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Information on EC 3.4.21.37 - leukocyte elastase
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3.4.21.37 leukocyte elastaseSpecify your search results
Word Map
- 3.4.21.37
- pulmonary
- cathepsin
- airway
- pancreatic
-
lavage
- myeloperoxidase
- hne
- emphysema
- fibrosis
-
cystic
-
bronchoalveolar
- granule
- elastin
- sputum
- bronchial
- thrombin
- chymotrypsin
- coagulation
-
1-antitrypsin
- neutropenia
- interleukin-8
-
1-proteinase
-
degranulation
-
c-reactive
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cardiopulmonary
-
elastolytic
-
antiprotease
- serpins
- mucus
- slpi
- alpha1-antitrypsin
-
azurophilic
- aa
-
neutrophil-derived
- elastases
-
2-macroglobulin
- bronchiectasis
- fmlp
-
elastase-induced
-
netosis
-
antineutrophil
- proteinase-3
-
thrombin-antithrombin
- ulinastatin
-
crevicular
-
neutrophil-mediated
-
ne-induced
-
beta-thromboglobulin
- drug development
-
wegener
-
2-antiplasmin
- diagnostics
- medicine
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Hydrolysis of proteins, including elastin. Preferential cleavage Val-/- > Ala-/-
Synonyms
neutrophil elastase, leukocyte elastase, granulocyte elastase, pmn elastase, elane, pmn-elastase, polymorphonuclear leukocyte elastase, polymorphonuclear neutrophil elastase, neutrophil-elastase, lysosomal elastase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
bone marrow serine protease
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EC 3.4.21.11
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formerly, part transferred
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EC 3.4.4.7
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formerly, part transferred
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ELA2
Elane
elastase
elaszym
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-
-
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granulocyte elastase
HLE
HNE
leukocyte elastase
lysosomal elastase
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NE
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-
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neutrophil elastase
PMN elastase
PMNE
serine elastase
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elastase
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granulocyte elastase
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HNE
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HNE
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leukocyte elastase
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neutrophil elastase
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neutrophil elastase
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683043, 683056, 683536, 683956, 695509, 696938, 697718, 697826, 698305, 698311, 698394, 698506, 698834, 699427, 699523, 699737, 699857, 700135, 700570, 700848, 700892, 701181, 701184, 701431, 707645, 707673, 707710, 707735, 707850, 707913, 708182, 708208, 708305, 708306, 708387, 708680, 708691, 708812, 708813, 709357, 709363, 709383, 709493, 709714, 709937, 709989, 710206, 717334, 717369, 717378, 717832, 717895, 718417, 718423, 731124, 731591, 731991, 732294, 732438, 732564, 732600, 732758, 732907, 752482, 753664, 754279, 754758, 755135, 755426
neutrophil elastase
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neutrophil elastase
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PMN elastase
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-
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PMNE
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins, including elastin. Preferential cleavage Val-/- > Ala-/-
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
peptides
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CAS REGISTRY NUMBER
COMMENTARY
9004-06-2
eastase
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(VGVAPG)2V + H2O
VGVAPGVGV + APGV + VGVAPG + VGVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
-
-
?
(VGVAPG)3V + H2O
VGVAPGVGV + APGVGVAPGV + VGVAPGVGVAPGVGV + APGV + VGVAPG + VGVAPGVGVAPGV + GVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
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-
?
1-ethyl-3,3-dimethyl-2-[(E)-2-[6-(2,2,3,3,3-pentafluoropropanamido)-2,3-dihydro-1H-xanthen-4-yl]ethenyl]-3H-indol-1-ium + H2O
2-[(E)-2-(6-amino-2,3-dihydro-1H-xanthen-4-yl)ethenyl]-1-ethyl-3,3-dimethyl-3H-indol-1-ium + 2,2,3,3,3-pentafluoropropanal
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ir
2-aminobenzoyl-APEEIMDQQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDQQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is 136times greater for neutrophil elastase than for proteinase 3
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-
?
2-aminobenzoyl-APEEIMDRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDRQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is 47times greater for neutrophil elastase than for proteinase 3
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-
?
2-aminobenzoyl-APEEIMDRYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDRYQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is 30times greater for neutrophil elastase than for proteinase 3
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-
?
2-aminobenzoyl-APEEIMDYQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MDYQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is 114times greater for neutrophil elastase than for proteinase 3
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-
?
2-aminobenzoyl-APEEIMPRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MPRQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is over 11times greater for neutrophil elastase than for proteinase 3
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-
?
2-aminobenzoyl-APEEIMRRQ-N-(2,4-dinitrophenyl)ethylenediamine + H2O
2-aminobenzoyl-APEEI + MRRQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is over 500times greater for neutrophil elastase than for proteinase 3
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-
?
Abz-APEEIMDDQ-ethylene diamine 2,4 dinitrophenyl + H2O
2-aminobenzoyl-APEEI + MDDQ-N-(2,4-dinitrophenyl)ethylenediamine
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kcat/Km is 45times greater for neutrophil elastase than for proteinase 3
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-
?
acetyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
acetyl-Ala-Ala-Pro-Val + 4-nitroaniline
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-
-
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?
alpha1-PI + H2O
?
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important antiprotease of the lung, enzyme reduces the lungs primary protection against proteolysis
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-
?
APG(VGVAPG)2 + H2O
APGVGV + APGVAPGV + APGVGVAPGVGV + APGVGVAPG + APGV + VGVAPGV
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human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
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-
?
benzyloxycarbonyl-Ala-4-nitrophenol + H2O
benzyloxycarbonyl-Ala + 4-nitrophenol
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-
-
-
?
benzyloxycarbonyl-L-Ala 2-naphthyl ester + H2O
benzyloxycarbonyl-Ala + 2-naphthol
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-
-
-
?
BP180/type XVII collagen + H2O
peptide p561 + peptide p506
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neutrophil elastase degrades recombinant mouse BP180 within the immunodominant extracellular domain at amino acid positions 506 and 561
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-
?
Factor VIIIa + H2O
?
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neutrophil elastase inactivates factor VIIIa. The enzyme proteolyses the heavy chain of factor VIIIa into two terminal products, A11-358 and A2375-708, by limited proteolysis at Val358, Val374, and Val708
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-
?
IgA + H2O
?
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involved in the regulation of inflammation by a feedback mechanism, elastase treated IgA preparations are unable to induce oxidative burst of polymorphonuclear leukocytes
protein cleaved in the hinge region
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?
IgG + H2O
?
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involved in the regulation of inflammation by a feedback mechanism, elastase treated IgG preparations are unable to induce oxidative burst of polymorphonuclear leukocytes
protein cleaved in the hinge region
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?
interleukin-36 receptor antagonist + H2O
activated interleukin-36 receptor antagonist V2 + ?
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the enzyme cleaves 9 amino acids upstream of the A-X-D consensus of interleukin-36 receptor antagonist
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?
IpaB protein + H2O
?
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neutrophil elastase cleaves IpaB protein after alanine, valine, isoleucine, or threonine residues
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?
laminin-332 + H2O
?
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neutrophil elastase shows the greatest activity toward the gamma2 chain of laminin-332, in addition, neutrophil elastase cleaves a synthetic peptide derived from the region of human laminin gamma2 containing the matrix metalloproteinase-2 cleavage site, suggesting that neutrophil elastase may generate laminin-332 fragments that are also promigratory
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-
?
matrix metalloprotease 9 + H2O
matrix metalloprotease 9 peptide fragments
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i.e. MMP-9, neutrophil elastase cleaves and activates at two specific cleavage sites, between Val38 and Ala39, and between Ala 39 and Glu40
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-
?
matrix metalloproteinase-7 + H2O
?
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human leukocyte elastase may be a natural inactivator of matrix metalloproteinase-7 which can counteract matrix metalloproteinase-7-induced apoptosis resistance
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?
methoxysuccinyl-Ala-Ala-Pro-Val-4-methylcoumarin 7-amide + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 7-amino-4-methylcoumarin
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroanilide
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?
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin + H2O
methoxysuccinyl-Ala-Ala-Pro-Val + 7-amino-4-methylcoumarin
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?
methoxysuccinyl-Val-4-nitroanilide
methoxysuccinyl-Val + 4-nitroaniline
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?
N-methoxy-succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
N-methoxy-succinyl-Ala-Ala-Pro-Val + 4-nitroaniline
N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O
N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline
N-methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin + H2O
N-methoxysuccinyl-Ala-Ala-Pro-Val + 7-amino-4-methylcoumarin
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-
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?
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide + H2O
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val + 4-nitroaniline
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-
-
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?
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val-7-amido-4-methylcoumarin + H2O
N-methoxysuccinyl-L-Ala-L-Ala-L-Pro-L-Val + 7-amino-4-methylcoumarin
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?
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Ala + 4-nitroaniline
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?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
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?
Nalpha-methoxysuccinyl-Nepsilon-2-picolinoyl-L-lysinyl-L-Ala-L-Pro-L-Val 4-nitroanilide + H2O
?
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-
-
-
?
native type I collagen + H2O
3/4 length fragments similar in size to those produced by interstitial collagenase
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human, bovine and rat type I collagen, the enzyme can cleave native type I collagen in the helix
-
?
o-aminobenzoic acid-Ala-Pro-Glu-Glu-Ile-Met-Asp-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Glu-Ala-Ile-Pro-Met-Ser-Ile-Pro-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Glu-Ala-Ile-Pro-Met-Ser-Ile-Pro-Pro-Glu-Val-Lys-Phe-Asn-Lys-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Gly-Ile-Ala-Thr-Phe-Cys-Met-Leu-Met-Pro-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Ile-Val-Ser-Ala-Arg-Met-Ala-Pro-Glu-Glu-Ile-Ile-Met-Asp-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Met-Met-Arg-Cys-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
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?
o-aminobenzoic acid-Thr-Phe-Cys-Met-Leu-Glu-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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?
o-aminobenzoic acid-Val-Ala-Asp-Cys-Ala-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Val-Ala-Glu-Cys-Cys-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
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-
-
-
?
o-aminobenzoic acid-Val-Ser-Ala-Arg-Gln-N-(2,4-dinitrophenyl)ethylenediamine + H2O
?
-
-
-
-
?
oxytalan fibre + H2O
?
-
the elastase degrades the interfibrillar substances in the periodontal ligament and exposed individual collagen fibrils but not the collagen fibers of the transverse sections of rat mandibular first molars
-
-
?
procarboxypeptidase R + H2O
carboxypeptidase R + ?
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generation of activated carboxypeptidase R from procarboxypeptidase R by elastase from activated neutrophils at the site of inflammation should contribute to the suppression of excessive inflammation and any abnormality in this mechanism can induce serious hyper-inflammation such as seen in multiple organ failure
-
-
?
Protein + H2O
?
-
enzyme capable of degrading proteins of the lung extracellular matrix in vitro
-
-
?
proteinase-activated receptor 2 N-terminus + H2O
GPNSKGRSLIGRLDTPPPITGKGAPVEPGFSVDEFSAS + VLTGKLTTVFLP
-
-
-
-
?
SLPI + H2O
?
-
important antiprotease of the lung, enzyme reduces the lungs primary protection against proteolysis
-
-
?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
succinyl-Ala-Ala-Ala + p-nitroaniline
-
-
-
-
?
syndecan-1 + H2O
?
-
the heparan sulfate moiety of shed syndecan-1 binds and restricts neutrophil elastase from inhibition by alpha-1-antitrypsin
-
-
?
tert-butyloxycarbonyl-Ala-2-naphthyl ester + H2O
tert-butyloxycarbonyl-Ala + 2-naphthol
-
-
-
-
?
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester + H2O
tert-butyloxycarbonyl-Ala + 4-nitrophenol
tert-butyloxycarbonyl-L-Val-4-nitrophenyl ester + H2O
tert-butyloxycarbonyl-L-Val + 4-nitrophenol
-
-
-
-
?
tert-butyloxycarbonyl-Val-4-nitrophenyl ester + H2O
tert-butyloxycarbonyl-Val + 4-nitrophenol
-
-
-
-
?
tissue inhibitor of metalloprotease-1 + H2O
tissue inhibitor of metalloprotease-1 peptide fragments
-
i.e. TIMP-1, neutrophil elastase degrades TIMP-1 and cleaves between Val69-Cys70
-
-
?
VAPGVGVAPGV + H2O
VAPGVGV + APGV + VAPGV + GVAPGV
-
human elastin exon 24-derived peptide substrate, hydrolysis by the enzyme occurs when its active site is fully occupied by the substrate, cleavage at Val-Ala and at Gly-Val bonds, the latter to a lesser extent
-
-
?
annexin 1 + H2O
?
-
annexin 1 is externalized and truncated upon adhesion of polymorphonuclear leukocytes to endothelial cells, annexin 1 participates in regulating leukocyte emigration into inflamed tissue through N-terminal peptides generated at inflammatory sites, overview
-
-
?
annexin 1 + H2O
?
-
cleavage of N-terminal peptides, recombinant substrate
-
-
?
antithrombin + H2O
?
-
catalyzes the inactivation of antithrombin by a specific and limited proteinolytic cleavage
-
-
?
antithrombin + H2O
?
-
leukocyte elastase inactivates antithrombin by a specific and limited proteolytic cleavage
-
-
?
corticosteroid-binding globulin + H2O
?
-
cleavage by neutrophil elastase destroys ligand binding capacity and supposedly liberates cortisol at sites of inflammation. Enzyme activity on wild-type and mutant CBG serpin recombinantly produced by 293-EBNA cells, overview. CBG mutant G335V