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EC Tree
The enzyme appears in viruses and cellular organisms
Reaction Schemes
cleavage of the bond: (Ac)2-L-lysyl-D-alanyl-/-D-alanine
Synonyms
dd-carboxypeptidase, pbp4a, d-alanyl-d-alanine carboxypeptidase, penicillin binding protein 4, dd-carboxypeptidase-transpeptidase, pbp4b, d-alanyl-d-alanine-cleaving carboxypeptidase, penicillin-binding protein 4a,
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D-alanyl-D-alanine carboxypeptidase
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D-Alanyl-D-alanine hydrolase
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D-Alanyl-D-alanine-cleaving carboxypeptidase
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DD-Carboxypeptidase-transpeptidase
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penicillin binding protein 4
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penicillin-binding protein 4a
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zinc D-Ala-D-Ala carboxypeptidase
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additional information
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the enzyme belongs to the serine protease family
DD-Carboxypeptidase
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cleavage of the bond: (Ac)2-L-lysyl-D-alanyl-/-D-alanine
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hydrolysis of peptide bond
hydrolysis of peptide bond
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hydrolysis of peptide bond
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Acetyl2-L-Lys-D-Ala-D-Ala + H2O
Acetyl2-L-Lys-D-Ala + D-Ala
Nalpha,Nepsilon-diacetyl-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-Lys-D-Ala + D-Ala
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-
-
?
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Acetyl-L-Lys-D-Ala + D-Ala
peptidoglycan + H2O
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hydrolyzes peptidoglycan interpeptide bonds between a D-alanine and another D-amino acid residue in alpha-position to a free carboxylate
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additional information
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Acetyl2-L-Lys-D-Ala-D-Ala + H2O
Acetyl2-L-Lys-D-Ala + D-Ala
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?
Acetyl2-L-Lys-D-Ala-D-Ala + H2O
Acetyl2-L-Lys-D-Ala + D-Ala
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-
?
Acetyl2-L-Lys-D-Ala-D-Ala + H2O
Acetyl2-L-Lys-D-Ala + D-Ala
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-
?
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Acetyl-L-Lys-D-Ala + D-Ala
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-
?
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Acetyl-L-Lys-D-Ala + D-Ala
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additional information
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DAP contributes to resistance against NO required for the intracellular growth of the bacterium
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additional information
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the DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli
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additional information
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PBP4B shows penicillin-binding capacity and a low DD-carboxypeptidase activity
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additional information
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mechanism
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additional information
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weak beta-lactamase activity hydrolyzing penicillin into penicilloate
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additional information
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involved in bacterial cell wall metabolism
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additional information
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additional information
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DAP contributes to resistance against NO required for the intracellular growth of the bacterium
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?
additional information
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the DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli
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additional information
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involved in bacterial cell wall metabolism
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?
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Mg2+
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2-5 mM, optimal activity
Zinc
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a zinc enzyme
Zinc
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1 Zn2+ bound per enzyme molecule
Zinc
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Zn2+ cofactor is required for both DD-carboxypeptidase activity and binding of benzyl-penicillin
Zinc
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the Zn2+ ion is ligated by three histidine residues and located in a cleft in the C-terminal domain
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6-beta-iodopenicillinate
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causes permanent inactivation
L-3-mercaptoisobutyrate
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Infections
Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction.
Osteomyelitis
Identification of Penicillin Binding Protein 4 (PBP4) as a critical factor for Staphylococcus aureus bone invasion during osteomyelitis in mice.
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0.000001
3-Mercaptopropionate
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0.000001
L-3-mercaptoisobutyrate
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additional information
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8.03
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sequence calculation
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brenda
strain 544, ATCC 23448, a smooth virulent biovar 1 strain, gene dacF
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brenda
G
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brenda
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brenda
R39 and R61
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brenda
gene pbp4B
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brenda
the enzyme contains the mutation D261Y, relative to the SwissProt entry P24228; gene dacB
SwissProt
brenda
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brenda
additional information
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intracellular replication of Brucella abortus within murine macrophages and HeLa cells
brenda
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brenda
the enzyme contains a 5' leader sequence targeting it to the periplasm
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brenda
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brenda
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brenda
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brenda
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CBPM_STRAL
255
0
26190
Swiss-Prot
-
A0A2C9D6I1_9HYPH
276
0
29906
TrEMBL
-
J7T5U1_STRSL
265
1
29337
TrEMBL
-
A0A1C5XAN9_9FIRM
uncultured Coprococcus sp
128
0
14018
TrEMBL
-
A0A1F2Q5X0_RHOER
258
0
28679
TrEMBL
-
A0A7X6A026_9ACTN
248
1
26027
TrEMBL
-
A0A7W7QFV9_9PSEU
246
1
26796
TrEMBL
-
A0A2R7ZYP3_CARDV
251
0
28372
TrEMBL
-
A0A2P9F7K8_9ACTN
136
1
14446
TrEMBL
-
A0A841S3W5_9ACTN
253
1
26532
TrEMBL
-
A0A6C0PXB2_9ACTN
256
1
26031
TrEMBL
-
A0A2S6X7F4_9ACTN
244
1
25667
TrEMBL
-
A0A3P8K5X6_TSUPA
239
0
26145
TrEMBL
-
A0A3N6G550_9ACTN
245
1
25224
TrEMBL
-
A0A7Z0BF79_9ACTN
257
1
27549
TrEMBL
-
A0A177HLU8_9ACTN
245
1
25739
TrEMBL
-
A0A7Y9ZL01_9ACTN
253
1
27231
TrEMBL
-
A0A3N6J7W1_9ACTN
232
0
24058
TrEMBL
-
A0A1Y5TYI3_9RHOB
224
1
24073
TrEMBL
-
A0A7W7VI98_9PSEU
250
0
25738
TrEMBL
-
A0A3N6J1S2_9ACTN
244
1
25590
TrEMBL
-
A0A1C5PU59_9CLOT
uncultured Clostridium sp
420
0
47274
TrEMBL
-
A0A333V3M6_ACIBA
276
0
30950
TrEMBL
-
D3Q7X2_STANL
Stackebrandtia nassauensis (strain DSM 44728 / CIP 108903 / NRRL B-16338 / NBRC 102104 / LLR-40K-21)
263
1
28146
TrEMBL
-
A0A150HNB3_9GAMM
276
0
30867
TrEMBL
-
A0A7U9SWU8_9FIRM
198
0
22318
TrEMBL
-
A0A852Z8Q9_9ACTN
249
1
26102
TrEMBL
-
A0A2P8A6L9_9ACTN
244
1
25637
TrEMBL
-
A0A173U1R0_9FIRM
419
0
46990
TrEMBL
-
A0A7W7LYV7_9ACTN
230
0
23788
TrEMBL
-
A0A1S8MDX2_9CLOT
139
0
14948
TrEMBL
-
A0A1V5MHR6_9FIRM
365
1
39181
TrEMBL
-
A0A399H5R4_9ACTN
265
1
27789
TrEMBL
-
A0A2X0R221_9LACT
248
1
27478
TrEMBL
-
A0A7W9HGB8_9PSEU
195
0
20670
TrEMBL
-
A0A173SX12_9FIRM
870
0
94997
TrEMBL
-
A0A7U9RYK6_9FIRM
422
0
47574
TrEMBL
-
A0A7U9SKZ3_9FIRM
421
0
46854
TrEMBL
-
A0A1C5T129_9FIRM
uncultured Blautia sp
424
0
47703
TrEMBL
-
Q1J574_STRPF
Streptococcus pyogenes serotype M4 (strain MGAS10750)
247
1
27583
TrEMBL
-
A0A1K2FTY6_9ACTN
259
1
27055
TrEMBL
-
A0A140L2U9_9CLOT
485
1
51643
TrEMBL
-
A0A7W9JAT3_9ACTN
255
1
26754
TrEMBL
-
A0A7X5UV14_9PSEU
245
1
25690
TrEMBL
-
A0A399H523_9ACTN
293
1
30100
TrEMBL
-
D6B7Y2_9ACTN
255
0
26190
TrEMBL
-
A0A174G6S9_CLOSY
427
0
47368
TrEMBL
-
A0A376K099_ECOLX
117
0
13184
TrEMBL
-
Q3F005_BACTI
99
0
11519
TrEMBL
-
A0A378T473_MORLA
145
0
15868
TrEMBL
-
A0A1C5UMB1_9FIRM
uncultured Blautia sp
424
0
47595
TrEMBL
-
A0A6N2UZU2_9FIRM
uncultured Blautia sp
422
0
47922
TrEMBL
-
A0A517YAJ3_9BACT
245
0
25934
TrEMBL
-
A0A1M9IKQ3_9MYCO
237
0
25313
TrEMBL
-
A0A1U1AHA4_9MYCO
209
1
22568
TrEMBL
-
A0A3N6HS19_9ACTN
260
1
26420
TrEMBL
-
A0A174ZTK7_9FIRM
430
0
48779
TrEMBL
-
A0A174L0K6_9CLOT
145
0
16017
TrEMBL
-
A0A238L763_9RHOB
173
2
18441
TrEMBL
-
A0A1C5Q8G6_9FIRM
uncultured Faecalibacterium sp
866
0
94732
TrEMBL
-
D2PVN6_KRIFD
Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399)
255
1
26551
TrEMBL
-
A0A7Z7N7B5_9MYCO
282
0
30301
TrEMBL
-
Q3EKJ0_BACTI
286
1
32938
TrEMBL
-
A0A378Q0S4_MORBO
180
0
19813
TrEMBL
-
A0A173V6T9_9FIRM
420
0
47373
TrEMBL
-
A0A1E3H4E0_9HYPH
264
0
28381
TrEMBL
-
A0A3N6G4I6_9ACTN
135
1
14241
TrEMBL
-
A0A484IF95_9ARCH
182
0
19794
TrEMBL
-
A0A221W9J0_9PSEU
243
0
26212
TrEMBL
-
N0CVM8_STRMI
269
1
27449
TrEMBL
-
A0A174L7Q4_9FIRM
132
0
14516
TrEMBL
-
A0A1C5R9M1_9FIRM
uncultured Roseburia sp
129
0
14421
TrEMBL
-
A0A7U9DUR2_STRLI
244
1
25637
TrEMBL
-
A0A6N3G0H5_9FIRM
433
0
49069
TrEMBL
-
A0A1V5TYW4_9FIRM
387
0
41978
TrEMBL
-
A0A0M2HFE0_9MICO
284
0
29857
TrEMBL
-
A0A100JHP1_STRSC
259
0
27533
TrEMBL
-
A0A239SWC0_9BURK
271
0
29128
TrEMBL
-
Q3F069_BACTI
243
0
27842
TrEMBL
-
G0PQY8_9ACTN
260
1
26425
TrEMBL
-
A0A1F2PL50_9FIRM
181
0
19239
TrEMBL
-
A0A1D2IER7_9ACTN
244
1
25732
TrEMBL
-
A0A174VI29_PHOVU
132
0
15118
TrEMBL
-
A0A6M2YSH4_9CAUD
131
0
14735
TrEMBL
other Location (Reliability: 2)
A0A5J4DST0_9BACT
598
0
65672
TrEMBL
-
A0A180F629_9BACT
132
0
14647
TrEMBL
-
A0A3N6HY77_9ACTN
130
0
13371
TrEMBL
-
A0A231I4C1_BACTU
243
0
27630
TrEMBL
-
A0A0I9RLN4_BACFN
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow)
131
0
14822
TrEMBL
-
A0A0M7B6L7_9RHOB
224
2
24450
TrEMBL
-
A0A7W8FCN7_9ACTN
230
0
23879
TrEMBL
-
A0A238KSH3_9RHOB
224
2
24461
TrEMBL
-
A0A1C5NY12_9FIRM
uncultured Faecalibacterium sp
137
0
15050
TrEMBL
-
A0A2X2M082_STRGR
260
1
26425
TrEMBL
-
A0A1V5FRD9_9BACT
501
0
52367
TrEMBL
-
K4QW03_STRDJ
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
244
1
25722
TrEMBL
-
A0A6L5C1G8_9PSED
263
0
27979
TrEMBL
-
A0A378YNZ7_9BURK
271
0
29027
TrEMBL
-
A0A6B3Q2R4_ENTFC
220
1
24868
TrEMBL
-
A0A4P7CDJ3_9PSEU
246
1
26200
TrEMBL
-
A0A162GGR5_9MICO
351
1
35250
TrEMBL
-
G0Q916_9ACTN
232
0
24086
TrEMBL
-
A0A8B4DV38_9MYCO
282
0
30367
TrEMBL
-
A0A1Y5TZ66_9RHOB
224
2
24436
TrEMBL
-
W5SU38_BORAN
134
0
15939
TrEMBL
-
A9WQ35_RENSM
Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
166
0
18217
TrEMBL
-
A0A5P9PSN8_9PSEU
252
0
26007
TrEMBL
-
A0A485HDT0_PSEAI
268
0
29551
TrEMBL
-
A0A378UUW3_MYCFO
78
0
8420
TrEMBL
-
A0A7Z0DQG3_9ACTN
254
1
27044
TrEMBL
-
A0A6N2Y5A7_EUBLI
101
0
11270
TrEMBL
-
A0A654M8C6_9ARCH
283
0
30519
TrEMBL
-
A0A1Y5TWB6_9RHOB
224
2
24372
TrEMBL
-
A0A839XPC5_9PSEU
197
0
21019
TrEMBL
-
A0A3N6EEY4_9ACTN
255
0
26189
TrEMBL
-
A9WQ34_RENSM
Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)
52
0
5935
TrEMBL
-
A0A0E1BY47_BORHE
264
0
31124
TrEMBL
-
A0A1C6GUD4_9FIRM
uncultured Flavonifractor sp
415
0
46701
TrEMBL
-
A0A3B0MUV1_9RHOB
224
2
24560
TrEMBL
-
A0A3B0MXZ6_9RHOB
224
2
24578
TrEMBL
-
A0A1R7MJG3_CLODI
197
0
21924
TrEMBL
-
B0NEY6_CLOS5
Clostridium scindens (strain ATCC 35704 / DSM 5676 / VPI 13733 / 19)
421
0
46805
TrEMBL
-
A0A2P9F356_9ACTN
143
1
15427
TrEMBL
-
K0K725_SACES
Saccharothrix espanaensis (strain ATCC 51144 / DSM 44229 / JCM 9112 / NBRC 15066 / NRRL 15764)
241
1
25144
TrEMBL
-
W6TI52_9SPIR
127
1
14900
TrEMBL
-
A0A3N6GPC0_9ACTN
259
0
26743
TrEMBL
-
A0A839XH41_9PSEU
247
1
25887
TrEMBL
-
A0A1C5XLR6_9CLOT
uncultured Clostridium sp
420
0
47270
TrEMBL
-
A0A1C5KV16_9CLOT
uncultured Clostridium sp
426
0
48263
TrEMBL
-
A0A1C6L394_9CLOT
uncultured Clostridium sp
145
0
16017
TrEMBL
-
A0A1C5XGA6_9FIRM
uncultured Flavonifractor sp
415
0
46673
TrEMBL
-
A0ACE5_STRA7
Streptomyces ambofaciens (strain ATCC 23877 / 3486 / DSM 40053 / JCM 4204 / NBRC 12836 / NRRL B-2516)
244
1
25620
TrEMBL
-
A0A6N3DVP2_EUBLI
244
0
26351
TrEMBL
-
A0A173RCK3_9FIRM
330
0
36711
TrEMBL
-
A0A6N8HPL9_9BACI
356
0
39660
TrEMBL
-
A0A5P9Q5U7_9MICO
352
1
37294
TrEMBL
-
W5SNG7_9SPIR
275
1
32395
TrEMBL
-
A0A378TIR0_9MYCO
290
0
31021
TrEMBL
-
A0A7K0CJJ2_9ACTN
Streptomyces smaragdinus
245
1
25525
TrEMBL
-
A0A1V5NKG3_9FIRM
94
0
10097
TrEMBL
-
A0A1C6GD51_9CLOT
uncultured Clostridium sp
421
0
47327
TrEMBL
-
A0A379MNT7_9BACT
136
0
15462
TrEMBL
-
A0A0D0S2J0_9BACI
222
0
24610
TrEMBL
-
A0A3B0MZJ5_9RHOB
226
1
24845
TrEMBL
-
A0A6N3CPU1_EUBLI
200
1
21761
TrEMBL
-
A0A6C0QDT8_9ACTN
244
0
25659
TrEMBL
-
A0A2X2M0L3_STRGR
232
0
24083
TrEMBL
-
A0A173RHT7_9FIRM
265
0
29164
TrEMBL
-
A0A7U9RGX0_9FIRM
205
0
23024
TrEMBL
-
A0A173VSU1_9FIRM
419
0
47326
TrEMBL
-
Q3EVF4_BACTI
243
0
27688
TrEMBL
-
Q3EK49_BACTI
246
1
27963
TrEMBL
-
A0A1F2PC25_9FIRM
85
1
9051
TrEMBL
-
A0A4Q4DFT2_9ACTN
245
1
25485
TrEMBL
-
A0A378PXK3_MORBO
180
0
19841
TrEMBL
-
A0A7W7TWR4_9ACTN
Streptomyces nymphaeiformis
244
0
25657
TrEMBL
-
A0A2N7RST4_9BURK
145
0
14840
TrEMBL
-
A0A1C6AVQ8_9CLOT
uncultured Clostridium sp
261
0
28468
TrEMBL
-
A0A1C5PA27_9FIRM
uncultured Ruminococcus sp
426
0
47136
TrEMBL
-
A0A2K9EAV7_9FIRM
361
1
38258
TrEMBL
-
A0A173ZEU1_9FIRM
128
0
14018
TrEMBL
-
A0A1B9ER55_9ACTN
131
0
13807
TrEMBL
-
F4MYM5_YEREN
234
0
26355
TrEMBL
-
A0A6N2T5F5_9FIRM
421
0
47256
TrEMBL
-
A0A117EEA3_STRSC
362
1
38388
TrEMBL
-
A0A174KBK5_9FIRM
421
0
47140
TrEMBL
-
Q3EPD3_BACTI
247
1
28256
TrEMBL
-
A0A1V5FGS8_9PROT
274
0
29122
TrEMBL
-
A0A3N6GH77_9ACTN
127
1
13028
TrEMBL
-
A0A2N7QW40_9GAMM
423
0
45729
TrEMBL
-
A0A1C5YII4_9FIRM
uncultured Blautia sp
424
0
47674
TrEMBL
-
A0A173VW24_9FIRM
745
0
81042
TrEMBL
-
A0A399GLA9_9ACTN
140
1
14626
TrEMBL
-
F7LPI8_BACFG
131
0
14780
TrEMBL
-
K8E2Q5_CARML
257
0
29139
TrEMBL
-
A0A401MV60_9ACTN
99
0
10927
TrEMBL
-
A0A238KT64_9RHOB
224
2
24445
TrEMBL
-
A0A1V2RG48_9ACTN
244
1
25924
TrEMBL
-
A0A1V2RIX9_9ACTN
244
1
25984
TrEMBL
-
3.4.17.14 Zinc D-Ala-D-Ala carboxypeptidase
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The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).