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Synonyms
erap1, aminopeptidase i, endoplasmic reticulum aminopeptidase 1, leucine-aminopeptidase, eraap, endoplasmic reticulum aminopeptidase, a-lap, arts-1, aminopeptidase 1, lapase,
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Ala 4-nitroanilide + H2O
Ala + 4-nitroaniline
-
-
-
?
Arg 4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
-
?
Cystinyl 4-nitroanilide + H2O
Cystine + 4-nitroaniline
-
-
-
?
Glu 4-nitroanilide + H2O
Glu + 4-nitroaniline
-
-
-
?
Gly 4-nitroanilide + H2O
Gly + 4-nitroaniline
-
-
-
?
L-Ala-Gly + H2O
L-Ala + Gly
-
-
-
-
?
L-Ala-Gly-Gly + H2O
?
-
-
-
-
?
L-Ala-Gly-Gly ethyl ester + H2O
L-Ala + Gly-Gly ethyl ester
-
-
-
-
?
L-Ala-L-Asp-L-Phe methyl ester + H2O
?
-
-
-
-
?
L-Ala-L-Leu + H2O
L-Ala + L-Leu
-
-
-
-
?
L-Ala-L-Thr + H2O
L-Ala + L-Thr
-
-
-
-
?
L-Ala-L-Thr-Gly methyl ester + H2O
?
-
-
-
-
?
L-Ala-L-Thr-L-Ala + H2O
?
-
-
-
-
?
L-Ala-L-Thr-L-Ala methyl ester + H2O
?
-
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
-
preferred substrate
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
L-Leu-Gly + H2O
L-Leu + Gly
-
-
-
-
?
L-Leu-Gly-Gly + H2O
?
-
-
-
-
?
L-Leu-Gly-L-Leu + H2O
?
-
-
-
-
?
L-Leu-L-Ala + H2O
L-Leu + L-Ala
-
-
-
-
?
L-Leu-L-Leu + H2O
L-Leu + L-Leu
-
-
-
-
?
L-Lys-2-naphthylamide + H2O
L-Lys + 2-naphthylamine
-
low activity
-
-
?
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
-
low activity
-
-
?
L-Phe-Gly + H2O
L-Phe + Gly
-
-
-
-
?
L-Phe-Gly-Gly + H2O
?
-
-
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
best substrate
-
-
?
Leu-beta-naphthylamide + H2O
Leu + beta-naphthylamine
-
-
-
-
?
Lys 4-nitroanilide + H2O
Lys + 4-nitroaniline
-
-
-
?
Met 4-nitroanilide + H2O
Met + 4-nitroaniline
-
-
-
?
Phe 4-nitroanilide + H2O
Phe + 4-nitroaniline
-
-
-
?
Pro 4-nitroanilide + H2O
Pro + 4-nitroaniline
-
-
-
?
S-Benzoyl-Cys 4-nitroanilide + H2O
S-Benzoyl-Cys + 4-nitroaniline
-
-
-
?
Val 4-nitroanilide + H2O
Val + 4-nitroaniline
-
-
-
?
additional information
?
-
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
preferred substrate
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
-
?
additional information
?
-
-
overview, peptidyl substrates, Leu and other hydrophobic amino acids are best substrates, Gly and other charged amino acids are split off more slowly
-
-
?
additional information
?
-
-
no hydrolyis of succinyl-Ala-Ala-Pro-Phe 4-nitroanilide, succinyl-Ala-Ala-Pro-Leu 4-nitroanilide, succinyl-Ala-Ala-Val- Ala 4-nitroanilide, benzyloxycarbonyl-Arg-Arg 4-nitroanilide, benzyloxycarbonyl-Gly-Pro 4-nitroanilide, benzyloxycarbonyl-Pro-Phe-Arg 4-nitroanilide, acetyl-Arg-Gly-Gly-Met 4-nitroanilide, benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide, benzoyl-Tyr 4-nitroanilide, benzyloxycarbonyl-Lys-Arg 4-nitroanilide
-
-
?
additional information
?
-
-
the glycosylated Ape2 aminopeptidase might be responsible for uptake of hydrophobic peptides, especially of leucine N-terminal peptides
-
-
?
additional information
?
-
-
the enzyme cleaves internal peptide binds in di- and tripeptides with preference for hydrophobic N-terminal amino acids
-
-
?
additional information
?
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-
the enzyme prefers substrates with N-terminal leucine or another hydrophobic amino acid residue, and is less active with Gly or charged amino acids at the P1 position
-
-
?
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100000
-
dimeric form, sucrose density gradient centrifugation
140000
-
glycosylated active enzyme form, gel filtration
320000
-
hexameric form, sucrose density gradient centrifugation
400000
-
beyond 400000 Da, gel filtration
45000
-
x * 45000, SDS-PAGE
50000
-
12 * 50000, the active enzyme is a homododecameric complex of about 600 kDa, and dissociation into two hexamers leads to loss of enzymatic activity
51000
-
12 * 51000, SDS-PAGE
52000
-
gel filtration, non-denaturing PAGE
610000
-
sucrose density gradient centrifugation
85000 - 90000
-
unglycosylated active enzyme form, gel filtration
97634
-
1 * 97634, amino acid sequence calculation
53000
-
12 * 53000, single type of subunit, SDS-PAGE, cyanogen bromide cleavage
53000
-
6 * 53000, single type of subunit, SDS-PAGE, cyanogen bromide cleavage
53000
-
12 * 53000, active enzyme
640000
-
-
640000
-
dodecameric form, sucrose density gradient centrifugation
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Klionsky, D.J.
Nonclassical protein sorting to the yeast vacuole
J. Biol. Chem.
273
10807-10810
1998
Saccharomyces cerevisiae
brenda
Martinez, E.; Jimenez, M.A.; Segui-Real, B.; Vandekerckhove, J.; Sandoval, I.V.
Folding of the presequence of yeast pAPI into an amphipathic helix determines transport of the protein from the cytosol to the vacuole
J. Mol. Biol.
267
1124-1138
1997
Saccharomyces cerevisiae
brenda
Kim, J.; Scott, S.V.; Oda, M.N.; Klionsky, D.J.
Transport of a large oligomeric protein by the cytoplasm to vacuole protein targeting pathway
J. Cell Biol.
137
609-618
1997
Saccharomyces cerevisiae
brenda
Scott, S.V.; Baba, M.; Ohsumi, Y.; Klionsky, D.J.
Aminopeptidase I is targeted to the vacuole by a nonclassical vesicular mechanism
J. Cell Biol.
138
37-44
1997
Saccharomyces cerevisiae
brenda
Oda, M.N.; Scott, S.V.; Hefner-Gravink, A.; Caffarelli, A.C.; Klionsky, D.J.
Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I
J. Cell Biol.
132
999-1010
1996
Saccharomyces cerevisiae
brenda
Scott, S.V.; Klionsky, D.J.
In vitro reconstitution of cytoplasm to vacuole protein targeting in yeast
J. Cell Biol.
3131
1727-1735
1995
Saccharomyces cerevisiae
brenda
Segui-Real, B.; Martinez, M.; Sandoval, I.V.
Yeast aminopeptidase I is post-translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N-terminal extension
EMBO J.
14
5476-5484
1995
Saccharomyces cerevisiae
brenda
Tisljar, U.; Wolf, D.H.
Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI
FEBS Lett.
322
191-196
1993
Saccharomyces cerevisiae
brenda
Hetz, G.; Rhm, K.H.
Interaction of chloride with yeast aminopeptidase I. Equilibrium binding studies
Biol. Chem. Hoppe-Seyler
368
63-66
1987
Saccharomyces cerevisiae
brenda
Rhm., K.H.
Chloride as allosteric effector of yeast aminopeptidase I
Arch. Biochem. Biophys.
239
216-225
1985
Saccharomyces cerevisiae
brenda
Rhm., K.H.
Metal binding to yeast aminopeptidase I
Eur. J. Biochem.
146
633-639
1985
Saccharomyces cerevisiae
brenda
Schwencke, J.; Moustacchi, E.
Proteolytic activities in yeast after UV irradiation. I. Variation in proteinase levels in repair proficient Rad+ strains
Mol. Gen. Genet.
185
290-295
1982
Saccharomyces cerevisiae
brenda
Lffler, H.G.; Rhm.K.H.
Comparative studies on the dodecameric and hexameric forms of yeast aminopeptidase I
Z. Naturforsch. C
34
381-386
1979
Saccharomyces cerevisiae
-
brenda
Metz, G.; Marx, R.; Rhm, K.H.
The quarternary structure of yeast aminopeptidase I 1. Molecular forms and subunit size
Z. Naturforsch. C
32
929-937
1977
Saccharomyces cerevisiae
brenda
Metz, G.; Rhm.K.H.
Yeast aminopeptidase I. Chemical composition and catalytic properties
Biochim. Biophys. Acta
429
933-949
1976
Saccharomyces cerevisiae
brenda
Cueva, R.; Garcia-Alvarez, N.; Suarez-Rendueles, P.
Yeast vacuolar aminopeptidase yscI. Isolation and regulation of the APE1 (LAP4) structural gene
FEBS Lett.
259
125-129
1989
Saccharomyces cerevisiae (P14904), Saccharomyces cerevisiae
brenda
Caprioglio, D.R.
Yeast aminopeptidases Ape2, Aap1' and Yin7
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds)
1
316-318
2004
Saccharomyces cerevisiae
-
brenda
Suarez-Rendueles, P.; Bordallo, J.; Cueva, R.
Aminopeptidase I
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds)
1
940-941
2004
Saccharomyces cerevisiae
-
brenda
Andrei-Selmer, C.; Knuppel, A.; Satyanarayana, C.; Heese, C.; Schu, P.V.
A new class of mutants deficient in dodecamerization of aminopeptidase 1 and vacuolar transport
J. Biol. Chem.
276
11606-11614
2001
Saccharomyces cerevisiae
brenda
Adachi, W.; Suzuki, N.N.; Fujioka, Y.; Suzuki, K.; Ohsumi, Y.; Inagaki, F.
Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway
Acta Crystallogr. Sect. F
63
200-203
2007
Saccharomyces cerevisiae
brenda
Schu, P.
Aminopeptidase I enzymatic activity
Methods Enzymol.
451
67-78
2008
Saccharomyces cerevisiae
brenda
Adamis, P.; Mannarino, S.; Riger, C.; Duarte, G.; Cruz, A.; Pereira, M.; Eleutherio, E.
Lap4, a vacuolar aminopeptidase I, is involved in cadmium-glutathione metabolism
Biometals
22
243-249
2009
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
brenda