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Information on EC 3.4.21.36 - pancreatic elastase
for references in articles please use BRENDA:EC3.4.21.36
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EC Tree 3 Hydrolases
3.4 Acting on peptide bonds (peptidases)
3.4.21 Serine endopeptidases
3.4.21.36 pancreatic elastase
IUBMB Comments
Formed by activation of proelastase from mammalian pancreas by trypsin. In peptidase family S1 (trypsin family). Formerly included in EC 3.4.21.11
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
- 3.4.21.36
- porcine
-
exocrine
- emphysema
-
fecal
- elastin
- pancreas
- leukocyte
- pulmonary
-
elastase-induced
- elastases
-
instil
-
intratracheal
- amylase
- abdominal
- alveolar
-
stool
- aortic
- aaa
-
elastolytic
- aneurysm
-
lavage
-
bronchoalveolar
- proteinases
-
emphysematous
-
airspace
- hne
-
intercept
- alpha-chymotrypsin
- steatorrhea
-
1-antitrypsin
-
2-macroglobulin
-
1-proteinase
-
elastase-treated
- analysis
-
elastase-mediated
- elastatinal
-
maldigestion
- isoamylase
-
cholangiopancreatography
-
alpha-1-proteinase
-
procarboxypeptidase
-
antiprotease
-
antielastase
-
intra-aortic
- medicine
- ovomucoid
-
non-pancreatic
-
pancreatoduodenectomy
-
carlsberg
- desmosine
-
3.4.21.1
-
elastase-like
showSynonyms
pancreatic elastase, elastase 1, elastase-1, cela1, serine elastase, pancreatic elastase-1, cela3b, chymotrypsin-like elastase, pancreatic elastase i, prt-201, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
chymotrypsin-like elastase
EC 3.4.21.11
-
-
formerly, part transferred
-
EC 3.4.4.7
-
-
formerly, part transferred
-
elastase
elastase 1
elaszym
-
-
-
-
pancreatic elastase
pancreatic elastase I
-
-
-
-
pancreatopeptidase E
-
-
-
-
peptidase, pancreato-, E
-
-
-
-
PPE
PRT-201
serine elastase
-
-
-
-
elastase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-/-
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
endopeptidase
-
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CAS REGISTRY NUMBER
COMMENTARY
848900-32-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
succinyl-Ala-Ala-Phe + 4-nitroaniline
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Pro-4-nitroanilide + H2O
succinyl-Ala-Ala-Pro + 4-nitroaniline
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide + H2O
succinyl-L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-alpha-aminobutyric acid-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
t-butyloxycarbonyl-Ala-4-nitrophenol ester + H2O
t-butyloxycarbonyl-Ala + 4-nitrophenol
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
succinyl-Ala-Ala-Ala + 4-nitroaniline
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
succinyl-Ala-Ala-Ala + 4-nitroaniline
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
succinyl-Ala-Ala-Ala + p-nitroaniline
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Ala-p-nitroanilide + H2O
succinyl-Ala-Ala-Ala + p-nitroaniline
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Pro-Ala-p-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Pro-Ile-p-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide + H2O
?
-
Substrates: -
Products: -
Products: -
?
tert-butyloxycarbonyl-Ala-p-nitrophenylester + H2O
?
-
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
NaCl
-
elastase 1: slight inhibition above 150 mM, elastase 2: 25-250 mM, activation
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2R,2'R,2''R,2'''R,3S,3'S,3''S,3'''S,4S,4'S,4''R)-2,2',2'''-tris(3,4-dihydroxyphenyl)-2''-(4-hydroxyphenyl)-3,3',3'',3''',4,4',4'',4'''-octahydro-2H,2'H,2''H,2'''H-4,8':4',8'':4'',8'''-quaterchromene-3,3',3'',3''',5,5',5'',5''',7,7',7'',7'''-dodecol
-
-
(2R,2'R,2''R,3S,3'S,3''S,4S,4'R)-2,2',2''-tris(3,4-dihydroxyphenyl)-3,3',3'',4,4',4''-hexahydro-2H,2'H,2''H-4,8':4',8''-terchromene-3,3',3'',5,5',5'',7,7',7''-nonol
-
-
1-O-(3-O-sulfo-beta-D-galactopyranosyl)-ceramide
-
0.001 mg elastase in 0.01 ml of 50 mM HEPES buffer at pH 7.5 with 0.01 mg inhibitor in 0.01 ml DMSO at 37° for 30 min
4-([(S)-1-[((S)-2-[[(RS)-3,3,3-trifluoro-1-isopropyl-2-oxopropyl]aminocarbonyl]pyrrolidin-1-yl-)carbonyl]-2-methylpropyl]aminocarbonyl)benzoic acid
-
Cholesterol sulfate
-
0.001 mg elastase in 0.09 ml of 50 mM HEPES buffer at pH 7.5 with 0.01 mg inhibitor in 0.01 ml DMSO at 37° for 30 min
FR130180
i.e. 4-([(S)-1-[((S)-2-[[(RS)-3,3,3-trifluoro-1-isopropyl-2-oxopropyl]-aminocarbonyl]pyrrolidin-1yl)carbonyl]-2-methylpropyl]aminocarbonyl) benzoic acid
N-(trifluoroacetyl)-alpha-aspartyl-N-[4-(trifluoromethyl)phenyl]alaninamide
-
reversible inhibitor
N-(trifluoroacetyl)leucyl-N-[4-(trifluoromethyl)phenyl]alaninamide
-
reversible inhibitor
N-(trifluoroacetyl)valyl-N-[4-(trifluoromethyl)phenyl]alaninamide
-
reversible inhibitor
N-benzyl-2-oxo-4-(phenylsulfonyl)azetidine-1-carboxamide
-
JM54, potent and irreversible inhibitor
N2-(trifluoroacetyl)lysyl-N-[4-(trifluoromethyl)phenyl]alaninamide
-
reversible inhibitor
NaCl
-
elastase 1: slight inhibition above 150 mM, elastase 2: 25-250 mM, activation
scyptolin A
the inhibitor occupies the most prominent subsites S1 through S4 of the elastase and prevents a hydrolytic attack by covering the active center with its rigid ring structure
SDS
-
0.001 mg elastase in 0.09 ml of 50 mM HEPES buffer at pH 7.5 with 0.01 mg inhibitor in 0.01 ml DMSO at 37° for 30 min
serpin
active site distortion is sufficient for proteinase inhibition by serpins
-
viral serpin crmA
-
pancreatic elastase is rapidly inhibited by wild-type crmA and reactive loop variant P1 Arg crmAs
-
HEI-TOE I
-
hybrid inhibitor from Ecballium elaterium and the optimized binding loop of the third domain of turkey ovomucoid inhibitor
-
HEI-TOE I
hybrid inhibitor from Ecballium elaterium and the optimized binding loop of the third domain of turkey ovomucoid inhibitor
-
OMTKY3
third Kazal-type domain of ovomucoid inhibitor from turkey egg white
-
Schistocerca gregaria proteinase inhibitor 2 variant E1
-
Tyr-Cys-Thr-Leu-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E1
-
Tyr-Cys-Thr-Leu-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E10
-
Ala-Cys-Thr-Leu-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E10
-
Ala-Cys-Thr-Leu-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E11
-
Ala-Cys-Thr-Leu-Met-Tyr-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E11
-
Ala-Cys-Thr-Leu-Met-Tyr-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E12
-
Tyr-Cys-Thr-Leu-Met-Leu-Cys-Ala
-
Schistocerca gregaria proteinase inhibitor 2 variant E12
-
Tyr-Cys-Thr-Leu-Met-Leu-Cys-Ala
-
Schistocerca gregaria proteinase inhibitor 2 variant E2
-
Tyr-Cys-Thr-Ile-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E2
-
Tyr-Cys-Thr-Ile-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E3
-
Tyr-Cys-Thr-Val-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E3
-
Tyr-Cys-Thr-Val-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E4
-
Tyr-Cys-Thr-Met-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E4
-
Tyr-Cys-Thr-Met-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E5
-
Tyr-Cys-Thr-Ala-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E5
-
Tyr-Cys-Thr-Ala-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E6
-
Tyr-Cys-Thr-Ser-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E6
-
Tyr-Cys-Thr-Ser-Met-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E7
-
Tyr-Cys-Thr-Ile-Met-Glu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E7
-
Tyr-Cys-Thr-Ile-Met-Glu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E8
-
Tyr-Cys-Thr-Leu-Arg-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E8
-
Tyr-Cys-Thr-Leu-Arg-Leu-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E9
-
Tyr-Cys-Thr-Leu-Met-Tyr-Cys-His
-
Schistocerca gregaria proteinase inhibitor 2 variant E9
-
Tyr-Cys-Thr-Leu-Met-Tyr-Cys-His
-
additional information
-
serum from normal volunteers and patients with alpha 1-antitrypsin deficiency completely inactivate PRT-201 elastase activity in vitro
-
additional information
-
not inhibited by dehydroepiandrosterone sulfate, pregnenolone sulfate, cholesterol, oleic acid, triolein, phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, sphingomyelin, GalCer, LacCer
-
additional information
-
the peptide RC-12 does not inhibit pancreatic elastase at concentrations up to 0.1 mM
-
additional information
-
an oligomeric fraction of procyanidins shows about 90% inhibition at 0.2 mM
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
spermidine
-
spermidine serves as a partial stabilizer and an activator for the enzyme
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.555
succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
0.08229
4-nitrophenyl acetate
-
in the presence of 0.2 mM spermidine, at pH 8.5 and 37°C
0.09364
4-nitrophenyl acetate
-
in the presence of 0.3 mM spermidine, at pH 8.5 and 37°C
0.11568
4-nitrophenyl acetate
-
in the presence of 0.5 mM spermidine, at pH 8.5 and 37°C
2.09
succinyl-L-Ala-L-Ala-L-Ala-4-nitroanilide
-
in 0.1 M phosphate buffer, pH 7.0 and 37°C
0.166
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
0.374
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide
-
isoform CELA3B, at pH 8.0 and 22°C
1.089
succinyl-L-Ala-L-Ala-L-Pro-L-Ala-4-nitroanilide
-
isoform CELA3A, at pH 8.0 and 22°C
0.484
succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide
-
isoform CELA3B, at pH 8.0 and 22°C
0.995
succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
1.06
succinyl-L-Ala-L-Ala-L-Pro-L-Ile-4-nitroanilide
-
isoform CELA3A, at pH 8.0 and 22°C
0.259
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
isoform CELA3B, at pH 8.0 and 22°C
0.722
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
isoform CELA3A, at pH 8.0 and 22°C
0.785
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
0.288
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide
-
isoform CELA3B, at pH 8.0 and 22°C
0.452
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide
-
isoform CELA3A, at pH 8.0 and 22°C
0.54
succinyl-L-Ala-L-Ala-L-Pro-L-Met-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
0.984
succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide
-
isoform CELA3B, at pH 8.0 and 22°C
1.447
succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide
-
isoform CELA3A, at pH 8.0 and 22°C
2.311
succinyl-L-Ala-L-Ala-L-Pro-L-Ser-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
0.43
succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide
-
isoform CELA3B, at pH 8.0 and 22°C
1.029
succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide
-
isoform CELA1, at pH 8.0 and 22°C
1.178
succinyl-L-Ala-L-Ala-L-Pro-L-Val-4-nitroanilide
-
isoform CELA3A, at pH 8.0 and 22°C