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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys
Synonyms
apb, arylamidase, aminopeptidase b, arginine aminopeptidase, cytosol aminopeptidase, arginyl aminopeptidase, aminopeptidase-b, dap bii, l-rap, arginine-aminopeptidase,
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double-zinc aminopeptidase
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aminopeptidase, arginine
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arginine aminopeptidase
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arginyl aminopeptidase
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Cl--activated arginine aminopeptidase
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cytosol aminopeptidase
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L-arginine aminopeptidase
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-
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L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
low activity
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-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
best substrate
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-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
-
-
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?
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
low activity
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?
additional information
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additional information
?
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substrate specificity, 4-nitroanilides of Gly, Glu, Asp, Phe, Pro, and Ala-Pro are poor substrates, overview
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?
additional information
?
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substrate specificity, 4-nitroanilides of Gly, Glu, Asp, Phe, Pro, and Ala-Pro are poor substrates, overview
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?
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Zn2+
zinc-metalloaminopeptidase, 2 Zn2+ per enzyme molecule
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1,10-phenanthroline
complete inhibition, reversible by Zn2+
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10
L-Ala-4-nitroanilide
pH 8.0, 30°C
0.6
L-Arg-4-nitroanilide
pH 8.0, 30°C
3.6
L-Leu-4-nitroanilide
pH 8.0, 30°C
0.85
L-Lys-4-nitroanilide
pH 8.0, 30°C
19
L-Val-4-nitroanilide
pH 8.0, 30°C
additional information
additional information
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additional information
additional information
kinetics
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additional information
additional information
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kinetics
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0.37
L-Ala-4-nitroanilide
pH 8.0, 30°C
34
L-Arg-4-nitroanilide
pH 8.0, 30°C
55
L-Leu-4-nitroanilide
pH 8.0, 30°C
10
L-Lys-4-nitroanilide
pH 8.0, 30°C
0.43
L-Val-4-nitroanilide
pH 8.0, 30°C
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64
purified recombinant enzyme
additional information
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additional information
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ywad gene
SwissProt
brenda
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80
20 min, purified recombinant enzyme, stable
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recombinant enzyme 5fold from Escherichia coli by ion exchange chromatography
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gene ywad, overexpression in Escherichia coli
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Fundoiano-Hershcovitz, Y.; Rabinovitch, L.; Shulami, S.; Reiland, V.; Shoham, G.; Shoham, Y.
The ywad gene from Bacillus subtilis encodes a double-zinc aminopeptidase
FEMS Microbiol. Lett.
243
157-163
2005
Bacillus subtilis (P25152), Bacillus subtilis
brenda
3.4.11.6 aminopeptidase B
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