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Information on EC 3.4.17.4 - Gly-Xaa carboxypeptidase for references in articles please use BRENDA:EC3.4.17.4Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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Release of a C-terminal amino acid from a peptide in which glycine is the penultimate amino acid, e.g. Z-Gly-/-Leu
from yeast. In peptidase family M20, glutamate carboxypeptidase family
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hydrolysis of peptide bond
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exopeptidase, C-terminus, amino acid
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carboxypeptidase a
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EC 3.4.12.8
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formerly
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EC 3.4.17.9
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formerly
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EC 3.4.2.3
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formerly
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GLY-X carboxypeptidase
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glycine carboxypeptidase
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carboxypeptidase S
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4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe
benzoyl-Gly-Gly + H2O
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7.2% of the activity compared to benzyloxycarbonyl-Gly-Leu
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?
benzoyl-Gly-Lys + H2O
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2% of the activity compared to benzyloxycarbonyl-Gly-Leu
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benzyloxycarbonyl-Ala-Arg(NO2) + H2O
benzyloxycarbonyl-Ala + nitroarginine
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21% of the activity compared to benzyloxycarbonyl-Gly-Leu
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Benzyloxycarbonyl-Ala-Phe + H2O
Benzyloxycarbonyl-Ala + Phe
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10% of the activity compared to benzyloxycarbonyl-Gly-Leu
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Benzyloxycarbonyl-Glu-Tyr + H2O
Benzyloxycarbonyl-Glu + Tyr
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1.1% of the activity compared to benzyloxycarbonyl-Gly-Leu
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benzyloxycarbonyl-Gly-Glu + H2O
benzyloxycarbonyl-Gly + Glu
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31% of the activity compared to benzyloxycarbonyl-Gly-Leu
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benzyloxycarbonyl-Gly-Gly + H2O
benzyloxycarbonyl-Gly + Gly
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13% of the activity compared to benzyloxycarbonyl-Gly-Leu
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Benzyloxycarbonyl-Gly-Leu + H2O
Benzyloxycarbonyl-Gly + Leu
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best substrate
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Benzyloxycarbonyl-Gly-Phe + H2O
Benzyloxycarbonyl-Gly + Phe
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31% of the activity compared to benzyloxycarbonyl-Gly-Leu
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benzyloxycarbonyl-Phe-Leu + H2O
benzyloxycarbonyl-Phe + Leu
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1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu
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benzyloxycarbonyl-Phe-Met + H2O
benzyloxycarbonyl-Phe + Met
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1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu
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benzyloxycarbonyl-Phe-Phe + H2O
benzyloxycarbonyl-Phe + Phe
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1.5% of the activity compared to benzyloxycarbonyl-Gly-Leu
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dibenzyloxycarbonyl-Lys-Leu + H2O
dibenzyloxycarbonyl-Lys + Leu
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5.2% of the activity compared to benzyloxycarbonyl-Gly-Leu
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Gly-Leu-Gly + H2O
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3% of the activity compared to benzyloxycarbonyl-Gly-Leu
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Gly-Leu-Tyr + H2O
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12% of the activity compared to benzyloxycarbonyl-Gly-Leu
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additional information
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no hydrolysis of benzyloxycarbonyl-Leu-Leu, benzyloxycarbonyl-Leu-Phe, dibenzyloxycarbonyl-Lys-Phe, benzyloxycarbonyl-Phe-Ser, benzyloxycarbonyl-His-Leu, benzyloxycarbonyl-Pro-Try, benzyloxycarbonyl-Gly-Gly-Leu, benzyloxycarbonyl-Gly-Gly-Phe, benzyloxycarbonyl-Gly-Leu-NH2, benzyloxycarbonyl-Gly-Phe-NH2, benzyloxycarbonyl-Ala-Phe-NH2, benzyloxycarbonyl-Phe-Leu-NH2, benzyloxycarbonyl-Phe-Leu-NH2 and Gly-Leu
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4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe
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enzyme assay is based on the detection of the product using HPLC to separate it from the substrate
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4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe
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enzyme assay is based on the detection of the product using HPLC to separate it from the substrate
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CaCl2
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0.1 mM and 1 mM, 108.9% and 105.5% compared to a control without additives
Cd2+
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can reverse the inhibition by chelating agents
Co2+
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can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 62%, inhibits the enzyme at higher concentrations, 10 mM
MgCl2
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0.1 mM and 1 mM, 109.8% and 103.4% compared to a control without additives
Mn2+
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MnCl2, 0.1 mM and 1 mM, 106.3% and 104.2% compared to a control without additives
Mn2+
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can reverse the inhibition by chelating agents
Zn2+
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ZnSO4, 0.1 mM and 1 mM, 97.6% and 69.6% compared to a control without additives
Zn2+
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can reverse the inhibition by chelating agents at a concentration of 0.1 mM, reactivation of 88%, inhibits the enzyme at higher concentrations, 10 mM
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CuSO4
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complete inhibition at 1 mM, 39% inhibition at 0.1 mM
diisopropylphosphorofluoridate
iodoacetamide
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90% inhibition at a concentration of 5 mM
p-chloromercuribenzoate
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irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol
p-Chloromercuriphenylsulfonic acid
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0.1 mM and 1 mM, complete inhibition
PMSF
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0.1 mM and 1 mM, 78.1% and 21.4% compared to a control without additives
1,10-phenanthroline
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0.1 mM and 1 mM, 95.6% and 74.7% compared to a control without additives
1,10-phenanthroline
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Ki: 0.035 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+
Ag+
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AgNO3, 0.1 mM and 1 mM, complete inhibition
Ag+
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complete inhibition at a concentration of 1 mM
diisopropylphosphorofluoridate
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0.1 mM and 1 mM, 32.3% and 24.3% compared to a control without additives
diisopropylphosphorofluoridate
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no inhibition
EDTA
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0.1 mM and 1 mM, 105% and 96.1% compared to a control without additives
EDTA
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Ki: 0.013 mM, reversible by addtion of bivalent metal ions like Zn2+, Co2+, Cd2+ and Mn2+, Zn2+ and Co2+, at a concentration of 0.1 mM, are most effecient with 88% and 62% reactivation
iodoacetate
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0.1 mM and 1 mM, 110.5% and 94.8% compared to a control without additives
iodoacetate
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40% inhibition at a concentration of 5 mM, irreversible inhibition, no reactivation by addition of bivalent metal ions or SH-protecting reagents like cysteine and beta-mercaptomethanol
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additional information
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no effect by addition of FAD, FMN, NADPH, NADP+, NADH, NAD+
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0.0211
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly
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0.000000113
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six-week-old animal
0.000000115
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six-week-old animal
0.000000116
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six-week-old animal
0.000001917
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six-week-old animal
0.00000415
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six-week-old animal
0.00000845
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six-week-old animal
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4.8 - 5.4
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4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly as substrate, 50 mM sodium acetate buffer
6 - 6.2
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benzyloxycarbonyl-Gly-Leu as substrate, in presence of 0.5 M NaCl
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4.2 - 6.5
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pH 4.2: 28% of the maximal activity, pH 6.5: 43% of the maximal activity
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integral membrane protein, destined for the vacuolar lumen
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sorting of integral membrane protein Cps1p into the luminal vesicles of multivesicular bodies
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GFP-Cps1p expressed in rsp5delta cells expressing wild-type or mutant HA-Rsp5p with WW domain mutations W257A, W359A, and W415A. GFP-Cps1p expressed in rsp5delta cells expressing HARsp5pWT, HA-rsp5pL733S or HA-rsp5G555D. GFP-Cps1p expressed in tul1delta cells, bsd2delta cells and sna3delta cells
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plasmid pGFP-CPS1, which expresses GFP-CPS1
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additional information
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CPS1 accumulates at an aberrant late endosomal compartment in mutants of the COPIB subcomplex. CPS1 is not delivered to the vacuolar lumen in COPIb mutants. Only mutations in COPIb subunits have an effect upon CPS1 sorting, whereas in COPIf mutants CPS1 localizes to the vacuolar lumen
additional information
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GFP-Cps1p accumulates in a prevacuolar compartment as well as on the limiting membrane of the vacuole in rps5WW1-3 cells with WW domain mutations. In rsp5L733S cells, GFP-Cps1p is detectable in the lumen of the vacuole, some is also found on the limiting membrane of the vacuole. In bsd2delta cells, GFP-Cps1p accumulates in the limiting membrane of the vacuole. In tul1delta cells sorting is also impaired. No defect in GFP-Cps1p vacuolar sorting in sna3delta cells
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additional information
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sorting of Cps1p into the luminal vesicles of multivesicular bodies requires ubiquitination of their cytosolic domains by the ubiquitin ligases Rsp5p and/or Tul1p, whereas Sna3p, another integral membrane protein, does not require ubiquitination for entry into multivesicular bodies. Sna3p follows an ubiquitination-independent, but Rsp5p-mediated, sorting pathway to the vacuole
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CBPS_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
576
64597
Swiss-Prot
W0ZDY3_9MICO
451
48342
TrEMBL
A4YK08_BRASO
Bradyrhizobium sp. (strain ORS 278)
470
50188
TrEMBL
K0KHC4_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
579
65588
TrEMBL
A0A0H3C6J6_CAUVN
Caulobacter vibrioides (strain NA1000 / CB15N)
492
51456
TrEMBL
B8MMJ9_TALSN
Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006)
553
61333
TrEMBL
A0A0X8R3J9_9SPHN
491
51719
TrEMBL
H6RL74_BLASD
Blastococcus saxobsidens (strain DD2)
477
50798
TrEMBL
A0A2P2C7T6_9ZZZZ
453
48089
TrEMBL
M5BQN3_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
443
48753
TrEMBL
A2QF94_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
578
63641
TrEMBL
M5BJ38_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
545
59303
TrEMBL
A3RWM6_RALSL
510
54260
TrEMBL
G2SHZ7_RHOMR
495
53447
TrEMBL
A0A097NUZ4_VITVI
357
39081
TrEMBL
A0A0F4YI06_TALEM
546
60037
TrEMBL
A0A0M3R844_9ROSI
499
52927
TrEMBL
A1ZQY0_9BACT
486
53993
TrEMBL
A0A1J1GU63_PLAGA
385
44011
TrEMBL
U5HT00_MUCCI
394
41351
TrEMBL
A0A0B7FT35_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
582
63743
TrEMBL
K0KVP2_WICCF
Wickerhamomyces ciferrii (strain F-60-10 / ATCC 14091 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031)
571
63917
TrEMBL
F4GKR1_SPHCD
Sphaerochaeta coccoides (strain ATCC BAA-1237 / DSM 17374 / SPN1)
489
54484
TrEMBL
H0TSI8_9BRAD
493
52895
TrEMBL
D2RJC7_ACIFV
Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / VR4)
443
49160
TrEMBL
D7GRB1_9FIRM
450
49547
TrEMBL
H0T4G4_9BRAD
470
50047
TrEMBL
Q6NCQ7_RHOPA
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
493
53821
TrEMBL
W1Q877_OGAPD
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)
552
62060
TrEMBL
M5BSC6_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
331
35038
TrEMBL
F8EGG2_RUNSL
Runella slithyformis (strain ATCC 29530 / DSM 19594 / LMG 11500 / NCIMB 11436 / LSU 4)
485
53819
TrEMBL
H0SN98_BRAS3
Bradyrhizobium sp. (strain ORS 375)
470
49974
TrEMBL
B7Q1Z4_IXOSC
509
56312
TrEMBL
A0A099L7F1_9GAMM
494
54849
TrEMBL
A0A0B7FKE4_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
610
66142
TrEMBL
A0A0B7FIA8_THACB
Thanatephorus cucumeris (strain AG1-IB / isolate 7/3/14)
605
65611
TrEMBL
Q8IKI0_PLAF7
385
44254
TrEMBL
A0A060S072_PLARE
385
44138
TrEMBL
A3GGI9_PICST
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)
582
65092
TrEMBL
PYR1_HUMAN
2225
242984
Swiss-Prot
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Chikuma, T.; Kishii, M.; Taguchi, K.; Yajima, R.; Kato, T.; Loh, Y.P.; Ishii, Y.; Tanaka, A.
High-performance liquid chromatographic-colorimetric assay for glycine carboxypeptidase activity
J. Chromatogr. B
703
45-51
1997
Bos taurus, Mus musculus
brenda
Felix, F.; Brouillet, N.
Purification et proprietes de deux peptidases de levure de brasserie
Biochim. Biophys. Acta
122
127-144
1966
Saccharomyces cerevisiae
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Gabriely, G.; Kama, R.; Gerst, J.E.
Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast
Mol. Cell. Biol.
27
526-540
2007
Saccharomyces cerevisiae
brenda
Watson, H.; Bonifacino, J.S.
Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p
Mol. Biol. Cell
18
1781-1789
2007
Saccharomyces cerevisiae
brenda
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