Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.15.6 - cyanophycinase and Organism(s) Pseudomonas anguilliseptica and UniProt Accession Q8KQN8

for references in articles please use BRENDA:EC3.4.15.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.15 Peptidyl-dipeptidases
                3.4.15.6 cyanophycinase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas anguilliseptica
UNIPROT: Q8KQN8 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Pseudomonas anguilliseptica
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
cyanophycinase, cgpase, cphb1, extracellular cgpase, cpheal, cphb2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyanophycin granule polypeptidase
-
extracellular CGPase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
the enzyme seems to belong to the familiy of serine proteases
PATHWAY SOURCE
PATHWAYS
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
131554-16-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
show the reaction diagram
-
-
-
?
[L-Asp(4-L-Arg)]n + H2O
[L-Asp(4-L-Arg)]n-1 + [L-Asp(4-L-Arg)]
show the reaction diagram
also called cyanophycin grana polypeptide (CPG), is a large copolymer of aspartate and arginine
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[L-Asp(4-Arg)]n + H2O
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
49% inhibition at 60 nM, 46% inhibition at 30 nM
leupeptin
13% inhibition within the range 0.001 nM to 0.01 nM
N-bromo-succinimide
100% inhibition at 5 nM, 99% inhibition at 1 nM
pefabloc
i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 92% inhibition at 80 nM, 13.6% inhibition at 8 nM
phenylmethylsulfonyl fluoride
63% inhibition at 10 nM, 28% inhibition at 1 nM
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.92
calculated from the sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
cells grown in medium containing cyanophycin granules
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CPHE_PSEAG
417
1
44767
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
monomer, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from culture supernatant of Pseudomonas anguilliseptica cells grown in medium containing cyanophycin granules, anion-exchange chromatography and L-arginine-agarose affinity chromatography. In case of heterologous expression in Escherichia coli the enzyme was not secreted and thus, isolation was done from the cell lysate
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli XL1-Blue
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
in-gel renaturation method
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Obst, M.; Oppermann-Sanio, F.B.; Luftmann, H.; Steinbchel, A.
Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI
J. Biol. Chem.
277
25096-25105
2002
Pseudomonas anguilliseptica (Q8KQN8), Pseudomonas anguilliseptica, Pseudomonas anguilliseptica BI (Q8KQN8)
Manually annotated by BRENDA team
Fueser, G.; Steinbuechel, A.
Analysis of genome sequences for genes of cyanophycin metabolism: identifying putative cyanophycin metabolizing prokaryotes
Macromol. Biosci.
7
278-296
2007
Acetivibrio thermocellus, Acinetobacter baylyi, Caldanaerobacter subterraneus subsp. tengcongensis (Q8R6L6), Caulobacter vibrioides, Caulobacter vibrioides CB15, Chloroflexus aurantiacus, Chloroflexus aurantiacus J-10-fl, Chromohalobacter salexigens, Chromohalobacter salexigens DSM 3043, Clostridium beijerinckii, Clostridium beijerinckii NCIMB 8052, Clostridium botulinum, Clostridium perfringens, Clostridium tetani, Clostridium tetani E88, Colwellia psychrerythraea, Crocosphaera watsonii, Crocosphaera watsonii WH 8501, Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Desulfitobacterium hafniense, Desulfitobacterium hafniense DCB-2, Francisella tularensis, Gemmata obscuriglobus, Gloeobacter violaceus, Janibacter sp., Mycobacterium marinum, Nostoc punctiforme, Nostoc sp., Polaromonas sp., Pseudomonas anguilliseptica (Q8KQN8), Pseudomonas anguilliseptica BI (Q8KQN8), Rubrivivax gelatinosus, Rubrivivax gelatinosus PM1, Synechococcus sp., Syntrophomonas wolfei, Syntrophomonas wolfei Goettingen, Thermoanaerobacter ethanolicus, Thermosynechococcus vestitus, Trichodesmium erythraeum, Trichormus variabilis (O86108)
Manually annotated by BRENDA team