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Information on EC 3.4.21.B42 - hippostasin and Organism(s) Homo sapiens and UniProt Accession Q9UBX7
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3.4.21.B42 hippostasinSpecify your search results
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- 3.4.21.B42
- kallikreins
- klk8
-
prostate-specific
- medicine
- soman
- diagnostics
-
immunofluorometric
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
klk11, hippostasin, kallikrein-like protease, gk-11, human kallikrein 11, kallikrein 11, prostate-type klk11, tissue kallikrein 11, kallikrein-related peptidase 11, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kallikrein 11
S01.257
-
-
-
-
kallikrein 11
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
proteolytic cleavage of polypeptides, preferential cleavage site: Arg-
enzyme is trypsin-like a serine protease
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
hydrolysis of peptide bond
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
342900-22-5
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9001-01-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
acetyl-Arg-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
Arg-4-methyl-coumaryl-7-amide + H2O
Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
benzyl-Arg-4-methyl-coumaryl-7-amide + H2O
benzyl-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
benzyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
benzyl-Phe-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
benzyloxycarbonyl-Gln-Ala-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Gln-Ala-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
benzyloxycarbonyl-Val-Leu-Lys-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
?
insulin-like growth factor-binding protein 3 + H2O
?
insulin-like growth factor-binding protein 3 is cleaved into 3 fragments of 25000, 20000 and 17000 Da length, respectively
-
-
?
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Pro-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
-
-
?
Pyr-Gly-Arg-4-methyl-coumaryl-7-amide + H2O
Pyr-Gly-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
Val-Pro-Arg + 7-amino-4-methylcoumarin
-
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
best substrate
-
-
?
acetyl-Phe-Arg-4-methyl-coumaryl-7-amide + H2O
acetyl-Phe-Arg + 7-amino-4-methylcoumarin
recombinant enzyme
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.27
Gln-Ala-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
1.18
Gly-Pro-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
0.44
Phe-Ser-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
0.53
Pro-Phe-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
0.28
Val-Pro-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 7.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.23
Gln-Ala-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
0.21
Gly-Pro-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
0.39
Phe-Ser-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
1.86
Pro-Phe-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
0.45
Val-Pro-Arg-7-amido-4-methylcoumarin
20 mM Tris, 150 mM NaCl, 0.01% Tween 20 (pH7.5), 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
KLK11 is upregulated by estrogens, dexamethasone, androgens and progestins in breast cancer cell lines
KLK11 is upregulated by estrogens in cervical cancer cell lines but downregulated by dexamethasone
patients with a high enzyme activity have a longer longer overall survival and progression free survival, expression analysis in 138 cancer samples and 40 healthy controls
overexpression of human kallikrein 11 is associated with poor prognosis in patients with low rectal carcinoma
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decreased mRNA level compared to nonmalignant lung tissue
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decreased mRNA level compared to nonmalignant lung tissue
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gene expression, no significant difference to non-cancerous tissue
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higher levels of kallikrein 11 compared to non-cancerous cells
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to homogeneity by immunoaffinity chromatography and high-performance liquid chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection
gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection
molecular cloning of four novel splice variants of the human KLK11 gene, discovered by combining 3' rapid amplification of cDNA ends (3' RACE), NGS technology, advanced bioinformatic analysis, and Sanger sequencing
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
both isoforms of KLK11 (prostate-type and brain-type) are significantly higher in cancer tissues compared with their normal counterparts
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kallikrein 11 expression is significantly decreased in gastric cancer compared with that in normal gastric mucosa
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kallikrein-related peptidase 11 expression is decreased in prostate cancer cells compared to normal/benign prostate cells
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regarding prostate-type KLK11, there is a significant association between lower expression and higher tumor stage
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
diagnostics
medicine
overexpression of human kallikrein 11 is associated with poor prognosis in patients with low rectal carcinoma
medicine
diagnostics
the measurement of kallikrein-related peptidase 11, KLK11, might be a useful diagnostic and prognostic test for non-small cell lung cancer patients. Patients with a high enzyme activity have a longer longer overall survival and progression free survival
diagnostics
elevated tumor tissue protein expression levels of kallikrein-related peptidases KLK10 and KLK11 are associated with a favorable prognosis in advanced high-grade serous ovarian cancer patients
medicine
-
KLK11 mRNA may serve as a marker to discriminate between benign prostatic hyperplasia and prostate cancer
medicine
-
the down-regulation of KLK11 gene in advanced and more aggressive tumors opens the feasibility of being used as biomarker distinguishing the tumor aggressiveness and prognostic indicators for human prostate cancer
medicine
-
negative kallikrein 11 expression is associated with nearly 5fold increased risk of distant metastasis after curative gastrectomy in gastric cancer patients. Kallikrein 11 expression is a significant independent prognostic factor for disease-free survival and overall survival in gastric cancer patients
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mitsui, S.; Yamada, T.; Okui, A.; Kominami, K.; Uemura, H.; Yamaguchi, N.
A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate
Biochem. Biophys. Res. Commun.
272
205-211
2000
Homo sapiens (Q9UBX7), Homo sapiens
Petraki, C.D.; Papanastasiou, P.A.; Karavana, V.N.; Diamandis, E.P.
Cellular distribution of human tissue kallikreins: immunohistochemical localization
Biol. Chem.
387
653-663
2006
Homo sapiens
Planque, C.; Ainciburu, M.; Heuze-Vourch, N.; Regina, S.; de Monte, M.; Courty, Y.
Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
Biol. Chem.
387
783-788
2006
Homo sapiens
Scorilas, A.; Gregorakis, A.K.
mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
Biol. Chem.
387
789-793
2006
Homo sapiens
Diamandis, E.P.; Borgono, C.A.; Scorilas, A.; Harbeck, N.; Dorn, J.; Schmitt, M.
Human kallikrein 11: an indicator of favorable prognosis in ovarian cancer patients
Clin. Biochem.
37
823-829
2004
Homo sapiens
Luo, L.Y.; Shan, S.J.; Elliott, M.B.; Soosaipillai, A.; Diamandis, E.P.
Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma
Clin. Cancer Res.
12
742-750
2006
Homo sapiens (Q9UBX7), Homo sapiens
Stavropoulou, P.; Gregorakis, A.K.; Plebani, M.; Scorilas, A.
Expression analysis and prognostic significance of human kallikrein 11 in prostate cancer
Clin. Chim. Acta
357
190-195
2005
Homo sapiens
Sasaki, H.; Kawano, O.; Endo, K.; Suzuki, E.; Haneda, H.; Yukiue, H.; Kobayashi, Y.; Yano, M.; Fujii, Y.
Decreased kallikrein 11 messenger RNA expression in lung cancer
Clin. Lung Cancer
8
45-48
2006
Homo sapiens
Stephan, C.; Meyer, H.A.; Cammann, H.; Nakamura, T.; Diamandis, E.P.; Jung, K.
Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
Biol. Chem.
387
801-805
2006
Homo sapiens (Q9UBX7), Homo sapiens
McIntosh, M.W.; Liu, Y.; Drescher, C.; Urban, N.; Diamandis, E.P.
Validation and characterization of human kallikrein 11 as a serum marker for diagnosis of ovarian carcinoma
Clin. Cancer Res.
13
4422-4428
2007
Homo sapiens
Mitsui, S.; Nakamura, T.; Okui, A.; Kominami, K.; Uemura, H.; Yamaguchi, N.
Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11/hippostasin
FEBS J.
273
3678-3686
2006
Homo sapiens (Q9UBX7), Homo sapiens
Sano, A.; Sangai, T.; Maeda, H.; Nakamura, M.; Hasebe, T.; Ochiai, A.
Kallikrein 11 expressed in human breast cancer cells releases insulin-like growth factor through degradation of IGFBP-3
Int. J. Oncol.
30
1493-1498
2007
Homo sapiens (Q9UBX7), Homo sapiens
Shaw, J.L.; Diamandis, E.P.
Regulation of human tissue kallikrein-related peptidase expression by steroid hormones in 32 cell lines
Biol. Chem.
389
1409-1419
2008
Homo sapiens (Q9UBX7)
Yu, X.; Tang, H.Y.; Li, X.R.; He, X.W.; Xiang, K.M.
Over-expression of human kallikrein 11 is associated with poor prognosis in patients with low rectal carcinoma
Med. Oncol.
27
40-44
2010
Homo sapiens (Q9UBX7), Homo sapiens
Bi, X.; He, H.; Ye, Y.; Dai, Q.; Han, Z.; Liang, Y.; Zhong, W.
Association of TMPRSS2 and KLK11 gene expression levels with clinical progression of human prostate cancer
Med. Oncol.
27
145-151
2010
Homo sapiens
Wen, Y.G.; Wang, Q.; Zhou, C.Z.; Yan, D.W.; Qiu, G.Q.; Yang, C.; Tang, H.M.; Peng, Z.H.
Identification and validation of Kallikrein-ralated peptidase 11 as a novel prognostic marker of gastric cancer based on immunohistochemistry
J. Surg. Oncol.
104
516-524
2011
Homo sapiens
Jamaspishvili, T.; Scorilas, A.; Kral, M.; Khomeriki, I.; Kurfurstova, D.; Kolar, Z.; Bouchal, J.
Immunohistochemical localization and analysis of kallikrein-related peptidase 7 and 11 expression in paired cancer and benign foci in prostate cancer patients
Neoplasma
58
298-303
2011
Homo sapiens
Xu, C.H.; Zhang, Y.; Yu, L.K.
The diagnostic and prognostic value of serum human kallikrein-related peptidases 11 in non-small cell lung cancer
Tumour Biol.
35
5199-5203
2014
Homo sapiens (Q9UBX7), Homo sapiens
Geng, X.; Liu, Y.; Dreyer, T.; Bronger, H.; Drecoll, E.; Magdolen, V.; Dorn, J.
Elevated tumor tissue protein expression levels of kallikrein-related peptidases KLK10 and KLK11 are associated with a better prognosis in advanced high-grade serous ovarian cancer patients
Am. J. Cancer Res.
8
1856-1864
2018
Homo sapiens (Q9UBX7), Homo sapiens
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