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3 acetyl-Pro-Ala-Phe-Phe-NH2 + H2O
acetyl-Pro-Ala-Phe-Phe + NH3 + acetyl-Pro-Ala + Phe-Phe-NH2 + acetyl-Pro-Ala-Phe + Phe-NH2
-
hydrolyzed at the Ala-Phe, the Phe-Phe and the Phe-NH2 bond
-
-
?
acetyl-Ala-Ala-Tyr-4-methylcoumarin 7-amide + H2O
acetyl-Ala-Ala-Tyr + 7-amino-4-methylcoumarin
-
-
-
-
?
Acetyl-Pro-Ala-Ala-Phe-NH2 + H2O
?
-
split at the amide bond as well as at the Ala-Ala bond
-
-
?
Acetyl-Pro-Ala-Gly-Phe-NH2 + H2O
?
-
split at the amide bond as well as at the Ala-Gly bond
-
-
?
Acetyl-Pro-Ala-Leu-Phe-NH2 + H2O
Acetyl-Pro-Ala-Leu-Phe-OH + NH3
-
hydrolyzed at the amide bond only
-
-
?
Acetyl-Pro-Ala-Pro-Phe-NH2 + H2O
Acetyl-Pro-Ala-Pro-Phe-OH + NH3
-
hydrolyzed at the amide bond only
-
-
?
N-Acetyl-L-Pro-L-Leu-p-nitroanilide + H2O
?
-
-
-
-
?
OMSVP3 + H2O
?
-
ovomucoid third domain from silver pheasant, potent inhibitor of chymotrypsin, subtilisin, and elastase
cleavage between residues Met18-Glu19
-
?
OMSVP3 mutant P14C/N39C + H2O
?
-
disulfide variant of the ovomucoid third domain from silver pheasant carrying an engineered Cys14-Cys39 bond near the reactive site
cleavage between residues Met18-Glu19. P14C/N39C can be selectively cleaved by Streptomyces griseus protease B at the reactive site of OMSVP3 to form a reactive site modified inhibitor. The conversion rate of P14C/N39C is much faster than that for wild type under any pH condition. The reactive site modified form of P14C/N39C is thermodynamically more stable than the intact one
-
?
Sc-AAPF-p-nitroanilide + H2O
?
-
-
-
-
?
Sc-AAPF-SBn + FAASF-NH2
?
-
-
ligation to nonapeptide by mutant S195A/T213L/F228H
-
?
Sc-AAPF-SBn + H2O
?
-
-
-
-
?
Suc-AAPF-p-nitroanilide + H2O
?
-
-
-
?
Suc-AAPF-SBzl + H2O
Suc-AAPF + phenylmethanethiol
-
-
-
?
succinyl-Ala-Ala-Pro-4-methylcoumarin 7-amide + H2O
succinyl-Ala-Ala-Pro + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O
succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
-
-
-
-
?
succinyl-Ala-Ala-Pro-X-4-nitroanilide + H2O
?
-
-
-
?
succinyl-Ala-Ala-Pro-X-7-amido-4-methylcoumarin + H2O
?
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Asp-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Asp + 4-nitroaniline
-
extremely poor substrate at pH 8 but becomes a moderately good substrate at pH 5.5
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Leu + 4-nitroaniline
-
optimum at pH 7.5-8.0
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
-
optimum at pH 10.0
-
-
?
additional information
?
-
additional information
?
-
-
interacts favorably with peptides with hydrophobic non-aromatic, P2 amino acid residues, leucine being optimal
-
-
?
additional information
?
-
-
the catalytic preference of protease B for Phe, Tyr, Trp and leucyl-glycine peptide bonds under denaturing conditions enhances its utility in the site-specific proteolysis of insoluble or otherwise proteolysis-resistant polypeptide substrates
-
-
?
additional information
?
-
-
member of the chymotrypsin superfamily of serine proteases
-
?
additional information
?
-
-
member of the chymotrypsin superfamily of serine proteases
-
?
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Bovine pancreatic trypsin inhibitor
-
Kunitz
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N-tert-Butyloxycarbonyl-glycyl-L-leucyl-L-phenylalanine chloromethyl ketone
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-
N-tert-Butyloxycarbonyl-L-alanylglycyl-L-phenylalanine chloromethyl ketone
-
-
protein inhibitor S13D14Y15-OMTKY3
a OMTKY3 mutant variant, recombinantly produced in Escherichia coli strain RV308 and purified, strong inhibition, overview
-
protein inhibitor S13D14Y15G18I19K21-OMTKY3
a OMTKY3 mutant variant, recombinantly produced in Escherichia coli strain RV308 and purified, strong inhibition, overview. S13D14Y15G18I19K21 OMTKY3 inhibits Streptomyces griseus protease B 10times more strongly than Streptomyces griseus protease A, EC 3.4.21.80
-
Soybean trypsin inhibitor
-
-
-
Streptomyces subtilisin inhibitor
-
-
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Third domain of the ovomucoid inhibitor from turkey
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-
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Turkey ovomucoid third domain
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OMTKY3, inhibitor for serine peptidases
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additional information
-
design of inhibitors based on turkey ovomucoid third domain OMTKY3. Variant 13D14Y15G18I19K21-OMTKY3 displays a Gly residue in position P1 and represents the strongest possible inhibitor due to consensus contacts to the enzyme. Inhibitor has significantly higher DeltaGo value for protease B than that for protease A and inhibits protease B 10 times more strongly than protease A
-
additional information
design of inhibitors based on turkey ovomucoid third domain OMTKY3. Variant 13D14Y15G18I19K21-OMTKY3 displays a Gly residue in position P1 and represents the strongest possible inhibitor due to consensus contacts to the enzyme. Inhibitor has significantly higher DeltaGo value for protease B than that for protease A and inhibits protease B 10 times more strongly than protease A
-
additional information
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the enzyme is active in presence of guanidinium chloride
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additional information
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OMTKY3 inhibitor variant screening, overview
-
additional information
OMTKY3 inhibitor variant screening, overview
-
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Henderson, G.; Krygsman, P.; Liu, C.J.; Davey, C.C.; Malek, L.T.
Characterization and structure of genes for proteases A and B from Streptomyces griseus
J. Bacteriol.
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3778-3784
1987
Streptomyces griseus
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Bauer, C.A.
Active centers of alpha-chymotrypsin and of Streptomyces griseus proteases 1 and 3. S2-P2 enzyme-substrate interactions
Eur. J. Biochem.
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1980
Streptomyces griseus
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Interactions of Streptomyces subtilisin inhibitor with Streptomyces griseus proteases A and B. Enzyme kinetic and computer simulation studies
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Greenblatt, H.M.; Ryan, C.A.; James, M.N.G.
Structure of the complex of Streptomyces griseus proteinase B and polypeptide chymotrypsin inhibitor-1 from Russet Burbank potato tubers at 2.1 A resolution
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201-228
1989
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Jurasek, L.; Fackre, D.; Smillie, L.B.
Remarkable homology about the disulfide bridges of a trypsin-like enzyme from Streptomyces griseus
Biochem. Biophys. Res. Commun.
37
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1969
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Fujinaga, M.; Read, R.J.; Sielecki, A.; Ardelt, W.; Laskowski, M.; James, M.N.G.
Refined crystal structure of the molecular complex of Streptomyces griseus protease B, a serine protease, with the third domain of the ovomucoid inhibitor from turkey
Proc. Natl. Acad. Sci. USA
79
4868-4872
1982
Streptomyces griseus
brenda
Read, R.J.; Fujinaga, M.; Sielecki, A.R.; James, M.N.G.
Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8-A resolution
Biochemistry
22
4420-4433
1983
Streptomyces griseus
brenda
Jurasek, L.; Carpenter, M.R.; Smillie, L.B.; Gertler, A.; Levy, S.; Ericsson, L.H.
Amino acid sequence of Streptomyces griseus protease B, A MAJOR COMPONENT OF Pronase
Biochem. Biophys. Res. Commun.
61
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1974
Streptomyces griseus
brenda
Otlewski, J.; Zbyryt, T.; Krokoszynska, I.; Wilusz, T.
Inhibition of serine proteinases by squash inhibitors
Biol. Chem. Hoppe-Seyler
371
589-594
1990
Streptomyces griseus
brenda
Delbaere, D.T.J.; Brayer, G.D.; James, M.N.G.
The 2.8 A resolution structure of Streptomyces griseus protease B and its homology with alpha-chymotrypsin and Streptomyces griseus protease A
Can. J. Biochem.
57
135-144
1979
Streptomyces griseus
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James, M.N.G.; Brayer, G.D.; Delbaere, L.T.J.; Sielecki, A.R.
Crystal structure studies and inhibition kinetics of tripeptide chloromethyl ketone inhibitors with Streptomyces griseus protease B
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139
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1980
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Huang, K.; Wuyuan, L.; Anderson, S.; Laskowski, M.; James, M.N.G.
Water molecules participate in proteinase-inhibitor interactions: crystal structures of Leu18, Ala18, and Gly18 variants of turkey ovomucoid inhibitor third domain complexed with Streptomyces griseus proteinase B
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4
1985-1997
1995
Streptomyces griseus
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Johnson, E.R.; Lamkin, R.M.; Jordan, K.J.; Gordon, J.A.; Lindley, B.R.
Determination of the cleavage specificity of Streptomyces griseus protease B in the presence of guanidinium chloride
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30
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1987
Streptomyces griseus
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Bauer, C.A.
Purity and pH-dependence of Streptomyces griseus protease 1
Acta Chem. Scand. B
31
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1977
Streptomyces griseus
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Elliott, R.J.; Bennet, A.J.; Braun, C.A.; MacLeod, A.M.; Borgford, T.J.
Active-site variants of Streptomyces griseus protease B with peptide-ligation activity
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7
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2000
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Baardsnes, J.; Sidhu, S.; MacLeod, A.; Elliott, J.; Morden, D.; Watson, J.; Borgford, T.
Streptomyces griseus protease B: secretion correlates with the length of the propeptide
J. Bacteriol.
180
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brenda
Bateman, K.S.; Huang, K.; Anderson, S.; Lu, W.; Qasim, M.A.; Laskowski, M.Jr.; James, M.N.
Contribution of peptide bonds to inhibitor-protease binding: crystal structures of the turkey ovomucoid third domain backbone variants OMTKY3-Pro18I and OMTKY3-psi[COO]-Leu18I in complex with Streptomyces griseus proteinase B (SGPB) and the structure of the free inhibitor, OMTKY-3-psi[CH2NH2+]-Asp19I
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305
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2001
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brenda
Joe, K.; Borgford, T.J.; Bennet, A.J.
Generation of a thermostable and denaturant-resistant peptide ligase
Biochemistry
43
7672-7677
2004
Streptomyces griseus
brenda
Jaswal, S.S.; Truhlar, S.M.; Dill, K.A.; Agard, D.A.
Comprehensive analysis of protein folding activation thermodynamics reveals a universal behavior violated by kinetically stable proteases
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347
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2005
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brenda
Truhlar, S.M.E.; Cunningham, E.L.; Agard, D.A.
The folding landscape of Streptomyces griseus protease B reveals the energetic costs and benefits associated with evolving kinetic stability
Protein Sci.
13
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2004
Streptomyces griseus
brenda
Lee, T.W.; Qasim, M.A.; Laskowski, M.; James, M.N.
Structural insights into the non-additivity effects in the sequence-to-reactivity algorithm for serine peptidases and their inhibitors
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2007
Streptomyces griseus
brenda
Qasim, M.A.; Song, J.; Markley, J.L.; Laskowski, M.
Cleavage of peptide bonds bearing ionizable amino acids at P(1) by serine proteases with hydrophobic S(1) pocket
Biochem. Biophys. Res. Commun.
400
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2010
Streptomyces griseus
brenda
Hemmi, H.; Kumazaki, T.; Kojima, S.; Yoshida, T.; Ohkubo, T.; Yokosawa, H.; Miura, K.; Kobayashi, Y.
Increasing the hydrolysis constant of the reactive site upon introduction of an engineered Cys14-Cys39 bond into the ovomucoid third domain from silver pheasant
J. Pept. Sci.
17
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2011
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Qasim, M.A.; Wang, L.; Qasim, S.; Lu, S.; Lu, W.; Wynn, R.; Yi, Z.P.; Laskowski, M.
Additivity-based design of the strongest possible turkey ovomucoid third domain inhibitors for porcine pancreatic elastase (PPE) and Streptomyces griseus protease B (SGPB)
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brenda