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EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Hydrolysis of Met-/-Xaa dipeptides.
Synonyms
dipeptidase M, methionyl dipeptidase,
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methionyl dipeptidase
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Hydrolysis of Met-/-Xaa dipeptides.
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hydrolysis of peptide bond
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dipeptide hydrolase
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Ala-Ala + H2O
Ala + Ala
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ir
Ala-Leu + H2O
Ala + Leu
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ir
ethionyl-Ala + H2O
ethionine + Ala
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-
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ir
Gly-Gly + H2O
Gly + Gly
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ir
Met-Glu + H2O
Met + Glu
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cleaved at about 10% of the maximal rate
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?
Met-Met + H2O
Met + Met
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ir
norleucyl-Ala + H2O
norleucine + Ala
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ir
Ala-Met + H2O
Ala + Met
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ir
Ala-Met + H2O
Ala + Met
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ir
Leu-Ala + H2O
Leu + Ala
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ir
Leu-Ala + H2O
Leu + Ala
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ir
Met-Ala + H2O
Met + Ala
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ir
Met-Ala + H2O
Met + Ala
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cleaved most rapidly
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?
Met-Ala + H2O
Met + Ala
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best substrate
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ir
Met-amino acid + H2O
?
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?
Met-amino acid + H2O
?
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?
Met-Gly + H2O
Met + Gly
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ir
Met-Gly + H2O
Met + Gly
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cleaved at about 60% of the maximal rate
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?
Met-Ile + H2O
Met + Ile
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ir
Met-Ile + H2O
Met + Ile
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cleaved at about 10% of the maximal rate
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?
Met-Ile + H2O
Met + Ile
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ir
Met-Leu + H2O
Met + Leu
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ir
Met-Leu + H2O
Met + Leu
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cleaved at about 60% of the maximal rate
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?
Met-Leu + H2O
Met + Leu
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ir
Met-Ser + H2O
Met + Ser
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ir
Met-Ser + H2O
Met + Ser
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cleaved most rapidly
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?
Met-Ser + H2O
Met + Ser
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ir
Met-Thr + H2O
Met + Thr
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ir
Met-Thr + H2O
Met + Thr
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cleaved at about 60% of the maximal rate
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?
Met-Thr + H2O
Met + Thr
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ir
Met-Val + H2O
Met + Val
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?
Met-Val + H2O
Met + Val
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cleaved at about 10% of the maximal rate
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?
Ser-Ala + H2O
Ser + Ala
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ir
Ser-Ala + H2O
Ser + Ala
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ir
Thr-Ala + H2O
Thr + Ala
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ir
Thr-Ala + H2O
Thr + Ala
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ir
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Met-amino acid + H2O
?
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?
Met-amino acid + H2O
?
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?
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Co2+
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52% of activity with manganese
Fe2+
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21% of activity with manganese
Zn2+
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20% of activity with manganese
Mn2+
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also protects against heat inactivation
Mn2+
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is the only divalent cation to restore activity following EDTA treatment
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iodoacetate
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100% inhibition at 10 mM, 10 mM mercaptoethanol restores activity
PCMB
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inhibition is reversed by 2-mercaptoethanol
EDTA
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Mn2+ may restore enzyme activity after EDTA treatment
p-chloromercuribenzoate
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100% inhibition at 0.1 mM
p-chloromercuribenzoate
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100% inhibition at 1 mM
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0.25 - 0.31
Met-Ala
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0.42 - 1.02
Met-Ile
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0.57 - 0.6
Met-Ser
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7.8 - 9
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7.8 - 9
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substrate Met-Ala
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5.5 - 9.5
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substrate Met-Ala
5.5 - 9.5
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substrate Met-Ala
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brenda
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brenda
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110000 - 114000
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gel filtration
47000
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2 * 47000, SDS-PAGE
93000 - 95000
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gel filtration
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dimer
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2 * 47000, SDS-PAGE
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50
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below, 30 min, Mn2+ protects against heat inactivation
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unstable in absence of metal ions and reducing agents. Mn2+ is the best cation for maintaining stability
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4°C, 10 mM imidazole buffer, pH 7.2, 2 mg/ml bovine serum albumin, 30 days, 50% loss of activity
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Johnson, G.L.; Brown, J.L.
Partial purification and characterization of two peptidases from Neurospora crassa
Biochim. Biophys. Acta
370
530-540
1974
Neurospora crassa
brenda
Brown, J.L.
Purification and properties of dipeptidase M from Escherichia coli B
J. Biol. Chem.
248
409-416
1973
Escherichia coli
brenda
Woessner, J.F.
Met-X dipeptidase
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
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1018-1020
2004
Escherichia coli
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brenda
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3.4.13.12 Met-Xaa dipeptidase
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