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Information on EC 3.4.21.4 - trypsin

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.4 trypsin
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This record set is specific for:
UNIPROT: Q54137 not found.
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
preferential cleavage: Arg-/-, Lys-/-
Synonyms
trypsin, at-ii, cationic trypsinogen, beta-trypsin, trypsin-like enzyme, mesotrypsin, trypsin a, cationic trypsin, anionic trypsinogen, anionic trypsin, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-trypsin
-
-
-
-
Anionic trypsinogen
-
-
-
-
beta-trypsin
-
-
-
-
Brain trypsinogen
-
-
-
-
Cationic trypsinogen
-
-
-
-
cocoonase
-
-
-
-
Mesotrypsinogen
-
-
-
-
parenzyme
-
-
-
-
parenzymol
-
-
-
-
pseudotrypsin
-
-
-
-
SET
-
-
-
-
sperm receptor hydrolase
-
-
-
-
tripcellim
-
-
-
-
tryptar
-
-
-
-
tryptase
-
-
-
-
trypure
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9002-07-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
show the reaction diagram
Streptomyces erythraeus trypsin has more than 1 order of magnitude greater amidase activity than mammalian trypsin
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Soybean trypsin inhibitor
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
N-alpha-benzoyl-L-Arg-p-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
0.68
N-alpha-benzoyl-L-Lys-4-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.73
N-alpha-benzoyl-L-Arg-p-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
1.04
N-alpha-benzoyl-L-Lys-4-nitroanilide
recombinant enzyme, in 100 mM Tris-HCl, pH 8.0 at 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
trypsin-like protease; synonym Saccharopolyspora erythraea
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q54137_SACER
272
0
27793
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23630
trypsin inhibitor purified mature enzyme, calculated from amino acid sequence
28620
nickel purified His-tag-proenzyme, calculated from amino acid sequence
35000
trypsin inhibitor purified mature enzyme, SDS-PAGE
40000
nickel purified His-tag-proenzyme, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
the recombinant enzyme retains 44% of its maximum activity at pH 6.0
699820
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
in the so-called autolysis loop and shows no degradation after 96 h of incubation at 37°C
Streptomyces erythraeus trypsin is resistant to autolytic degradation due to the absence of an arginine or lysine residue
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
guanidine-HCl
the recombinant enzyme retains about 40 and 20% activity in 0.1 and 0.3 M guanidine-HCl
urea
the recombinant enzyme retains approximately 80 and 50% of its activity in 2 and 4 M urea, respectively
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+-TED-resin affinity column chromatography and soybean trypsin inhibitor affinity column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
recombinant Streptomyces erythraeus trypsin is fundamental to the use for proteomics applications
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kiser, J.Z.; Post, M.; Wang, B.; Miyagi, M.
Streptomyces erythraeus trypsin for proteomics applications
J. Proteome Res.
8
1810-1817
2009
Sus scrofa, Saccharopolyspora erythraea (Q54137), Saccharopolyspora erythraea
Manually annotated by BRENDA team