3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme
3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions; factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
mature enzyme is composed of an amino-terminal clip-like domain and a carboxyl-terminal serine protease domain homologous to that of human plasma prekallikrein
sequence contains a prepropeptide of 23 amino acids, that may be processed by both a signal peptidase and a processing protease. Cleavage sites of the zymogen for activation by limulus active factor C are an Arg-Ser and an Ile-Ile bond