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Information on EC 3.4.14.4 - dipeptidyl-peptidase III and Organism(s) Drosophila melanogaster and UniProt Accession Q9VHR8

for references in articles please use BRENDA:EC3.4.14.4
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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q9VHR8 not found.
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.
Synonyms
dpp iii, dipeptidyl peptidase iii, nudt3, enkephalinase b, dppiii, dpp-iii, dipeptidyl aminopeptidase iii, dipeptidyl-peptidase iii, dipeptidylpeptidase iii, dipeptidyl-peptide hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dipeptidyl aminopeptidase III
-
dipeptidyl aminopeptidase III
-
-
-
-
dipeptidyl arylamidase III
-
-
-
-
dipeptidyl-peptide hydrolase
-
-
-
-
dipeptidylpeptidase III
-
-
-
-
enkephalinase B
-
-
-
-
peptidase, dipeptidyl, III
-
-
-
-
red cell angiotensinase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
77464-87-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Arg-Tyr-Leu-Pro-Thr + H2O
Arg-Tyr + Leu-Pro-Thr
show the reaction diagram
-
-
-
?
proctolin + H2O
Arg-Tyr + Leu-Pro-Thr
show the reaction diagram
-
-
-
?
Tyr-Gly-Gly-Phe-Met + H2O
?
show the reaction diagram
-
-
-
?
Arg-Tyr-Leu-Pro-Thr + H2O
Arg-Tyr + Leu-Pro-Thr
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
516% activity in the presence of 0.1 mM Co2+, 758% activity in the presence of 1 mM Co2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EGTA
96% inhibition in the presence of 1 mM EGTA, but 110% activity in the presence of the two inhibitors 1 mM EGTA and 1 mM Zn2+
tynorphin
Val-Val-Tyr-Pro-Trp, 10 microM, 99% inhibition
Zn2+
19% inhibition in the presence of 0.1 mM Zn2+, 99.8% inhibition in the presence of 1 mM Zn2+
tynorphin
-
50% inhibition at 0.00062 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0419
Tyr-Gly-Gly-Phe-Met
-
0.004
Arg-Tyr-Leu-Pro-Thr
-
25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DPP3_DROME
786
0
89194
Swiss-Prot
Mitochondrion (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
82000
SDS-PAGE and Western blot
89000
SDS-PAGE and Western blot
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 82000-86000, two protein bands, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
-
six potential glycosylation sites, but no glycosylation detectable
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Hi-Trap Q Sepharose chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in S2 cells
expression in Schneider S2 cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mazzocco, C.; Fukasawa, K.M.; Auguste, P.; Puiroux J.
Characterization of a functionally expressed dipeptidyl aminopeptidase III from Drosophila melanogaster
Eur. J. Biochem.
270
3074-3082
2003
Drosophila melanogaster
Manually annotated by BRENDA team
Mazzocco, C.; Gillibert-Duplantier, J.; Neaud, V.; Fukasawa, K.M.; Claverol, S.; Bonneu, M.; Puiroux, J.
Identification and characterization of two dipeptidyl-peptidase III isoforms in Drosophila melanogaster
FEBS J.
273
1056-1064
2006
Drosophila melanogaster (Q9VHR8), Drosophila melanogaster
Manually annotated by BRENDA team
Isaac, R.; Bland, N.; Shirras, A.
Neuropeptidases and the metabolic inactivation of insect neuropeptides
Gen. Comp. Endocrinol.
162
8-17
2009
Blaberus craniifer, Drosophila melanogaster (Q9VHR8)
Manually annotated by BRENDA team