-3 activation
-
pancreatic derived factor
-
islet specific cytokine, capable of inducing apoptosis, increases levels of cleaved CASP3 in beta-TC3 cells
-
staurosporine
-
in myotubes, staurosporine increases proteasome activity by 38% and stimulates cleavage of proteasome subunits, Rpt2, Rpt6, and Rpn2. Rpt5 and Rpn10 subunits are not cleaved in staurosporine-treated myotubes
TNF-alpha
-
induces caspase-3 activation
-
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00012
M826
Mus musculus
-
IC50 for enzyme from cerebellar granule neuron: 0.00005 mM. IC50 for enzyme from cortical neuron: 0.00012 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
sensory neurons
Manually annotated by BRENDA team
-
insulinoma cell, treatment with islet-specific cytokine pancreatic derived factor results in elevated enzyme levels which abrogates expression of cyclin-dependent kinase inhibitor 1A
Manually annotated by BRENDA team
-
epithelial acinar cells
Manually annotated by BRENDA team
-
embryonic
Manually annotated by BRENDA team
-
main localization in the jejunum
Manually annotated by BRENDA team
-
hippocampal cell line, during glutamate-induced apoptotic cell death, glutamate induces activation of calpain, and translocation of mitochondrial apoptosis inducing factor AIF to cytosol and nuclei. Enzyme is not involved
Manually annotated by BRENDA team
-
geldanamycin inhibits hemorrhage-induced increases in caspase-3 activity
Manually annotated by BRENDA team
-
geldanamycin inhibits hemorrhage-induced increases in caspase-3 activity
Manually annotated by BRENDA team
-
peritoneal
Manually annotated by BRENDA team
-
leukemia cell
Manually annotated by BRENDA team
-
islet cell
Manually annotated by BRENDA team
-
epithelial acinar cells, nonobese diabetic mouse acinar cells at 16 weeks show increased caspase 3 activity
Manually annotated by BRENDA team
-
during differentiation of myoblasts to myotubes, there is an obligatory, transient increase in caspase-3 activity, accompanied by a corresponding increase in proteasome activity and cleavage of Rpt2 and Rpt6
Manually annotated by BRENDA team
additional information
-
beta-TC3 murine inulinoma cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
compensatory growth and regeneration of skeletal muscle is dependent on the resident stem cell population, satellite cells (SCs). Self-renewal and maintenance of the SC niche is coordinated by the paired-box transcription factor Pax7. Continued expression of this protein inhibits the myoblast differentiation program. Caspase 3 cleavage inactivation of Pax7 is a crucial step for terminating the self-renewal process
malfunction
metabolism
-
the stage of muscle cell differentiation determines not only the caspase-3-induced subunit cleavage patterns but also the changes in proteasome activity
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CASP3_MOUSE
277
0
31475
Swiss-Prot
other Location (Reliability: 2)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
construction of a caspase-3/-7 double-knockout mouse embryonic fibroblasts cell model for studying of autophagy contributing to the mechanism of increased cell death, following inhibition of apoptosis
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
caspase-3 is a marker of apoptotic cell death in embryonic fibroblasts, NIH-3T3 and WST-1 cells
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
van de Craen, M.; Berx, G.; van den Brande, I.; Fiers, W.; Declercq, W.; Vandenabeele, P.
Proteolytic cleavage of beta-catenin by caspases: an in vitro analysis
FEBS Lett.
458
167-170
1999
Mus musculus
Manually annotated by BRENDA team
van de Craen, M.; Vandenabeele, P.; Declercq, W.; van den Brande, I.; van Loo, G.; Molemans, F.; Schotte, P.; van Criekinge, W.; Beyaert, R.; Fiers, W.
Characterization of seven murine caspase family members
FEBS Lett.
403
61-69
1997
Mus musculus (P70677), Mus musculus, Mus musculus C3H/An (P70677)
Manually annotated by BRENDA team
Mukasa, T.; Urase, K.; Momoi, M.Y.; Kimura, I.; Momoi, T.
Specific expression of CPP32 in sensory neurons of mouse embryos and activation of CPP32 in the apoptosis induced by a withdrawal of NGF
Biochem. Biophys. Res. Commun.
231
770-774
1997
Mus musculus (P70677), Mus musculus
Manually annotated by BRENDA team
Djerbi, M.; Darreh-Shori, T.; Zhivotovsky, B.; Grandien, A.
Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different FLIP isoforms
Scand. J. Immunol.
54
180-189
2001
Mus musculus
Manually annotated by BRENDA team
Han, B.H.; Xu, D.; Choi, J.; Han, Y.; Xanthoudakis, S.; Roy, S.; Tam, J.; Vaillancourt, J.; Colucci, J.; Siman, R.; Giroux, A.; Robertson, G.S.; Zamboni, R.; Nicholson, D.W.; Holtzman, D.M.
Selective, reversible caspase-3 inhibitor is neuroprotective and reveals distinct pathways of cell death after neonatal hypoxic-ischemic brain injury
J. Biol. Chem.
277
30128-30136
2002
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Deveraux, Q.L.; Roy, N.; Stennicke, H.R.; Van Arsdale, T.; Zhou, Q.; Srinivasula, S.M.; Alnemri, E.S.; Salvesen, G.S.; Reed, J.C.
IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases
EMBO J.
17
2215-2223
1998
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Zhang, Y.; Bhavnani, B.R.
Glutamate-induced apoptosis in neuronal cells is mediated via caspase-dependent and independent mechanisms involving calpain and caspase-3 proteases as well as apoptosis inducing factor (AIF) and this process is inhibited by equine estrogens
BMC Neurosci.
7
49
2006
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Burkhardt, B.R.; Greene, S.R.; White, P.; Wong, R.K.; Brestelli, J.E.; Yang, J.; Robert, C.E.; Brusko, T.M.; Wasserfall, C.H.; Wu, J.; Atkinson, M.A.; Gao, Z.; Kaestner, K.H.; Wolf, B.A.
PANDER-induced cell-death genetic networks in islets reveal central role for caspase-3 and cyclin-dependent kinase inhibitor 1A (p21)
Gene
369
134-141
2006
Mus musculus, Mus musculus C57/BL6
Manually annotated by BRENDA team
Kiang, J.G.; Bowman, P.D.; Lu, X.; Li, Y.; Wu, B.W.; Loh, H.H.; Tsen, K.T.; Tsokos, G.C.
Geldanamycin inhibits hemorrhage-induced increases in caspase-3 activity: role of inducible nitric oxide synthase
J. Appl. Physiol.
103
1045-1055
2007
Mus musculus
Manually annotated by BRENDA team
Samuel, M.A.; Morrey, J.D.; Diamond, M.S.
Caspase 3-dependent cell death of neurons contributes to the pathogenesis of West Nile virus encephalitis
J. Virol.
81
2614-2623
2007
Mus musculus
Manually annotated by BRENDA team
Andersen, A.D.; Poulsen, K.A.; Lambert, I.H.; Pedersen, S.F.
HL-1 mouse cardiomyocyte injury and death after simulated ischemia and reperfusion: roles of pH, Ca2+-independent phospholipase A2, and Na+/H+ exchange
Am. J. Physiol. Cell Physiol.
296
C1277-C1242
2009
Mus musculus, Mus musculus HL-1
Manually annotated by BRENDA team
Nomura, M.; Takahashi, T.; Uesugi, A.; Tanaka, R.; Kobayashi, S.
Inotodiol, a lanostane triterpenoid, from Inonotus obliquus inhibits cell proliferation through caspase-3-dependent apoptosis
Anticancer Res.
28
2691-2696
2008
Mus musculus
Manually annotated by BRENDA team
Johnke, R.M.; Smith, E.S.; Cariveau, M.J.; Evans, M.J.; Kilburn, J.M.; Bakken, N.T.; Nangami, G.N.
Radioprotection of murine gastrointestinal epithelium by interleukin-1alpha involves down-regulation of the apoptotic response
Anticancer Res.
28
3601-3607
2009
Mus musculus, Mus musculus C57BL/6
Manually annotated by BRENDA team
Yi, J.Y.; Hirabayashi, Y.; Choi, Y.K.; Kodama, Y.; Kanno, J.; Han, J.H.; Inoue, T.; Yoon, B.I.
Benzene activates caspase-4 and -12 at the transcription level, without an association with apoptosis, in mouse bone marrow cells lacking the p53 gene
Arch. Toxicol.
83
795-803
2009
Mus musculus
Manually annotated by BRENDA team
Calafat, M.; Larocca, L.; Roca, V.; Hauk, V.; Pregi, N.; Nesse, A.; Perez Leiros, C.
Vasoactive intestinal peptide inhibits tumor necrosis factor-alpha-induced apoptotic events in acinar cells from non-obese diabetic mice submandibular glands
Arthritis Res. Ther.
11
R53
2009
Mus musculus
Manually annotated by BRENDA team
Jen, C.Y.; Lin, C.Y.; Leu, S.F.; Huang, B.M.
Cordycepin induced MA-10 mouse Leydig tumor cell apoptosis through caspase-9 pathway
Evid. Based. Complement Alternat. Med.
2009
1-10
2009
Mus musculus
Manually annotated by BRENDA team
French, C.J.; Spees, J.L.; Zaman, A.K.; Taatjes, D.J.; Sobel, B.E.
The magnitude and temporal dependence of apoptosis early after myocardial ischemia with or without reperfusion
FASEB J.
23
1177-1185
2009
Mus musculus, Mus musculus C57BL6
Manually annotated by BRENDA team
Bogazzi, F.; Ultimieri, F.; Raggi, F.; Russo, D.; Lombardi, M.; Cosci, C.; Brogioni, S.; Gasperi, M.; Bartalena, L.; Martino, E.
Reduced colonic apoptosis in mice overexpressing bovine growth hormone occurs through changes in several kinase pathways
Growth Horm. IGF Res.
19
432-441
2009
Mus musculus
Manually annotated by BRENDA team
Jin, D.; Ojcius, D.M.; Sun, D.; Dong, H.; Luo, Y.; Mao, Y.; Yan, J.
Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways
Infect. Immun.
77
799-809
2009
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Harikumar, K.B.; Kuttan, G.; Kuttan, R.
Phyllanthus amarus inhibits cell growth and induces apoptosis in Dalton's lymphoma ascites cells through activation of caspase 3 and down regulation of bcl-2
Integr. Cancer Ther.
8
190-194
2009
Mus musculus, Mus musculus BALB/c
Manually annotated by BRENDA team
Barry, G.M.; Breakwell, L.; Fragkoudis, R.; Attarzadeh-Yazdi, G.; Rodriguez-Andres, J.; Kohl, A.; Fazakerley, J.K.
PKR acts early in infection to suppress Semliki Forest virus production and strongly enhances the type-I interferon response
J. Gen. Virol.
90
1382-1391
2009
Mus musculus
Manually annotated by BRENDA team
Caballero, B.; Vega-Naredo, I.; Sierra, V.; Huidobro-Fernandez, C.; Soria-Valles, C.; De Gonzalo-Calvo, D.; Tolivia, D.; Pallas, M.; Camins, A.; Rodriguez-Colunga, M.J.; Coto-Montes, A.
Melatonin alters cell death processes in response to age-related oxidative stress in the brain of senescence-accelerated mice
J. Pineal Res.
46
106-114
2009
Mus musculus
Manually annotated by BRENDA team
Bae, K.H.; Moon, C.W.; Lee, Y.; Park, T.G.
Intracellular delivery of heparin complexed with chitosan-g-poly(ethylene glycol) for inducing apoptosis
Pharm. Res.
26
93-100
2009
Mus musculus
Manually annotated by BRENDA team
Bhutia, S.K.; Mallick, S.K.; Maiti, S.; Maiti, T.K.
Inhibitory effect of Abrus abrin-derived peptide fraction against Daltons lymphoma ascites model
Phytomedicine
16
377-385
2009
Mus musculus
Manually annotated by BRENDA team
Kim, K.W.; Moretti, L.; Lu, B.
M867, a novel selective inhibitor of caspase-3 enhances cell death and extends tumor growth delay in irradiated lung cancer models
PLoS ONE
3
e2275
2008
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Wang, X.H.; Zhang, L.; Mitch, W.E.; LeDoux, J.M.; Hu, J.; Du, J.
Caspase-3 cleaves specific 19 S proteasome subunits in skeletal muscle stimulating proteasome activity
J. Biol. Chem.
285
21249-21257
2010
Mus musculus
Manually annotated by BRENDA team
Katao, Y.; Shishido, M.; Inami, K.; Matsumoto, N.; Sawai, H.
An inhibitory role for caspase-3 at the late stage of RANKL-induced osteoclast differentiation in RAW264 cells and mouse bone marrow macrophages
Cell Biol. Int.
38
723-728
2014
Mus musculus
Manually annotated by BRENDA team
Dick, S.A.; Chang, N.C.; Dumont, N.A.; Bell, R.A.; Putinski, C.; Kawabe, Y.; Litchfield, D.W.; Rudnicki, M.A.; Megeney, L.A.
Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells
Proc. Natl. Acad. Sci. USA
112
E5246-E5252
2015
Mus musculus (P70677)
Manually annotated by BRENDA team