Information on EC 3.4.21.57 - Leucyl endopeptidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

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EC NUMBER
COMMENTARY hide
3.4.21.57
-
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RECOMMENDED NAME
GeneOntology No.
Leucyl endopeptidase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins. Preferential cleavage: Leu-/- in small molecule substrates
show the reaction diagram
preferential cleavage: Leu-+- in small molecule substrates, the term -+- depicts the point of cleavage
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
136396-22-0
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
-
-
-
-
-
Azocoll + H2O
?
show the reaction diagram
-
-
-
-
-
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-Gly 4-nitroanilide + H2O
Nalpha-benzoyl-Gly + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Nalpha-benzoyl-L-Ala 4-nitroanilide + H2O
Nalpha-benzoyl-L-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Nalpha-benzoyl-L-Arg 4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Nalpha-benzoyl-L-Leu 4-nitroanilide + H2O
Nalpha-benzoyl-L-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Nalpha-benzoyl-L-Lys 4-nitroanilide + H2O
Nalpha-benzoyl-L-Lys + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Nalpha-benzoyl-Val 4-nitroanilide + H2O
Nalpha-benzoyl-Val + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Arg + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ile + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenol
show the reaction diagram
-
-
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
86% activation at 1 mM
Mg2+
-
56% activation at 1 mM
Mn2+
-
39% activation at 1 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
20% inhibition at 0.01 mM, 55% inhibition at 0.1 mM, 64% inhibition at 0.5 mM
bestatin
-
6% inhibition at 0.001 mM, 39% inhibition at 0.005 mM, 54% inhibition at 0.01 mM, 57% inhibition at 0.1 mM
Cu2+
-
87% inhibition at 1 mM
diisopropyl fluorophosphate
-
-
EDTA
-
45% inhibition at 0.01 mM, 64% inhibition at 0.1 mM, 84% inhibition at 0.5 mM
Eglin c
-
-
EGTA
-
17% inhibition at 0.01 mM, 44% inhibition at 0.1 mM, 44% inhibition at 0.5 mM
Fe2+
-
100% inhibition at 0.1 mM
Nalpha-tosyl-L-Leu chloromethyl ketone
-
-
phenylmethylsulfonyl fluoride
Soybean Bowman-Birk proteinase inhibitor
-
and its chymotrypsin and trypsin inhibiting fragments
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.63
azocasein
-
-
-
0.315
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
-
50 mM Tris-HCl pH 8.0, 37°C, 10 min
0.25
Nalpha-benzoyl-Ala 4-nitroanilide
-
-
0.47
Nalpha-benzoyl-Arg 4-nitroanilide
-
-
0.89
Nalpha-benzoyl-Gly 4-nitroanilide
-
-
0.05
Nalpha-benzoyl-Leu 4-nitroanilide
-
-
0.45
Nalpha-benzoyl-Lys 4-nitroanilide
-
-
0.18
Nalpha-benzoyl-Val 4-nitroanilide
-
-
0.12
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
-
-
0.28
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
-
-
0.52
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
-
-
0.065
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
-
-
0.018
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
-
-
0.26
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
-
-
0.066
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
-
-
additional information
additional information
-
Km values are pH-independent
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
Nalpha-benzoyl-Arg 4-nitroanilide
-
-
1.2
Nalpha-benzoyl-Gly 4-nitroanilide
-
-
4.9
Nalpha-benzoyl-L-Ala 4-nitroanilide
-
-
1.8
Nalpha-benzoyl-L-Lys 4-nitroanilide
-
-
21.3
Nalpha-benzoyl-Leu 4-nitroanilide
-
-
6.1
Nalpha-benzoyl-Val 4-nitroanilide
-
-
94
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
-
-
43
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
-
-
21
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
-
-
140
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
-
-
430
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
-
-
45
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
-
-
140
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
-
-
additional information
additional information
-
the acylation step is the rate-limiting in catalysis
-
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0067
bestatin
-
50 mM Tris-HCl pH 8.0, 37°C, 10 min
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
approximately
additional information
-
pI: 4.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
-
-
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 70
-
30% of activity at 10°C and 70°C
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
-
Spinacia oleracea, gel filtration
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
complete loss of acivity after 30 min at 70°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity
-
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LINKS TO OTHER DATABASES (specific for EC-Number 3.4.21.57)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)