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Information on EC 3.4.16.2 - lysosomal Pro-Xaa carboxypeptidase and Organism(s) Homo sapiens and UniProt Accession P42785

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Homo sapiens
UNIPROT: P42785 not found.
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Cleavage of a -Pro-/-Xaa bond to release a C-terminal amino acid
Synonyms
carboxypeptidase, plasma carboxypeptidase, prolyl carboxypeptidase, lysosomal carboxypeptidase, carboxypeptidase a6, angiotensinase c, lysosomal pro-x carboxypeptidase, matrix pk activator, serine protease prolylcarboxypeptidase, proline carboxypeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
prolylcarboxypeptidase
-
aminoacylproline carboxypeptidase
-
-
-
-
angiotensinase C
-
-
-
-
carboxypeptidase A6
-
-
Carboxypeptidase P
-
-
-
-
carboxypeptidase, aminoacylproline
-
-
-
-
carboxypeptidase, peptidylprolylamino acid
-
-
-
-
CPA6
-
-
endothelial cell prekallikrein activator
-
-
HUVEC PK activator
-
-
lysosomal carboxypeptidase
-
-
-
-
lysosomal carboxypeptidase C
-
-
-
-
matrix PK activator
-
-
PCP
-
-
-
-
proline carboxypeptidase
-
-
-
-
proline-specific carboxypeptidase P
-
-
-
-
prolyl carboxypeptidase
-
-
-
-
prolyl-carboxypeptidase
-
-
prolylcarboxypeptidase
serine protease prolylcarboxypeptidase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-64-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-methoxycoumarin-4-acetyl-Pro-Pro-Lys + H2O
?
show the reaction diagram
-
-
-
?
3-(2-furyl)acryloyl-peptide + H2O
?
show the reaction diagram
-
substrate used in the carboxypeptidase assay
-
-
?
Ala-Pro-4-nitroanilide + H2O
Ala-Pro + 4-nitroaniline
show the reaction diagram
angiotensin II + H2O
?
show the reaction diagram
angiotensin III + H2O
?
show the reaction diagram
-
-
-
-
?
angiotensin III + H2O
angiotensin 2-7 + phenylalanine
show the reaction diagram
-
mutant DELTA317-496 is able to carry out this reaction
-
-
?
Gly-Pro-4-nitroanilide + H2O
Gly-Pro + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-benzyloxycarbonyl-L-Pro-L-Ala + H2O
N-benzyloxycarbonyl-L-Pro + L-Ala
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Pro-Ala + H2O
N-benzyloxycarbonyl-Pro + Ala
show the reaction diagram
N-benzyloxycarbonyl-Pro-Leu + H2O
N-benzyloxycarbonyl-Pro + Leu
show the reaction diagram
-
12% of the activity with angiotensin III
-
-
?
N-benzyloxycarbonyl-Pro-Phe + H2O
N-benzyloxycarbonyl-Pro + Phe
show the reaction diagram
N-benzyloxycarbonyl-Pro-Ser + H2O
N-benzyloxycarbonyl-Pro + Ser
show the reaction diagram
-
7% of the activity with angiotensin III
-
-
?
N-benzyloxycarbonyl-Pro-Val + H2O
N-benzyloxycarbonyl-Pro + Val
show the reaction diagram
prekallikrein + H2O
?
show the reaction diagram
-
-
-
?
saralasin + H2O
?
show the reaction diagram
-
-
-
-
?
YPRPIHPA + H2O
?
show the reaction diagram
-
using a peptidomic approach a single peptide YPRPIHPA is identified as a novel substrate for PRCP in human cerebrospinal fluid. The peptide YPRPIHPA is from the extracellular portion of human endothelin B receptor like protein 2
-
-
?
[des-Arg9]bradykinin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
YPRPIHPA + H2O
?
show the reaction diagram
-
using a peptidomic approach a single peptide YPRPIHPA is identified as a novel substrate for PRCP in human cerebrospinal fluid. The peptide YPRPIHPA is from the extracellular portion of human endothelin B receptor like protein 2
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
optimal activity at 0.01 mM
MgCl2
-
optimal activity at 1 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3R,4R)-4-[3-(4-chlorophenyl)-1H-pyrazol-5-yl]-1-(2,2-dimethylpropyl)-3-(4-fluorophenyl)piperidine
-
compound shows improved oral bioavailabilities
2-[4-[3-(4-fluorophenyl)-1-methyl-1H-pyrazol-5-yl]-1-(2-phenylethyl)piperidin-4-yl]pyridine
-
most potent compound in the series
angiotensin I
-
inhibits the mutant DELTA317-496 in a dose-dependent manner
angiotensin II
angiotensin III
-
inhibits the mutant DELTA317-496 in a dose-dependent manner
anti-PRCP
-
-
-
antipain
benzyloxycarbonyl-Pro-Pro-aldehyde dimethyl acetal
-
IC50: 0.001 mM
benzyloxycarbonyl-Pro-Pro-aldehyde-dimethyl acetal
-
-
benzyloxycarbonyl-Pro-prolinal
-
IC50: 0.5 mM, inhibits hydrolysis of H-Gly-Pro-4-nitroanilide
Benzylsuccinic acid
-
-
bradykinin
corn trypsin inhibitor
-
diisopropyl fluorophosphate
diisopropyl fluorophosphonate
ebelactone B
-
noncompetitive
HgCl2
-
IC50: 0.5 mM
leupeptin
N-(9-fluorenyl)methoxycarbonyl-Ala-Pyr-CN
-
IC50: 50 nM
N-(9-fluorenyl)methoxycarbonyl-aminoacylpyrrolidine-2-nitrile
-
IC50: 0.1 mM
N-benzyloxycarbonyl-Pro-prolinal
-
-
NEM
-
3 mM, 30% inhibition
p-chloromercuriphenylsulfonic acid
-
1 mM, 15% inhibition
PCMB
-
1 mM, 15% inhibition
pepstatin
phenylmethyl sulfonyl fluoride
-
3 mM
phenylmethylsulfonyl fluoride
potato carboxypeptidase inhibitor
-
-
-
protease inhibitors of the blood plasma
-
-
-
Z-Pro-prolinal
-
-
[(3S,4R)-1-tert-butyl-4-(2,4-difluorophenyl)pyrrolidin-3-yl](4-[(1Z)-1-[(2,5-dichlorophenyl)amino]-3-iminobut-1-en-1-yl]piperidin-1-yl)methanone
-
-
[(3S,4R)-1-tert-butyl-4-(2,4-difluorophenyl)pyrrolidin-3-yl](4-[(1Z)-1-[(3-chloro-4-fluorophenyl)amino]-3-iminoprop-1-en-1-yl]piperidin-1-yl)methanone
-
-
[4-[1-(3-chloro-4-fluorophenyl)-1H-pyrazol-5-yl]piperidin-1-yl][(1R,2R,4S)-2-(2,4-difluorophenyl)-4-(dimethylamino)-4-methylcyclopentyl]methanone
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
in cells, treated with 30 nM dexamethasone plus 1 nM DAMGO ([D-Ala2, N-Me-Phe4, Gly5-ol]-Enkephalin), the prolyl-carboxypeptidase gene is up-regulated
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.5 - 3.1
Ala-Pro-4-nitroanilide
1
angiotensin II
-
-
2
angiotensin III
-
-
4
Gly-Pro-4-nitroanilide
-
pH 5.5, 37┬░C
4.7
N-benzyloxycarbonyl-L-Pro-L-Ala
-
-
0.77
N-benzyloxycarbonyl-Pro-Phe
-
-
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