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Information on EC 3.4.15.1 - peptidyl-dipeptidase A and Organism(s) Rattus norvegicus and UniProt Accession P47820

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.15 Peptidyl-dipeptidases
                3.4.15.1 peptidyl-dipeptidase A
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This record set is specific for:
Rattus norvegicus
UNIPROT: P47820 not found.
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
release of a C-terminal dipeptide, oligopeptide-/-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II
Synonyms
angiotensin-converting enzyme, angiotensin converting enzyme, angiotensin converting enzyme inhibitor, angiotensin i-converting enzyme, angiotensin-converting-enzyme, angiotensin i converting enzyme, ace-1, kininase ii, angiotensin-i converting enzyme, angiotensin-converting enzyme-2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ANG I-converting enzyme
-
angiotensin I-converting enzyme
-
angiotensin 1 converting enzyme
-
-
-
-
angiotensin converting enzyme
angiotensin converting enzyme 1
-
-
angiotensin I converting enzyme
-
-
angiotensin I-converting enzyme
angiotensin-converting enzyme
angiotensin-I converting enzyme
-
-
carboxycathepsin
-
-
-
-
carboxypeptidase, dipeptidyl
-
-
-
-
CD143 antigen
-
-
-
-
Dipeptidyl carboxypeptidase
-
-
-
-
dipeptidyl carboxypeptidase I
-
-
-
-
endothelial cell peptidyl dipeptidase
-
-
-
-
kininase II
-
-
-
-
PDH
-
-
-
-
peptidase P
-
-
-
-
peptidyl dipeptidase
-
-
-
-
peptidyl dipeptidase A
-
-
-
-
peptidyl dipeptidase I
-
-
-
-
peptidyl dipeptidase-4
-
-
-
-
peptidyl dipeptide hydrolase
-
-
-
-
peptidyl-dipeptide hydrolase
-
-
-
-
peptidyldipeptide hydrolase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9015-82-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
angiotensin I + H2O
angiotensin II + His-Leu
show the reaction diagram
-
-
-
?
angiotensin I + H2O
angiotensin II + L-His-L-Leu
show the reaction diagram
-
-
-
?
hippuryl-His-Leu + H2O
hippuric acid + His-Leu
show the reaction diagram
-
-
-
?
N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine + H2O
?
show the reaction diagram
-
-
-
?
angiotensin I + H2O
angiotensin II + His-Leu
show the reaction diagram
angiotensin I + H2O
angiotensin II + L-His-L-Leu
show the reaction diagram
-
-
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
?
show the reaction diagram
-
i.e. substance P, lung and brain ACE cleave substance P via two pathways. In one pathway ACE first releases Gly-Leu-Met-NH2 and then dipeptides sequentially from the carboxyl terminus. The other first produces Leu-Met-NH2 and then releases dipeptides to leave substance P(1-5)
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly + Leu-Met-NH2
show the reaction diagram
bradykinin + H2O
?
show the reaction diagram
bradykinin + H2O
L-Phe-L-Arg + L-Ser-L-Pro + L-Arg-L-Pro-L-Pro-Gly-L-Phe
show the reaction diagram
-
-
-
-
?
bradykinin + H2O
Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe
show the reaction diagram
hippuryl-His-Leu + H2O
hippuric acid + His-Leu
show the reaction diagram
-
-
-
-
?
hippuryl-L-His-L-Leu + H2O
hippuric acid + L-His-L-Leu
show the reaction diagram
-
-
-
-
?
hippuryl-Phe-Arg + H2O
hippuric acid + Phe-Arg
show the reaction diagram
-
-
-
-
?
His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 + H2O
His-Lys-Thr-Asp-Ser-Phe-Val-Gly + Leu-Met-NH2
show the reaction diagram
-
i.e. substance K, degraded by striatal but not by lung enzyme
-
-
?
Luteinizing hormone-releasing hormone + H2O
?
show the reaction diagram
-
degraded by striatal and by lung enzyme
-
-
?
LVVYPWTQRY + H2O
LVVYPWTQ + RY + LVVY + PW + LVVYPW + TQ
show the reaction diagram
-
-
dipeptide TQ is unidentified. Sequential removal of dipeptides in three consecutive steps
?
N-(1-(S)-carboxy-3-phenylpropyl)-L-Ala-L-Pro + H2O
?
show the reaction diagram
-
i.e. MK-422
-
-
?
N-(1-(S)-carboxy-3-phenylpropyl)-L-Lys-L-Pro + H2O
?
show the reaction diagram
-
i.e. MK-522
-
-
?
physalaemin + H2O
pGlu-Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly + Leu-Met-NH2
show the reaction diagram
-
degraded by striatal and by lung enzyme
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
angiotensin I + H2O
angiotensin II + His-Leu
show the reaction diagram
-
-
-
?
angiotensin I + H2O
angiotensin II + L-His-L-Leu
show the reaction diagram
-
-
-
?
angiotensin I + H2O
angiotensin II + His-Leu
show the reaction diagram
-
i.e. DRVYIHPFHL
i.e. DRVYIHPF
-
?
angiotensin I + H2O
angiotensin II + L-His-L-Leu
show the reaction diagram
-
-
-
-
?
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O
?
show the reaction diagram
-
i.e. substance P, lung and brain ACE cleave substance P via two pathways. In one pathway ACE first releases Gly-Leu-Met-NH2 and then dipeptides sequentially from the carboxyl terminus. The other first produces Leu-Met-NH2 and then releases dipeptides to leave substance P(1-5)
-
-
?
bradykinin + H2O
?
show the reaction diagram
bradykinin + H2O
L-Phe-L-Arg + L-Ser-L-Pro + L-Arg-L-Pro-L-Pro-Gly-L-Phe
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na+
-
rats exposed to a high-sodium diet show an enhanced ACE activity
Zinc
-
0.3 mM ZnCl2 completely reverses the inhibition caused by 0.1 mM EDTA
Zn2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin gallate
-
5-(3,4,5-trihydroxyphenyl) 4-hydroxyvaleric acid
-
5-(3,4,5-trihydroxyphenyl)-gamma-valerolactone
the metabolite has a hypotensive effect in vivo
5-(3,5-dihydroxyphenyl) 4-hydroxyvaleric acid
-
5-(3,5-dihydroxyphenyl)-gamma-valerolactone
the metabolite has a hypotensive effect in vivo
SPB1
Bacillus subtilis crude lipopeptide biosurfactant, the biosurfactant displays a potent inhibition of ACE activity in vitro, IC50 is 1.37 mg/ml
-
1,10-phenanthroline
-
-
15B2
-
an inhibitor isolated from the culture broth of Actinomadura sp. No. 937ZE-1
aminoethyl-chitin
-
with 10%, 50%, and 90% deacetylation
angiotensin I
-
-
benzoyl-NHCOCH2CH(COOH)-Ala-Pro-OH
-
-
benzoyl-NHCOCH2CH(COOH)-Trp-Pro-OH
-
-
bradykinin
-
-
bradykinin potentating factor nonapeptide
-
-
-
bradykinin potentiator B
-
-
bradykinin-potentiator B
-
-
bradykinin-potentiator C
-
-
captopril
chitooligosaccharide derivatives
-
i.e. COS, chitosan derivatives, polycationic polymers comprised principally of glucosamine units, generated via either chemical or enzymatic hydrolysis of chitosan. ACE inhibitory activity of hetero-COS, derived from crab chitin, is dependent on the degree of deacetylation of chitosan
-
chitosan trimer
-
effective in lowering blood pressure
Co2+
-
testicular enzyme is inhibited, lung enzyme not
D-mannitol
-
antihypertensive effect in spontaneously hypertensive rats by oral administration
dexamethasone
-
markedly inhibits the plasma extravasion in the tracheal mucosa produced by substance P. The simultanous inhibition of neutral endopeptidase and angiotensin converting enzyme completely reverses the effect of dexamethasone on substance P-induced extravasion
dieckol
-
deribed from Ecklonia stolonifera
eckol
-
derived from Ecklonia stolonifera
EDENNPFYLR
-
-
enalapril
enalaprilat
-
inhibits ACE and the bradykinin degradation in vivo, which is reversed by insulin
enapril
-
-
enaprilat
-
-
ESIINF
-
the inhibitor produces an acute blood-pressure-lowering effect in spontaneously hypertensive rats upon a single oral administration
genistein
Gly-L-Ala-Hyp-Gly-L-Leu-Hyp-Gly-L-Pro
-
highest ACE-inhibitory activity
Gly-L-Ala-Hyp-Gly-L-Pro-L-Ala-Gly-L-Pro-Gly-Gly-L-Ile-Hyp-Gly-L-Glu-L-Arg-Gly
-
-
Gly-L-Ile-Hyp-Gly-L-Glu-L-Arg-Gly-L-Pro-L-Val-Gly-L-Pro-L-Ser-Gly
-
-
Gly-L-Leu-Hyp-Gly-L-Ser-L-Arg-Gly-L-Glu-L-Arg-Gly-L-Leu-Hyp-Gly
-
-
Gly-Phe-Hyp-Gly-Thr-Hyp-Gly-Leu-Hyp-Gly-Phe
-
inhibitory peptide derived from chicken breast muscle possesses hypotensive activity for spontaneously hypertensive rats
inhibitory peptides from rice dreg hydrolysate
-
significant antihypertensive action and no other side effects by oral administration in spontaneous hypertension rats
-
iodoMK-351A
-
-
-
IPPGVPYWT
-
-
isorhamnetin-3-beta-glucopyranoside
-
IC50: 0.4089 mM
kaempferol
-
dose-dependent inhibition, 46% inhibition at 0.1 mM
kaempferol-3-alpha-arabinopyranoside
-
IC50: 0.3928 mM
KRQKYDI
-
competitive inhibitor, the strongest inhibitor among reported troponin-originated peptides. The inhibhitor is slowly hydrolyzed by treatment with angiotensin I-converting enzyme. When KRQKYDI is administered orally to spontaneously hypertensive rats at a dose of 10 mg/kg, a temporary antihypertensive activity is observed at 3 and 6 h after administration
L-681,176
-
purification of the inhibitor found in the culture filtrate of Streptomyces sp. MA 5143
Leu-Gln-Pro
-
competitive
Leu-Lys-Tyr
-
competitive
Leu-Val-Tyr
-
competitive
lisinopril
N-[(1S)-1-(ethoxycarbonyl)-3-phenylpropyl]-L-alanyl-L-proline
N-[(S)-1-Carboxy-3-phenylpropyl]-L-Ala-L-Pro
-
-
NaCl
-
concentration dependent inhibition of hippuryl-His-Leu between 0 and 100 mM
nicotianamine
peimisine
-
IC50: 0.5265 mM
perindopril
perindoprilat
-
strong inhibitor
phlorofucofuroeckol A
-
derived from Ecklonia stolonifera
phlorotannins
-
e.g. from Ahnfeltiopsis flabelliformis, Ecklonia cava, Ecklonia stolonifera, Pelvetia siliqousa, and Undaria pinnatifida, phenolic compounds formed by the polymerization of phloroglucinol or defined as 1,3,5-trihydroxybenzene monomer units and biosynthesized through the acetate-malonate pathway. They are highly hydrophilic components with a wide range of molecular sizes ranging between 126-650 kDa. A closed ring dibenzo-1,4-dioxin moiety may be crucial for ACE inhibitory effects
-
quercetin 3-O-alpha-(6''-p-coumaroylglucosyl-beta -1,2-rhamnoside)
-
IC50: 0.351 mM
quercetin-3-beta-glucopyranoside
-
IC50: 0.7088 mM
quercetin-3-O-alpha-(6''-caffeoylglucosyl-beta-1,2-rhamnoside)
-
IC50: 0.1589 mM
quinapril
ramipril
S-acetylcaptopril
-
-
sardine peptide
-
a protein hydrolysate derived from muscles of sardines, inhibits in vivo and reduces the blood glucose level, but not the plasma insulin level
-
Teprotide
-
i.e. SQ 20881
trandolapril
-
the angiotensin-converting enzyme inhibitor has no significant effect on apoptosis induced via endotoxic shock with Escherichia coli lipopolysaccharides
Trp-Pro-Glu-Ala-Ala-Glu-Leu-Met-Met-Glu-Val-Asp-Pro
-
noncompetitive inhibitor.The peptide has an antihypertensive effect according to the time-course measurement after oral administration to spontaneously hypertensive rats. Maximal reduction is detected 3 h after oral administration at a dose of 10 mg/kg of body weight
Val-Lys-Lys-Val-Leu-Gly-Asn-Pro
-
the angiotensin-I converting enzyme inhibitory peptide derived from porcine skeletal muscle myosin is a noncompetitive inhibitor that is slowly hydrolyzed by angiotensin-I converting enzyme. At the dose of 10 mg/kg, this peptide shows antihypertensive activity after a maximum of 3 h of administration
verticine
-
IC50: 0.3128 mM
verticinone
-
IC50: 0.165 mM
YRGGLEPINF
-
the inhibitor produces an acute blood-pressure-lowering effect in spontaneously hypertensive rats upon a single oral administration
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
angiotensin I
-
pH 7.5, 37Ā°C, enzyme from brain, 200 mM NaCl
1.2 - 4
hippuryl-His-Leu
0.5 - 1.8
hippuryl-Phe-Arg
0.014 - 0.033
LVVYPWTQRY