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Information on EC 3.4.21.119 - kallikrein 13
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3.4.21.119 kallikrein 13Specify your search results
Word Map
- 3.4.21.119
- kallikreins
- prorenins
-
submandibular
- medicine
-
trypsin-like
-
endoproteolytic
- diagnostics
-
renin-angiotensin
- analysis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
Synonyms
kallikrein 13, prece, mgk-13, prorenin converting enzyme, prorenin-converting enzyme, kallikrein-related peptidase 13, glandular kallikrein 13, gk-13, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kallikrein-related peptidase 13
KLK13
S01.306
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
substrate binding
-
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
enzyme is a serine protease
-
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
enzyme is a serine protease
-
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
enzyme is a serine protease
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
preference for Xaa-Arg-Arg- motif
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Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
active site is located in the cleft between two beta-barrel domains, with an ordered kallikrein loop conformation close to it
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Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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-
-
-
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CAS REGISTRY NUMBER
COMMENTARY
342900-44-1
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9001-01-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
35 kDa pro-interleukin 1beta + H2O
17.5 kDa interleukin 1beta + 20 kDa interleukin 1beta
-
cleavage of Leu113-Leu114 bond
-
-
?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
Abz-MISLMKRPPGFSPFR + SSRI-NH2
-
rapid cleavage
-
-
?
ABZ-Val-Arg-Phe-Arg-5-amino-2-nitrobenzoic acid + H2O
ABZ-Val-Arg-Phe-Arg + 5-amino-2-nitrobenzoic acid
kinetically preferred substrate
-
-
?
benzyloxycarbonyl-Arg-Val-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
-
low activity
-
-
?
benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
benzyloxycarbonyl-Glu-Lys-Lys-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
-
low activity
-
-
?
benzyloxycarbonyl-Leu-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
FSR-amido-4-methylcoumarin + H2O
FSR + 7-amino-4-methylcoumarin
-
fluorogenic trypsin-like peptide substrate
-
-
?
histatin 3 + H2O
DSHAKRHHGYKRKFHEKHHSHRGYR + SNYLYDN
-
i.e. DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN, efficient substrate
-
-
?
mouse prorenin Ren2 + H2O
renin + ?
-
specific cleavage of Lys-Arg bond of mouse prorenin Ren2
-
-
?
myelin basic protein + H2O
?
-
at least two cleavage sites are identified at YGGR-ASDYK and PRHR-DTGI
-
-
?
pyroglutamyl-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
pyroglutamyl-Arg-Tyr-Lys-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
VPR-amido-4-methylcoumarin + H2O
VPR + 7-amino-4-methylcoumarin
-
fluorogenic trypsin-like peptide substrate
-
-
?
polypeptide + H2O
peptides
-
cleavage site at paired basic amino acid residues
-
?
polypeptide + H2O
peptides
-
cleavage site at paired basic amino acid residues
-
?
Prorenin + H2O
Renin + ?
-
cleavage site at paired basic amino acid residues
-
?
Prorenin + H2O
Renin + ?
-
cleavage site at paired basic amino acid residues
-
?
Prorenin + H2O
Renin + ?
-
cleavage site at paired basic amino acid residues
-
?
Prorenin + H2O
Renin + ?
-
cleavage site at paired basic amino acid residues
-
?
Prorenin + H2O
Renin + ?
-
no cleavage of the peptide bond between Arg and Pro
-
?
Prorenin + H2O
Renin + ?
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specifically cleaves the peptide bond on the COOH-side of the Arg residue at the Lys-Arg pair of mouse Ren 2 prorenin, but does not cleave mouse Ren 1 and human prorenins
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?
Prorenin + H2O
Renin + ?
-
murine or human origin substrate from Xenopus oocytes expression system
-
?
Prorenin + H2O
Renin + ?
-
inactive precursor
product is an active enzyme
?
Prorenin + H2O
Renin + ?
-
inactive precursor
product is an active enzyme
?
Prorenin + H2O
Renin + ?
-
inactive precursor
product is an active enzyme
?
additional information
?
-
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enzyme is involved in tissue remodeling and/or tumor invasion and metastasis
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-
?
additional information
?
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KLK13 does not hydrolyze Abz-KLRESKQ-EDDnp, Abz-KLRSEKQ-EDDnp, and AbzeELRSSKQ-EDDnp
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-
?
additional information
?
-
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the S2' subsite of KLK13 does not have restricted specificity but does present preference for the basic amino acid R and does not accepted negative charge
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-
?
additional information
?
-
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glycosylation near the processing site might prevent hydrolysis
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-
?
additional information
?
-
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no substrate: mouse prorenin Ren1, human prorenin
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme is involved in tissue remodeling and/or tumor invasion and metastasis
-
-
?
Prorenin + H2O
Renin + ?
-
inactive precursor
product is an active enzyme
?
Prorenin + H2O
Renin + ?
-
inactive precursor
product is an active enzyme
?
Prorenin + H2O
Renin + ?
-
inactive precursor
product is an active enzyme
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
Na+, K+, Mg2+ and Ca2+ up to 1 M concentration do not activate the enzyme
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
little or no effect by the following protease inhibitors: PMSF, DFP, aprotinin, iodoacetamide, NEM, PCMBS, and E-64; no inhibition by EDTA and pepstatin A
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma of Lung
Epigenetic activation of human kallikrein 13 enhances malignancy of lung adenocarcinoma by promoting N-cadherin expression and laminin degradation.
Breast Neoplasms
Development of Chemical Tools to Monitor Human Kallikrein 13 (KLK13) Activity.
Carcinogenesis
Mucin 16 and kallikrein 13 as potential prognostic factors in colon cancer: Results of an oncological 92-multiplex immunoassay.
Carcinoma
Correction to: Expression of kallikrein-related peptidase 13 is associated with poor prognosis in esophageal squamous cell carcinoma.
Carcinoma
Decreased expression of kallikrein-related peptidase 13: Possible contribution to metastasis of human oral cancer.
Carcinoma
Expression of kallikrein-related peptidase 13 is associated with poor prognosis in esophageal squamous cell carcinoma.
Carcinoma
Kallikrein-related peptidase 13 (KLK13) gene expressional status contributes significantly in the prognosis of primary gastric carcinomas.
Carcinoma, Non-Small-Cell Lung
Kallikrein-related peptidase 13: an independent indicator of favorable prognosis for patients with nonsmall cell lung cancer.
Carcinoma, Ovarian Epithelial
KLK6 and KLK13 predict tumor recurrence in epithelial ovarian carcinoma.
Colonic Neoplasms
Mucin 16 and kallikrein 13 as potential prognostic factors in colon cancer: Results of an oncological 92-multiplex immunoassay.
Diabetic Cardiomyopathies
Role of p90 ribosomal S6 kinase-mediated prorenin-converting enzyme in ischemic and diabetic myocardium.
Dry Eye Syndromes
Autoantibodies Contribute to the Immunopathogenesis of Experimental Dry Eye Disease.
Esophageal Squamous Cell Carcinoma
Correction to: Expression of kallikrein-related peptidase 13 is associated with poor prognosis in esophageal squamous cell carcinoma.
Esophageal Squamous Cell Carcinoma
Expression of kallikrein-related peptidase 13 is associated with poor prognosis in esophageal squamous cell carcinoma.
Hyperglycemia
Role of p90 ribosomal S6 kinase-mediated prorenin-converting enzyme in ischemic and diabetic myocardium.
Hypertension
Renin activity and angiotensin I concentration in genetically selective inbred line of hypertensive mice.
Infections
Kallikrein 13 serves as a priming protease during infection by the human coronavirus HKU1.
Lung Neoplasms
Kallikrein-related peptidase 13: an independent indicator of favorable prognosis for patients with nonsmall cell lung cancer.
Mouth Neoplasms
Decreased expression of kallikrein-related peptidase 13: Possible contribution to metastasis of human oral cancer.
Neoplasm Metastasis
Decreased expression of kallikrein-related peptidase 13: Possible contribution to metastasis of human oral cancer.
Neoplasms
Epigenetic activation of human kallikrein 13 enhances malignancy of lung adenocarcinoma by promoting N-cadherin expression and laminin degradation.
Neoplasms
Expression of kallikrein-related peptidase 13 is associated with poor prognosis in esophageal squamous cell carcinoma.
Ovarian Neoplasms
Human kallikrein 13 protein in ovarian cancer cytosols: a new favorable prognostic marker.
Squamous Cell Carcinoma of Head and Neck
Decreased expression of kallikrein-related peptidase 13: Possible contribution to metastasis of human oral cancer.
Stomach Neoplasms
The kallikrein-related peptidase 13 (KLK13) gene is substantially up-regulated after exposure of gastric cancer cells to antineoplastic agents.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0053
Abz-KLKSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.031
Abz-KLKSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.0012
Abz-KLRSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.0022
Abz-KLRSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.1
Abz-KLKSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
0.5
Abz-KLKSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
3.2
Abz-KLRSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
3.4
Abz-KLRSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
16
Abz-KLKSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
19
Abz-KLKSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
1455
Abz-KLRSSKQ-EDDnp
-
in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
2834
Abz-KLRSSKQ-EDDnp
-
in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0011
-
purified enzyme, substrate benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
expression of the KLK13 and KLK14 genes are examined at the mRNA and protein levels in a cohort of 57 patients with non-small-cell lung cancer. The mRNA levels, assessed by real-time RT-PCR, are significantly different in malignant tissues compared to adjacent non-malignant tissues. KLK13 and KLK14 mRNA overexpression in tumors is associated with a positive nodal status in multivariate analysis
brenda
kallikrein-related peptidase 13 is downregulated in oral squamous cell carcinoma cell lines, primary tumors and established cell lines
brenda
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the clinical value of 11 members of the tissue kallikrein family as potential biomarkers for lung cancer diagnosis are analyzed: Serum specimens from 51 patients with non-small cell lung cancer and from50 healthy volunteers are collected. Samples are analyzed for 11 kallikreins (KLK1, KLK4-8, and KLK10-14) by specific ELISA. compared with sera from normal subjects, sera of patients with non-small cell lung cancer have lower levels of KLK5, KLK7, KLK8, KLK10, and KLK12, and higher levels of KLK11, KLK13, and KLK14. KLK11, KLK12, KLK13, and KLK14 are associated with higher risk of non-small cell lung cancer as determined by univariate analysis and confirmed by multivariate analysis
brenda
additional information
-
quantification of eight different tissue kallikreins. Enzyme is present at significantly lower concentrations in females than in males
brenda
-
hK13 is present at significantly lower concentrations in females than in males
brenda
-
enzyme regulation is parallel and similar to that of renin
brenda
-
quantification of eight different tissue kallikreins. Enzyme is detected only in the eccrine sweat glands regardless of gender
brenda
-
from the face with highest amounts, in the eccrine sweat glands regardless of gender
brenda