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Information on EC 3.4.11.25 - beta-peptidyl aminopeptidase
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EC Tree
3.4.11.25 beta-peptidyl aminopeptidaseSpecify your search results
Word Map
- 3.4.11.25
- synthesis
-
beta-amino
- beta-peptides
-
alpha-amino
- rrna
-
l-configuration
- preproteins
- aminopeptidases
-
anthropi
-
ochrobactrum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids
Synonyms
beta-peptidyl aminopeptidase, 3-2w4 bapa, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
3-2W4 BapA
Y2 BapA
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids
-
-
-
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
2 beta-homoGly 4-nitroanilide
beta-homoGly-beta-homoGly 4-nitroanilide + 4-nitroaniline
-
product of mutant S237C, synthesized efficiently without degradation
-
?
beta-homoGly 4-nitroanilide + H2O
beta-homoGly + 4-nitroaniline
-
wild-type enzyme shows beta-homoGly-beta-homoGly-4-nitroanilide synthetic activity, but the product vanishes in a few min and converts to free beta-homoGly
-
?
beta-homoVal-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
insulin + rac-3-aminobutanamide
?
enatioselectivity for beta-alanine is more than 300:1
-
-
r
Val-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
only the N-terminal beta-homoVal is released. Preferred substrate
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
1.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
1.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
preferred substrate
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
preferred substrate
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
3.4% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
0.3% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
0.4% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
12% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
0.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
15% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
13% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
1.6% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
1.3% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
7% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
27% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
preferred substrate
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
15% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
?
additional information
?
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
?
additional information
?
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
additional information
-
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Pefabloc SC
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
Pefabloc SC
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
additional information
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
-
additional information
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
-
additional information
no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
additional information
-
no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
additional information
-
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
9
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
39
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
12 - 60
beta-homoAla-beta-homoLeu