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Information on EC 3.4.22.1 - cathepsin B and Organism(s) Bos taurus and UniProt Accession P07688

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.1 cathepsin B
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Select one or more organisms in this record: ?
This record set is specific for:
Bos taurus
UNIPROT: P07688 not found.
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
Hydrolysis of proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg-/- bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides
Synonyms
cathepsin b, cathepsin-b, cysteine cathepsin, devdase, cat b, cathepsin b1, cathb, cath b, smcb1, cath-b, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
APP secretase
-
-
-
-
cathepsin B1
-
-
-
-
cathepsin II
-
-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9047-22-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-dimethylaminonaphthalene-1-sulfonyl-Phe-Arg-Phe(NO2)-Leu + H2O
?
show the reaction diagram
pH 6.4, 37°C, 2 mM cysteine, 1 mM EDTA
-
-
?
Z-Phe-Arg-4-methylcoumarin-7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-IEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-IEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
no hydrolysis at pH 6.0
-
?
4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-D-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-D-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-L-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-L-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-D-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-D-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-L-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-L-Glu + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-LEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-LEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
no hydrolysis at pH 6.0
-
?
4-(4-dimethylaminophenylazo)benzoyl-LRGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-LRGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RIEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RIEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RIIEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RIIEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
no hydrolysis at pH 6.0
-
?
4-(4-dimethylaminophenylazo)benzoyl-RLEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RLEGIE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RLVG-beta-(2-naphthyl)alanine-E-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RLVG-beta-(2-naphthyl)alanine-E + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RLVGFD-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RLVGFD + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RLVGFE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RLVGFE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RLVGFL-alpha-aminoadipic acid-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RLVGFL-alpha-aminoadipic acid + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
4-(4-dimethylaminophenylazo)benzoyl-RLVGWE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid + H2O
4-(4-dimethylaminophenylazo)benzoyl-RLVGWE + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
show the reaction diagram
-
-
-
?
benzoyl-arginine ethyl ester + H2O
benzoyl-arginine + ethanol
show the reaction diagram
-
-
-
?
benzoyl-DL-Arg-beta-naphthylamide + H2O
benzoyl-DL-Arg + beta-naphthylamine
show the reaction diagram
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + p-nitroaniline
show the reaction diagram
-
-
-
?
benzoyl-DL-argininamide + H2O
benzoyl-DL-arginine + NH3
show the reaction diagram
-
-
-
?
benzoyl-L-Arg-p-nitroanilide + H2O
benzoyl-L-Arg + p-nitroaniline
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Lys-4-nitrophenyl ester + H2O
benzyloxycarbonyl-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
beta-lipotropin + H2O
hydrolyzed beta-lipotropin
show the reaction diagram
-
-
-
?
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Collagen + H2O
Hydrolyzed collagen
show the reaction diagram
-
-
-
?
histones + H2O
hydrolyzed histones
show the reaction diagram
-
-
-
?
myristoylated alanine-rich C kinase substrate + H2O
?
show the reaction diagram
-
i.e. MARCKS
-
-
?
myristoylated alanine-rich C kinase substrate + H2O
p40 + ?
show the reaction diagram
-
i.e. MARCKS
-
?
N-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O
N-benzyloxycarbonyl-L-lysine + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
Proteins + H2O
?
show the reaction diagram
trypsinogen + H2O
trypsin + peptide
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
myristoylated alanine-rich C kinase substrate + H2O
?
show the reaction diagram
-
i.e. MARCKS
-
-
?
Proteins + H2O
?
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-(trans-epoxysuccinyl)-L-leucine 4-guanidinobutylamide
addition of E-64 significantly decreases the activities of cathepsin B and caspase 3, and TUNEL-positive cells in heat-shocked in vitro maturated (IVM) bovine cumulus-oocyte complexes. Addition of inhibitor during in vitro maturation under heat shock conditions significantly improves both developmental competence and quality of the produced embryos
N-chloroacetyl-Gly-Leu-OH
-
N-chloroacetyl-Leu-Leu-OH
-
N-chloroacetyl-Leu-Leu-OMe
-
N-chloroacetyl-Lys-Leu-OH
-
N-chloroacetyl-Phe-Leu-OH
-
N-chloroacetyl-Phe-Met-OH
-
N-chloroacetyl-Ser-Leu-OH
-
2,2'-dipyridyl disulfide
-
-
4-hydroxy-2-nonenal
-
0.015 mM, 76% loss of activity, formation of Michael adducts with C29 of A chain and H150 of B chain
antipain
-
-
Benzyloxycarbonyl-Phe-Ala-CHN2
-
-
BzlNH-tES-Ile-Pro-OBzl
-
-
BzlNH-tES-Ile-Pro-OH
-
-
CA074
-
-
CAA0445
-
noncovalent-type, specific
chymostatin
E-64
-
inhibition of cathepsin B greatly improves the developmental competence of bovine oocytes and increases the number of high-quality embryos, overview
E64c
-
-
EtO-tES-Gly-Pro-OH
-
-
EtO-tES-Ile-Ala-OH
-
-
EtO-tES-Ile-Ile-OH
-
-
EtO-tES-Ile-OH
-
-
EtO-tES-Ile-Pro-OH
-
CA-030
Hg2+
-
-
iodoacetamide
-
-
iodoacetic acid
-
-
L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane
-
E-64
leupeptin
-
-
MeO-Gly-Gly-Leu-NH-tES-Leu-Pro-OH
-
NS-134
N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucylamino-3-methylbutane
-
E64c
N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-Ile-Pro
-
CA-074
N-ethylmaleimide
-
no inhibition at 1 mM
N-tosyl-lysyl chloromethylketone
-
-
oxalo-RAPTA
-
ruthenium(II)-arene compound
p-chloromercuribenzoate
-
-
peptidyl diazomethyl ketone derivatives
-
-
-
PrnNH-tES-Ile-Pro-OBzl
-
-
PrnNH-tES-Ile-Pro-OH
-
CA-074
PrnNH-tES-Ile-Pro-OMe
-
-
Proteinase inhibitors
-
from chicken´s egg white
-
RAPTA-BC
-
ruthenium(II)-arene compound
RAPTA-BI
-
ruthenium(II)-arene compound
RAPTA-C
-
i.e. carbo-RAPTA, ruthenium(II)-arene compound
RAPTA-H
-
ruthenium(II)-arene compound
-
RAPTA-Me+C
-
ruthenium(II)-arene compound
RAPTA-NH3
-
ruthenium(II)-arene compound
RAPTA-OH
-
ruthenium(II)-arene compound
RAPTA-pentaOH
-
binding structure from cyrstal structure of the inhibitor bound to cathepsin B, overview
RAPTA-T
-
ruthenium(II)-arene compound
RAPTA-TBMe
-
ruthenium(II)-arene compound
RAPTA-TBOH
-
ruthenium(II)-arene compound
trans-cis-cis-[RuCl2(DMSO)2(2-amino-5-methyl-thiazole)2]
-
i.e. (PMRu52), a ruthenium(II) compound acting as a strong inhibitor of cathepsin B, crystal structure and binding structure analysis, overview
Zn2+
-
-
[(1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)Pd(1,3,5-triaza-7-phosphaadamantane)Cl]
-
-
[(benzyl(methyl)sulfane)Pd(1,3,5-triaza-7-phosphaadamantane)Cl]
-
-
[(N,N-dimethyl-1-phenylmethanamine)Pd(1,3,5-triaza-7-phosphaadamantane)Cl]
-
-
[Pd2((R)-3-isopropyl-1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,1'-bis(diphenylphosphino)ferrocene)Cl2]
-
-
[Pd2((R)-3-isopropyl-1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,2-bis(diphenylphosphino)ethane)Cl2]
-
-
[Pd2((S)-3-isopropyl-1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,2-bis(diphenylphosphino)ethane)Cl2]
-
-
[Pd2(1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,1'-bis(diphenylphosphino)ferrocene)Cl2]
-
-
[Pd2(1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,2-bis(diphenylphosphino)ethane)Cl2]
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-Dimercaptopropanol
-
-
2-mercaptoethanol
-
activates
2-mercaptoethylamine
-
-
cysteine
-
activates
dithiothreitol
EDTA
-
activates
glutathione
-
activates
thiol compounds
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
4-(4-dimethylaminophenylazo)benzoyl-IEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
pH 4.5
0.0028 - 0.0036
4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-D-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0035 - 0.0053
4-(4-dimethylaminophenylazo)benzoyl-L-Arg-L-Leu-L-Arg-Gly-L-Phe-L-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0046 - 0.0078
4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-D-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0037 - 0.0082
4-(4-dimethylaminophenylazo)benzoyl-L-Leu-L-Arg-Gly-L-Phe-L-Glu-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0056
4-(4-dimethylaminophenylazo)benzoyl-LEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
pH 4.5
0.006 - 0.0066
4-(4-dimethylaminophenylazo)benzoyl-LRGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0035
4-(4-dimethylaminophenylazo)benzoyl-R-LVGFE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
pH 5.0
0.0037 - 0.0046
4-(4-dimethylaminophenylazo)benzoyl-RIEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0055
4-(4-dimethylaminophenylazo)benzoyl-RIIEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
pH 4.5
0.0034 - 0.0049
4-(4-dimethylaminophenylazo)benzoyl-RLEGIE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.001 - 0.0038
4-(4-dimethylaminophenylazo)benzoyl-RLVG-beta-(2-naphthyl)alanine-E-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0029 - 0.0059
4-(4-dimethylaminophenylazo)benzoyl-RLVGF-L-alpha-aminoadipic acid-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.003 - 0.0145
4-(4-dimethylaminophenylazo)benzoyl-RLVGFD-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.0022
4-(4-dimethylaminophenylazo)benzoyl-RLVGFE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
pH 6.0
0.0047 - 0.0078
4-(4-dimethylaminophenylazo)benzoyl-RLVGWE-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
0.53
benzoyl-DL-Arg-beta-naphthylamide
-
-
0.114 - 0.125
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075
benzoyl-DL-Arg-beta-naphthylamide
-
-
52 - 75
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.046
BzlNH-tES-Ile-Pro-OBzl
Bos taurus
-
-
0.000029
BzlNH-tES-Ile-Pro-OH
Bos taurus
-
-
0.0153
EtO-tES-Gly-Pro-OH
Bos taurus
-
-
0.000023
EtO-tES-Ile-Ala-OH
Bos taurus
-
-
0.000024
EtO-tES-Ile-Ile-OH
Bos taurus
-
-
0.024
EtO-tES-Ile-OH
Bos taurus
-
-
0.00012
EtO-tES-Ile-Pro-OH
Bos taurus
-
-
0.00004
N-(L-3-trans-carboxyoxirane-2-carbonyl)-L-leucylamino-3-methylbutane
Bos taurus
-
-
0.2
oxalo-RAPTA
Bos taurus
-
above, pH 6.0, 30°C
0.0091
PrnNH-tES-Ile-Pro-OBzl
Bos taurus
-
-
0.000038
PrnNH-tES-Ile-Pro-OH
Bos taurus
-
-
0.068
PrnNH-tES-Ile-Pro-OMe
Bos taurus
-
-
0.005
RAPTA-BC
Bos taurus
-
pH 6.0, 30°C
0.1
RAPTA-BI
Bos taurus
-
above, pH 6.0, 30°C
0.0025
RAPTA-C
Bos taurus
-
pH 6.0, 30°C
0.2
RAPTA-H
Bos taurus
-
above, pH 6.0, 30°C
-
0.0065
RAPTA-Me+C
Bos taurus
-
pH 6.0, 30°C
0.003
RAPTA-NH3
Bos taurus
-
pH 6.0, 30°C
0.0016
RAPTA-OH
Bos taurus
-
pH 6.0, 30°C
0.007
RAPTA-pentaOH
Bos taurus
-
pH 6.0, 30°C
0.0015
RAPTA-T
Bos taurus
-
pH 6.0, 30°C
0.082
RAPTA-TBMe
Bos taurus
-
pH 6.0, 30°C
0.0245
RAPTA-TBOH
Bos taurus
-
pH 6.0, 30°C
0.0055
trans-cis-cis-[RuCl2(DMSO)2(2-amino-5-methyl-thiazole)2]
Bos taurus
-
pH 6.0, 30°C
0.0002
[(1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)Pd(1,3,5-triaza-7-phosphaadamantane)Cl]
Bos taurus
-
pH 6.0, 30°C
0.00015
[(benzyl(methyl)sulfane)Pd(1,3,5-triaza-7-phosphaadamantane)Cl]
Bos taurus
-
pH 6.0, 30°C
0.00007
[(N,N-dimethyl-1-phenylmethanamine)Pd(1,3,5-triaza-7-phosphaadamantane)Cl]
Bos taurus
-
pH 6.0, 30°C
0.000038
[Pd2((R)-3-isopropyl-1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,1'-bis(diphenylphosphino)ferrocene)Cl2]
Bos taurus
-
pH 6.0, 30°C
0.0000095
[Pd2((R)-3-isopropyl-1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,2-bis(diphenylphosphino)ethane)Cl2]
Bos taurus
-
pH 6.0, 30°C
0.000012
[Pd2((S)-3-isopropyl-1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,2-bis(diphenylphosphino)ethane)Cl2]
Bos taurus
-
pH 6.0, 30°C
0.00003
[Pd2(1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,1'-bis(diphenylphosphino)ferrocene)Cl2]
Bos taurus
-
pH 6.0, 30°C
0.000023
[Pd2(1-methyl-5-phenyl-1H-benzo[e][1,4]diazepin-2(3H)-one)2(m-1,2-bis(diphenylphosphino)ethane)Cl2]
Bos taurus
-
pH 6.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56.9
-
pH 6.0, 37°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4 - 5.5
attack from C-terminus
7.4
endoproteolytic attack
3.5
-
collagen as substrate
6.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
present results implicate cathepsin B activity as one of the possible pathways that are responsible for heat shock-induced apoptosis in bovine cumulus-oocyte complexes
malfunction
additional information
-
relation between cathepsin B activity and the quality of in vitro matured cumulus-oocyte complexes, IVM COCs, and denuded oocytes, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CATB_BOVIN
335
0
36661
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23400
-
x * 31000, single-chain form, x * 23400 or x * 25000, heavy chain of double chain form, SDS-PAGE
25000
26000
-
gel filtration
27500
-
gel filtration, SDS-PAGE
31000
-
x * 31000, single-chain form, x * 23400 or x * 25000, heavy chain of double chain form, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 31000, single-chain form, x * 23400 or x * 25000, heavy chain of double chain form, SDS-PAGE
dimer
monomer
-
1*30000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no redox regulation, enzyme is active over a broad redox potential range
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, in complex with inhibitors, in 50 mM citrate buffer (pH 3.5) containing 2.2-2.6 M sodium phosphate
-
in complex with inhibitor CA074
-
in complex with inhibitor E64c
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme stability in cysteine-containing buffers depends on pH-value, half-life of enzyme at pH 7.0 is about 150 sec, at pH 6.0 about 650 sec, activity of enzyme is similar in both conditions
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
peptides containing Ala or Phe at positions 6 are good substrates for the enzyme at both pH 5.0 and 7.4. Those containing Glu, Asp, Lys or Val are not cleaved at all by cathepsin B at pH 7.4 and are poorly hydrolized at pH 5.0. These findings provide new information for the rational design of cathepsin B-activated peptide-containing anticancer drugs
biotechnology
-
inhibition of cathepsin B greatly improves the developmental competence of bovine oocytes and increases the number of high-quality embryos
diagnostics
-
cathepsin B activity can be a useful marker of oocyte quality
drug development
-
cathepsin B is a target for anticancer metallodrugs, inhibition of cathepsin B by antitumor ruthenium(II)-arene compounds, overview
medicine
-
accumulation of undegraded macromolecules in lysosomes after oxidative damage are caused in part by direct protease inactivation by adduct formation with lipid peroxidation products
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mycek, M.J.
Cathepsins
Methods Enzymol.
19
285-315
1970
Bos taurus, Rattus norvegicus
-
Manually annotated by BRENDA team
Spizz, G.; Blackshear, P.J.
Identification and characterization of cathepsin B as the cellular MARCKS cleaving enzyme
J. Biol. Chem.
272
23833-23842
1997
Bos taurus
Manually annotated by BRENDA team
Suhar, A.; Marks, N.
Purification and properties of brain cathepsin B. Evidence for cleavage of pituitary lipotropins
Eur. J. Biochem.
101
23-30
1979
Bos taurus
Manually annotated by BRENDA team
Etherington, D.J.
Bovine spleen cathepsin B1 and collagenolytic cathepsin. A comparative study of the properties of the two enzymes in the degradation of native collagen
Biochem. J.
153
199-209
1976
Bos taurus
Manually annotated by BRENDA team
Otto, K.; Riesenknig, H.
Improved purification of cathepsin B1 and cathepsin B2
Biochim. Biophys. Acta
379
462-475
1975
Bos taurus
Manually annotated by BRENDA team
Watanabe, H.; Green, G.D.J.; Shaw, E.
A comparison of the behavior of chymotrypsin and cathepsin B towards peptidyl diazomethyl ketones
Biochem. Biophys. Res. Commun.
89
1354-1360
1979
Bos taurus
Manually annotated by BRENDA team
Deval, C.; Bechet, D.; Obled, A.; Ferrara, M.
Purification and properties of different isoforms of bovine cathepsin B
Biochem. Cell Biol.
68
822-826
1990
Bos taurus
Manually annotated by BRENDA team
Stachowiak, K.; Tokmina, M.; Karpinska, A.; Sosnowska, R.; Wiczk, W.
Fluorogenic peptide substrates for carboxydipeptidase activity of cathepsin B
Acta Biochim. Pol.
51
81-92
2004
Bos taurus
Manually annotated by BRENDA team
Sentandreu, M.A.; Aubry, L.; Ouali, A.
Purification of bovine cathepsin B: Proteomic characterization of the different forms and production of specific antibodies
Biochem. Cell Biol.
81
317-326
2003
Bos taurus
Manually annotated by BRENDA team
Yamamoto, A.; Tomoo, K.; Matsugi, K.; Hara, T.; In, Y.; Murata, M.; Kitamura, K.; Ishida, T.
Structural basis for development of cathepsin B-specific noncovalent-type inhibitor: crystal structure of cathepsin B-E64c complex
Biochim. Biophys. Acta
1597
244-251
2002
Bos taurus
Manually annotated by BRENDA team
Pillay, C.S.; Dennison, C.
Cathepsin B stability, but not activity, is affected in cysteine:cystine redox buffers
Biol. Chem.
383
1199-1204
2002
Bos taurus
Manually annotated by BRENDA team
Yamamoto, A.; Tomoo, K.; Hara, T.; Murata, M.; Kitamura, K.; Ishida, T.
Substrate specificity of bovine cathepsin B and its inhibition by CA074, based on crystal structure refinement of the complex
J. Biochem.
127
635-643
2000
Bos taurus
Manually annotated by BRENDA team
Crabb, J.W.; O'Neil, J.; Miyagi, M.; West, K.; Hoff, H.F.
Hydroxynonenal inactivates cathepsin B by forming Michael adducts with active site residues
Protein Sci.
11
831-840
2002
Bos taurus
Manually annotated by BRENDA team
Gilmore, B.F.; Harriott, P.; Walker, B.
The inactivation of bovine cathepsin B by novel N-chloro-acetyl-dipeptides: application of the Houghten tea bag methodology to inhibitor synthesis
Biochem. Biophys. Res. Commun.
333
1284-1288
2005
Bos taurus (P07688), Bos taurus
Manually annotated by BRENDA team
Ruzza, P.; Quintieri, L.; Osler, A.; Calderan, A.; Biondi, B.; Floreani, M.; Guiotto, A.; Borin, G.
Fluorescent, internally quenched, peptides for exploring the pH-dependent substrate specificity of cathepsin B
J. Pept. Sci.
12
455-461
2006
Bos taurus (P07688)
Manually annotated by BRENDA team
Watanabe, D.; Yamamoto, A.; Tomoo, K.; Matsumoto, K.; Murata, M.; Kitamura, K.; Ishida, T.
Quantitative evaluation of each catalytic subsite of cathepsin B for inhibitory activity based on inhibitory activity-binding mode relationship of epoxysuccinyl inhibitors by X-ray crystal structure analyses of complexes
J. Mol. Biol.
362
979-993
2006
Bos taurus
Manually annotated by BRENDA team
Zhou, Z.; Wang, Y.; Bryant, S.H.
Computational analysis of the cathepsin B inhibitors activities through LR-MMPBSA binding affinity calculation based on docked complex
J. Comput. Chem.
30
2165-2175
2009
Bos taurus
Manually annotated by BRENDA team
Casini, A.; Gabbiani, C.; Sorrentino, F.; Rigobello, M.P.; Bindoli, A.; Geldbach, T.J.; Marrone, A.; Re, N.; Hartinger, C.G.; Dyson, P.J.; Messori, L.
Emerging protein targets for anticancer metallodrugs: inhibition of thioredoxin reductase and cathepsin B by antitumor ruthenium(II)-arene compounds
J. Med. Chem.
51
6773-6781
2008
Bos taurus
Manually annotated by BRENDA team
Hoang, l.e..T.V.; Kim, M.M.; Kim, S.K.
Effect of culture conditions on cathepsin B inhibitor production by a marine bacterium, Pseudomonas sp. strain PB01
J. Microbiol. Biotechnol.
18
1115-1120
2008
Bos taurus
Manually annotated by BRENDA team
Spencer, J.; Casini, A.; Zava, O.; Rathnam, R.P.; Velhanda, S.K.; Pfeffer, M.; Callear, S.K.; Hursthouse, M.B.; Dyson, P.J.
Excellent correlation between cathepsin B inhibition and cytotoxicity for a series of palladacycles
Dalton Trans.
2009
10731-10735
2009
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Mura, P.; Camalli, M.; Casini, A.; Gabbiani, C.; Messori, L.
Trans-cis-cis-[RuCl2(DMSO)2(2-amino-5-methyl-thiazole)2], (PMRu52), a novel ruthenium(II) compound acting as a strong inhibitor of cathepsin B
J. Inorg. Biochem.
104
111-117
2010
Bos taurus
Manually annotated by BRENDA team
Balboula, A.Z.; Yamanaka, K.; Sakatani, M.; Hegab, A.O.; Zaabel, S.M.; Takahashi, M.
Cathepsin B activity is related to the quality of bovine cumulus oocyte complexes and its inhibition can improve their developmental competence
Mol. Reprod. Dev.
77
439-448
2010
Bos taurus
Manually annotated by BRENDA team
Balboula, A.Z.; Yamanaka, K.; Sakatani, M.; Kawahara, M.; Hegab, A.O.; Zaabel, S.M.; Takahashi, M.
Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus-oocyte complexes exposed to heat shock during in vitro maturation
Reproduction
146
407-417
2013
Bos taurus (P07688), Bos taurus
Manually annotated by BRENDA team