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Information on EC 3.4.17.20 - Carboxypeptidase U and Organism(s) Bos taurus and UniProt Accession Q2KIG3

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.20 Carboxypeptidase U
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This record set is specific for:
Bos taurus
UNIPROT: Q2KIG3 not found.
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
tafia, thrombin-activatable fibrinolysis inhibitor, thrombin activatable fibrinolysis inhibitor, procpu, procarboxypeptidase b, carboxypeptidase u, thrombin-activable fibrinolysis inhibitor, procarboxypeptidase u, carboxypeptidase b2, plasma carboxypeptidase b, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
thrombin-activatable fibrinolysis inhibitor
-
Arginine carboxypeptidase
-
-
-
-
Carboxypeptidase B, pro-
-
-
-
-
Carboxypeptidase R
-
-
-
-
Carboxypeptidase-U
-
-
-
-
CPU
-
-
-
-
Plasma carboxypeptidase B
-
-
-
-
pro-pCPB
-
-
-
-
Procarboxypeptidase B
-
-
-
-
pTAFI
-
-
-
-
rTAFI
-
-
-
-
TAFI
-
-
-
-
TAFIa
-
-
-
-
Thrombin-activable fibrinolysis inhibitor
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
156621-18-0
-
37329-68-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hippuryl-Arg + H2O
hippuric acid + Arg
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tick carboxypeptidase inhibitor
proteinaceous inhibitor TCI which is found in in the hematophagous ixodid tick Rhipicephalus bursa consists of tandem structurally similar small modules, an N-terminal and a C-terminal domain. TCI inhibits TFAIa mainly through its C-terminal domain. The C-terminal residue His75 is cleaved and the new C-terminus Leu74 approaches and contacts the TAFIa catalytic zinc ion through its carboxylate atoms. In addition to this contact, the C-terminal domain of TCI itneracts with TFAIa through 15 hydrogen bonds and 5 hydrophobic contacts
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.44
hippuryl-Arg
Vmax: 46.05 micromol/l/min
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.74
hippuryl-Arg
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CBPB2_BOVIN
423
0
48822
Swiss-Prot
Secretory Pathway (Reliability: 2)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using the sitting-drop vapor diffusion method. TAFIa conforms to the alpha/beta-hydrolase fold of metallocarboxypeptidases and displays two unique flexible loops on the molecular surface, accounting for structural instability and susceptibility to proteolysis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified from plasma using a ECH-lysine sepharose column and anion-exchange chromatography
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sanglas, L.; Valnickova, Z.; Arolas, J.L.; Pallares, I.; Guevara, T.; Sola, M.; Kristensen, T.; Enghild, J.J.; Aviles, F.X.; Gomis-Rueth, F.X.
Structure of activated thrombin-activatable fibrinolysis inhibitor, a molecular link between coagulation and fibrinolysis
Mol. Cell
31
598-606
2008
Homo sapiens, Bos taurus (Q2KIG3), Bos taurus
Manually annotated by BRENDA team