Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

Information on EC 3.4.22.40 - bleomycin hydrolase

for references in articles please use BRENDA:EC3.4.22.40

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IUBMB Comments

The molecule is a homohexamer in which the monomers have a papain-like tertiary structure (in peptidase family C1). The active sites are on the walls of a central channel through the molecule, and access of substrate molecules to them is obstructed by this and by the C-terminus of each polypeptide chain . Bleomycin can scarcely be the natural substrate, and there are reports of limited endopeptidase activity. Known from bacteria as well as eukaryotic organisms. Hydrolase H from chicken muscle has many similarities to bleomycin hydrolase, but hydrolyses Ph-CO-Arg-2-naphthylamine as well as aminopeptidase substrates .

The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred

Synonyms
blh, bleomycin hydrolase, blm hydrolase, htlase, homocysteine thiolactonase, hcy-thiolactonase, aminopeptidase c, aminopeptidase h, hydrolase h, bana-hydrolase, more

REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred
show the reaction diagram