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Information on EC 3.4.19.12 - ubiquitinyl hydrolase 1 and Organism(s) Saccharomyces cerevisiae and UniProt Accession P35127

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.12 ubiquitinyl hydrolase 1
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P35127 not found.
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
uch-l1, uchl1, ataxin-3, deubiquitinating enzyme, pgp 9.5, usp22, pgp9.5, plpro, otub1, isopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
yeast ubiquitin hydrolase
-
AD-019
-
-
-
-
CGI-70
-
-
-
-
Doa4 ubiquitin hydrolase
-
-
Doa4p ubiquitin isopeptidase
-
-
esterase, ubiquitin thiol-
-
-
-
-
hydrolase, ubiquitin carboxyl-terminal
-
-
-
-
hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch)
-
-
-
-
isopeptidase
-
-
-
-
isopeptidase T
-
-
-
-
Neuron cytoplasmic protein 9.5
-
-
-
-
PGP 9.5
-
-
-
-
PGP9.5
-
-
-
-
ubiquitin C-terminal hydrolase
-
-
-
-
ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch)
-
-
-
-
ubiquitin C-terminal hydrolase 1
-
-
Ubiquitin C-terminal hydrolase UCH37
-
-
-
-
ubiquitin carboxy-terminal hydrolase
-
-
-
-
ubiquitin carboxyl-terminal hydrolase
-
-
-
-
Ubiquitin thiolesterase
-
-
-
-
Ubiquitin thiolesterase L1
-
-
-
-
Ubiquitin thiolesterase L3
-
-
-
-
Ubiquitin thiolesterase L4
-
-
-
-
Ubiquitin thiolesterase L5
-
-
-
-
ubiquitin-carboxyl-terminal hydrolase PGP-9.5
-
-
-
-
UCH-1
-
-
-
-
UCH-L1
-
-
-
-
UCH-L2
-
-
-
-
UCH-L3
-
-
-
-
UCH-L4
-
-
-
-
UCH-L5
-
-
-
-
yeast ubiquitin hydrolase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
189642-63-5
-
86480-67-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ubiquitin-piscidin 1 + H2O
ubiquitin + piscidin 1
show the reaction diagram
-
-
-
?
(His)8-Ub-CTAPIII + H2O
(His)8-Ub + CTAPII
show the reaction diagram
-
Ub i.e ubiquitin, CTAPII i.e. connective tissue-activating peptide
-
?
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
show the reaction diagram
-
i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds
-
?
linear ubiquitin-beta-galactosidase + H2O
ubiquitin + protein
show the reaction diagram
-
recombinant fusion protein co-expressed with the enzyme in Escherichia coli
-
?
ubiquitin + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
show the reaction diagram
-
carboxyl extension protein of 38 amino acids
-
-
?
ubiquitin-Cys + H2O
ubiquitin + Cys
show the reaction diagram
-
-
-
?
ubiquitin-Cys-Cys + H2O
ubiquitin + Cys-Cys
show the reaction diagram
-
-
-
?
ubiquitin-Fur4p-conjugate + H2O
ubiquitin + Fur4p
show the reaction diagram
-
ubiquitin is bound to the Fur4p protein via its Lys63
-
?
ubiquitin-protein + H2O
ubiquitin + protein
show the reaction diagram
Z-Arg-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ubiquitin-protein + H2O
ubiquitin + protein
show the reaction diagram
additional information
?
-
-
enzyme regulation
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
divalent cations
-
most divalent cations, except Mg2+ and Ca2+ are inhibitory
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
-
gel filtration, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C571S
-
is unable to maintain normal ubiquitin levels. In doa4delta cells expressing C571S, CPS is missorted to the limiting membrane of the vacuole
doa4delta
-
defect in endosomal localization
W782R
-
is solely cytoplasmic, defect in endosomal localization, Bro1-Doa4 interaction is disrupted
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
H6YUH1 shows 3.2 and 1.4 h of half lives at 4°C and 52°C, respectively. Immobilized YUH1 in 50% glycerol solution at -20°C results in 52% of decrease in specific activity for 7 days, corresponding to a 2.7fold increase compared with that of free YUH1 incubated in the same solution at 4°C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 20 mM sodium phosphate buffer, pH 7.4, 100% glycerol
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by two purification steps, gel filtration
by Ni-NTA affinity chromatography, to more than 90% homogeneity
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
YUH gene ligated with the pET-23d vector, expressed in Escherichia coli Rosetta(DE3)pLysS
co-expression of Doa4p with cyk-3 of Caenorhabditis elegans and ubiquitin-beta-galactosidase fusion protein in Escherichia coli
-
two plasmids with two types of YUH1 with the 6 histidine tag at the N-terminus (H6YUH1) or C-terminus (YUH1H6) expressed in Escherichia coli DH5alpha
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
used as molecular scissors for releasing a peptide or protein product
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mildner, A.M.; Paddock, D.J.; LeCureux, L.W.; Leone, J.W.; Anderson, D.C.; Tomasselli, A.G.; Heinrikson, R.L.
Production of chemokines CTAPIII and NAP/2 by digestion of recombinant ubiquitin-CTAPII with yeast ubiquitin C-terminal hydrolase and human immunodeficiency virus protease
Protein Expr. Purif.
16
347-354
1999
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Liu, C.C.; Miller, H.I.; Kohr, W.J.; Silber, J.I.
Purification of a ubiquitin protein peptidase from yeast with efficient in vitro assays
J. Biol. Chem.
264
20331-20338
1989
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Matsui, S.; Sandberg, A.A.; Negoro, S.; Seon, B.K.; Goldstein, G.
Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds
Proc. Natl. Acad. Sci. USA
79
1535-1539
1982
amphibia, Saccharomyces cerevisiae, Gallus gallus, Cricetulus griseus, eukaryota, Mammalia, no activity in Escherichia coli
Manually annotated by BRENDA team
Dupre, S.; Haguenauer-Tsapis, R.
Deubiquitination step in the endocytic pathway of yeast plasma membrane proteins: crucial role of Doa4p ubiquitin isopeptidase
Mol. Cell. Biol.
21
4482-4494
2001
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kaitna, S.; Schnabel, H.; Schnabel, R.; Hyman, A.A.; Glotzer, M.
A ubiquitin C-terminal hydrolase is required to maintain osmotic balance and execute actin-dependent processes in the early C. elegans embryo
J. Cell Sci.
115
2293-2302
2002
Saccharomyces cerevisiae, Caenorhabditis elegans
Manually annotated by BRENDA team
Nikko, E.; Andre, B.
Split-ubiquitin two-hybrid assay to analyze protein-protein interactions at the endosome: application to Saccharomyces cerevisiae Bro1 interacting with ESCRT complexes, the Doa4 ubiquitin hydrolase, and the Rsp5 ubiquitin ligase
Eukaryot. Cell
6
1266-1277
2007
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Moon, W.J.; Hwang, D.K.; Park, E.J.; Kim, Y.M.; Chae, Y.K.
Recombinant expression, isotope labeling, refolding, and purification of an antimicrobial peptide, piscidin
Protein Expr. Purif.
51
141-146
2007
Saccharomyces cerevisiae (P35127), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Yu, H.A.; Kim, S.G.; Kim, E.J.; Lee, W.J.; Kim, D.O.; Park, K.; Park, Y.C.; Seo, J.H.
Characterization of ubiquitin C-terminal hydrolase 1 (YUH1) from Saccharomyces cerevisiae expressed in recombinant Escherichia coli
Protein Expr. Purif.
56
20-26
2007
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Nikko, E.; Andre, B.
Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway
Traffic
8
566-581
2007
Saccharomyces cerevisiae
Manually annotated by BRENDA team