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Information on EC 3.4.11.2 - membrane alanyl aminopeptidase and Organism(s) Plutella xylostella and UniProt Accession P91887

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.2 membrane alanyl aminopeptidase
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Select one or more organisms in this record: ?
This record set is specific for:
Plutella xylostella
UNIPROT: P91887 not found.
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Word Map
The taxonomic range for the selected organisms is: Plutella xylostella
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Release of an N-terminal amino acid, Xaa-/-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide
Synonyms
aminopeptidase, aminopeptidase n, dipeptidase, aminopeptidase m, alanine aminopeptidase, apn/cd13, gp150, aminopeptidase-n, anpep, alanyl aminopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alanine aminopeptidase
-
-
-
-
alanine-specific aminopeptidase
-
-
-
-
alanyl aminopeptidase
-
-
-
-
Alpha-aminoacylpeptide hydrolase
-
-
-
-
amino-oligopeptidase
-
-
-
-
aminopeptidase M
-
-
-
-
aminopeptidase M II
-
-
-
-
aminopeptidase N
aminopeptidase, microsomal
-
-
-
-
APN2
-
-
-
-
CD13
-
-
-
-
CryIA(C) receptor
-
-
-
-
GP 130
-
-
-
-
GP150
-
-
-
-
L-alanine aminopeptidase
-
-
-
-
Leukemia antigen CD13
-
-
-
-
Lys-AP
-
-
-
-
Lysyl aminopeptidase
-
-
-
-
Membrane glycoprotein H11
-
-
-
-
Membrane protein p161
-
-
-
-
Microsomal aminopeptidase
-
-
-
-
Myeloid plasma membrane glycoprotein CD13
-
-
-
-
particle-bound aminopeptidase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
CAS REGISTRY NUMBER
COMMENTARY hide
9054-63-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aminoacyl peptide
?
show the reaction diagram
-
-
-
?
insecticidal crystal protein
?
show the reaction diagram
from Bacillus thuringensis, binds to aminopeptidase N
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
aminoacyl peptide
?
show the reaction diagram
-
-
-
?
insecticidal crystal protein
?
show the reaction diagram
from Bacillus thuringensis, binds to aminopeptidase N
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
enzyme contains a consensus zinc-binding/gluzincin motif HEXXHX18E
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Bacillus thuringiensis Cry1Aa toxin
specific binding to APN isoforms, enzyme is the major toxin receptor in the midgut of insects
-
Bacillus thuringiensis Cry1Ab toxin
specific binding to APN isoforms, enzyme is the major toxin receptor in the midgut of insects
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
brush border membrane
Manually annotated by BRENDA team
-
brush border
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AMPN_PLUXY
946
0
106578
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
-
recombinant isozyme APN1 from Sf9 cells
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
side-chain modification
-
glycoprotein
4 putative N-glycosylation and 22 O-glycosylation sites
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partially by preparation of brush border membrane vesicles from whole larvae
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli, which is more difficult for the full-length enzyme as for fragments, successfull expression of isozyme APN1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, but not of isozyme APN2, transient expression of isozyme APN1 on the cell surface in Sf21 cells under control of the inducible Drosophila HSP70 promoter
-
isozyme PxAPN3, DNA and amino acid sequence determination and analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Denholf, P.; Hendrickx, K.; Van Damme, J.; Jansens, S.; Peferoen, M.; Degheele, D.; Van Rie, J.
Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N enzymes related to Bacillus thuringiensis toxin-binding proteins
Eur. J. Biochem.
248
748-761
1997
Manduca sexta (P91885), Manduca sexta, Plutella xylostella (P91887), Plutella xylostella
Manually annotated by BRENDA team
Nakanishi, K.; Yaoi, K.; Nagino, Y.; Hara, H.; Kitami, M.; Atsumi, S.; Miura, N.; Sato, R.
Aminopeptidase N isoforms from the midgut of Bombyx mori and Plutella xylostella - their classification and the factors that determine their binding specificity to Bacillus thuringiensis Cry1A toxin
FEBS Lett.
519
215-220
2002
Bombyx mori (Q8T7M0), Bombyx mori, Plutella xylostella (Q9U8B0), Plutella xylostella
Manually annotated by BRENDA team
Adang, M.J.
Insect aminopeptidase N
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
296-299
2004
Bombyx mori, Drosophila melanogaster, Heliothis virescens, Manduca sexta, Plutella xylostella, Tenebrio molitor, Epiphyas postvittana, Helicoverpa punctigera
-
Manually annotated by BRENDA team