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Information on EC 3.4.22.43 - cathepsin V
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3.4.22.43 cathepsin VSpecify your search results
Word Map
- 3.4.22.43
- cathepsins
-
papain-like
- elastases
-
elastolytic
- legumain
-
l-like
- medicine
- diagnostics
The enzyme appears in viruses and cellular organisms
Synonyms
cathepsin v, ctsl2, stratum corneum thiol protease, cathepsin v/l2, cathepsin-v, cathepsin l2/v, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
cathepsin L2
cathepsin U
CTSL2
CTSV
cathepsin L2
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-
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cathepsin U
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
The recombinant enzyme hydrolyses proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec)
The recombinant enzyme hydrolyses proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec)
endopeptidase
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-
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The recombinant enzyme hydrolyses proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec)
Cathepsin V is a human lysosomal cysteine endopeptidase of family C1, papain family that is maximally active at pH 5.7 and unstable at neutral pH. Compound E-64, leupeptin and chicken cystatin are inhibitors. Human cathepsin V shows expression restricted to thymus, testis, corneal epithelium and some colon and breast carcinomas. Human gene locus: 9q22.2
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
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CAS REGISTRY NUMBER
COMMENTARY
223670-00-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
best substrate
-
-
?
2-aminobenzoyl-EE-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
2-aminobenzoyl-KK-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
2-aminobenzoyl-KLRSIKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLR + SIKQ-N-(2,4-dinitrophenyl)ethylenediamine
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best substrate
-
-
?
2-aminobenzoyl-KLRSXKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLR + SXKQ-N-(2,4-dinitrophenyl)ethylenediamine
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X is varied with diverse amino acids, highest activity with Ile, kinetics, detailed overview
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-
?
2-aminobenzoyl-KLRXSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLR + XSKQ-N-(2,4-dinitrophenyl)ethylenediamine
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X is varied with diverse amino acids, highest activity with Val, kinetics, detailed overview
-
-
?
2-aminobenzoyl-KLXSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-KLX + SSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
X is varied with diverse amino acids, highest activity with Arg, kinetics, detailed overview
-
-
?
2-aminobenzoyl-KPPVVLLPDQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-KPPVV + LLPDQ-N-[2,4-dinitrophenyl]ethylenediamine
-
-
-
-
?
2-aminobenzoyl-KPVSTEQLAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-KPVS + TEQLAQ-N-[2,4-dinitrophenyl]ethylenediamine
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-
-
-
?
2-aminobenzoyl-KXRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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X is varied with diverse amino acids, highest activity with Leu, kinetics, detailed overview
-
-
?
2-aminobenzoyl-LFEKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-LF + EKQ-N-[2,4-dinitrophenyl]ethylenediamine
-
-
-
-
?
2-aminobenzoyl-VLFEKKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-VLF + EKKQ-N-[2,4-dinitrophenyl]ethylenediamine
-
selective substrate for cathepsin V when compared with cathepsins B, L, and S
-
-
?
2-aminobenzoyl-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-VLF + EKKVY + LQ-N-[2,4-dinitrophenyl]ethylenediamine
-
-
-
-
?
2-aminobenzoyl-VLFEKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
2-aminobenzoyl-VLF + EKQ-N-[2,4-dinitrophenyl]ethylenediamine
-
-
-
-
?
2-aminobenzoyl-XLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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X is varied with diverse amino acids, highest activity with Ala, kinetics, detailed overview
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-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Boc-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Cbz-Phe-Arg-7-amido-4-methylcoumarin + H2O
Cbz-Phe-Arg + 7-amino-4-methylcoumarin
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-
-
-
?
kininogen fragments + H2O
?
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kininogenase activity with high and low molecular weight kininogens
Lys-bradykinin is the major product
-
?
N-benzyloxycarbonyl-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Arg + 7-amino-4-methylcoumarin
-
very low activity
-
?
N-benzyloxycarbonyl-L-Arg-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
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very low activity
-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O
Z-Arg-Arg + 4-methoxy-2-nitroaniline
-
-
-
-
?
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg-Arg + 7-amino-4-methylcoumarin
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-
-
?
Z-Leu-Arg-7-amido-4-methylcoumarin + H2O
Z-Leu-Arg + 7-amino-4-methylcoumarin
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-
-
-
?
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
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-
-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
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-
-
?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
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-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
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-
-
?
Elastin + H2O
?
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elastin degradation activity leading to loss of elasticity in atherosclerosis
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-
?
Elastin + H2O
?
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the enzyme exhibits high elastolytic cysteine protease activity
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-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
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-
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?
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
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-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
Cbz-Phe-Arg-AMC, 25 microM used for protease inhibition assay, inhibitory conditions of cathepsin V propeptide analyzed
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-
?
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
mechanisms of interaction between serpins, human cathepsin V and DNA analyzed, kinetic constants for substrate cleavage in the presence and absence of cofactors determined, final concentrations of cathepsin V and ds65mer DNA at 0.1 and 10 nM, final substrate concentrations ranging from 5 to 200 microM in cathepsin V buffer
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?
plasminogen + H2O
?
capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested
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?
plasminogen + H2O
?
4.5 microM human plasminogen and 30 nM of recombinant human cathepsins V used for plasminogen digestion assay, three major products of 60, 50 and 40 kDa obtained, SDS-PAGE
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?
plasminogen + H2O
?
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cathepsin V hydrolyzes plasminogen at Phe94-Glu95, Ser358-Thr359, and Val468-Leu469 generating angiostatin-related fragments
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?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
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-
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
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-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
role of cathepsis V and the endogenous inhibitor cystatin C in adverse extracellular matrix remodeling of stenotic aortic valves analyzed
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?
additional information
?
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the corneal enzyme stays within cells and is not secreted, suggesting that components of the corneal epithelium are likely substrates
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?
additional information
?
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the predominant expression in thymus suggests a role in the immune system, the expression in testes suggests a role in fertilization processes, the expression in cancer cells together with its absence in normal cells suggests a role in tumor processes
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-
?
additional information
?
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the enzyme and inhibitor cystatin M/E are involved in regulation of epidermal differentiation
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-
?
additional information
?
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the enzyme can stimulate H2O2 production, enzyme expression is increased in keratoconus corneas compared to healthy ones, oxidative stress might be involved in the disorder
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-
?
additional information
?
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substrate and cleavage site specificity of activated recombinant enzyme, comparison to cathepsin L, overview
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-
?
additional information
?
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the enzyme prefers hydrophobic residues in its S2-binding pocket, the substrate specificity is similar to cathepsin L, no cleavage of the triple-helical region of native collagen
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-
?
additional information
?
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the human transglutaminase zymogen is no substrate for the enzyme
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?
additional information
?
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direct interaction of cathepsin V with TLR4 and its adaptor protein MyD88
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?
additional information
?
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direct interaction of cathepsin V with TLR4 and its adaptor protein MyD88
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
Elastin + H2O
?
-
elastin degradation activity leading to loss of elasticity in atherosclerosis
-
-
?
plasminogen + H2O
?
capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested
-
-
?
plasminogen + H2O
?
-
cathepsin V hydrolyzes plasminogen at Phe94-Glu95, Ser358-Thr359, and Val468-Leu469 generating angiostatin-related fragments
-
-
?
additional information
?
-
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the corneal enzyme stays within cells and is not secreted, suggesting that components of the corneal epithelium are likely substrates
-
-
?
additional information
?
-
-
the predominant expression in thymus suggests a role in the immune system, the expression in testes suggests a role in fertilization processes, the expression in cancer cells together with its absence in normal cells suggests a role in tumor processes
-
-
?
additional information
?
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the enzyme and inhibitor cystatin M/E are involved in regulation of epidermal differentiation
-
-
?
additional information
?
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the enzyme can stimulate H2O2 production, enzyme expression is increased in keratoconus corneas compared to healthy ones, oxidative stress might be involved in the disorder
-
-
?
additional information
?
-
direct interaction of cathepsin V with TLR4 and its adaptor protein MyD88
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-
?
additional information
?
-
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direct interaction of cathepsin V with TLR4 and its adaptor protein MyD88
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-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(1R)-1-{2-[butyl(dichloro)-l4-tellanyl]phenyl}ethyl methyl ether
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less than 10% residual activity at 0.001 mM
(1R)-1-{2-[dibromo(butyl)-l4-tellanyl]phenyl}ethyl methyl ether
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less than 10% residual activity at 0.001 mM
(1S)-1-{2-[butyl(dichloro)-l4-tellanyl]phenyl}ethyl methyl ether
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less than 10% residual activity at 0.001 mM
(1S)-1-{2-[dibromo(butyl)-l4-tellanyl]phenyl}ethyl methyl ether
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less than 10% residual activity at 0.001 mM
1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9(10H)-one
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-
2,3-dihydro-4,9-dihydroxy-2-(2-hydroxy-propan-2-yl)-11-methoxy-10-methylfuro[3,2-b]acridin-5(10H)-one
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2-[butyl(dichloro)-l4-tellanyl]benzyl methyl ether
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about 10% residual activity at 0.001 mM
2-[dibromo(butyl)-l4-tellanyl]benzyl methyl ether
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less than 10% residual activity at 0.001 mM
3,4-dihydro-3,5,8-trihydroxy-6-methoxy-2,2,7-trimethyl-2H-pyrano[2,3-a]acridin-12(7H)-one
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4-fluoro-N-prop-2-yn-1-yl-N2-[(1S)-2,2,2-trifluoro-1-[4'-(methanesulfonyl)[1,1'-biphenyl]-4-yl]ethyl]-L-leucinamide
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4-methylpiperazine-1-carboxylic acid [1-[(3-benzenesulfonyl-1-phenethylallyl)carbamoyl]-2-phenylethyl]amide
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i.e. APC-3316
chondroitin 4-sulfate
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specific inhibition of elastolytic activity, formation of specific complexes with the enzyme, no inhibition of the activity with Z-Phe-Arg-4-methyl-7-coumaryl amide
cystatin C
endogenous inhibitor of cathepsin V analyzed, expression of cystatin C increased in stenotic valves, cystatin C protein particularly found in areas with infiltrates of inflammatory cells
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fragment p41 of major histocompatibility complex class II-associated invariant chain
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inhibitory to human cathepsin V, cathepsin L, cathepsin K, cathepsin F with Ki values in the low nanomolar range. Ki values are sufficiently low to ensure complex formation at physiological concentrations. Regulation of the proteolytic activity of most of the cysteine cathepsins by the p41 fragment is an important and widespread control mechanism of antigen presentation
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N-(3-bromoprop-2-yn-1-yl)-4-fluoro-N2-[(1S)-2,2,2-trifluoro-1-[4'-(methanesulfonyl)[1,1'-biphenyl]-4-yl]ethyl]-L-leucinamide
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N-(cyanomethyl)-4-fluoro-N2-[(1S)-2,2,2-trifluoro-1-[4'-(methanesulfonyl)[1,1'-biphenyl]-4-yl]ethyl]-L-leucinamide
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N-but-3-yn-2-yl-4-fluoro-N2-[(1S)-2,2,2-trifluoro-1-[4'-(methanesulfonyl)[1,1'-biphenyl]-4-yl]ethyl]-L-leucinamide
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N-[(1,1-dimethylethoxy)carbonyl]-L-tryptophan-2-[[[2-[(2-ethylphenyl)amino]-2-oxoethyl]thio]carbonyl]hydrazide
saquinavir
SQV, the HIV protease inhibitor Inhibits the interaction between cathepsin V and the TLR4-MyD88 receptor complex, also blocks disulfide HMGB1-induced TLR4 activation
serpin
cofactors fine tuning serpin activity in the extracellular milieu analyzed, DNA accelerate the rate at which the model serpin MENT inhibit cathepsin V up to 50-fold, primarily effected via the protease and secondarily by the recruitment of the DNA as a template onto which cathepsin V and MENT are bound, altered substrate turnover and conformational change within the protease observed, enzyme concentration constant at 0.1 nM, serpin concentration varying between 0.5 and 60 nM with a maximum of 5 nM serpin in the presence of DNA
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STO33438
334, a mammalian protease inhibitor, inhibits cathepsin V, also blocks disulfide HMGB1-induced TLR4 activation
cystatin
cystatin inhibition relies on occlusion of the active site rather than the substrate-like behavior of serpins, unaltered by addition of DNA
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cystatin M/E
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cystatin M/E wild-type and mutant N64A show high affinity for cathepsin V, but not cystatin M/E mutant W135A
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cystatin M/E
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regulatory role, two distinct binding sites for cysteine proteases of the C1 peptidase family, the endogenous inhibitor is co-localized with the enzyme in lamellar granules and corneodesmosomes
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cystatin M/E
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the antiprotease activity of cystatin M/E inhibits the activity of cathepsin V
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cystatin M/E
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high-affinity inhibitor of cathepsin V, directly controls the activity of cathepsin V
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N-[(1,1-dimethylethoxy)carbonyl]-L-tryptophan-2-[[[2-[(2-ethylphenyl)amino]-2-oxoethyl]thio]carbonyl]hydrazide
SID, suppresses the activity of cathepsin V and reverses the upregulation of inflammatory cytokines (IL-6, IL-8 and TNF-alpha), adhesion and chemotaxis of leukocytes and vascular inflammation induced by L-homocysteine (L-Hcy) in vivo and in vitro. SID also inhibits the monocyte-endothelial adhesion and neutrophil chemotaxis induced by L-Hcy. SID reverses this increased phosphorylation of ERK1/2 and STAT1, which is similar to the results obtained in vitro
N-[(1,1-dimethylethoxy)carbonyl]-L-tryptophan-2-[[[2-[(2-ethylphenyl)amino]-2-oxoethyl]thio]carbonyl]hydrazide
SID, inhibits the enzyme and also blocks HMGB1-induced TNF-alpha production
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
the recombinant proenzyme is autocatalytically activated in vitro by incubation at pH 4.0 and 30°C
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Alopecia
Evaluation of the CTSL2 Gene as a Candidate Gene For Alopecia X in Pomeranians and Keeshonden.
Arthritis
Polymorphisms in the cathepsin L2 (CTSL2) gene show association with type 1 diabetes and early-onset myasthenia gravis.
Arthritis, Juvenile
Polymorphisms in the cathepsin L2 (CTSL2) gene show association with type 1 diabetes and early-onset myasthenia gravis.
Arthritis, Rheumatoid
Polymorphisms in the cathepsin L2 (CTSL2) gene show association with type 1 diabetes and early-onset myasthenia gravis.
Atherosclerosis
Solution phase synthesis of a combinatorial library of chalcones and flavones as potent cathepsin V inhibitors.
Autoimmune Diseases
Polymorphisms in the cathepsin L2 (CTSL2) gene show association with type 1 diabetes and early-onset myasthenia gravis.
Breast Neoplasms
An exploratory study of host polymorphisms in genes that clinically characterize breast cancer tumors and pretreatment cognitive performance in breast cancer survivors.
Breast Neoplasms
Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer.
Carcinoma
Significance of nuclear cathepsin V in normal thyroid epithelial and carcinoma cells.
Carcinoma, Ductal
Prognostic significance of cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ.
Carcinoma, Hepatocellular
Elevated CTSL2 expression is associated with an adverse prognosis in hepatocellular carcinoma.
Carcinoma, Intraductal, Noninfiltrating
Prognostic significance of cathepsin V (CTSV/CTSL2) in breast ductal carcinoma in situ.
Colorectal Neoplasms
Cathepsin V Mediates the Tazarotene-induced Gene 1-induced Reduction in Invasion in Colorectal Cancer Cells.
Dermatitis, Atopic
The cystatin M / E-controlled pathway of skin barrier formation: expression of its key components in psoriasis and atopic dermatitis.
Endometrial Neoplasms
Expression of cysteine protease cathepsin L is increased in endometrial cancer and correlates with expression of growth regulatory genes.
Goiter
Significance of nuclear cathepsin V in normal thyroid epithelial and carcinoma cells.
Hyperhomocysteinemia
HMGB1 mediates homocysteine-induced endothelial cells pyroptosis via cathepsin V-dependent pathway.
Keratoconus
Increased levels of catalase and cathepsin V/L2 but decreased TIMP-1 in keratoconus corneas: evidence that oxidative stress plays a role in this disorder.
Lung Neoplasms
Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues.
Mucopolysaccharidoses
The abnormal accumulation of heparan sulfate in patients with mucopolysaccharidosis prevents the elastolytic activity of cathepsin V.
Myasthenia Gravis
Cathepsin V is involved in the degradation of invariant chain in human thymus and is overexpressed in myasthenia gravis.
Neoplasm Metastasis
Cathepsin V suppresses GATA3 protein expression in luminal A breast cancer.
Neoplasm Metastasis
Determination of cathepsin V activity and intracellular trafficking by N-glycosylation.
Neoplasms
An exploratory study of host polymorphisms in genes that clinically characterize breast cancer tumors and pretreatment cognitive performance in breast cancer survivors.
Neoplasms
CTSL2 is a pro-apoptotic target of E2F1 and a modulator of histone deacetylase inhibitor and DNA damage-induced apoptosis.
Neoplasms
Determination of cathepsin V activity and intracellular trafficking by N-glycosylation.
Neoplasms
Elevated CTSL2 expression is associated with an adverse prognosis in hepatocellular carcinoma.
Neoplasms
Expression of cysteine protease cathepsin L is increased in endometrial cancer and correlates with expression of growth regulatory genes.
Neoplasms
Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissues.
Neuroblastoma
Human cathepsin V protease participates in the production of enkephalin and NPY neurotransmitters.
Placenta Accreta
Possible Association between Cathepsin V and the Development of Placenta Accreta Spectrum Disorders.
Psoriasis
The cystatin M / E-controlled pathway of skin barrier formation: expression of its key components in psoriasis and atopic dermatitis.
Sarcoidosis, Pulmonary
Endostatin and cathepsin-V in bronchoalveolar lavage fluid of patients with pulmonary sarcoidosis.
Scleroderma, Systemic
Decreased cathepsin V expression due to Fli1 deficiency contributes to the development of dermal fibrosis and proliferative vasculopathy in systemic sclerosis.
Thyroid Cancer, Papillary
Significance of nuclear cathepsin V in normal thyroid epithelial and carcinoma cells.
Vascular Diseases
l-Homocysteine-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinaemia.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0016
2-aminobenzoyl-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.0017
2-aminobenzoyl-EE-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.002
2-aminobenzoyl-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.0008
2-aminobenzoyl-KK-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.0006
2-aminobenzoyl-KLRSIKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.0012
2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.0006
2-aminobenzoyl-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.0044
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C
0.0024
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C
0.0041
Z-Phe-Arg-4-methyl-7-coumaryl amide
-
recombinant enzyme, pH 5.5, 25°C, in presence of 0.3 M NaCl
additional information
additional information
presence of ds65-mer DNA causes a nonsignificant decrease in the Km value for the interaction between cathepsin V and the substrate, values from about 33.1 microM to 27.5 microM measured
-
additional information
additional information
-
presence of ds65-mer DNA causes a nonsignificant decrease in the Km value for the interaction between cathepsin V and the substrate, values from about 33.1 microM to 27.5 microM measured
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4
2-aminobenzoyl-ALRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
1.3
2-aminobenzoyl-EE-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.6
2-aminobenzoyl-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
1.7
2-aminobenzoyl-KK-epsilon-amino-caproic acid-ELKLQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
1.5
2-aminobenzoyl-KLRSIKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
1.3
2-aminobenzoyl-KLRSSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
0.9
2-aminobenzoyl-KLRVSKQ-N-(2,4-dinitrophenyl)ethylenediamine
-
recombinant enzyme, pH 5.5, 37°C
156
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C
19
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C
3.7
Z-Phe-Arg-4-methyl-7-coumaryl amide
-
recombinant enzyme, pH 5.5, 25°C, in presence of 0.3 M NaCl
additional information
additional information
presence of DNA reduces the turnover rate up to 50% indicating that the binding of DNA changes the active site of cathepsin V such that the enzyme interacts with peptide substrates differently
-
additional information
additional information
-
presence of DNA reduces the turnover rate up to 50% indicating that the binding of DNA changes the active site of cathepsin V such that the enzyme interacts with peptide substrates differently
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
496
2-aminobenzoyl-KPPVVLLPDQ-N-[2,4-dinitrophenyl]ethylenediamine
-
at 37°C, in 0.1 M sodium acetate buffer, pH 5.5
903
2-aminobenzoyl-KPVSTEQLAQ-N-[2,4-dinitrophenyl]ethylenediamine
-
at 37°C, in 0.1 M sodium acetate buffer, pH 5.5
4528
2-aminobenzoyl-LFEKQ-N-[2,4-dinitrophenyl]ethylenediamine
-
at 37°C, in 0.1 M sodium acetate buffer, pH 5.5
3055
2-aminobenzoyl-VLFEKKQ-N-[2,4-dinitrophenyl]ethylenediamine
-
at 37°C, in 0.1 M sodium acetate buffer, pH 5.5
6833
2-aminobenzoyl-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine
-
at 37°C, pH 5.5
4277
2-aminobenzoyl-VLFEKQ-N-[2,4-dinitrophenyl]ethylenediamine
-
at 37°C, in 0.1 M sodium acetate buffer, pH 5.5
35000
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C
7900
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin
in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0012
1,3,5-trihydroxy-2,8-bis(3-methylbut-2-enyl)-10-methyl-9-acridone
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.0005
1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9(10H)-one
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.0044
2,3-dihydro-4,9-dihydroxy-2-(2-hydroxy-propan-2-yl)-11-methoxy-10-methylfuro[3,2-b]acridin-5(10H)-one
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.0017
3,4-dihydro-3,5,8-trihydroxy-6-methoxy-2,2,7-trimethyl-2H-pyrano[2,3-a]acridin-12(7H)-one
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.0002
citibrasine
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.001
citrusinine-I
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.0042
citrusinine-II
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.00000008
clitocypin
pH and temperature not specified in the publication
-
0.0000000072
fragment p41 of major histocompatibility complex class II-associated invariant chain
-
pH 6.0, 25°C
-
0.01
glycocitrine-I
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.0011
glycocitrine-IV
-
in 100 mM sodium acetate buffer (pH 5.5) containing 5 mM EDTA and 5 mM dithioerythritol, at 27°C
0.00000069
macrocypin-1
pH and temperature not specified in the publication
-
0.00000025
VBY-825
-
apparent value, in 50 mM MES pH 6.5, 2.5 mM DTT, 2.5 mM EDTA, 100 mM NaCl, 0.01% (w/v) bovine serum albumin, and 10% (v/v) DMSO, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0039
1,3,5-trihydroxy-2,8-bis(3-methylbut-2-enyl)-10-methyl-9-acridone
Homo sapiens
-