Information on EC 3.4.15.4 - Peptidyl-dipeptidase B

for references in articles please use BRENDA:EC3.4.15.4
Word Map on EC 3.4.15.4
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Bos taurus

top print hide
EC NUMBER
COMMENTARY hide
3.4.15.4
-
top print hide
RECOMMENDED NAME
GeneOntology No.
Peptidyl-dipeptidase B
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of a C-terminal dipeptide or exceptionally a tripeptide
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
147014-93-5
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-Gly-Ala-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Ala + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
-
-
2-aminobenzoyl-Gly-Asn-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Asn + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
-
-
2-aminobenzoyl-Gly-Asp-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Asp + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
-
-
2-aminobenzoyl-Gly-Lys-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Lys + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
-
-
2-aminobenzoyl-Gly-Ser-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Ser + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
-
-
2-aminobenzoyl-Gly-Thr-(4-nitro)Phe-Arg + H2O
2-aminobenzoyl-Gly-Thr + 4-nitro-L-phenylalanyl-L-arginine
show the reaction diagram
-
-
-
-
-
Atriopeptin II + H2O
Atriopeptin I + ?
show the reaction diagram
Atriopeptin II peptide Ser103-Arg125 + H2O
Atriopeptin I peptide Ser103-Ser123 + Phe-Arg
show the reaction diagram
-
-
-
-
Atriopeptin III peptide Ser103-Tyr126 + H2O
Atriopeptin I + Phe-Arg-Tyr
show the reaction diagram
-
-
-
-
Benzoyl-Asn-Ser-Phe-Arg-Tyr + H2O
Benzoyl-Asn-Ser-Phe + Arg-Tyr
show the reaction diagram
-
-
successive dipeptidyl cleavage after longer incubation yielding benzoyl-Asn + Ser-Phe
-
Benzoyl-Gly-Asn-Ser + H2O
?
show the reaction diagram
-
-
-
-
-
benzoyl-Gly-His-Leu + H2O
?
show the reaction diagram
-
-
-
-
-
Benzoyl-Gly-Phe-Ser-Arg + H2O
?
show the reaction diagram
-
-
-
-
-
[Mercaptopropionyl105,(D)Ala107]-atriopeptinIII-NH2 peptide + H2O
Analog(1-18) + Phe-Arg-Tyr-NH2
show the reaction diagram
-
enzyme acts exclusively as tripeptidyl carboxyhydrolase
-
-
additional information
?
-
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
-
metallopeptidase
additional information
-
in contrast to peptidyl-dipeptidase A (EC 3.4.15.1) it displays no dependence on Cl-
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2-D-Methylsuccinyl-L-Pro
-
-
3-Mercaptopropanoyl-L-Pro
-
-
Benzoyl-Asn-Ser-Phe-Arg-Tyr-NH2
-
competitive inhibitor of 2-aminobenzoyl-Gly-Ala-Phe(4-nitro)-Arg hydrolysis and benzoyl-Asn-Ser-Phe-Arg-Tyr hydrolysis
Bradykinin potentiating factor
-
-
-
D-2-Methyl-3-mercaptopropanoyl-L-Pro
-
i.e. captopril
DTT
-
-
mercaptoethanol
-
-
additional information
-
marked substrate inhibition with benzoyl-Gly-His-Leu, but little or no substrate inhibition with benzoyl-Gly-His-Gly; the enzyme is 5500 times less sensitive to D-Cys-L-Pro, the very potent inhibitor of angiotensin I-converting enzyme (EC 3.4.15.1)
-
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.102
2-Aminobenzoyl-Gly-Ala-(4-nitro)Phe-Arg
-
-
0.09 - 0.213
2-Aminobenzoyl-Gly-Asp-(4-nitro)Phe-Arg
0.129
2-Aminobenzoyl-Gly-Lys-(4-nitro)Phe-Arg
-
-
0.14
2-Aminobenzoyl-Gly-Ser-(4-nitro)Phe-Arg
-
-
0.1
2-Aminobenzoyl-Gly-Thr-(4-nitro)Phe-Arg
0.124 - 0.27
Atriopeptin I
0.025
Atriopeptin II
-
-
1.13
benzoyl-Gly-Gly-Phe-Arg
-
-
2.4
benzoyl-Gly-His-Gly
-
-
0.8
benzoyl-Gly-His-Leu
-
-
0.12
benzoyl-Gly-Ser-Phe-Arg
-
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
-
-
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
6.5: about 65% of activity maximum, 9: about 60% of activity maximum
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000
-
bovine, sucrose density gradient sedimentation, gel filtration
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 3.4.15.4)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)