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Information on EC 3.4.21.101 - xanthomonalisin and Organism(s) Xanthomonas sp. and UniProt Accession Q60106

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.101 xanthomonalisin
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Select one or more organisms in this record: ?
This record set is specific for:
Xanthomonas sp.
UNIPROT: Q60106 not found.
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The taxonomic range for the selected organisms is: Xanthomonas sp.
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide
cleavage of casein
Synonyms
pseudomonas carboxyl proteinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sedolisin-B
-
Xanthomonas serine-carboxyl peptidase
-
Carboxyl proteinase
-
-
-
-
PCP
-
-
-
-
Proteinase, Xanthomonas aspartic
-
-
-
-
sedolisin-B
-
-
xanthomonapepsin
Xanthomonas aspartic proteinase
-
-
-
-
Xanthomonas serine-carboxyl proteinase
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
113356-29-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Acid-denatured hemoglobin + H2O
?
show the reaction diagram
-
-
-
?
casein + H2O
?
show the reaction diagram
-
-
-
?
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
pH 3.5, 37°C
-
-
?
[protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine + H2O
?
show the reaction diagram
-
-
-
?
Acid-denatured hemoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
casein + H2O
?
show the reaction diagram
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Leu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Phe-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Tyr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
additional information
?
-
-
the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has an Ala replacement at the P3 position and preferres such charged amino acid residue as Glu, Asp, Arg, or Lys at the P2' position
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
Ca2+-binding site
additional information
-
no inhibition or activation by divalent cations
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
isovaleryl-tyrosyl-leucyl-tyrosinal
tyrostatin
1,2-epoxy-3-(4-nitrophenoxy)propane
-
-
Microbial alkaline protease inhibitor alpha
-
weak
-
PCMB
-
slight
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0036
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
pH3.5, 37°C
0.00605
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0058 - 0.0188
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
0.00932
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0115
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0139
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.013
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.012
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.005
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0135
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00531
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0026
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0126
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0174
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
-
pH 3.5, 37°C
0.00763
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
-
pH 3.5, 37°C
0.0063
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
-
pH 3.5, 37°C
0.00995
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
-
pH 3.5, 37°C
0.00824
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
-
pH 3.5, 37°C
0.0106
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00746
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
-
pH 3.5, 37°C
0.00982
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
-
pH 3.5, 37°C
0.00944
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
-
pH 3.5, 37°C
0.00532
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
-
pH 3.5, 37°C
0.0183
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
-
pH 3.5, 37°C
0.0213
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
-
pH 3.5, 37°C
0.0217
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
-
pH 3.5, 37°C
0.00977
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
-
pH 3.5, 37°C
0.0283
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
-
pH 3.5, 37°C
0.0158
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
-
pH 3.5, 37°C
0.0164
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
-
pH 3.5, 37°C
0.0138
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
-
pH 3.5, 37°C
0.0101
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00716
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00214
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0173
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0662
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00782
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00523
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00451
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00445
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0114
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0183
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0105
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0039
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0071
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00224
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00995
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
52.2
1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol
pH3.5, 37°C
0.037 - 79.4
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.3 - 68.4
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
105
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
38.3
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
18.1
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
2.2 - 73.9
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
34 - 62.4
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
60.5
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
43.3
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.8 - 35.7
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
18.6
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
48.8
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
1.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
-
pH 3.5, 37°C
24.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
-
pH 3.5, 37°C
15.5
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
-
pH 3.5, 37°C
101
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
-
pH 3.5, 37°C
27.3
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
-
pH 3.5, 37°C
102
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
26.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
-
pH 3.5, 37°C
1.6 - 71.3
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
27.6
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
-
pH 3.5, 37°C
30.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
-
pH 3.5, 37°C
32.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
-
pH 3.5, 37°C
20 - 87.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
189
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
-
pH 3.5, 37°C
16.1
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
-
pH 3.5, 37°C
31
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
-
pH 3.5, 37°C
43.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
-
pH 3.5, 37°C
40.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
-
pH 3.5, 37°C
0.04 - 8.57
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
16.9 - 23.1
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
1.1 - 19.4
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
16.8
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.97 - 16.6
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
39.8
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
6 - 57.9
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
56.8
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
30.5
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
15.9 - 26.9
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
15.3
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
96.4
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
2.6 - 57.3
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
3.4 - 36.7
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
37
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
8.45
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
28.1
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000021
isovaleryl-tyrosyl-leucyl-tyrosinal
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.7
for casein digestion
2.7
-
casein, acid-denatured hemoglobin
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
T-22
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
2 * 41000, SDS-PAGE
84000
recombinant protein from Escherichia coli
40000
-
gel filtration
41000
-
1 * 41000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 41000, SDS-PAGE
monomer
-
1 * 41000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
N-terminal propeptide and C-terminal propeptide
additional information
-
contains less than 0.05% carbohydrate
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D348A
-
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
DELTA192
-
pro-region mutant enzyme
S169A
-
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
S79A
-
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 5.3
-
37°C, 20 h, stable
137235
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
pH 3.5-5.3, 20 h, stable
50
-
pH 4.8, 15 min, stable below
60
-
pH 4.8, 15 min, complete loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant mutant enzymes S79A, S169A and D348A
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Oda, K.; Nakazima, T.; Terashita, T.; Suzuki, K.i.; Murao, S.
Purification and properties of an S-PI(pepstatin Ac)-insensitive carboxyl proteinase from a Xanthomonas sp. Bacterium
Agric. Biol. Chem.
51
3073-3080
1987
Xanthomonas sp.
-
Manually annotated by BRENDA team
Wlodawer, A.; Li, M.; Gustchina, A.; Oyama, H.; Dunn, B.M.; Oda, K.
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases
Acta Biochim. Pol.
50
81-102
2003
Xanthomonas sp.
Manually annotated by BRENDA team
Narutaki, S.; Dunn, B.M.; Oda, K.
Subsite preferences of pepstatin-insensitive carboxyl proteinases from bacteria
J. Biochem.
125
75-81
1999
Xanthomonas sp., Xanthomonas sp. T-22
Manually annotated by BRENDA team
Oyama, H.; Abe, S.I.; Ushiyama, S.; Takahashi, S.; Oda, K.
Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis
J. Biol. Chem.
274
27815-27822
1999
Xanthomonas sp.
Manually annotated by BRENDA team
Oda, K.
Sedolisin-B
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1888-1889
2004
Xanthomonas sp. (Q60106), Xanthomonas sp. T-22 (Q60106)
-
Manually annotated by BRENDA team