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Information on EC 3.4.21.46 - complement factor D

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.46 complement factor D
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UNIPROT: O43915 not found.
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
selective cleavage of Arg-/-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor
Synonyms
vascular endothelial growth factor, vegf-d, adipsin, factor d, pdgf-d, complement factor d, vascular endothelial growth factor d, platelet-derived growth factor d, endogenous vascular elastase, podak, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
vascular endothelial growth factor
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vascular endothelial growth factor D
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28 kDa protein, adipocyte
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Adipsin
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-
-
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C3 convertase activator
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-
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C3 proactivator convertase
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-
-
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convertase, C3 proactivator
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-
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endogenous vascular elastase
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esterase, properdin factor D
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factor D
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factor D (complement)
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CAS REGISTRY NUMBER
COMMENTARY hide
37213-56-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
is mainly expressed in the cytoplasm of tumor cells
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
VEGFD_HUMAN
354
0
40444
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
SDS-PAGE and immunoblotting. VEGF-DDeltaNDeltaC homodimer is antiparallel and has an elongated shape but is flat at the central part. It has three subunit interface cysteines C25, C44 and C53
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C25A
has increased activity compared to the native VEGF-DDELTANDELTAC. Efficiently binds to the soluble receptor VEGFR-2/IgGFc. The C25A mutant behaves mainly as a monomeric protein on SDS-PAGE under reducing conditions but as a dimeric protein under non-reducing conditions. It induces VEGFR-2 Tyr-1175 phosphorylation
C25A/P43S
has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells
C25I
is mainly in a presumably covalently bound dimeric form
C25L
has increased activity compared to the native VEGF-DDELTANDELTAC, the C25L mutant is the most active mutant and is mainly in a presumably covalently bound dimeric form, has highest affinity to bind soluble receptor VEGFR-2/IgGFc. It induces VEGFR-2 Tyr-1175 phosphorylation
C25V
is mainly in a presumably covalently bound dimeric form
C44A
does not bind to the soluble receptor VEGFR-2/IgGFc
G51C
can not induce cell survival, has increased dimer to monomer ratio
P43S
can not induce cell survival
R22I
can not induce cell survival
R22I/C25L
has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells, has increased dimer to monomer ratio
R22L
can not induce cell survival
R22L/C25L
has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells, has increased dimer to monomer ratio
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
VEGF-DDELTANDELTAC and the three cysteine mutants purified by metal affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
mutants transiently transfected into 293T cells. The native VEGF-DDELTANDELTAC and mutants expressed in a larger scale in insect cells using BVboostFG baculovirus expression system
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
VEGF-D plays an essential role in tumoral lymphangiogenesis and lymphatic spread, VEGF-D expression, and the intratumoral lymphatics may be clinically useful indicators for prognostic evaluation in patients with epithelial ovarian carcinoma
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, L.; Liu, B.; Li, X.; Yang, S.; Xiao, J.; Chen, M.; Zhang, Y.; Ma, J.
Vascular endothelial growth factor D and intratumoral lymphatics as independent prognostic factors in epithelial ovarian carcinoma
Anat. Rec. (Hoboken)
292
562-569
2009
Homo sapiens (O43915), Homo sapiens
Manually annotated by BRENDA team
Toivanen, P.I.; Nieminen, T.; Viitanen, L.; Alitalo, A.; Roschier, M.; Jauhiainen, S.; Markkanen, J.E.; Laitinen, O.H.; Airenne, T.T.; Salminen, T.A.; Johnson, M.S.; Airenne, K.J.; Ylae-Herttuala, S.
Novel vascular endothelial growth factor D (VEGF-D) variants with increased biological activity
J. Biol. Chem.
284
16037-1648
2009
Homo sapiens (O43915)
Manually annotated by BRENDA team