Any feedback?
Information on EC 3.4.22.40 - bleomycin hydrolase
for references in articles please use BRENDA:EC3.4.22.40Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.4.22.40 bleomycin hydrolaseSpecify your search results
Word Map
- 3.4.22.40
-
luteinizing
- paraoxonase
- thiolactone
- helveticus
- peplomycin
-
lutropins
- corneocytes
-
homocysteinylation
- beta-naphthylamide
- medicine
-
hcy-thiolactone
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
Synonyms
blh, bleomycin hydrolase, blm hydrolase, htlase, homocysteine thiolactonase, hcy-thiolactonase, aminopeptidase c, aminopeptidase h, hydrolase h, bana-hydrolase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
aminopeptidase C
-
-
-
-
aminopeptidase H
-
-
-
-
BANA-hydrolase
-
-
-
-
BH
BH protein
Bleomycin hydrolase
-
-
-
-
Blh1p
-
-
-
-
BLM hydrolase
-
-
-
-
Blmh
BLMH protein
BMH
citrulline aminopeptidase
Gal6p
Hcy-thiolactonase
homocysteine thiolactonase
HTLase
hydrolase H
-
-
-
-
PEPC
-
-
-
-
additional information
BMH
-
-
-
-
Gal6p
-
-
-
-
Hcy-thiolactonase
-
the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview
Hcy-thiolactonase
-
the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview
homocysteine thiolactonase
-
the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview
homocysteine thiolactonase
-
the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview
HTLase
-
the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview
HTLase
-
the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview
additional information
-
the enzyme belongs to the C1B peptidase family
additional information
-
the enzyme belongs to the C1B peptidase family
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
acts as carboxypeptidase, aminopeptidase and peptide ligase
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
endopeptidase and aminopeptidase activity
-
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
53096-17-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6'-deoxy bleomycin A2 + H2O
6'-deoxy deamido bleomycin A2 + NH3
recombinant enzyme shows rapid and efficient hydrolysis of all bleomycins tested, exhibiting a superior catalytic efficiency for bleomycin B2
-
-
?
6'-deoxy bleomycin Z + H2O
6'-deoxy deamido bleomycin Z + NH3
recombinant enzyme shows rapid and efficient hydrolysis of all bleomycins tested, exhibiting a superior catalytic efficiency for bleomycin B2
-
-
?
amyloid peptide Abeta1-40 + H2O
peptide fragments
-
processing by cleavage at bonds His14-Gln15 and Phe19-Phe20, endopeptidase activity, substrate preparation, overview
product determination by MALDI-TOF mass spectrometry
-
?
amyloid peptide Abeta1-42 + H2O
peptide fragments
-
processing by cleavage at bonds His14-Gln15 and Phe19-Phe20, endopeptidase activity, substrate preparation, overview
product determination by MALDI-TOF mass spectrometry
-
?
bleomycin Z + H2O
deamido bleomycin Z + NH3
recombinant enzyme shows rapid and efficient hydrolysis of all bleomycins tested, exhibiting a superior catalytic efficiency for bleomycin B2
-
-
?
citrulline-4-methylcoumarin 7-amide + H2O
citrulline + 7-amino-4-methylcoumarin
-
-
-
-
?
citrulline-7-amido-4-methylcoumarin + H2O
citrulline + 7-amino-4-methylcoumarin
-
-
-
-
?
huntingtin + H2O
?
-
the enzyme is involved in a specific cleavage step at the N-terminal fragment cp-2 site of huntingtin
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
protease activity of mutant variants determined using substrate concentrations ranging from 0 to 0.125 mM
-
-
?
L-tyrosine-p-nitroanilide + H2O
L-tyrosine + 4-nitroaniline
72% of activity with phenylalanine-betanitroanilide
-
?
phenylalanine-betanaphthylamide + H2O
beta-naphthylamin + phenylalanine
best substrate
-
?
phenylalanine-p-nitroanilide + H2O
p-nitroaniline + phenylalanine
activity comparable to phenylalanine-betanitroanilide
-
?
Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O
Z-Leu-Leu-Glu + 4-nitroaniline
-
no activity in vitro due to requirement of binding to DNA-recognition sites of ztranscriptional activator Gal4p
-
-
?
bleomycin + H2O
?
-
bleomycin is a cytotoxic agent inducing pneumonitis, detoxification reaction
-
-
?
bleomycin + H2O
?
-
detoxification reaction, high enzyme expression level leads to resistance against the drug
-
-
?
bleomycin A2 + H2O
deamido bleomycin A2 + NH3
the enzyme hydrolyzes bleomycins into the biologically inactive deamido bleomycins
-
-
?
bleomycin A2 + H2O
deamido bleomycin A2 + NH3
recombinant enzyme shows rapid and efficient hydrolysis of all bleomycins tested, exhibiting a superior catalytic efficiency for bleomycin B2
-
-
?
bleomycin B2 + H2O
deamido bleomycin B2 + NH3
the enzyme hydrolyzes bleomycins into the biologically inactive deamido bleomycins
-
-
?
bleomycin B2 + H2O
deamido bleomycin B2 + NH3
recombinant enzyme shows rapid and efficient hydrolysis of all bleomycins tested, exhibiting a superior catalytic efficiency for bleomycin B2
-
-
?
citrulline-beta-naphthylamide + H2O
citrulline + beta-naphthylamine
-
-
-
-
?
deiminated filaggrin + H2O
?
-
neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids
-
-
?
deiminated filaggrin + H2O
?
-
recombinant human filaggrin is a component of the cornified cell envelope and the precursor of free amino acids acting as a natural moisturizing factor in the stratum corneum. Profilaggrin is synthesized as a large, extremely insoluble phosphoprotein. Deimination is critical for the degradation of filaggrin into free amino acids, deimination of filaggrin is catalyzed by murine PAD3
-
-
?
deiminated filaggrin + H2O
?
-
neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids
-
-
?
deiminated filaggrin + H2O
?
-
recombinant human filaggrin is a component of the cornified cell envelope and the precursor of free amino acids acting as a natural moisturizing factor in the stratum corneum. Profilaggrin is synthesized as a large, extremely insoluble phosphoprotein. Deimination is critical for the degradation of filaggrin into free amino acids, deimination of filaggrin is catalyzed by murine PAD3
-
-
?
additional information
?
-
-
the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview
-
-
?
additional information
?
-
-
the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview
-
-
?
additional information
?
-
bleomycin hydrolase as an essential component of chemotherapy regimens for disseminated testicular germ-cell cancer analyzed, study on relation of single nucleotide polymorphism (SNP) A1450G of the bleomycin hydrolase gene with survival according to genotype analyzed
-
-
?
additional information
?
-
-
bleomycin hydrolase as an essential component of chemotherapy regimens for disseminated testicular germ-cell cancer analyzed, study on relation of single nucleotide polymorphism (SNP) A1450G of the bleomycin hydrolase gene with survival according to genotype analyzed
-
-
?
additional information
?
-
-
bleomycin hydrolase is a neutral cysteine protease, the enzyme releases various amino acids except Pro from beta-naphthylamide derivatives and hydrolyzed citrulline-beta-naphthylamide most effectively, overview
-
-
?
additional information
?
-
role in generating MHC class I-presented peptides suggested
-
-
?
additional information
?
-
immunohistochemical analysis and behavioral phenotyping of mice with a targeted deletion of the neutral cysteine protease described
-
-
?
additional information
?
-
role of bleomycin hydrolase in antigen presentation and in generation of CD8 T cell response analyzed in bleomycin hydrolase-deficient mice
-
-
?
additional information
?
-
-
bleomycin hydrolase does not hydrolyze D-Hcy-thiolactone
-
-
?
additional information
?
-
-
the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview
-
-
?
additional information
?
-
-
the enzyme is a neutral cysteine protease, processing specificity on amyloid bet-peptides depends on the substrate structure and sequence, overview
-
-
?
additional information
?
-
-
the substrate specificity is controlled by the opening of the barrel structure and the positioning of the enzyme's C-terminus in the active site as opposed to the features of the substrate, mechanism, overview
-
-
?
additional information
?
-
-
bleomycin hydrolase is a neutral cysteine protease, the enzyme releases various amino acids except Pro from beta-naphthylamide derivatives and hydrolyzed citrulline-beta-naphthylamide most effectively, overview
-
-
?
additional information
?
-
-
the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview
-
-
?
additional information
?
-
-
the enzyme expression is regulated by galactose and Gal4p,expression pattrern
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
bleomycin A2 + H2O
deamido bleomycin A2 + NH3
the enzyme hydrolyzes bleomycins into the biologically inactive deamido bleomycins
-
-
?
bleomycin B2 + H2O
deamido bleomycin B2 + NH3
the enzyme hydrolyzes bleomycins into the biologically inactive deamido bleomycins
-
-
?
huntingtin + H2O
?
-
the enzyme is involved in a specific cleavage step at the N-terminal fragment cp-2 site of huntingtin
-
-
?
Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O
Z-Leu-Leu-Glu + 4-nitroaniline
-
no activity in vitro due to requirement of binding to DNA-recognition sites of ztranscriptional activator Gal4p
-
-
?
bleomycin + H2O
?
-
bleomycin is a cytotoxic agent inducing pneumonitis, detoxification reaction
-
-
?
bleomycin + H2O
?
-
detoxification reaction, high enzyme expression level leads to resistance against the drug
-
-
?
deiminated filaggrin + H2O
?
-
neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids
-
-
?
deiminated filaggrin + H2O
?
-
neutral cysteine protease bleomycin hydrolase is essential for the breakdown of deiminated filaggrin into amino acids
-
-
?
additional information
?
-
-
the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview
-
-
?
additional information
?
-
role in generating MHC class I-presented peptides suggested
-
-
?
additional information
?
-
-
bleomycin hydrolase does not hydrolyze D-Hcy-thiolactone
-
-
?
additional information
?
-
-
the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview
-
-
?
additional information
?
-
-
the enzyme expression is regulated by galactose and Gal4p,expression pattrern
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2-chlorophenyl)methyl [(5S)-5-amino-6-oxo-7-(2,3,5,6-tetrafluorophenoxy)heptyl]carbamate
-
(2-chlorophenyl)methyl [(5S,6E)-5-amino-7-(benzenesulfonyl)hept-6-en-1-yl]carbamate
-
(2-chlorophenyl)methyl [(5S,6E)-5-amino-7-(methanesulfonyl)hept-6-en-1-yl]carbamate
-
(3S)-3-amino-7-([[(2-chlorophenyl)methoxy]carbonyl]amino)-2-oxoheptyl 2,6-dimethylbenzoate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
binding to nucleic acids, preferably single-stranded DNA or RNA, is required for enzyme activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Acute Coronary Syndrome
Association of homocysteine thiolactonase activity and PON1 polymorphisms with the severity of acute coronary syndrome.
Adenocarcinoma
Purification of bleomycin hydrolase with a monoclonal antibody and its characterization.
Adenoma
Immunohistochemical detection of prolactin in clinically non-functioning pituitary adenomas.
Alzheimer Disease
Analysis of association between bleomycin hydrolase and apolipoprotein E polymorphism in Alzheimer's disease.
Alzheimer Disease
Association between bleomycin hydrolase and Alzheimer's disease in caucasians.
Alzheimer Disease
Astrogliosis and behavioral changes in mice lacking the neutral cysteine protease bleomycin hydrolase.