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Information on EC 3.4.21.57 - Leucyl endopeptidase for references in articles please use BRENDA:EC3.4.21.57Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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Hydrolysis of proteins. Preferential cleavage: Leu-/- in small molecule substrates
preferential cleavage: Leu-+- in small molecule substrates, the term -+- depicts the point of cleavage
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hydrolysis of peptide bond
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-
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Leucine-specific serine proteinase
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Plant Leu-proteinase
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Proteinase, leucine-specific
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Proteinase, spinach serine (leucine specific)
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Spinach leucine-specific serine proteinase
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Leucine endopeptidase
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Leucine endopeptidase
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-
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brenda
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brenda
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azocasein + H2O
?
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-
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-
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Azocoll + H2O
?
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-
-
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-
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
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-
-
-
?
Nalpha-benzoyl-Gly 4-nitroanilide + H2O
Nalpha-benzoyl-Gly + 4-nitroaniline
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-
-
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Nalpha-benzoyl-L-Ala 4-nitroanilide + H2O
Nalpha-benzoyl-L-Ala + 4-nitroaniline
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-
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Nalpha-benzoyl-L-Arg 4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
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-
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Nalpha-benzoyl-L-Leu 4-nitroanilide + H2O
Nalpha-benzoyl-L-Leu + 4-nitroaniline
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-
-
-
-
Nalpha-benzoyl-L-Lys 4-nitroanilide + H2O
Nalpha-benzoyl-L-Lys + 4-nitroaniline
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-
-
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-
Nalpha-benzoyl-Val 4-nitroanilide + H2O
Nalpha-benzoyl-Val + 4-nitroaniline
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-
-
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-
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ala + 4-nitrophenol
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-
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-
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Arg + 4-nitrophenol
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Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Gly + 4-nitrophenol
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Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Ile + 4-nitrophenol
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Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Leu + 4-nitrophenol
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Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Lys + 4-nitrophenol
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Nalpha-carbobenzoxy-Val 4-nitrophenyl ester + H2O
Nalpha-carbobenzoxy-Val + 4-nitrophenol
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Ca2+
-
86% activation at 1 mM
Mg2+
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56% activation at 1 mM
Mn2+
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39% activation at 1 mM
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1,10-phenanthroline
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20% inhibition at 0.01 mM, 55% inhibition at 0.1 mM, 64% inhibition at 0.5 mM
bestatin
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6% inhibition at 0.001 mM, 39% inhibition at 0.005 mM, 54% inhibition at 0.01 mM, 57% inhibition at 0.1 mM
Cu2+
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87% inhibition at 1 mM
diisopropyl fluorophosphate
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-
EDTA
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45% inhibition at 0.01 mM, 64% inhibition at 0.1 mM, 84% inhibition at 0.5 mM
EGTA
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17% inhibition at 0.01 mM, 44% inhibition at 0.1 mM, 44% inhibition at 0.5 mM
Fe2+
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100% inhibition at 0.1 mM
Nalpha-tosyl-L-Leu chloromethyl ketone
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phenylmethylsulfonyl fluoride
Soybean Bowman-Birk proteinase inhibitor
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and its chymotrypsin and trypsin inhibiting fragments
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phenylmethylsulfonyl fluoride
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67% inhibition at 0.01 mM, 100% inhibition above 0.1 mM
phenylmethylsulfonyl fluoride
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-
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0.315
N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide
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50 mM Tris-HCl pH 8.0, 37°C, 10 min
0.25
Nalpha-benzoyl-Ala 4-nitroanilide
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-
0.47
Nalpha-benzoyl-Arg 4-nitroanilide
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-
0.89
Nalpha-benzoyl-Gly 4-nitroanilide
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-
0.05
Nalpha-benzoyl-Leu 4-nitroanilide
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-
0.45
Nalpha-benzoyl-Lys 4-nitroanilide
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-
0.18
Nalpha-benzoyl-Val 4-nitroanilide
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-
0.12
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
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-
0.28
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
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-
0.52
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
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-
0.065
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
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-
0.018
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
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-
0.26
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
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-
0.066
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
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-
additional information
additional information
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Km values are pH-independent
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2.1
Nalpha-benzoyl-Arg 4-nitroanilide
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-
1.2
Nalpha-benzoyl-Gly 4-nitroanilide
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4.9
Nalpha-benzoyl-L-Ala 4-nitroanilide
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1.8
Nalpha-benzoyl-L-Lys 4-nitroanilide
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21.3
Nalpha-benzoyl-Leu 4-nitroanilide
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6.1
Nalpha-benzoyl-Val 4-nitroanilide
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94
Nalpha-carbobenzoxy-Ala 4-nitrophenyl ester
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43
Nalpha-carbobenzoxy-Arg 4-nitrophenyl ester
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21
Nalpha-carbobenzoxy-Gly 4-nitrophenyl ester
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140
Nalpha-carbobenzoxy-Ile 4-nitrophenyl ester
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430
Nalpha-carbobenzoxy-Leu 4-nitrophenyl ester
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45
Nalpha-carbobenzoxy-Lys 4-nitrophenyl ester
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140
Nalpha-carbobenzoxy-Val 4-nitrophenyl ester
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additional information
additional information
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the acylation step is the rate-limiting in catalysis
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0.0067
bestatin
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50 mM Tris-HCl pH 8.0, 37°C, 10 min
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additional information
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pI: 4.8
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10 - 70
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30% of activity at 10°C and 70°C
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brenda
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brenda
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60000
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Spinacia oleracea, gel filtration
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70
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complete loss of acivity after 30 min at 70°C
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Aducci, P.; Ascenzi, P.; Pierini, M.; Ballio, A.
Purification and characterization of Leu-proteinase, the leucine specific serine proteinase from spinach (Spinacia oleracea L.) leaves
Plant Physiol.
81
812-816
1986
Spinacia oleracea
brenda
Aducci, P.; Ascenzi, P.; Ballio, A.
Esterolytic properties of leucine-proteinase, the leucine-specific serine proteinase from spinach (Spinacia oleracea L.) leaves. A steady-state and pre-steady-state study
Plant Physiol.
82
591-593
1986
Spinacia oleracea
brenda
Ascenzi, P.; Amiconi, G.; Ballio, A.; Bolognesi, M.; Menegatti, E.; Schnebli, H.P.; Aducci, P.
Binding of the recombinant proteinase inhibitor eglin c, of the soybean Bowman-Birk proteinase inhibitor and of its chymotrypsin and trypsin inhibiting fragments to Leu-proteinase, the leucine specific serine proteinase from spinach (Spinacia oleracea L.) leaves: thermodynamic study
J. Enzyme Inhib.
4
283-288
1991
Spinacia oleracea
brenda
Kim, J.I.; Lee, S.M.; Jung, H.J.
Characterization of calcium-activated bifunctional peptidase of the psychrotrophic Bacillus cereus
J. Microbiol.
43
237-243
2005
Bacillus cereus
brenda
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