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Information on EC 3.4.21.10 - acrosin and Organism(s) Meleagris gallopavo and UniProt Accession Q2UVH8

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.10 acrosin
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This record set is specific for:
Meleagris gallopavo
UNIPROT: Q2UVH8 not found.
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The taxonomic range for the selected organisms is: Meleagris gallopavo
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
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preferential cleavage: Arg-/-, Lys-/-
Synonyms
acrosin, sperm acrosin, beta-acrosin, alpha-acrosin, acrosomal protease, acrosomal proteinase, acrosin amidase, acrosin ii, psi-acrosin, acrosin i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53 kDa fucose-binding protein
-
-
-
-
acrosin amidase
-
-
-
-
acrosin I
-
isoform
acrosin II
-
isoform
acrosomal protease
-
-
-
-
acrosomal proteinase
-
-
-
-
acrozonase
-
-
-
-
alpha-acrosin
-
-
-
-
beta-acrosin
-
-
-
-
proteinase, acrosomal
-
-
-
-
psi-acrosin
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9068-57-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-arginine p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
proacrosin + H2O
acrosin + ?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
proacrosin + H2O
acrosin + ?
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Kazal seminal plasma inhibitor
-
-
4-nitrophenyl 4-guanidinobenzoate
-
-
Aprotinin
-
-
benzamidine
-
-
ovomucoid
-
-
-
p-aminobenzamidine
-
-
Trypsin inhibitor
-
soybean
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
isoelectric focusing
7.3
mature protein, calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ACRO_MELGA
346
0
37950
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
x * 30000, active enzyme, SDS-PAGE
30874
x * 30874, mass spectrometry
360517
x * 360517, mature protein, calculated from amino acid sequence
30000
-
x * 30000, isoform acrosin I, SDS-PAGE
30869
-
x * 30869, mass spectrometry
41000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
one potential N-glycosylation site is present at Asn-128 and three potential O-glycosylation sites are present at Thr-184, Thr-300 and Thr-312
glycoprotein
-
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no differences are found in the enzyme activity of sperm suspension in fresh and frozen-thawed semen
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Superdex 200 gel filtration
BioSep-SEC-S2000 column chromatography, BioBasic 8 column chromatography, and Superdex 200 gel filtration
-
BioSep-SEC-S2000 gel filtration, BioBasic 8 column chromatography, and Superdex 200 gel filtration
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Richardson, M.E.; Korn, N.; Bodine, A.B.; Thurston, R.J.
Research note: kinetic and inhibition studies with turkey acrosin
Poult. Sci.
71
1789-1793
1992
Meleagris gallopavo
Manually annotated by BRENDA team
Slowinska, M.; Olczak, M.; Liszewska, E.; Watorek, W.; Ciereszko, A.
Isolation, characterization and cDNA sequencing of acrosin from turkey spermatozoa
Comp. Biochem. Physiol. B
157
127-136
2010
Meleagris gallopavo (Q2UVH8), Meleagris gallopavo
Manually annotated by BRENDA team
Slowinska, M.; Ciereszko, A.
Identification of the second form of acrosin in Turkey spermatozoa
Reprod. Domest. Anim.
47
849-855
2012
Meleagris gallopavo
Manually annotated by BRENDA team
Slowinska, M.; Liszewska, E.; Dietrich, G.J.; Ciereszko, A.
Characterization of proacrosin/acrosin system after liquid storage and cryopreservation of turkey semen (Meleagris gallopavo)
Theriogenology
78
1065-1077
2012
Meleagris gallopavo
Manually annotated by BRENDA team