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Information on EC 3.4.21.115 - infectious pancreatic necrosis birnavirus Vp4 peptidase and Organism(s) Blotched snakehead virus and UniProt Accession Q8AZM0

for references in articles please use BRENDA:EC3.4.21.115
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This record set is specific for:
Blotched snakehead virus
UNIPROT: Q8AZM0 not found.
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Word Map
The taxonomic range for the selected organisms is: Blotched snakehead virus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
cleaves the (Ser/Thr)-Xaa-Ala-/-(Ser/Ala)-Gly motif in the polyprotein NH2-pVP2-VP4-VP3-COOH of infectious pancreatic necrosis virus at the pVP2-VP4 and VP4-VP3 junctions
Synonyms
ns protease, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
blotched snakehead birnavirus endopeptidase
-
-
equine arterivirus serine peptidase
-
-
-
-
infectious pancreatic necrosis birnavirus Vp4 protease
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
hydrolysis of peptide bond
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
330588-95-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
polyprotein of BSNV + H2O
pVP2 + VP3 + VP4
show the reaction diagram
-
-
-
?
polyprotein of BSNV + H2O
pVP2 + VP3 + VP4
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polyprotein of BSNV + H2O
pVP2 + VP3 + VP4
show the reaction diagram
-
-
-
?
polyprotein of BSNV + H2O
pVP2 + VP3 + VP4
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
POLS_BSNV
1069
0
115494
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28500
-
SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a truncated variant with 18 residues removed from the C-terminal
a truncated variant with 18 residues removed from the C-terminal
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
P774Z
18 residues removed from the C-terminus
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation and size-exclusion chromatography
two chromatography steps to homogeneity
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, J.; Feldman, A.R.; Delmas, B.; Paetzel, M.
Expression, purification and crystallization of a birnavirus-encoded protease, VP4, from blotched snakehead virus (BSNV)
Acta Crystallogr. Sect. F
F62
353-356
2006
Blotched snakehead virus
Manually annotated by BRENDA team
Gorbalenya, A.E.; Mundt, E.
Birnavirus VP4 processing endopeptidase
Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press
2
1995-1998
2004
Blotched snakehead virus, Drosophila X virus, Infectious bursal disease virus, infectious pancreatic necrosis virus
-
Manually annotated by BRENDA team
Feldman, A.R.; Lee, J.; Delmas, B.; Paetzel, M.
Crystal structure of a novel viral protease with a serine/lysine catalytic dyad mechanism
J. Mol. Biol.
358
1378-1389
2006
Blotched snakehead virus (Q8AZM0)
Manually annotated by BRENDA team