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Information on EC 3.4.17.4 - Gly-Xaa carboxypeptidase
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EC Tree
3.4.17.4 Gly-Xaa carboxypeptidaseSpecify your search results
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
carboxypeptidase a, carboxypeptidase S, CPS1, Cps1p, EC 3.4.12.8, EC 3.4.17.9, EC 3.4.2.3, GLY-X carboxypeptidase, glycine carboxypeptidase, YSCS, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
carboxypeptidase a
-
-
-
-
carboxypeptidase S
EC 3.4.12.8
-
-
formerly
-
EC 3.4.17.9
-
-
formerly
-
EC 3.4.2.3
-
-
formerly
-
GLY-X carboxypeptidase
-
-
-
-
glycine carboxypeptidase
-
-
-
-
YSCS
-
-
-
-
carboxypeptidase S
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Release of a C-terminal amino acid from a peptide in which glycine is the penultimate amino acid, e.g. Z-Gly-/-Leu
from yeast. In peptidase family M20, glutamate carboxypeptidase family
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
-
-
exopeptidase, C-terminus, amino acid
-
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CAS REGISTRY NUMBER
COMMENTARY
9025-25-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe
benzoyl-Gly-Gly + H2O
?
-
7.2% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzoyl-Gly-Lys + H2O
?
-
2% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzyloxycarbonyl-Ala-Arg(NO2) + H2O
benzyloxycarbonyl-Ala + nitroarginine
-
21% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
Benzyloxycarbonyl-Ala-Phe + H2O
Benzyloxycarbonyl-Ala + Phe
-
10% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
-
1.1% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzyloxycarbonyl-Gly-Glu + H2O
benzyloxycarbonyl-Gly + Glu
-
31% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzyloxycarbonyl-Gly-Gly + H2O
benzyloxycarbonyl-Gly + Gly
-
13% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
Benzyloxycarbonyl-Gly-Leu + H2O
Benzyloxycarbonyl-Gly + Leu
-
best substrate
-
-
?
Benzyloxycarbonyl-Gly-Phe + H2O
Benzyloxycarbonyl-Gly + Phe
-
31% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzyloxycarbonyl-Phe-Leu + H2O
benzyloxycarbonyl-Phe + Leu
-
1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzyloxycarbonyl-Phe-Met + H2O
benzyloxycarbonyl-Phe + Met
-
1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
benzyloxycarbonyl-Phe-Phe + H2O
benzyloxycarbonyl-Phe + Phe
-
1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
dibenzyloxycarbonyl-Lys-Leu + H2O
dibenzyloxycarbonyl-Lys + Leu
-
5.2% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
Gly-Leu-Gly + H2O
?
-
3% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
Gly-Leu-Tyr + H2O
?
-
12% of the activity compared to benzyloxycarbonyl-Gly-Leu
-
-
?
additional information
?
-
-
no hydrolysis of benzyloxycarbonyl-Leu-Leu, benzyloxycarbonyl-Leu-Phe, dibenzyloxycarbonyl-Lys-Phe, benzyloxycarbonyl-Phe-Ser, benzyloxycarbonyl-His-Leu, benzyloxycarbonyl-Pro-Try, benzyloxycarbonyl-Gly-Gly-Leu, benzyloxycarbonyl-Gly-Gly-Phe, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, benzyloxycarbonyl-Ala-Phe-NH2, benzyloxycarbonyl-Phe-Leu-NH2, benzyloxycarbonyl-Phe-Leu-NH2 and Gly-Leu
-
-
?
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe
-
-
enzyme assay is based on the detection of the product using HPLC to separate it from the substrate
?
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe
-
-
enzyme assay is based on the detection of the product using HPLC to separate it from the substrate
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 62%, inhibits the enzyme at higher concentrations, 10 mM
Mn2+
Zn2+
Mn2+
-
MnCl2, 0.1 mM and 1 mM, 106.3% and 104.2% compared to a control without additives
Zn2+
-
ZnSO4, 0.1 mM and 1 mM, 97.6% and 69.6% compared to a control without additives
Zn2+
-
can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 88%, inhibits the enzyme at higher concentrations, 10 mM
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
p-chloromercuribenzoate
-
irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol
1,10-phenanthroline
-
0.1 mM and 1 mM, 95.6% and 74.7% compared to a control without additives
1,10-phenanthroline
-
Ki: 0.035 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+
diisopropylphosphorofluoridate
-
0.1 mM and 1 mM, 32.3% and 24.3% compared to a control without additives
EDTA
-
Ki: 0.013 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+, Zn2+ and Co2+, at a concentration of 0.1 mM, are most effecient with 88% and 62% reactivation
iodoacetate
-
0.1 mM and 1 mM, 110.5% and 94.8% compared to a control without additives
iodoacetate
-
40% inhibition at a concentration of 5 mM, irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no effect by addition of FAD, FMN, NADPH, NADP+, NADH, NAD+
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.8 - 5.4
-
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly as substrate, 50 mM sodium acetate buffer
6 - 6.2
-
benzyloxycarbonyl-Gly-Leu as substrate, in presence of 0.5 M NaCl
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.2 - 6.5
-
pH 4.2: 28% of the maximal activity, pH 6.5: 43% of the maximal activity
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
integral membrane protein, destined for the vacuolar lumen
brenda
-
sorting of integral membrane protein Cps1p into the luminal vesicles of multivesicular bodies
brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CBPS_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
576
1
64597
Swiss-Prot
Secretory Pathway (Reliability: 5)
K0KS81_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
570
1
63688
TrEMBL
other Location (Reliability: 4)
A3GGI9_PICST
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
582
1
65092
TrEMBL
other Location (Reliability: 5)
A0A6B9LT00_9BASI
393
0
41378
TrEMBL
Secretory Pathway (Reliability: 1)
K0KHC4_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
579
1
65588
TrEMBL
other Location (Reliability: 2)
A0A0H3C6J6_CAUVN
Caulobacter vibrioides (strain NA1000 / CB15N)
492
1
51456
TrEMBL
-
A0A0B7FKE4_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
610
1
66142
TrEMBL
other Location (Reliability: 3)
F0RS74_SPHGB
Sphaerochaeta globosa (strain ATCC BAA-1886 / DSM 22777 / Buddy)
477
1
52654
TrEMBL
-
A0A6B9LU01_9BASI
393
0
41441
TrEMBL
Secretory Pathway (Reliability: 1)
A0A0M3R844_9ROSI
499
1
52927
TrEMBL
Secretory Pathway (Reliability: 1)
W1Q877_OGAPD
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)
552
1
62060
TrEMBL
Secretory Pathway (Reliability: 1)
F4GKR1_SPHCD
Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1)
489
1
54484
TrEMBL
-
B8MMJ9_TALSN
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006)
553
0
61333
TrEMBL
Secretory Pathway (Reliability: 3)
A0A6B9M5N4_9BASI
393
0
41323
TrEMBL
Secretory Pathway (Reliability: 1)
A0A0F4YI06_TALEM
546
0
60037
TrEMBL
Mitochondrion (Reliability: 5)
Q8IKI0_PLAF7
385
0
44254
TrEMBL
Secretory Pathway (Reliability: 3)
A0A2P2C7T6_9ZZZZ
453
0
48089
TrEMBL
other Location (Reliability: 3)
A0A1D8NNT8_YARLL
580
0
64198
TrEMBL
other Location (Reliability: 3)
A0A6B9M3P6_9BASI
393
0
41406
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9M5H7_9BASI
393
0
41414
TrEMBL
Secretory Pathway (Reliability: 1)
M5BQN3_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
443
0
48753
TrEMBL
other Location (Reliability: 4)
K0KVP2_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
571
1
63917
TrEMBL
Mitochondrion (Reliability: 5)
A0A097NUZ4_VITVI
357
1
39081
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9LUA7_9BASI
393
0
41418
TrEMBL
Secretory Pathway (Reliability: 1)
A0A060S072_PLARE
385
0
44138
TrEMBL
Secretory Pathway (Reliability: 3)
A0A6B9LT21_9BASI
393
0
41410
TrEMBL
Secretory Pathway (Reliability: 1)
A0A1J1GU63_PLAGA
385
0
44011
TrEMBL
Secretory Pathway (Reliability: 2)
G2SHZ7_RHOMG
Rhodothermus marinus (strain SG0.5JP17-172)
495
1
53447
TrEMBL
-
Q6NCQ7_RHOPA
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
493
1
53821
TrEMBL
-
A2QF94_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
578
0
63641
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9M5J7_9BASI
393
0
41415
TrEMBL
Secretory Pathway (Reliability: 1)
B7Q1Z4_IXOSC
509
1
56312
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9M1D0_9BASI
393
0
41410
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9LZL9_9BASI
393
0
41505
TrEMBL
Secretory Pathway (Reliability: 1)
A0A3G2S2N9_9BASI
639
0
70914
TrEMBL
Mitochondrion (Reliability: 5)
A0A6B9M1E3_9BASI
393
0
41422
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9LT34_9BASI
393
0
41422
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9LZN3_9BASI
393
0
41396
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9LUM5_9BASI
393
0
41351
TrEMBL
Secretory Pathway (Reliability: 1)
M5BJ38_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
545
0
59303
TrEMBL
other Location (Reliability: 1)
A0A6B9LUC9_9BASI
393
0
41309
TrEMBL
Secretory Pathway (Reliability: 1)
A0A6B9LUL6_9BASI
393
0
41392
TrEMBL
Secretory Pathway (Reliability: 1)
A0A653HGF7_9APIC
360
0
40977
TrEMBL
other Location (Reliability: 3)
A0A6B9LT41_9BASI
393
0
41544
TrEMBL
Secretory Pathway (Reliability: 1)
A0A0B7FIA8_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
605
1
65611
TrEMBL
other Location (Reliability: 4)
D2RJC7_ACIFV
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
443
0
49160
TrEMBL
-
M5BSC6_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
331
0
35038
TrEMBL
other Location (Reliability: 2)
A0A1D8NPX6_YARLL
580
0
64610
TrEMBL
other Location (Reliability: 1)
A0A0B7FT35_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
582
0
63743
TrEMBL
other Location (Reliability: 4)
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
CPS1 accumulates at an aberrant late endosomal compartment in mutants of the COPIB subcomplex. CPS1 is not delivered to the vacuolar lumen in COPIb mutants. Only mutations in COPIb subunits have an effect upon CPS1 sorting, whereas in COPIf mutants CPS1 localizes to the vacuolar lumen
additional information
-
GFP-Cps1p accumulates in a prevacuolar compartment as well as on the limiting membrane of the vacuole in rps5WW1-3 cells with WW domain mutations. In rsp5L733S cells, GFP-Cps1p is detectable in the lumen of the vacuole, some is also found on the limiting membrane of the vacuole. In bsd2delta cells, GFP-Cps1p accumulates in the limiting membrane of the vacuole. In tul1delta cells sorting is also impaired. No defect in GFP-Cps1p vacuolar sorting in sna3delta cells
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GFP-Cps1p expressed in rsp5delta cells expressing wild-type or mutant HA-Rsp5p with WW domain mutations W257A, W359A, and W415A. GFP-Cps1p expressed in rsp5delta cells expressing HARsp5pWT, HA-rsp5pL733S or HA-rsp5G555D. GFP-Cps1p expressed in tul1delta cells, bsd2delta cells and sna3delta cells
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
sorting of Cps1p into the luminal vesicles of multivesicular bodies requires ubiquitination of their cytosolic domains by the ubiquitin ligases Rsp5p and/or Tul1p, whereas Sna3p, another integral membrane protein, does not require ubiquitination for entry into multivesicular bodies. Sna3p follows an ubiquitination-independent, but Rsp5p-mediated, sorting pathway to the vacuole
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chikuma, T.; Kishii, M.; Taguchi, K.; Yajima, R.; Kato, T.; Loh, Y.P.; Ishii, Y.; Tanaka, A.
High-performance liquid chromatographic-colorimetric assay for glycine carboxypeptidase activity
J. Chromatogr. B
703
45-51
1997
Bos taurus, Mus musculus
brenda
Felix, F.; Brouillet, N.
Purification et proprietes de deux peptidases de levure de brasserie
Biochim. Biophys. Acta
122
127-144
1966
Saccharomyces cerevisiae
brenda
Gabriely, G.; Kama, R.; Gerst, J.E.
Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast
Mol. Cell. Biol.
27
526-540
2007
Saccharomyces cerevisiae
brenda
Watson, H.; Bonifacino, J.S.
Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p
Mol. Biol. Cell
18
1781-1789
2007
Saccharomyces cerevisiae
brenda
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