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(VGVAPG)2V + H2O
VGVAPGVGV + APGV + VGVAPG + VGVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
-
-
?
(VGVAPG)3V + H2O
VGVAPGVGV + APGVGVAPGV + VGVAPGVGVAPGVGV + APGV + VGVAPG + VGVAPGVGVAPGV + GVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
-
-
?
1-ethyl-3,3-dimethyl-2-[(E)-2-[6-(2,2,3,3,3-pentafluoropropanamido)-2,3-dihydro-1H-xanthen-4-yl]ethenyl]-3H-indol-1-ium + H2O
2-[(E)-2-(6-amino-2,3-dihydro-1H-xanthen-4-yl)ethenyl]-1-ethyl-3,3-dimethyl-3H-indol-1-ium + 2,2,3,3,3-pentafluoropropanal
-
-
-
-
ir
2-(chloroacetyl)-3-naphthoic acid o-toluidide + H2O
?
-
-
-
-
?
2-aminobenzoyl-APEEIMDQQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDQQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is 136times greater for neutrophil elastase than for proteinase 3
-
-
?
2-aminobenzoyl-APEEIMDRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDRQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is 47times greater for neutrophil elastase than for proteinase 3
-
-
?
2-aminobenzoyl-APEEIMDRYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDRYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is 30times greater for neutrophil elastase than for proteinase 3
-
-
?
2-aminobenzoyl-APEEIMDYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDYQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is 114times greater for neutrophil elastase than for proteinase 3
-
-
?
2-aminobenzoyl-APEEIMPRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MPRQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is over 11times greater for neutrophil elastase than for proteinase 3
-
-
?
2-aminobenzoyl-APEEIMRRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MRRQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is over 500times greater for neutrophil elastase than for proteinase 3
-
-
?
4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid + H2O
?
-
-
-
?
Abz-APEEIMDDQ-ethylene diamine 2,4 dinitrophenyl + H2O
2-aminobenzoyl-APEEI + MDDQ-N-(2,4-dinitrophenyl)ethylenediamine
-
kcat/Km is 45times greater for neutrophil elastase than for proteinase 3
-
-
?
Abz-QPMAVVQSVPQ-EDDnp + H2O
?
-
-
-
-
?
acetyl-Ala-Ala-Ala-p-nitroanilide + H2O
?
acetyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
?
-
-
-
-
?
acetyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
acetyl-Ala-Ala-Pro-Val + 4-nitroaniline
-
-
-
-
?
acetyl-L-Phe-2-naphthyl ester + H2O
acetyl-L-Phe + 2-naphthol
-
-
-
-
?
alpha1-PI + H2O
?
-
important antiprotease of the lung, enzyme reduces the lungs primary protection against proteolysis
-
-
?
Antithrombin III + H2O
?
degradation
-
-
?
APG(VGVAPG)2 + H2O
APGVGV + APGVAPGV + APGVGVAPGVGV + APGVGVAPG + APGV + VGVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
-
-
?
azocasein + H2O
?
-
-
-
-
?
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole + H2O
?
-
-
-
-
?
benzyloxycarbonyl-Ala-4-nitrophenol + H2O
benzyloxycarbonyl-Ala + 4-nitrophenol
-
-
-
-
?
benzyloxycarbonyl-L-Ala 2-naphthyl ester + H2O
benzyloxycarbonyl-Ala + 2-naphthol
-
-
-
-
?
Bovine serum albumin + H2O
?
-
-
-
-
?
BP180/type XVII collagen + H2O
peptide p561 + peptide p506
-
neutrophil elastase degrades recombinant mouse BP180 within the immunodominant extracellular domain at amino acid positions 506 and 561
-
-
?
Cartilage proteoglycan + H2O
?
-
-
-
-
?
CD2 + H2O
?
-
CD2 on peripheral blood T lymphocytes
-
-
?
CD4 + H2O
?
-
CD4 on peripheral blood T lymphocytes
-
-
?
CD8 + H2O
?
-
CD8 on peripheral blood T lymphocytes
-
-
?
coagulation factor V + H2O
?
-
-
-
-
?
coagulation factor VIII + H2O
?
-
-
-
-
?
coagulation factor XI + H2O
?
-
-
-
-
?
coagulation factor XIII + H2O
?
-
-
-
-
?
Collagen type I + H2O
?
-
-
-
-
?
collagen type II + H2O
?
-
-
-
-
?
Collagen type III + H2O
?
-
-
-
-
?
collagen type IV + H2O
?
-
-
-
-
?
collagenase + H2O
?
-
-
-
-
?
complement factor C3 + H2O
?
-
-
-
-
?
complement factor C5 + H2O
?
-
-
-
-
?
complement receptor + H2O
?
-
-
-
-
?
corticosteroid-binding globulin + H2O
?
Cut homeobox 1 + H2O
?
-
-
-
-
?
EMILIN1 + H2O
?
-
-
-
-
?
Factor VIIIa + H2O
?
-
neutrophil elastase inactivates factor VIIIa. The enzyme proteolyses the heavy chain of factor VIIIa into two terminal products, A11-358 and A2375-708, by limited proteolysis at Val358, Val374, and Val708
-
-
?
Fibronectin + H2O
?
-
-
-
-
?
Gelatinase + H2O
?
-
-
-
-
?
gp120 protein + H2O
?
-
-
-
-
?
GRGGAGGRG + H2O
GRGGA + GGRG
-
-
-
-
?
GRGGFGGRG + H2O
GRGGF + GGRG
-
-
-
-
?
GRGGLGGRG + H2O
GRGGL + GGRG
-
-
-
-
?
GRGGRGGRG + H2O
GRGGR + GGRG
-
-
-
-
?
GRGGVGGRG + H2O
GRGGV + GGRG
-
-
-
-
?
heparin-antithrombin complex + H2O
?
-
-
-
-
?
histone H1 + H2O
?
-
-
-
-
?
histone H2A ending on Val114 + H2O
VTIAQGGVLPNIQAV + ?
-
-
-
-
?
histone H4 + H2O
?
-
-
-
-
?
human IgG3 + H2O
?
-
-
-
-
?
human SP-D protein + H2O
SP-D protein peptide fragments
IgA + H2O
?
-
involved in the regulation of inflammation by a feedback mechanism, elastase treated IgA preparations are unable to induce oxidative burst of polymorphonuclear leukocytes
protein cleaved in the hinge region
-
?
IgG + H2O
?
-
involved in the regulation of inflammation by a feedback mechanism, elastase treated IgG preparations are unable to induce oxidative burst of polymorphonuclear leukocytes
protein cleaved in the hinge region
-
?
immunoglobulin A + H2O
?
-
-
-
-
?
immunoglobulin G + H2O
?
-
-
-
-
?
immunoglobulin M + H2O
?
-
-
-
-
?
insulin receptor substrate-1 + H2O
?
-
-
-
-
?
intercellular adesion molecule-1 + H2O
?
-
-
-
-
?
intercellular adhesion molecule-1 + H2O
?
-
-
-
-
?
interleukin-1 + H2O
?
-
-
-
-
?
interleukin-2 + H2O
?
-
-
-
-
?
interleukin-36 receptor antagonist + H2O
activated interleukin-36 receptor antagonist V2 + ?
-
the enzyme cleaves 9 amino acids upstream of the A-X-D consensus of interleukin-36 receptor antagonist
-
-
?
interleukin-6 + H2O
?
-
-
-
-
?
IpaB protein + H2O
?
-
neutrophil elastase cleaves IpaB protein after alanine, valine, isoleucine, or threonine residues
-
-
?
kininogen + H2O
?
-
-
-
-
?
Laminin + H2O
?
-
-
-
-
?
laminin-332 + H2O
?
-
neutrophil elastase shows the greatest activity toward the gamma2 chain of laminin-332, in addition, neutrophil elastase cleaves a synthetic peptide derived from the region of human laminin gamma2 containing the matrix metalloproteinase-2 cleavage site, suggesting that neutrophil elastase may generate laminin-332 fragments that are also promigratory
-
-
?
lung surfactant + H2O
?
-
-
-
-
?
MAAPV-p-nitroanilide + H2O
MAAPV + p-nitroaniline
-
-
-
-
?
major outer membrane protein F + H2O
?
-
-
-
-
?
matrix metalloprotease 9 + H2O
matrix metalloprotease 9 peptide fragments
-
i.e. MMP-9, neutrophil elastase cleaves and activates at two specific cleavage sites, between Val38 and Ala39, and between Ala 39 and Glu40
-
-
?
matrix metalloproteinase-7 + H2O
?
-
human leukocyte elastase may be a natural inactivator of matrix metalloproteinase-7 which can counteract matrix metalloproteinase-7-induced apoptosis resistance
-
-
?
methoxysuccinyl-Ala-Ala-Pro-Ala-4-nitroanilide
?
-
-
-
-
?
methoxysuccinyl-Ala-Ala-Pro-Ala-thiobenzyl ester
?
-
-
-
-
?
methoxysuccinyl-Ala-Ala-Pro-alpha-aminobutyryl-4-nitroanilide + H2O
?
-
-
-
-
?
methoxysuccinyl-Ala-Ala-Pro-Met-4-nitroanilide + H2O
?
-
-
-
-
?
methoxysuccinyl-Ala-Ala-Pro-Val-4-methylcoumarin 7-amide + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 7-amino-4-methylcoumarin
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
?
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroanilide
-
-
-
-
?
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 7-amino-4-methylcoumarin
-
-
-
-
?
methoxysuccinyl-Ala-Ala-Pro-Val-thiobenzyl ester
?
-
-
-
-
?
methoxysuccinyl-Ala-Ile-Pro-Met-4-nitroanilide + H2O
?
-
-
-
-
?
methoxysuccinyl-Ala-Pro-Ala-4-nitroanilide
?
-
-
-
-
?
methoxysuccinyl-Ala-Pro-Ala-thiobenzyl ester
?
-
-
-
-
?
methoxysuccinyl-Ala-Pro-Val-4-nitroanilide
?
-
-
-
-
?
methoxysuccinyl-Ala-Pro-Val-thiobenzyl ester
?
-
-
-
-
?
methoxysuccinyl-Pro-Val-4-nitroanilide
?
-
-
-
-
?
methoxysuccinyl-Pro-Val-thiobenzyl ester
?
-
-
-
-
?
methoxysuccinyl-Val-4-nitroanilide
methoxysuccinyl-Val + 4-nitroaniline
-
-
-
-
?
methoxysuccinyl-Val-thiobenzyl ester
?
-
-
-
-
?
N-(4-(4-morpholinylcarbonyl)benzoyl)-Val-Pro-Ile + H2O
?
-
-
-
?
N-methoxy-succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
N-methoxy-succinyl-Ala-Ala-Pro-Val + 4-nitroaniline
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
N-methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin + H2O
N-methoxysuccinyl-Ala-Ala-Pro-Val + 7-amino-4-methylcoumarin
-
-
-
-
?
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide + H2O
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val + 4-nitroaniline
-
-
-
-
?
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val-7-amido-4-methylcoumarin + H2O
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val + 7-amino-4-methylcoumarin
-
-
-
-
?
N-succinyl-Ala-Ala-Ala 4-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + 4-nitroaniline
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
-
-
-
?
N-succinyl-Ala-Ala-Val 4-nitroanilide + H2O
N-succinyl-Ala-Ala-Val + 4-nitroaniline
Nalpha-methoxysuccinyl-Nepsilon-2-picolinoyl-L-lysinyl-L-Ala-L-Pro-L-Val 4-nitroanilide + H2O
?
-
-
-
-
?
native type I collagen + H2O
3/4 length fragments similar in size to those produced by interstitial collagenase
-
human, bovine and rat type I collagen, the enzyme can cleave native type I collagen in the helix
-
?
o-aminobenzoic acid-Ala-Pro-Glu-Glu-Ile-Met-Asp-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Glu-Ala-Ile-Pro-Met-Ser-Ile-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Glu-Ala-Ile-Pro-Met-Ser-Ile-Pro-Pro-Glu-Val-Lys-Phe-Asn-Lys-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Gly-Ile-Ala-Thr-Phe-Cys-Met-Leu-Met-Pro-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Ile-Val-Ser-Ala-Arg-Met-Ala-Pro-Glu-Glu-Ile-Ile-Met-Asp-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Met-Met-Arg-Cys-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Thr-Phe-Cys-Met-Leu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Val-Ala-Asp-Cys-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Val-Ala-Glu-Cys-Cys-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
o-aminobenzoic acid-Val-Ser-Ala-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
oxidized B chain of insulin + H2O
?
oxytalan fibre + H2O
?
-
the elastase degrades the interfibrillar substances in the periodontal ligament and exposed individual collagen fibrils but not the collagen fibers of the transverse sections of rat mandibular first molars
-
-
?
plasminogen + H2O
?
-
-
-
-
?
plasminogen + H2O
miniplasminogen + ?
-
-
-
-
?
plasminogen activator inhibitor type-1 + H2O
?
-
-
-
-
?
platelet IIb/IIIa receptor + H2O
?
-
-
-
-
?
procarboxypeptidase R + H2O
carboxypeptidase R + ?
-
generation of activated carboxypeptidase R from procarboxypeptidase R by elastase from activated neutrophils at the site of inflammation should contribute to the suppression of excessive inflammation and any abnormality in this mechanism can induce serious hyper-inflammation such as seen in multiple organ failure
-
-
?
progranulin + H2O
?
-
-
-
-
?
progranulin + H2O
granulin + ?
-
-
-
-
?
Protein + H2O
?
-
enzyme capable of degrading proteins of the lung extracellular matrix in vitro
-
-
?
protein C + H2O
small peptides
proteinase-activated receptor 2 N-terminus + H2O
GPNSKGRSLIGRLDTPPPITGKGAPVEPGFSVDEFSAS + VLTGKLTTVFLP
-
-
-
-
?
secretory leucoprotease inhibitor + H2O
?
secretory leukocyte protease inhibitor + H2O
?
-
-
-
?
SLPI + H2O
?
-
important antiprotease of the lung, enzyme reduces the lungs primary protection against proteolysis
-
-
?
SP-D protein + H2O
SP-D protein peptide fragments
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
succinyl-Ala-Ala-Ala + p-nitroaniline
-
-
-
-
?
succinyl-Ala-Ala-NH-4-nitroanilide + H2O
succinyl-Ala-Ala-NH2 + 4-nitroaniline
succinyl-Ala-Ala-Pro-Ala-4-nitroanilide + H2O
?
-
-
-
-
?
succinyl-Ala-Ala-Pro-Ile-4-nitroanilide + H2O
?
-
-
-
-
?
succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
?
-
-
-
-
?
succinyl-Tyr-Leu-Val-4-nitroanilide + H2O
?
-
-
-
-
?
syndecan-1 + H2O
?
-
the heparan sulfate moiety of shed syndecan-1 binds and restricts neutrophil elastase from inhibition by alpha-1-antitrypsin
-
-
?
tert-butyloxycarbonyl-Ala-2-naphthyl ester + H2O
tert-butyloxycarbonyl-Ala + 2-naphthol
-
-
-
-
?
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester + H2O
tert-butyloxycarbonyl-Ala + 4-nitrophenol
tert-butyloxycarbonyl-L-Val-4-nitrophenyl ester + H2O
tert-butyloxycarbonyl-L-Val + 4-nitrophenol
-
-
-
-
?
tert-butyloxycarbonyl-Val-4-nitrophenyl ester + H2O
tert-butyloxycarbonyl-Val + 4-nitrophenol
-
-
-
-
?
thrombomodulin + H2O
?
-
-
-
-
?
tissue inhibitor of metalloprotease + H2O
?
-
-
-
-
?
tissue inhibitor of metalloprotease-1 + H2O
tissue inhibitor of metalloprotease-1 peptide fragments
-
i.e. TIMP-1, neutrophil elastase degrades TIMP-1 and cleaves between Val69-Cys70
-
-
?
tissue necrosis factor alpha + H2O
?
-
-
-
-
?
tosyl-Ala-3-hydroxy-5-phenylpyrrole + H2O
?
-
-
-
-
?
tosyl-Ala-4-nitrophenol + H2O
tosyl-Ala + 4-nitrophenol
-
-
-
-
?
tropoelastin + H2O
?
-
-
-
-
?
VAPGVGVAPGV + H2O
VAPGVGV + APGV + VAPGV + GVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
-
-
?
VLLFSEVL + H2O
VLLF + SEVL
-
-
-
-
?
VLLISEVL + H2O
VLLI + SEVL
-
-
-
-
?
VLLVSEVL + H2O
VLLV + SEVL
-
-
-
-
?
H2 heavy chain of inter-alpha-trypsin inhibitor + H2O
additional information
-
acetyl-Ala-Ala-Ala-p-nitroanilide + H2O

?
-
-
-
-
?
acetyl-Ala-Ala-Ala-p-nitroanilide + H2O
?
-
weak
-
-
?
annexin 1 + H2O

?
-
annexin 1 is externalized and truncated upon adhesion of polymorphonuclear leukocytes to endothelial cells, annexin 1 participates in regulating leukocyte emigration into inflamed tissue through N-terminal peptides generated at inflammatory sites, overview
-
-
?
annexin 1 + H2O
?
-
cleavage of N-terminal peptides, recombinant substrate
-
-
?
antithrombin + H2O

?
-
catalyzes the inactivation of antithrombin by a specific and limited proteinolytic cleavage
-
-
?
antithrombin + H2O
?
-
leukocyte elastase inactivates antithrombin by a specific and limited proteolytic cleavage
-
-
?
Collagen + H2O

?
-
-
-
-
?
Collagen + H2O
?
-
type I, II, III, and IV
-
-
?
Collagen + H2O
?
degradation
-
-
?
corticosteroid-binding globulin + H2O

?
-
cleavage by neutrophil elastase destroys ligand binding capacity and supposedly liberates cortisol at sites of inflammation. Enzyme activity on wild-type and mutant CBG serpin recombinantly produced by 293-EBNA cells, overview. CBG mutant G335V