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Information on EC 3.4.22.40 - bleomycin hydrolase and Organism(s) Rattus norvegicus and UniProt Accession P70645

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.40 bleomycin hydrolase
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This record set is specific for:
Rattus norvegicus
UNIPROT: P70645 not found.
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
Synonyms
blh, bleomycin hydrolase, blm hydrolase, htlase, homocysteine thiolactonase, aminopeptidase c, hcy-thiolactonase, aminopeptidase h, hydrolase h, bana-hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminopeptidase C
-
-
-
-
aminopeptidase H
-
-
-
-
BANA-hydrolase
-
-
-
-
Bleomycin hydrolase
-
-
-
-
Blh1p
-
-
-
-
BLM hydrolase
-
-
-
-
BMH
-
-
-
-
citrulline aminopeptidase
-
-
Gal6p
-
-
-
-
hydrolase H
-
-
-
-
PEPC
-
-
-
-
additional information
-
the enzyme belongs to the C1B peptidase family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
53096-17-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
amyloid peptide Abeta1-40 + H2O
peptide fragments
show the reaction diagram
-
processing by cleavage at bonds His14-Gln15 and Phe19-Phe20, endopeptidase activity, substrate preparation, overview
product determination by MALDI-TOF mass spectrometry
-
?
amyloid peptide Abeta1-42 + H2O
peptide fragments
show the reaction diagram
-
processing by cleavage at bonds His14-Gln15 and Phe19-Phe20, endopeptidase activity, substrate preparation, overview
product determination by MALDI-TOF mass spectrometry
-
?
amyloid peptide Abeta3-42 + H2O
?
show the reaction diagram
-
processing
-
-
?
bleomycin + H2O
?
show the reaction diagram
bleomycin + H2O
deamido-bleomycin
show the reaction diagram
-
bleomycin A2
-
?
citrulline-beta-naphthylamide + H2O
citrulline + beta-naphthylamine
show the reaction diagram
-
-
-
-
?
deiminated filaggrin + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
bleomycin + H2O
?
show the reaction diagram
deiminated filaggrin + H2O
?
show the reaction diagram
-
neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-hydroxymeruribenzoate
-
-
iodoacetate
-
-
leupeptin
-
weak inhibition
additional information
-
no inhibition by cystatin A and cystatin C
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
binding to nucleic acids, preferably single-stranded DNA or RNA, is required for enzyme activity
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme is most active at neutral pH
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
encoded by Blmh gene; Wistar rats
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
6-week-old rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
extracts, bleomycin hydrolase detection by Western blot
Manually annotated by BRENDA team
detected by immunohistochemistry
Manually annotated by BRENDA team
of 6-week-old rats, tissue amounts of bleomycin hydrolase determined, relative high amounts detected
Manually annotated by BRENDA team
leukocyte-rich cell extracts, detection by Western blot
Manually annotated by BRENDA team
of 6-week-old rats, tissue amounts of bleomycin hydrolase determined, relative high amounts detected
Manually annotated by BRENDA team
of 6-week-old rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
bleomycin hydrolase detected by immunohistochemistry
Manually annotated by BRENDA team
6-week-old female rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
of 6-week-old rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
hemo-lymphocytic lineage cell line, bleomycin hydrolase detection by Western blot
Manually annotated by BRENDA team
of 6-week-old rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
newborn rats, tissue amounts of bleomycin hydrolase determined, high amounts detected
Manually annotated by BRENDA team
of 6-week-old rats, red pulpy region, detected by immunohistochemistry
Manually annotated by BRENDA team
of 6-week-old rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
6-week-old male rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
6-week-old female rats, tissue amounts of bleomycin hydrolase determined
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
a self-compartmentalizing enzyme
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BLMH_RAT
454
0
52323
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
280000
-
gel filtration
300000
-
about
48000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 48000, SDS-PAGE
hexamer
additional information
-
trypsin-mediated peptide mapping, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration, SDS-PAGE
native enzyme 4300fold from newborn rat epidermis by gel filtration, and two steps of anion exchange chromatography
-
native enzyme from epidermis of newborn rats
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sebti, S.M.; Mignano, J.E.; Jani, J.P.; Srimatkandada, S.; Lazo, J.S.
Bleomycin hydrolase: molecular cloning, sequencing, and biochemical studies reveal membership in the cysteine proteinase family
Biochemistry
28
6544-6548
1989
Gallus gallus, Homo sapiens, Lithobates catesbeianus, Mus musculus, Oryctolagus cuniculus, Papio hamadryas, Rattus norvegicus
Manually annotated by BRENDA team
Joshua-Tor, L.; Johnston, S.A.
Bleomycin hydrolase
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press
2
1197-1201
2004
Saccharomyces cerevisiae, Oryctolagus cuniculus, Homo sapiens, Rattus norvegicus, Streptomyces verticillus
-
Manually annotated by BRENDA team
Kajiya, A.; Kaji, H.; Isobe, T.; Takeda, A.
Processing of amyloid beta-peptides by neutral cysteine protease bleomycin hydrolase
Protein Pept. Lett.
13
119-123
2006
Rattus norvegicus
Manually annotated by BRENDA team
Kamata, Y.; Itoh, Y.; Kajiya, A.; Karasawa, S.; Sakatani, C.; Takekoshi, S.; Osamura, R.Y.; Takeda, A.
Quantification of neutral cysteine protease bleomycin hydrolase and its localization in rat tissues
J. Biochem.
141
69-76
2007
Rattus norvegicus (P70645)
Manually annotated by BRENDA team
Kamata, Y.; Taniguchi, A.; Yamamoto, M.; Nomura, J.; Ishihara, K.; Takahara, H.; Hibino, T.; Takeda, A.
Neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids
J. Biol. Chem.
284
12829-12836
2009
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team