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Information on EC 3.4.16.4 - serine-type D-Ala-D-Ala carboxypeptidase
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EC Tree
3.4.16.4 serine-type D-Ala-D-Ala carboxypeptidaseSpecify your search results
Word Map
- 3.4.16.4
- beta-lactams
- peptidoglycan
- staphylococcus
- aureus
- streptococcus
- pneumonia
-
methicillin-resistant
- cephalosporin
- gg
- methicillin
-
penicillin-resistant
- ampicillin
- bilirubin
-
sortase
-
gamma-glutamyl
- vancomycin
- cefotaxime
- oxacillin
-
transpeptidation
-
pneumococci
- faecium
- ceftriaxone
- carbapenems
- transglycosylase
- imipenem
-
penicillin-susceptible
- ceftazidime
- murein
-
methicillin-susceptible
- benzylpenicillin
- meropenem
- cefoxitin
- acyl-enzyme
- muropeptides
-
mic90s
- cefixime
-
cephems
- piperacillin
-
ampicillin-resistant
-
lpxtg
- cefuroxime
- penicillinase
- aztreonam
-
vancomycin-resistant
- cefaclor
-
beta-lactamase-negative
- synthesis
-
sccmec
-
beta-lactamase-producing
- nitrocefin
- sulbactam
- medicine
- analysis
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Preferential cleavage: (Ac)2-L-Lys-D-Ala-/-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
Synonyms
transpeptidase, penicillin-binding protein, pbp2x, pbp1a, pbp1b, penicillin binding proteins, pbp 2, pbp 3, pbp 5, dd-peptidase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
carboxypeptidase I
-
-
-
-
CPase
-
-
-
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D-alanine carboxypeptidase
D-alanine carboxypeptidase I
-
-
-
-
D-alanyl carboxypeptidase
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-
-
-
D-alanyl-D-alanine carboxypeptidase
D-Alanyl-D-alanine hydrolase
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-
-
-
D-alanyl-D-alanine peptidase
-
-
-
-
D-alanyl-D-alanine transpeptidase
D-alanyl-D-alanine-carboxypeptidase
D-alanyl-D-alanine-cleaving peptidase
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-
-
-
D-alanyl-D-alanine-cleaving-peptidase
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-
-
-
DacA-1
DacB
Dbv7
DD-Carboxypeptidase
DD-CPase
DD-CPase1
DD-peptidase
DD-transpeptidase
PBP
PBP 5
PBP 6
-
the DD-carboxypeptidase activity of PBP 6 is lower than that of PBP 5
PBP-5*
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-
-
-
PBP-6B
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-
-
-
PBP1b
PBP3
PBP4
PBP4a
PBP5
penicillin binding protein 5
penicillin-binding protein
penicillin-binding protein 5
penicillin-binding protein 5a
SCO4439
transpeptidase
VanX
VanY
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-
-
-
VanY(D) DD-carboxypeptidase
VanYD
VanYn
VC_0947
D-alanine carboxypeptidase
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-
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D-alanyl-D-alanine carboxypeptidase
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-
-
-
D-alanyl-D-alanine-carboxypeptidase
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-
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DD-Carboxypeptidase
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-
-
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DD-peptidase
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-
-
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DD-peptidase
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-
DD-transpeptidase
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-
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PBP 5
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the DD-carboxypeptidase activity of PBP 5 is higher than that of PBP 6
PBP5
-
-
-
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penicillin binding protein 5
-
-
-
-
VanX
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage: (Ac)2-L-Lys-D-Ala-/-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
Preferential cleavage: (Ac)2-L-Lys-D-Ala-/-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
VanY, carboxypeptidase activity exclusively
-
Preferential cleavage: (Ac)2-L-Lys-D-Ala-/-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
VanY, carboxypeptidase activity exclusively
-
-
Preferential cleavage: (Ac)2-L-Lys-D-Ala-/-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of peptide bond
transpeptidation
hydrolysis of peptide bond
-
-
exopeptidase, C-terminus, amino acid
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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CAS REGISTRY NUMBER
COMMENTARY
9077-67-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid + H2O
?
-
-
-
-
?
(R)-[2-(benzoylamino)propionylsulfanyl]acetic acid + H2O
?
-
-
-
?
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine + H2O
2-(N-glycyl-L-cysteinyl)acetyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-cysteaminyl)propanoyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate + H2O
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanine + D-thiolactate
-
very efficient substrate for R61 DD-peptidase
-
-
?
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanine + D-alanine
-
-
-
-
?
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanine + D-alanine
-
-
-
-
?
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O
(2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
(2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate
Ac2-L-Lys-D-Ala-Gly-L-Ala
?
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Gln
?
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Gln + Gly-L-Ala
Ac2-L-Lys-D-Ala-Gly-L-Ala + ?
-
transpeptidation
-
-
r
Ac2-L-Lys-D-Ala-Gly-L-Leu
?
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Leu + D-alanine
Ac2-L-Lys-D-Ala-D-Ala + ?
-
transpeptidation
-
-
r
acetyl-L-Ala-D-Glu-L-Lys(Ac)-D-Ala-D-Ala + H2O
acetyl-L-Ala-D-Glu-L-Lys(Ac)-D-Ala + D-Ala
-
-
-
-
?
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala-D-Ala + H2O
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala + D-Ala
-
-
-
-
?
Gly-gamma-L-Glu-D-Ala-D-Ala + H2O
Gly-gamma-L-Glu-D-Ala + D-Ala
-
-
-
?
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala + H2O
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala + D-Ala
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-alanine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-leucine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-leucine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-norleucine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-norleucine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + glycine
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-alanine + H2O
?
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-leucine + H2O
?
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-phenylalanine + H2O
?
-
-
-
-
?
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine + H2O
?
-
-
-
-
?
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine + H2O
?
-
-
-
-
?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O
?
-
only substrate of penicillin-binding protein 5, but not of penicillin-binding protein 6
-
-
?
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-Ala
-
-
-
?
N-acetyl-L-Lys-D-Ala-D-Ala + H2O
N-acetyl-L-Lys-D-Ala + D-Ala
-
-
-
-
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + D-alanine
-
-
-
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine + H2O
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanine + D-alanine
-
-
-
?
N-benzoyl-D-Ala + H2O
benzoate + D-Ala
-
substrate binding structure
-
-
?
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine + H2O
?
-
-
-
-
?
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
N,N'-diacetyl-L-Lys-D-Ala + D-Ala
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-lactate + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-lactate
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate + H2O
?
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
-
substrate in DD-carboxypeptidase activity assay
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
D-alanine + Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanine
Nalpha,Nepsilon-diacetyl-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-Lys-D-Ala + D-Ala
-
-
-
?
Nalpha,Neta-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
-
substrate for assay of DD-carboxypeptidase activity
-
-
?
Nalpha-(beta-1,4 acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)L-Lys-D-Ala-D-Ala + H2O
Nalpha-(beta-1,4 acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)L-Lys-D-Ala + D-Ala
-
-
-
-
?
Nalpha-(beta-1,4-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)-L-lysyl-D-alanyl-D-alanine + H2O
Nalpha-(beta-1,4-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)-L-lysyl-D-alanine + D-alanine
-
-
-
-
?
Nalpha-acetyl-L-Lys-D-Ala-D-Ala + H2O
?
substrate is used to detect the hydrolyzing activity of PBP4a
-
-
?
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Acetyl-L-Lys-D-Ala + D-Ala
-
-
?
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala + D-Ala
-
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
D-Ala + UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala