Information on EC 3.4.19.7 - N-Formylmethionyl-peptidase

for references in articles please use BRENDA:EC3.4.19.7
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The expected taxonomic range for this enzyme is: Rattus norvegicus

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EC NUMBER
COMMENTARY hide
3.4.19.7
-
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RECOMMENDED NAME
GeneOntology No.
N-Formylmethionyl-peptidase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal, formyl-methionyl residue from a polypeptide
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
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CAS REGISTRY NUMBER
COMMENTARY hide
76106-80-4
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Acetyl-Met-Leu + H2O
?
show the reaction diagram
-
-
-
-
-
Acetyl-Met-Leu-Phe + H2O
?
show the reaction diagram
-
-
-
-
-
Formylnorleucine-Leu-Phe + H2O
?
show the reaction diagram
-
-
-
-
-
N-Formyl-Met 2-naphthylamide + H2O
N-Formyl-Met + 2-naphthylamine
show the reaction diagram
-
-
-
-
-
N-Formyl-Met-Leu + H2O
N-Formyl-Met + Leu
show the reaction diagram
-
-
-
-
-
N-Formyl-Met-Leu-Phe + H2O
N-Formyl-Met + Leu-Phe
show the reaction diagram
-
-
-
-
N-Formyl-Met-Leu-Tyr + H2O
N-Formyl-Met + Leu-Tyr
show the reaction diagram
-
-
-
-
N-Formyl-Met-Phe + H2O
N-Formyl-Met + Phe
show the reaction diagram
-
-
-
-
N-Formyl-Met-Trp + H2O
N-Formyl-Met + Trp
show the reaction diagram
-
-
-
-
N-Formyl-Met-Val + H2O
N-Formyl-Met + Val
show the reaction diagram
-
-
-
-
N-Formylmethionyl oligopeptides + H2O
?
show the reaction diagram
-
the presence of this enzyme in intestinal mucosa can prevent absorption of intact bioactive potent inflammatory N-formylmethionyl oligopeptides produced by commensal bacteria in the gut lumen
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-
-
Natural formylmethionine oligopeptide from E. coli culture supernatants + H2O
?
show the reaction diagram
-
loss of bioactivity and immuno-reactivity
-
-
-
additional information
?
-
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highly specific for N-formyl-Met peptides, will not cleave methionyl peptides or N-formyl derivatives of amino acids other than methionine
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-Formylmethionyl oligopeptides + H2O
?
show the reaction diagram
-
the presence of this enzyme in intestinal mucosa can prevent absorption of intact bioactive potent inflammatory N-formylmethionyl oligopeptides produced by commensal bacteria in the gut lumen
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Benzyl succinate
-
-
Heavy metal ions
-
-
-
phenylmethanesulfonyl fluoride
-
weak
Zn2+
-
moderate
additional information
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
Acetyl-Met-Leu
-
-
0.16
Acetyl-Met-Leu-Phe
-
-
0.23
Formylnorleucine-Leu-Phe
-
-
0.2 - 1.7
N-Formyl-Met 2-naphthylamide
0.03 - 0.04
N-Formyl-Met-Leu
0.18
N-Formyl-Met-Leu-Phe
-
-
0.22
N-Formyl-Met-Leu-Tyr
-
-
0.63
N-Formyl-Met-Phe
-
-
0.71
N-Formyl-Met-Trp
-
-
0.06
N-Formyl-Met-Val
-
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.45
Acetyl-Met-Leu
-
-
27.1
Acetyl-Met-Leu-Phe
-
-
76.4 - 230
N-Formyl-Met 2-naphthylamide
5.17
N-Formyl-Met-Leu
-
-
12.3
N-Formyl-Met-Leu-Phe
-
-
15.5
N-Formyl-Met-Leu-Tyr
-
-
70.2
N-Formyl-Met-Phe
-
-
31.3
N-Formyl-Met-Trp
-
-
5.94
N-Formyl-Met-Val
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
6: about 40% of activity maximum, 10: about 50% of activity maximum
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
lowest level in oesophagus and stomach and a shallow diminishing gradient from proximal small intestine to distal colon and rectum
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72000
-
4 * 72000, SDS-PAGE
290000
-
rat, gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 72000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
intestine
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LINKS TO OTHER DATABASES (specific for EC-Number 3.4.19.7)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)