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Information on EC 3.4.21.119 - kallikrein 13 and Organism(s) Homo sapiens and UniProt Accession Q9UKR3
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3.4.21.119 kallikrein 13Specify your search results
Word Map
- 3.4.21.119
- kallikreins
- prorenins
-
submandibular
- medicine
-
trypsin-like
-
endoproteolytic
- diagnostics
-
renin-angiotensin
- analysis
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
Synonyms
kallikrein 13, prece, mgk-13, prorenin converting enzyme, prorenin-converting enzyme, kallikrein-related peptidase 13, gk-13, glandular kallikrein 13, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
S01.306
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
enzyme is a serine protease
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
enzyme is a serine protease
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
342900-44-1
-
9001-01-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ABZ-Val-Arg-Phe-Arg-5-amino-2-nitrobenzoic acid + H2O
ABZ-Val-Arg-Phe-Arg + 5-amino-2-nitrobenzoic acid
kinetically preferred substrate
-
-
?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
Abz-MISLMKRPPGFSPFR + SSRI-NH2
-
rapid cleavage
-
-
?
FSR-amido-4-methylcoumarin + H2O
FSR + 7-amino-4-methylcoumarin
-
fluorogenic trypsin-like peptide substrate
-
-
?
histatin 3 + H2O
DSHAKRHHGYKRKFHEKHHSHRGYR + SNYLYDN
-
i.e. DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN, efficient substrate
-
-
?
myelin basic protein + H2O
?
-
at least two cleavage sites are identified at YGGR-ASDYK and PRHR-DTGI
-
-
?
VPR-amido-4-methylcoumarin + H2O
VPR + 7-amino-4-methylcoumarin
-
fluorogenic trypsin-like peptide substrate
-
-
?
additional information
?
-
-
enzyme is involved in tissue remodeling and/or tumor invasion and metastasis
-
-
?
additional information
?
-
-
KLK13 does not hydrolyze Abz-KLRESKQ-EDDnp, Abz-KLRSEKQ-EDDnp, and AbzeELRSSKQ-EDDnp
-
-
?
additional information
?
-
-
the S2' subsite of KLK13 does not have restricted specificity but does present preference for the basic amino acid R and does not accepted negative charge
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme is involved in tissue remodeling and/or tumor invasion and metastasis
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
Na+, K+, Mg2+ and Ca2+ up to 1 M concentration do not activate the enzyme
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0053
Abz-KLKSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.031
Abz-KLKSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.0012
Abz-KLRSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.0022
Abz-KLRSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.1
Abz-KLKSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.5
Abz-KLKSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
3.2
Abz-KLRSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
3.4
Abz-KLRSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
16
Abz-KLKSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
19
Abz-KLKSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
1455
Abz-KLRSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
2834
Abz-KLRSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
kallikrein-related peptidase 13 is downregulated in oral squamous cell carcinoma cell lines, primary tumors and established cell lines
-
expression of the KLK13 and KLK14 genes are examined at the mRNA and protein levels in a cohort of 57 patients with non-small-cell lung cancer. The mRNA levels, assessed by real-time RT-PCR, are significantly different in malignant tissues compared to adjacent non-malignant tissues. KLK13 and KLK14 mRNA overexpression in tumors is associated with a positive nodal status in multivariate analysis
-
the clinical value of 11 members of the tissue kallikrein family as potential biomarkers for lung cancer diagnosis are analyzed: Serum specimens from 51 patients with non-small cell lung cancer and from50 healthy volunteers are collected. Samples are analyzed for 11 kallikreins (KLK1, KLK4-8, and KLK10-14) by specific ELISA. compared with sera from normal subjects, sera of patients with non-small cell lung cancer have lower levels of KLK5, KLK7, KLK8, KLK10, and KLK12, and higher levels of KLK11, KLK13, and KLK14. KLK11, KLK12, KLK13, and KLK14 are associated with higher risk of non-small cell lung cancer as determined by univariate analysis and confirmed by multivariate analysis
additional information
-
quantification of eight different tissue kallikreins. Enzyme is present at significantly lower concentrations in females than in males
-
hK13 is present at significantly lower concentrations in females than in males
-
quantification of eight different tissue kallikreins. Enzyme is detected only in the eccrine sweat glands regardless of gender
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from the face with highest amounts, in the eccrine sweat glands regardless of gender
additional information
-
in cervicovaginal fluid at high concentration, in cervix at low concentration
additional information
-
with regard to abundance, hK13 is a minor hK. Minor hKs tend to show gender differences suggesting a sex hormone-dependent regulation
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
the enzyme belongs to the human kallikrein-related peptidase family, which is comprised of 15 serine protease genes on chromosome 19q13.4
physiological function
physiological function
-
KLK13 plays a role in the defense of the upper digestive apparatus and in male reproductive organs
physiological function
the enzyme may play an important role in regulating cellular migration and invasiveness
physiological function
serine proteases transmembrane protein serine 11D and kallikrein-related peptidase 13 act as active proteases in cervical-vaginal fluid. The proteolytic activity in cervical-vaginal fluid is highly pH-dependent with an almost absolute inhibition of trypsin-like proteolytic activity at physiological pH levels
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
enzyme forms SDS-stable complexes with serum protease inhibitors such as alpha2-antiplasmin, alpha2-macroglobulin and alpha1-antichymotrypsin
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
treatment of ovarian cancer cell lines secreting enzyme with an enzyme neutralizing antibody results in reduced migration of cells
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
KLK13 activity is reduced close to zero in the presence of 50 mM Tris HCl (with no sodium citrate or heparin)
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene KLK-L4, DNA sequence determination and analysis, gene mapps to chromosome 19q13.3-q13.4, tissue expression analysis
gene KLK13, encoded on chromosome 19q13.4, quantitative real-time PCR expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
kallikrein-related peptidase 13 is downregulated in oral squamous cell carcinoma cell lines
the kallikrein-related peptidase 13 gene is substantially up-regulated after exposure of gastric cancer cells to antineoplastic agents, epirubicin, oxaliplatin, or methotrexate
the KLK13 gene is dysregulated in several carcinomas, and its expression levels seem to be associated with disease prognosis
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
substrate substrate ABZ-Val-Arg-Phe-Arg-Ser-Thr-Gln-Tyr(3-NO2)-NH2 is suitable for determination of KLK13 activity and is successfully converted into an activity-based probe by the incorporation of a chloromethylketone warhead and biotin bait. The modified subvsrtae is applicable in complex biological samples
diagnostics
medicine
gene may be involved in the pathogenesis and/or progression of breast cancer and may find applicability as cancer biomarker
medicine
diagnostics
gene KLK13 mRNA expression constitutes a biomarker for the prediction of overall survival in nonsmall cell lung cancer and that its quantitative assessment in tumor tissues can aid in treatment decision making. Kaplan-Meier survival analyses demonstrate that patients with KLK13-positive tumors survive significantly longer than those with KLK13-negative ones
diagnostics
loss of the enzyme is a potential biomarker for early detection of lymph node metastasis in oral squamous cell carcinoma cell lines
diagnostics
the enzyme has clinical importance as a favorable prognostic biomarker for gastric cancer patients' survival
medicine
-
most salivary gland tumors show high levels of enzyme expression. Pleomorphic adenoma shows less staining than normal tissue, adenoid cystic carcinomas, polymorphous low grade adenocarcinomas and adenocarcinomas not otherwise specified stain significantly more than normal salivary gland tissue
medicine
-
patients with ovarian carcinoma, women with enzyme-positive tumors most often have early stage disease, no residual tumor after surgery and optimal debulking success. Patients with enzyme-positive tumors have a significantly longer progression-free survival and overall survival. Use of enzyme as biomarker for ovarian carcinoma
medicine
-
treatment of ovarian cancer cell lines secreting enzyme with an enzyme neutralizing antibody results in less migration of cells
medicine
-
a multiparametric panel of kallikrein markers for lung cancer diagnosis with relatively good accuracy is provided
medicine
-
although marginally significant, patients with high KLK13 expression at the mRNA or protein level have lower overall survival
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kapadia, C.; Chang, A.; Sotiropoulou, G.; Yousef, G.M.; Grass, L.; Soosaipillai, A.; Xing, X.; Howarth, D.H.; Diamandis, E.P.
Human kallikrein 13: production and purification of recombinant protein and monoclonal and polyclonal antibodies, and development of a sensitive and specific immunofluorometric assay
Clin. Chem.
49
77-86
2003
Homo sapiens
Yousef G.M.; Chang A.; Diamandis E.P.
Identification and characterization of KLK-L4, a new kallikrein-like gene that appears to be down-regulated in breast cancer tissues
J. Biol. Chem.
275
11891-11898
2000
Homo sapiens (Q9UKR3)
Kapadia, C.; Ghosh, M.C.; Grass, L.; Diamandis, E.P.
Human kallikrein 13 involvement in extracellular matrix degradation
Biochem. Biophys. Res. Commun.
323
1084-1090
2004
Homo sapiens
Kapadia, C.; Yousef, G.M.; Mellati, A.A.; Magklara, A.; Wasney, G.A.; Diamandis, E.P.
Complex formation between human kallikrein 13 and serum protease inhibitors
Clin. Chim. Acta
339
157-167
2004
Homo sapiens
Darling, M.R.; Jackson-Boeters, L.; Daley, T.D.; Diamandis, E.P.
Human kallikrein 13 expression in salivary gland tumors
Int. J. Biol. Markers
21
106-110
2006
Homo sapiens
Scorilas, A.; Borgono, C.A.; Harbeck, N.; Dorn, J.; Schmalfeldt, B.; Schmitt, M.; Diamandis, E.P.
Human kallikrein 13 protein in ovarian cancer cytosols: a new favorable prognostic marker
J. Clin. Oncol.
22
678-685
2004
Homo sapiens
Komatsu, N.; Tsai, B.; Sidiropoulos, M.; Saijoh, K.; Levesque, M.A.; Takehara, K.; Diamandis, E.P.
Quantification of eight tissue kallikreins in the Stratum corneum and sweat
J. Invest. Dermatol.
126
925-929
2006
Homo sapiens
Shaw, J.L.; Diamandis, E.P.
Distribution of 15 human kallikreins in tissues and biological fluids
Clin. Chem.
53
1423-1432
2007
Homo sapiens
Planque, C.; Blechet, C.; Ayadi-Kaddour, A.; Heuze-Vourch, N.; Dumont, P.; Guyetant, S.; Diamandis, E.P.; El Mezni, F.; Courty, Y.
Quantitative RT-PCR analysis and immunohistochemical localization of the kallikrein-related peptidases 13 and 14 in lung
Biol. Chem.
389
781-786
2008
Homo sapiens
Planque, C.; Li, L.; Zheng, Y.; Soosaipillai, A.; Reckamp, K.; Chia, D.; Diamandis, E.P.; Goodglick, L.
A multiparametric serum kallikrein panel for diagnosis of non-small cell lung carcinoma
Clin. Cancer Res.
14
1355-1362
2008
Homo sapiens
Andrade, D.; Assis, D.M.; Santos, J.A.; Alves, F.M.; Hirata, I.Y.; Araujo, M.S.; Blaber, S.I.; Blaber, M.; Juliano, M.A.; Juliano, L.
Substrate specificity of kallikrein-related peptidase 13 activated by salts or glycosaminoglycans and a search for natural substrate candidates
Biochimie
93
1701-1709
2011
Homo sapiens
Ishige, S.; Kasamatsu, A.; Ogoshi, K.; Saito, Y.; Usukura, K.; Yokoe, H.; Kouzu, Y.; Koike, H.; Sakamoto, Y.; Ogawara, K.; Shiiba, M.; Tanzawa, H.; Uzawa, K.
Decreased expression of kallikrein-related peptidase 13: possible contribution to metastasis of human oral cancer
Mol. Carcinog.
53
557-565
2014
Homo sapiens (Q9UKR3)
-
Florou, D.; Mavridis, K.; Scorilas, A.
The kallikrein-related peptidase 13 (KLK13) gene is substantially up-regulated after exposure of gastric cancer cells to antineoplastic agents
Tumour Biol.
33
2069-2078
2012
Homo sapiens (Q9UKR3), Homo sapiens
Gueugnon, F.; Barascu, A.; Mavridis, K.; Petit-Courty, A.; Marchand-Adam, S.; Gissot, V.; Scorilas, A.; Guyetant, S.; Courty, Y.
Kallikrein-related peptidase 13: an independent indicator of favorable prognosis for patients with nonsmall cell lung cancer
Tumor Biol.
36
4979-4986
2015
Homo sapiens (Q9UKR3), Homo sapiens
Muytjens, C.M.J.; Yu, Y.; Diamandis, E.P.
Functional proteomic profiling reveals KLK13 and TMPRSS11D as active proteases in the lower female reproductive tract
F1000Res.
7
1666
2018
Homo sapiens (Q9UKR3), Homo sapiens
Gruba, N.; Bielecka, E.; Wysocka, M.; Wojtysiak, A.; Brzezinska-Bodal, M.; Sychowska, K.; Kalinska, M.; Magoch, M.; Pecak, A.; Falkowski, K.; Wisniewska, M.; Sasiadek, L.; Plaza, K.; Kroll, E.; Pejkovska, A.; Rehders, M.; Brix, K.; Dubin, G.; Kantyka, T.; Potempa, J.; Lesner, A.
Development of chemical tools to monitor human kallikrein 13 (KLK13) activity
Int. J. Mol. Sci.
20
1557
2019
Homo sapiens (Q9UKR3), Homo sapiens
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