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3.4.21.57
mast
histamine
allergic
degranulation
chymase
anaphylaxis
basophil
mastocytosis
eosinophil
asthma
nafamostat
tryptase-induced
anti-tryptase
beta-tryptase
leech-derived
tryptase-positive
anaphylactic
ige-dependent
proteinase-activated
trypsin-like
chymase-positive
mast-cell
receptor-2
mesilate
anti-ige
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
Leu-proteinase, Leucine endopeptidase, Leucine-specific serine proteinase, Plant Leu-proteinase, Proteinase, leucine-specific, Proteinase, spinach serine (leucine specific), Spinach leucine-specific serine proteinase,
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Leucine-specific serine proteinase
-
-
-
-
Plant Leu-proteinase
-
-
-
-
Proteinase, leucine-specific
-
-
-
-
Proteinase, spinach serine (leucine specific)
-
-
-
-
Spinach leucine-specific serine proteinase
-
-
-
-
Leucine endopeptidase
-
-
-
-
Leucine endopeptidase
-
-
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Hydrolysis of proteins. Preferential cleavage: Leu-/- in small molecule substrates
preferential cleavage: Leu-+- in small molecule substrates, the term -+- depicts the point of cleavage
-
-
-
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hydrolysis of peptide bond
-
-
-
-
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azocasein + H2O
?
-
-
-
-
?
Azocoll + H2O
?
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-Gly 4-nitroanilide + H2O
Nalpha-benzoyl-Gly + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-Ala 4-nitroanilide + H2O
Nalpha-benzoyl-L-Ala + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-Arg 4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-Leu 4-nitroanilide + H2O
Nalpha-benzoyl-L-Leu + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-L-Lys 4-nitroanilide + H2O
Nalpha-benzoyl-L-Lys + 4-nitroaniline
-
-
-
-
?
Nalpha-benzoyl-Val 4-nitroanilide + H2O
Nalpha-benzoyl-Val + 4-nitroaniline
-
-
-
-
?
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
-
-
-
-
?
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Arg + 4-nitrophenol
-
-
-
-
?
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
-
-
-
-
?
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ile + 4-nitrophenol
-
-
-
-
?
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
-
-
-
-
?
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenol
-
-
-
-
?
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenol
-
-
-
-
?
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Ca2+
-
86% activation at 1 mM
Mg2+
-
56% activation at 1 mM
Mn2+
-
39% activation at 1 mM
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1,10-phenanthroline
-
20% inhibition at 0.01 mM, 55% inhibition at 0.1 mM, 64% inhibition at 0.5 mM
bestatin
-
6% inhibition at 0.001 mM, 39% inhibition at 0.005 mM, 54% inhibition at 0.01 mM, 57% inhibition at 0.1 mM
Cu2+
-
87% inhibition at 1 mM
diisopropyl fluorophosphate
-
-
EDTA
-
45% inhibition at 0.01 mM, 64% inhibition at 0.1 mM, 84% inhibition at 0.5 mM
EGTA
-
17% inhibition at 0.01 mM, 44% inhibition at 0.1 mM, 44% inhibition at 0.5 mM
Fe2+
-
100% inhibition at 0.1 mM
Nalpha-tosyl-L-Leu chloromethyl ketone
-
-
phenylmethylsulfonyl fluoride
Soybean Bowman-Birk proteinase inhibitor
-
and its chymotrypsin and trypsin inhibiting fragments
-
phenylmethylsulfonyl fluoride
-
67% inhibition at 0.01 mM, 100% inhibition above 0.1 mM
phenylmethylsulfonyl fluoride
-
-
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0.315
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
50 mM Tris-HCl pH 8.0, 37°C, 10 min
0.25
Nalpha-benzoyl-Ala 4-nitroanilide
-
-
0.89
Nalpha-benzoyl-Gly 4-nitroanilide
-
-
0.47
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
-
0.05
Nalpha-benzoyl-Leu 4-nitroanilide
-
-
0.45
Nalpha-benzoyl-Lys 4-nitroanilide
-
-
0.18
Nalpha-benzoyl-Val 4-nitroanilide
-
-
0.12
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
-
-
0.28
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
-
-
0.52
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
-
-
0.065
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
-
-
0.018
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
-
-
0.26
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
-
-
0.066
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
-
-
additional information
additional information
-
Km values are pH-independent
-
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1.2
Nalpha-benzoyl-Gly 4-nitroanilide
-
-
4.9
Nalpha-benzoyl-L-Ala 4-nitroanilide
-
-
2.1
Nalpha-benzoyl-L-Arg-4-nitroanilide
-
-
1.8
Nalpha-benzoyl-L-Lys 4-nitroanilide
-
-
21.3
Nalpha-benzoyl-Leu 4-nitroanilide
-
-
6.1
Nalpha-benzoyl-Val 4-nitroanilide
-
-
94
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
-
-
43
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
-
-
21
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
-
-
140
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
-
-
430
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
-
-
45
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
-
-
140
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
-
-
additional information
additional information
-
the acylation step is the rate-limiting in catalysis
-
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0.0067
bestatin
-
50 mM Tris-HCl pH 8.0, 37°C, 10 min
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additional information
-
pI: 4.8
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10 - 70
-
30% of activity at 10°C and 70°C
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-
-
-
brenda
-
-
-
brenda
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-
-
brenda
-
-
brenda
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60000
-
Spinacia oleracea, gel filtration
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70
-
complete loss of acivity after 30 min at 70°C
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Aducci, P.; Ascenzi, P.; Pierini, M.; Ballio, A.
Purification and characterization of Leu-proteinase, the leucine specific serine proteinase from spinach (Spinacia oleracea L.) leaves
Plant Physiol.
81
812-816
1986
Spinacia oleracea
brenda
Aducci, P.; Ascenzi, P.; Ballio, A.
Esterolytic properties of leucine-proteinase, the leucine-specific serine proteinase from spinach (Spinacia oleracea L.) leaves. A steady-state and pre-steady-state study
Plant Physiol.
82
591-593
1986
Spinacia oleracea
brenda
Ascenzi, P.; Amiconi, G.; Ballio, A.; Bolognesi, M.; Menegatti, E.; Schnebli, H.P.; Aducci, P.
Binding of the recombinant proteinase inhibitor eglin c, of the soybean Bowman-Birk proteinase inhibitor and of its chymotrypsin and trypsin inhibiting fragments to Leu-proteinase, the leucine specific serine proteinase from spinach (Spinacia oleracea L.) leaves: thermodynamic study
J. Enzyme Inhib.
4
283-288
1991
Spinacia oleracea
brenda
Kim, J.I.; Lee, S.M.; Jung, H.J.
Characterization of calcium-activated bifunctional peptidase of the psychrotrophic Bacillus cereus
J. Microbiol.
43
237-243
2005
Bacillus cereus
brenda
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3.4.21.57 Leucyl endopeptidase
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