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Information on EC 3.4.22.36 - caspase-1 and Organism(s) Spodoptera frugiperda and UniProt Accession P89116

for references in articles please use BRENDA:EC3.4.22.36
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.36 caspase-1
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This record set is specific for:
Spodoptera frugiperda
UNIPROT: P89116 not found.
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Word Map
The taxonomic range for the selected organisms is: Spodoptera frugiperda
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-/-
Synonyms
caspase-1, caspase 1, casp1, effector caspase, procaspase-1, interleukin-1beta-converting enzyme, interleukin-1 beta converting enzyme, interleukin-1beta converting enzyme, csp-1, il-1beta-converting enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C14.001
-
-
-
-
CASP-1
-
-
-
-
ICE
-
-
-
-
IL-1 beta converting enzyme
-
-
-
-
IL-1BC
-
-
-
-
interleukin-1 beta converting enzyme
-
-
-
-
interleukin-1beta-converting enzyme
-
-
-
-
p45
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
122191-40-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nuclear immunophilin FKBP46 + H2O
?
show the reaction diagram
-
-
-
?
p35 + H2O
100000 Da fragment + 25000 Da fragment
show the reaction diagram
cleavage at Asp87
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nuclear immunophilin FKBP46 + H2O
?
show the reaction diagram
-
-
-
?
p35 + H2O
100000 Da fragment + 25000 Da fragment
show the reaction diagram
cleavage at Asp87
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p35
IC50: 0.5 nM
-
carbobenzyloxy-Asp-Glu-Val-Asp-fluoromethyl ketone
-
specific to suppress the activity of Sf-caspase-1 by blocking amplification of Sfcaspase-1
carbobenzyloxy-Val-Ala-Asp-fluoromethyl ketone
-
a broad-spectrum inhibitor of initiator caspase
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
the enzyme and apoptosis in SF-21 cell line are induced by the conditioned medium of the entomopathogenic fungus, Nomuraea rileyi, cell phenotype, overview
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000005
p35
Spodoptera frugiperda
IC50: 0.5 nM
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CASP1_SPOFR
299
0
33527
Swiss-Prot
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
the proenzyme of Sf caspase-1 is 299 amino acids in length and can undergo autocatalytic processing in Escherichia coli to an active enzyme heterocomplex. Autoprocessing occurs at Asp28-, Asp184, and Asp95 to generate the large p19/p18 and the small p12 subunits
proteolytic modification
-
pro-Sf-caspase-1 with a short domain is activated by an apical caspase through proteolytic cleavage, at the caspase-recognition site between the large and small subunits of pro-Sfcaspase-1, when apoptotic signaling is triggered
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ahmad, M.; Srinivasula, S.M.; Wang, L.; Litwack, G.; Fernandes-Alnemric, T.; Alnemri, E.S.
Sppdoptera frugiperda caspase-1, a novel insect death protease that cleaves the nuclear immunophilin FKBP46, is the target of the baculovirus antiapoptotic protein p35
J. Biol. Chem.
272
1421-1424
1997
Spodoptera frugiperda (P89116), Spodoptera frugiperda
Manually annotated by BRENDA team
Tseng, Y.K.; Wu, M.S.; Hou, R.F.
Induction of apoptosis in SF21 cell line by conditioned medium of the entomopathogenic fungus, Nomuraea rileyi, through Sf-caspase-1 signaling pathway
Arch. Insect Biochem. Physiol.
68
206-214
2008
Spodoptera frugiperda
Manually annotated by BRENDA team