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Information on EC 3.4.21.B5 - mast cell protease 5 and Organism(s) Rattus norvegicus and UniProt Accession P50339

for references in articles please use BRENDA:EC3.4.21.B5
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.B5 mast cell protease 5
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This record set is specific for:
Rattus norvegicus
UNIPROT: P50339
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
endoprotease activity
Synonyms
mmcp-5, rmcp-5, mast cell protease-5, mast cell protease 5, rat mast cell protease 5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-chymase
-
rat mast cell protease 5
-
mast cell protease-5
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
cleavage of C-N-linkage
-
CAS REGISTRY NUMBER
COMMENTARY hide
142539-77-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Protein + H2O
?
show the reaction diagram
-
-
?
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide + H2O
?
show the reaction diagram
highest activity
-
?
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide + H2O
?
show the reaction diagram
-
-
?
succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
?
show the reaction diagram
-
-
?
succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
?
show the reaction diagram
low activity
-
?
succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
?
show the reaction diagram
highest activity
-
?
additional information
?
-
-
mast cell protease-5 is involved in sprouting and advance of new blood vessels
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Protein + H2O
?
show the reaction diagram
-
-
?
additional information
?
-
-
mast cell protease-5 is involved in sprouting and advance of new blood vessels
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-1-antitrypsin
forms a covalent complex with the enzyme, heparin protects complex formation
-
diisopropyl fluorophosphates
binds covalently to the enzyme
additional information
-
the CB1 and CB2 receptor agonists arachidonyl-20-chloroethylamide and 2-methyl-(1-propyl-1H-indol-3-yl)-1-naphthalenylmethanone (0.001-0.1 mg/ml) prevent the transcription and expression of mast cell protease-5
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.6
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide
pH 8.5
3.1
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide
pH 8.5
4
succinyl-Ala-Ala-Pro-Val-4-nitroanilide
pH 8.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide
pH 8.5
0.0072
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide
pH 8.5
0.017
succinyl-Ala-Ala-Pro-Val-4-nitroanilide
pH 8.5
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CMA1_RAT
247
0
27569
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25390
calculated from amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
1% (w/v) lambda-carrageenan treatment results in accumulation of mast cell protease-5 transcript compared to saline
-
the administration of palmitoylethanolamide (200, 400, and 800 mg/ml) significantly decreases mast cell protease-5 mRNA expression
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Karlson, U.; Pejler, G.; Tomasini-Johansson, B.; Hellman, L.
Extended substrate specificity of rat mast cell protease 5, a rodent alpha-chymase with elastase-like primary specificity
J. Biol. Chem.
278
39625-39631
2003
Rattus norvegicus (P50339)
Manually annotated by BRENDA team
De Filippis, D.; Russo, A.; DAmico, A.; Esposito, G.; Pietropaolo, C.; Concetta, P.; Cinelli, M.; Russo, G.; Iuvone, T.
Cannabinoids reduce granuloma-associated angiogenesis in rats by controlling transcription and expression of mast cell protease-5
Br. J. Pharmacol.
154
1672-1679
2008
Rattus norvegicus
Manually annotated by BRENDA team
De Filippis, D.; Russo, A.; De Stefano, D.; Cipriano, M.; Esposito, D.; Grassia, G.; Carnuccio, R.; Russo, G.; Iuvone, T.
Palmitoylethanolamide inhibits rMCP-5 expression by regulating MITF activation in rat chronic granulomatous inflammation
Eur. J. Pharmacol.
725
64-69
2014
Rattus norvegicus
Manually annotated by BRENDA team